-
Notifications
You must be signed in to change notification settings - Fork 81
Commit
This commit does not belong to any branch on this repository, and may belong to a fork outside of the repository.
- Loading branch information
Showing
1 changed file
with
87 additions
and
0 deletions.
There are no files selected for viewing
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
Original file line number | Diff line number | Diff line change |
---|---|---|
@@ -0,0 +1,87 @@ | ||
|
||
|
||
//! A module that provides an easier way to work with SAM flags. | ||
//! It achieves this by providing a struct (`SamFlag`) with associated constants representing | ||
//! flags, and the `check_flag()` function that allows testing if specific flags are set or not set. | ||
//! | ||
//! For example, the following code tests if the read flag has the FIRST_IN_PAIR flag set and the MATE_UNMAPPED flag not set: | ||
//! ``` | ||
//! use rust_htslib::bam::{SamFlag, check_flag}; | ||
//! # let read_flag = record.flag(); in general this is the way to obtian a flag. | ||
//! let read_flag = 64; | ||
//! assert_eq!(check_flag(read_flag, SamFlag::FIRST_IN_PAIR, SamFlag::MATE_UNMAPPED), true); | ||
//! ``` | ||
|
||
/// | ||
/// This structure contains constants representing SAM flag values as u16. | ||
/// Using this structure incurs no runtime cost. | ||
/// | ||
/// ``` | ||
/// use rust_htslib::bam::{SamFlag}; | ||
/// to get the value of a flag representing a read paired, and reversly mapped. | ||
/// let flag = SamFlag::PAIRED + SamFlag::READ_RERVERSE; | ||
/// | ||
/// ``` | ||
pub struct SamFlag; | ||
|
||
|
||
impl SamFlag { | ||
pub const PAIRED: u16 = 1; | ||
pub const PROPERLY_PAIRED: u16 = 2; | ||
pub const READ_UNMAPPED: u16 = 4; | ||
pub const MATE_UNMAPPED: u16 = 8; | ||
pub const READ_RERVERSE: u16 = 16; | ||
pub const MATE_REVERSE: u16 = 32; | ||
pub const FIRST_IN_PAIR: u16 = 64; | ||
pub const SECOND_IN_PAIR: u16 = 128; | ||
pub const NOT_PRIMARY_ALN: u16 = 256; | ||
pub const FAIL_QC: u16 = 512; | ||
pub const DUPLICATE: u16 = 1024; | ||
pub const SUPPLEMENTARY: u16 = 2048; | ||
} | ||
|
||
|
||
pub fn check_flag(flag: u16, in_: u16, not_in: u16) -> bool { | ||
|
||
//! This function uses bitwise operations to test if flags are set or not. | ||
//! | ||
//! # Arguments | ||
//! | ||
//! * `flag`: u16 - The record flag you want to test | ||
//! * `in_`: u16 - The flags you want to check if they are set (use 0 for no test) | ||
//! * `not_in`: u16 - The flags you want to check if they are not set (use 0 for no test) | ||
//! | ||
//! # Usage: | ||
//! example: let test if a flag is both paired and fisrt in pair | ||
//! ``` | ||
//! use rust_htslib::bam::{SamFlag, check_flag}; | ||
//! let read_flag = 18 | ||
//! assert_eq!(check_flag(read_flag, SamFlag::PAIRED +SamFlag::FIRST_IN_PAIR, 0), true); | ||
//! ``` | ||
//! let test that the read is mapped. READ_UNMAPPED | ||
//! ``` | ||
//! use rust_htslib::bam::{SamFlag, check_flag}; | ||
//! let read_flag = 18 | ||
//! assert_eq!(check_flag(read_flag, 0, SamFlag::READ_UNMAPPED), true); | ||
//! ``` | ||
//! | ||
//! Finally let do a more complexe real example test: | ||
//! ``` | ||
//! use rust_htslib::bam::{SamFlag, check_flag}; | ||
//! let read_flag = 19 | ||
//! assert_eq!(check_flag(read_flag, SamFlag::PAIRED + SamFlag::PROPERLY_PAIRED + SamFlag::READ_RERVERSE , SamFlag::READ_UNMAPPED + SamFlag::MATE_UNMAPPED), true); | ||
//! ``` | ||
//! | ||
//binary flag check | ||
//assert that: - in_ is in n | ||
// - not_in is not in n | ||
// bitwise operation | ||
if (not_in & flag) != 0 { | ||
return false; | ||
} | ||
if (in_ & flag) != in_ { | ||
return false; | ||
} | ||
return true; | ||
} |