From 800d2143545f9f4a6cb0036e4ba25b2cdc33dac1 Mon Sep 17 00:00:00 2001 From: stemangiola Date: Mon, 15 Nov 2021 16:45:24 +1100 Subject: [PATCH 01/10] first implementation --- DESCRIPTION | 2 +- R/functions.R | 5 +++-- R/methods.R | 29 +++++++++++++++++------------ R/utilities.R | 5 +++-- man/add_annotation.Rd | 1 + man/add_bar-method.Rd | 4 ++-- man/add_line-method.Rd | 4 ++-- man/add_point-method.Rd | 4 ++-- man/add_tile-method.Rd | 4 ++-- tests/testthat/tests.R | 17 +++++++++++++++++ vignettes/introduction.Rmd | 9 ++++++++- 11 files changed, 58 insertions(+), 26 deletions(-) diff --git a/DESCRIPTION b/DESCRIPTION index 91820b9..6eeeb17 100644 --- a/DESCRIPTION +++ b/DESCRIPTION @@ -64,5 +64,5 @@ Biarch: true biocViews: AssayDomain, Infrastructure Encoding: UTF-8 LazyData: true -RoxygenNote: 7.1.1 +RoxygenNote: 7.1.2 Language: en-US diff --git a/R/functions.R b/R/functions.R index 583ac02..f95a696 100644 --- a/R/functions.R +++ b/R/functions.R @@ -280,6 +280,7 @@ add_grouping = function(my_input_heatmap){ #' @importFrom rlang is_function #' @importFrom purrr when #' @importFrom rlang dots_list +#' @importFrom grid unit #' #' @name add_annotation #' @rdname add_annotation @@ -304,7 +305,7 @@ add_annotation = function(my_input_heatmap, annotation, type = rep("tile", length(quo_names(annotation))), palette_discrete = list(), - palette_continuous = list(), ...) { + palette_continuous = list(), size = unit(2, "cm"), ...) { # Solve CRAN note annot_type = NULL @@ -364,7 +365,7 @@ add_annotation = function(my_input_heatmap, get_top_left_annotation( !!.horizontal, !!.vertical, !!.abundance, - !!annotation, palette_annotation, type, x_y_annot_cols, ...) + !!annotation, palette_annotation, type, x_y_annot_cols, size, ...) # Number of grouping how_many_discrete = .data_annot %>% filter(annot_type=="discrete") %>% nrow diff --git a/R/methods.R b/R/methods.R index 165f4b6..7055a98 100644 --- a/R/methods.R +++ b/R/methods.R @@ -312,7 +312,7 @@ setMethod("heatmap", "tbl_df", heatmap_) #' #' @importFrom rlang enquo #' @importFrom magrittr "%>%" -#' +#' @importFrom grid unit #' #' @name add_tile #' @rdname add_tile-method @@ -346,7 +346,7 @@ setMethod("heatmap", "tbl_df", heatmap_) #' @export setGeneric("add_tile", function(.data, .column, - palette = NULL, ...) + palette = NULL, size = unit(2, "cm"), ...) standardGeneric("add_tile")) #' add_tile @@ -358,7 +358,7 @@ setGeneric("add_tile", function(.data, #' setMethod("add_tile", "InputHeatmap", function(.data, .column, - palette = NULL, ...){ + palette = NULL, size = unit(2, "cm"),...){ .column = enquo(.column) @@ -381,6 +381,8 @@ setMethod("add_tile", "InputHeatmap", function(.data, select(!!.column) %>% sapply(class) %>% when(. %in% c("integer", "numerical", "numeric", "double") & !is.null(palette) ~ list(palette), ~ list()), + + size = size, ... ) @@ -394,6 +396,7 @@ setMethod("add_tile", "InputHeatmap", function(.data, #' #' @importFrom rlang enquo #' @importFrom magrittr "%>%" +#' @importFrom grid unit #' #' #' @name add_point @@ -428,7 +431,7 @@ setMethod("add_tile", "InputHeatmap", function(.data, #' @export setGeneric("add_point", function(.data, .column, - palette = NULL, ...) + palette = NULL, size = unit(2, "cm"),...) standardGeneric("add_point")) #' add_point @@ -440,11 +443,11 @@ setGeneric("add_point", function(.data, #' setMethod("add_point", "InputHeatmap", function(.data, .column, - palette = NULL, ...){ + palette = NULL, size = unit(2, "cm"),...){ .column = enquo(.column) - .data %>% add_annotation( !!.column, type = "point", ...) + .data %>% add_annotation( !!.column, type = "point", size = size,...) }) @@ -456,6 +459,7 @@ setMethod("add_point", "InputHeatmap", function(.data, #' #' @importFrom rlang enquo #' @importFrom magrittr "%>%" +#' @importFrom grid unit #' #' #' @name add_line @@ -490,7 +494,7 @@ setMethod("add_point", "InputHeatmap", function(.data, #' @export setGeneric("add_line", function(.data, .column, - palette = NULL, ...) + palette = NULL,size = unit(2, "cm"), ...) standardGeneric("add_line")) #' add_line @@ -503,11 +507,11 @@ setGeneric("add_line", function(.data, #' setMethod("add_line", "InputHeatmap", function(.data, .column, - palette = NULL, ...){ + palette = NULL, size = unit(2, "cm"),...){ .column = enquo(.column) - .data %>% add_annotation( !!.column, type = "line", ...) + .data %>% add_annotation( !!.column, type = "line", size = size,...) }) @@ -519,6 +523,7 @@ setMethod("add_line", "InputHeatmap", function(.data, #' #' @importFrom rlang enquo #' @importFrom magrittr "%>%" +#' @importFrom grid unit #' #' #' @name add_bar @@ -553,7 +558,7 @@ setMethod("add_line", "InputHeatmap", function(.data, #' @export setGeneric("add_bar", function(.data, .column, - palette = NULL, ...) + palette = NULL, size = unit(2, "cm"),...) standardGeneric("add_bar")) #' add_bar @@ -565,11 +570,11 @@ setGeneric("add_bar", function(.data, #' setMethod("add_bar", "InputHeatmap", function(.data, .column, - palette = NULL, ...){ + palette = NULL, size = unit(2, "cm"),...){ .column = enquo(.column) - .data %>% add_annotation( !!.column, type = "bar", ...) + .data %>% add_annotation( !!.column, type = "bar", size = size,...) }) diff --git a/R/utilities.R b/R/utilities.R index a8d5a03..5417501 100644 --- a/R/utilities.R +++ b/R/utilities.R @@ -624,7 +624,7 @@ type_to_annot_function = list( "line" = anno_lines ) -get_top_left_annotation = function(.data_, .column, .row, .abundance, annotation, palette_annotation, type, x_y_annot_cols, ...){ +get_top_left_annotation = function(.data_, .column, .row, .abundance, annotation, palette_annotation, type, x_y_annot_cols, size, ...){ # Comply with CRAN NOTES data = NULL @@ -685,7 +685,8 @@ get_top_left_annotation = function(.data_, .column, .row, .abundance, annotation fx = ..2 # Do conditional - if(is_function(fx)) fx(..1, which=..3) + if(is_function(fx) & ..3 == "column") fx(..1, which=..3, height = size) + if(is_function(fx) & ..3 == "row") fx(..1, which=..3, width = size) else .x })) %>% diff --git a/man/add_annotation.Rd b/man/add_annotation.Rd index bd213f3..0377361 100644 --- a/man/add_annotation.Rd +++ b/man/add_annotation.Rd @@ -10,6 +10,7 @@ add_annotation( type = rep("tile", length(quo_names(annotation))), palette_discrete = list(), palette_continuous = list(), + size = unit(2, "cm"), ... ) } diff --git a/man/add_bar-method.Rd b/man/add_bar-method.Rd index 72e17d0..87be1be 100644 --- a/man/add_bar-method.Rd +++ b/man/add_bar-method.Rd @@ -6,9 +6,9 @@ \alias{add_bar,InputHeatmap-method} \title{Adds a bar annotation layer to a `InputHeatmap`, that on evaluation creates a `ComplexHeatmap`} \usage{ -add_bar(.data, .column, palette = NULL, ...) +add_bar(.data, .column, palette = NULL, size = unit(2, "cm"), ...) -\S4method{add_bar}{InputHeatmap}(.data, .column, palette = NULL, ...) +\S4method{add_bar}{InputHeatmap}(.data, .column, palette = NULL, size = unit(2, "cm"), ...) } \arguments{ \item{.data}{A `tbl_df` formatted as | | | | <...