diff --git a/reflex/state.py b/reflex/state.py
index 5116069ab6..1b5eda865a 100644
--- a/reflex/state.py
+++ b/reflex/state.py
@@ -93,6 +93,9 @@ def __init__(self, *args, parent_state: State | None = None, **kwargs):
             *args: The args to pass to the Pydantic init method.
             parent_state: The parent state.
             **kwargs: The kwargs to pass to the Pydantic init method.
+
+        Raises:
+            ValueError: If a substate class shadows another.
         """
         kwargs["parent_state"] = parent_state
         super().__init__(*args, **kwargs)
@@ -103,7 +106,13 @@ def __init__(self, *args, parent_state: State | None = None, **kwargs):
 
         # Setup the substates.
         for substate in self.get_substates():
-            self.substates[substate.get_name()] = substate(parent_state=self)
+            substate_name = substate.get_name()
+            if substate_name in self.substates:
+                raise ValueError(
+                    f"The substate class '{substate_name}' has been defined multiple times. Shadowing "
+                    f"substate classes is not allowed."
+                )
+            self.substates[substate_name] = substate(parent_state=self)
         # Convert the event handlers to functions.
         self._init_event_handlers()
 
diff --git a/tests/conftest.py b/tests/conftest.py
index 026ae47bcb..895954afad 100644
--- a/tests/conftest.py
+++ b/tests/conftest.py
@@ -584,3 +584,23 @@ def reassign_mutables(self):
             self.test_set = {1, 2, 3, 4, "five"}
 
     return MutableTestState()
+
+
+@pytest.fixture
+def duplicate_substate():
+    """Create a Test state that has duplicate child substates.
+
+    Returns:
+        The test state.
+    """
+
+    class TestState(rx.State):
+        pass
+
+    class ChildTestState(TestState):  # type: ignore # noqa
+        pass
+
+    class ChildTestState(TestState):  # type: ignore # noqa
+        pass
+
+    return TestState
diff --git a/tests/test_state.py b/tests/test_state.py
index 8240c961ca..763d36a6dd 100644
--- a/tests/test_state.py
+++ b/tests/test_state.py
@@ -1583,3 +1583,8 @@ def assert_custom_dirty():
     assert_custom_dirty()
     mutable_state._be_custom.custom.bar = "baz"
     assert_custom_dirty()
+
+
+def test_duplicate_substate_class(duplicate_substate):
+    with pytest.raises(ValueError):
+        duplicate_substate()