> |} diff --git a/man/add_line-method.Rd b/man/add_line-method.Rd index 5625d2a..a3f7658 100644 --- a/man/add_line-method.Rd +++ b/man/add_line-method.Rd @@ -6,9 +6,9 @@ \alias{add_line,InputHeatmap-method} \title{Adds a line annotation layer to a `InputHeatmap`, that on evaluation creates a `ComplexHeatmap`} \usage{ -add_line(.data, .column, palette = NULL, ...) +add_line(.data, .column, palette = NULL, size = unit(2, "cm"), ...) -\S4method{add_line}{InputHeatmap}(.data, .column, palette = NULL, ...) +\S4method{add_line}{InputHeatmap}(.data, .column, palette = NULL, size = unit(2, "cm"), ...) } \arguments{ \item{.data}{A `tbl_df` formatted as | | | | <...> |} diff --git a/man/add_point-method.Rd b/man/add_point-method.Rd index 6d0e0c1..105fd08 100644 --- a/man/add_point-method.Rd +++ b/man/add_point-method.Rd @@ -6,9 +6,9 @@ \alias{add_point,InputHeatmap-method} \title{Adds a point annotation layer to a `InputHeatmap`, that on evaluation creates a `ComplexHeatmap`} \usage{ -add_point(.data, .column, palette = NULL, ...) +add_point(.data, .column, palette = NULL, size = unit(2, "cm"), ...) -\S4method{add_point}{InputHeatmap}(.data, .column, palette = NULL, ...) +\S4method{add_point}{InputHeatmap}(.data, .column, palette = NULL, size = unit(2, "cm"), ...) } \arguments{ \item{.data}{A `tbl_df` formatted as | | | | <...> |} diff --git a/man/add_tile-method.Rd b/man/add_tile-method.Rd index b8fb24b..a8e997f 100644 --- a/man/add_tile-method.Rd +++ b/man/add_tile-method.Rd @@ -6,9 +6,9 @@ \alias{add_tile,InputHeatmap-method} \title{Adds a tile annotation layer to a `InputHeatmap`, that on evaluation creates a `ComplexHeatmap`} \usage{ -add_tile(.data, .column, palette = NULL, ...) +add_tile(.data, .column, palette = NULL, size = unit(2, "cm"), ...) -\S4method{add_tile}{InputHeatmap}(.data, .column, palette = NULL, ...) +\S4method{add_tile}{InputHeatmap}(.data, .column, palette = NULL, size = unit(2, "cm"), ...) } \arguments{ \item{.data}{A `tbl_df` formatted as | | | | <...> |} diff --git a/tests/testthat/tests.R b/tests/testthat/tests.R index 8d95c64..cb921fa 100644 --- a/tests/testthat/tests.R +++ b/tests/testthat/tests.R @@ -575,3 +575,20 @@ test_that("legend",{ }) +test_that("size annotation",{ + + p = + tidyHeatmap::N52 |> + tidyHeatmap::heatmap( + .row = symbol_ct, + .column = UBR, + .value = `read count normalised log`, + show_heatmap_legend = FALSE + ) |> + add_tile(UBR, size = unit(10, "mm")) |> + add_tile(`Cell type`, size = unit(10, "mm")) + + vdiffr::expect_doppelganger("legend", p) + + +}) \ No newline at end of file diff --git a/vignettes/introduction.Rmd b/vignettes/introduction.Rmd index 0d757cc..e6166ff 100644 --- a/vignettes/introduction.Rmd +++ b/vignettes/introduction.Rmd @@ -3,7 +3,9 @@ title: "Overview of the tidyHeatmap package" author: "Stefano Mangiola" date: "`r Sys.Date()`" package: tidyHeatmap -output: rmarkdown::html_vignette +output: + html_vignette: + toc_float: true vignette: > %\VignetteIndexEntry{Overview of the tidyHeatmap package} %\VignetteEngine{knitr::rmarkdown} @@ -18,3 +20,8 @@ vignette: > ```{r child="man/fragments/intro.Rmd"} ``` +# Session Info + +```{r} +sessionInfo() +``` \ No newline at end of file From c9b41d60a72e7a9fefd2c602747e8099b53a08de Mon Sep 17 00:00:00 2001 From: stemangiola Date: Mon, 15 Nov 2021 17:43:56 +1100 Subject: [PATCH 02/10] fixed some mechanics --- R/utilities.R | 44 ++++++++++++++++++++++-------------------- tests/testthat/tests.R | 4 ++-- 2 files changed, 25 insertions(+), 23 deletions(-) diff --git a/R/utilities.R b/R/utilities.R index 5417501..8142181 100644 --- a/R/utilities.R +++ b/R/utilities.R @@ -618,7 +618,7 @@ ct_colors = function(ct) #' @importFrom ComplexHeatmap anno_barplot #' @importFrom ComplexHeatmap anno_lines type_to_annot_function = list( - "tile" = NULL, + "tile" = anno_simple, "point" = anno_points, "bar" = anno_barplot, "line" = anno_lines @@ -668,7 +668,7 @@ get_top_left_annotation = function(.data_, .column, .row, .abundance, annotation .data_ %>% ungroup() %>% select(.y, .x) %>% - distinct() %>% + distinct() %>% arrange_at(vars(.y)) %>% pull(.x) ) @@ -677,17 +677,33 @@ get_top_left_annotation = function(.data_, .column, .row, .abundance, annotation # Add function mutate(fx = annotation_function) %>% + # Add color indexes separately for each orientation + mutate(annot_type = map_chr(data, ~ .x %>% when(class(.) %in% c("factor", "character", "logical") ~ "discrete", + class(.) %in% c("integer", "numerical", "numeric", "double") ~ "continuous", + ~ "other" + ) )) %>% + group_by(annot_type) %>% + mutate(idx = row_number()) %>% + ungroup() %>% + mutate(color = map2(data, idx, ~ { + if(.x %>% class %in% c("factor", "character", "logical")) + colorRampPalette(palette_annotation$discrete[[.y]])(length(unique(.x))) %>% setNames(unique(.x)) + else if (.x %>% class %in% c("integer", "numerical", "numeric", "double")) + colorRampPalette(palette_annotation$continuous[[.y]])(length(.x)) %>% colorRamp2(seq(min(.x), max(.x), length.out = length(.x)), .) + else NULL + })) %>% + # Apply annot function if not NULL otherwise pass original annotation # This because no function for ComplexHeatmap = to tile - mutate(annot = pmap(list(data, fx, orientation), ~ { + mutate(annot = pmap(list(data, fx, orientation, color = color), ~ { # Trick needed for map BUG: could not find function "..2" fx = ..2 # Do conditional - if(is_function(fx) & ..3 == "column") fx(..1, which=..3, height = size) - if(is_function(fx) & ..3 == "row") fx(..1, which=..3, width = size) - else .x + if(..3 == "column") fx(..1, which=..3, height = size, col=..4) + else if(..3 == "row") fx(..1, which=..3, width = size, col=..4) + else stop("tidyHeatmap says: this should not happen. In the internal function get_top_left_annotation") })) %>% # # Check if NA in annotations @@ -696,21 +712,7 @@ get_top_left_annotation = function(.data_, .column, .row, .abundance, annotation # else { x } # } ) %>% - # Add color indexes separately for each orientation - mutate(annot_type = map_chr(annot, ~ .x %>% when(class(.) %in% c("factor", "character", "logical") ~ "discrete", - class(.) %in% c("integer", "numerical", "numeric", "double") ~ "continuous", - ~ "other" - ) )) %>% - group_by(annot_type) %>% - mutate(idx = row_number()) %>% - ungroup() %>% - mutate(color = map2(annot, idx, ~ { - if(.x %>% class %in% c("factor", "character", "logical")) - colorRampPalette(palette_annotation$discrete[[.y]])(length(unique(.x))) %>% setNames(unique(.x)) - else if (.x %>% class %in% c("integer", "numerical", "numeric", "double")) - colorRampPalette(palette_annotation$continuous[[.y]])(length(.x)) %>% colorRamp2(seq(min(.x), max(.x), length.out = length(.x)), .) - else NULL - })) %>% + mutate(further_arguments = map(col_name, ~ dots_args)) %>% diff --git a/tests/testthat/tests.R b/tests/testthat/tests.R index cb921fa..43c3e21 100644 --- a/tests/testthat/tests.R +++ b/tests/testthat/tests.R @@ -585,8 +585,8 @@ test_that("size annotation",{ .value = `read count normalised log`, show_heatmap_legend = FALSE ) |> - add_tile(UBR, size = unit(10, "mm")) |> - add_tile(`Cell type`, size = unit(10, "mm")) + add_tile(UBR, size = unit(20, "mm")) |> + add_tile(`Cell type`, size = unit(2, "mm")) vdiffr::expect_doppelganger("legend", p) From 20493e2b1d72b3f5767f3db1919b67b28db70428 Mon Sep 17 00:00:00 2001 From: stemangiola Date: Mon, 15 Nov 2021 19:18:21 +1100 Subject: [PATCH 03/10] fixed setup --- R/functions.R | 2 +- R/methods.R | 16 +++++------ R/utilities.R | 64 +++++++++++++++++++++++++++++++++++------ man/add_bar-method.Rd | 4 +-- man/add_line-method.Rd | 4 +-- man/add_point-method.Rd | 4 +-- man/add_tile-method.Rd | 4 +-- 7 files changed, 72 insertions(+), 26 deletions(-) diff --git a/R/functions.R b/R/functions.R index f95a696..68f9ba6 100644 --- a/R/functions.R +++ b/R/functions.R @@ -305,7 +305,7 @@ add_annotation = function(my_input_heatmap, annotation, type = rep("tile", length(quo_names(annotation))), palette_discrete = list(), - palette_continuous = list(), size = unit(2, "cm"), ...) { + palette_continuous = list(), size = NULL, ...) { # Solve CRAN note annot_type = NULL diff --git a/R/methods.R b/R/methods.R index 7055a98..b20c86b 100644 --- a/R/methods.R +++ b/R/methods.R @@ -346,7 +346,7 @@ setMethod("heatmap", "tbl_df", heatmap_) #' @export setGeneric("add_tile", function(.data, .column, - palette = NULL, size = unit(2, "cm"), ...) + palette = NULL, size = NULL, ...) standardGeneric("add_tile")) #' add_tile @@ -358,7 +358,7 @@ setGeneric("add_tile", function(.data, #' setMethod("add_tile", "InputHeatmap", function(.data, .column, - palette = NULL, size = unit(2, "cm"),...){ + palette = NULL, size = NULL,...){ .column = enquo(.column) @@ -431,7 +431,7 @@ setMethod("add_tile", "InputHeatmap", function(.data, #' @export setGeneric("add_point", function(.data, .column, - palette = NULL, size = unit(2, "cm"),...) + palette = NULL, size = NULL,...) standardGeneric("add_point")) #' add_point @@ -443,7 +443,7 @@ setGeneric("add_point", function(.data, #' setMethod("add_point", "InputHeatmap", function(.data, .column, - palette = NULL, size = unit(2, "cm"),...){ + palette = NULL, size = NULL,...){ .column = enquo(.column) @@ -494,7 +494,7 @@ setMethod("add_point", "InputHeatmap", function(.data, #' @export setGeneric("add_line", function(.data, .column, - palette = NULL,size = unit(2, "cm"), ...) + palette = NULL,size = NULL, ...) standardGeneric("add_line")) #' add_line @@ -507,7 +507,7 @@ setGeneric("add_line", function(.data, #' setMethod("add_line", "InputHeatmap", function(.data, .column, - palette = NULL, size = unit(2, "cm"),...){ + palette = NULL, size = NULL,...){ .column = enquo(.column) @@ -558,7 +558,7 @@ setMethod("add_line", "InputHeatmap", function(.data, #' @export setGeneric("add_bar", function(.data, .column, - palette = NULL, size = unit(2, "cm"),...) + palette = NULL, size = NULL,...) standardGeneric("add_bar")) #' add_bar @@ -570,7 +570,7 @@ setGeneric("add_bar", function(.data, #' setMethod("add_bar", "InputHeatmap", function(.data, .column, - palette = NULL, size = unit(2, "cm"),...){ + palette = NULL, size = NULL,...){ .column = enquo(.column) diff --git a/R/utilities.R b/R/utilities.R index 8142181..148338e 100644 --- a/R/utilities.R +++ b/R/utilities.R @@ -618,7 +618,7 @@ ct_colors = function(ct) #' @importFrom ComplexHeatmap anno_barplot #' @importFrom ComplexHeatmap anno_lines type_to_annot_function = list( - "tile" = anno_simple, + "tile" = NULL, #anno_simple, "point" = anno_points, "bar" = anno_barplot, "line" = anno_lines @@ -701,9 +701,9 @@ get_top_left_annotation = function(.data_, .column, .row, .abundance, annotation fx = ..2 # Do conditional - if(..3 == "column") fx(..1, which=..3, height = size, col=..4) - else if(..3 == "row") fx(..1, which=..3, width = size, col=..4) - else stop("tidyHeatmap says: this should not happen. In the internal function get_top_left_annotation") + if(is_function(fx) & ..3 == "column") fx(..1, which=..3, height = size) + else if(is_function(fx) & ..3 == "row") fx(..1, which=..3, width = size) + else .x # else stop("tidyHeatmap says: this should not happen. In the internal function get_top_left_annotation") })) %>% # # Check if NA in annotations @@ -712,9 +712,14 @@ get_top_left_annotation = function(.data_, .column, .row, .abundance, annotation # else { x } # } ) %>% - - - mutate(further_arguments = map(col_name, ~ dots_args)) %>% + mutate(further_arguments = map2( + col_name, fx, + ~ dots_args %>% + + # If tile add size as further argument + when(!is_function(.y) ~ c(., list(simple_anno_size = size)), ~ (.)) + + )) %>% # Stop if annotations discrete bigger than palette @@ -1121,8 +1126,49 @@ combine_elements_with_the_same_name = function(x){ if(length(unlist(x))==0) return(unlist(x)) else { - x = unlist(x) - tapply(unlist(x, use.names = FALSE), rep(names(x), lengths(x)), FUN = c) + list_df = + map_dfr(x, ~ enframe(.x)) %>% + mutate(my_class = map_chr(value, ~class(.x)[[1]])) + + # The current backend does not allow multiple tails sizes + if( + list_df %>% + filter(my_class == "simpleUnit") %>% + nrow() %>% + gt(1) && + list_df %>% + filter(my_class == "simpleUnit") %>% + pull(value) %>% + reduce(identical) %>% + not() + ) + warning("tidyHeatmap says: the current backend only allows for one tail annotation size. The latter one will be selected.") + + # Select one size + list_df = + bind_rows( + list_df %>% + filter(my_class == "simpleUnit") %>% + tail(1), + list_df %>% + filter(my_class != "simpleUnit") + ) %>% + nest(data = -c(name, my_class)) %>% + mutate(vector = map2( + data, my_class, + ~ { + if(.y == "simpleUnit") reduce(.x$value, unit.c) + else reduce(.x$value, c) + } + )) + + + list_df %>% + pull(vector) %>% + setNames(list_df$name) + + # x = unlist(x) + # tapply(unlist(x, use.names = FALSE), rep(names(x), lengths(x)), FUN = c) } } diff --git a/man/add_bar-method.Rd b/man/add_bar-method.Rd index 87be1be..e5e39b4 100644 --- a/man/add_bar-method.Rd +++ b/man/add_bar-method.Rd @@ -6,9 +6,9 @@ \alias{add_bar,InputHeatmap-method} \title{Adds a bar annotation layer to a `InputHeatmap`, that on evaluation creates a `ComplexHeatmap`} \usage{ -add_bar(.data, .column, palette = NULL, size = unit(2, "cm"), ...) +add_bar(.data, .column, palette = NULL, size = NULL, ...) -\S4method{add_bar}{InputHeatmap}(.data, .column, palette = NULL, size = unit(2, "cm"), ...) +\S4method{add_bar}{InputHeatmap}(.data, .column, palette = NULL, size = NULL, ...) } \arguments{ \item{.data}{A `tbl_df` formatted as | | | | <...> |} diff --git a/man/add_line-method.Rd b/man/add_line-method.Rd index a3f7658..ab5f766 100644 --- a/man/add_line-method.Rd +++ b/man/add_line-method.Rd @@ -6,9 +6,9 @@ \alias{add_line,InputHeatmap-method} \title{Adds a line annotation layer to a `InputHeatmap`, that on evaluation creates a `ComplexHeatmap`} \usage{ -add_line(.data, .column, palette = NULL, size = unit(2, "cm"), ...) +add_line(.data, .column, palette = NULL, size = NULL, ...) -\S4method{add_line}{InputHeatmap}(.data, .column, palette = NULL, size = unit(2, "cm"), ...) +\S4method{add_line}{InputHeatmap}(.data, .column, palette = NULL, size = NULL, ...) } \arguments{ \item{.data}{A `tbl_df` formatted as | | | | <...> |} diff --git a/man/add_point-method.Rd b/man/add_point-method.Rd index 105fd08..c66c7ea 100644 --- a/man/add_point-method.Rd +++ b/man/add_point-method.Rd @@ -6,9 +6,9 @@ \alias{add_point,InputHeatmap-method} \title{Adds a point annotation layer to a `InputHeatmap`, that on evaluation creates a `ComplexHeatmap`} \usage{ -add_point(.data, .column, palette = NULL, size = unit(2, "cm"), ...) +add_point(.data, .column, palette = NULL, size = NULL, ...) -\S4method{add_point}{InputHeatmap}(.data, .column, palette = NULL, size = unit(2, "cm"), ...) +\S4method{add_point}{InputHeatmap}(.data, .column, palette = NULL, size = NULL, ...) } \arguments{ \item{.data}{A `tbl_df` formatted as | | | | <...> |} diff --git a/man/add_tile-method.Rd b/man/add_tile-method.Rd index a8e997f..d0f69c6 100644 --- a/man/add_tile-method.Rd +++ b/man/add_tile-method.Rd @@ -6,9 +6,9 @@ \alias{add_tile,InputHeatmap-method} \title{Adds a tile annotation layer to a `InputHeatmap`, that on evaluation creates a `ComplexHeatmap`} \usage{ -add_tile(.data, .column, palette = NULL, size = unit(2, "cm"), ...) +add_tile(.data, .column, palette = NULL, size = NULL, ...) -\S4method{add_tile}{InputHeatmap}(.data, .column, palette = NULL, size = unit(2, "cm"), ...) +\S4method{add_tile}{InputHeatmap}(.data, .column, palette = NULL, size = NULL, ...) } \arguments{ \item{.data}{A `tbl_df` formatted as | | | | <...> |} From 077746b16569d6f64dde3b62adfcb7086d364934 Mon Sep 17 00:00:00 2001 From: stemangiola Date: Mon, 15 Nov 2021 19:22:29 +1100 Subject: [PATCH 04/10] built docs --- R/functions.R | 1 + R/methods.R | 4 ++++ man/add_annotation.Rd | 4 +++- man/add_bar-method.Rd | 2 ++ man/add_line-method.Rd | 2 ++ man/add_point-method.Rd | 2 ++ man/add_tile-method.Rd | 2 ++ 7 files changed, 16 insertions(+), 1 deletion(-) diff --git a/R/functions.R b/R/functions.R index 68f9ba6..9bfe75e 100644 --- a/R/functions.R +++ b/R/functions.R @@ -290,6 +290,7 @@ add_grouping = function(my_input_heatmap){ #' @param type A character vector of the set c(\"tile\", \"point\", \"bar\", \"line\") #' @param palette_discrete A list of character vectors. This is the list of palettes that will be used for horizontal and vertical discrete annotations. The discrete classification of annotations depends on the column type of your input tibble (e.g., character and factor). #' @param palette_continuous A list of character vectors. This is the list of palettes that will be used for horizontal and vertical continuous annotations. The continuous classification of annotations depends on the column type of your input tibble (e.g., integer, numerical, double). +#' @param size A grid::unit object, e.g. unit(2, "cm"). This is the height or width of the annotation depending on the orientation. #' @param ... The arguments that will be passed to top_annotation or left_annotation of the ComplexHeatmap container #' #' @details To be added. diff --git a/R/methods.R b/R/methods.R index b20c86b..88bb0a1 100644 --- a/R/methods.R +++ b/R/methods.R @@ -320,6 +320,7 @@ setMethod("heatmap", "tbl_df", heatmap_) #' @param .data A `tbl_df` formatted as | | | | <...> | #' @param .column Vector of quotes #' @param palette A character vector of colors This is the list of palettes that will be used for horizontal and vertical discrete annotations. The discrete classification of annotations depends on the column type of your input tibble (e.g., character and factor). +#' @param size A grid::unit object, e.g. unit(2, "cm"). This is the height or width of the annotation depending on the orientation. #' @param ... The arguments that will be passed to top_annotation or left_annotation of the ComplexHeatmap container #' #' @details It uses `ComplexHeatmap` as visualisation tool. @@ -405,6 +406,7 @@ setMethod("add_tile", "InputHeatmap", function(.data, #' @param .data A `tbl_df` formatted as | | | | <...> | #' @param .column Vector of quotes #' @param palette A character vector of colors This is the list of palettes that will be used for horizontal and vertical discrete annotations. The discrete classification of annotations depends on the column type of your input tibble (e.g., character and factor). +#' @param size A grid::unit object, e.g. unit(2, "cm"). This is the height or width of the annotation depending on the orientation. #' @param ... The arguments that will be passed to top_annotation or left_annotation of the ComplexHeatmap container #' #' @details It uses `ComplexHeatmap` as visualisation tool. @@ -468,6 +470,7 @@ setMethod("add_point", "InputHeatmap", function(.data, #' @param .data A `tbl_df` formatted as | | | | <...> | #' @param .column Vector of quotes #' @param palette A character vector of colors This is the list of palettes that will be used for horizontal and vertical discrete annotations. The discrete classification of annotations depends on the column type of your input tibble (e.g., character and factor). +#' @param size A grid::unit object, e.g. unit(2, "cm"). This is the height or width of the annotation depending on the orientation. #' @param ... The arguments that will be passed to top_annotation or left_annotation of the ComplexHeatmap container #' #' @details It uses `ComplexHeatmap` as visualisation tool. @@ -532,6 +535,7 @@ setMethod("add_line", "InputHeatmap", function(.data, #' @param .data A `tbl_df` formatted as | | | | <...> | #' @param .column Vector of quotes #' @param palette A character vector of colors This is the list of palettes that will be used for horizontal and vertical discrete annotations. The discrete classification of annotations depends on the column type of your input tibble (e.g., character and factor). +#' @param size A grid::unit object, e.g. unit(2, "cm"). This is the height or width of the annotation depending on the orientation. #' @param ... The arguments that will be passed to top_annotation or left_annotation of the ComplexHeatmap container #' #' @details It uses `ComplexHeatmap` as visualisation tool. diff --git a/man/add_annotation.Rd b/man/add_annotation.Rd index 0377361..d75fc71 100644 --- a/man/add_annotation.Rd +++ b/man/add_annotation.Rd @@ -10,7 +10,7 @@ add_annotation( type = rep("tile", length(quo_names(annotation))), palette_discrete = list(), palette_continuous = list(), - size = unit(2, "cm"), + size = NULL, ... ) } @@ -25,6 +25,8 @@ add_annotation( \item{palette_continuous}{A list of character vectors. This is the list of palettes that will be used for horizontal and vertical continuous annotations. The continuous classification of annotations depends on the column type of your input tibble (e.g., integer, numerical, double).} +\item{size}{A grid::unit object, e.g. unit(2, "cm"). This is the height or width of the annotation depending on the orientation.} + \item{...}{The arguments that will be passed to top_annotation or left_annotation of the ComplexHeatmap container} } \value{ diff --git a/man/add_bar-method.Rd b/man/add_bar-method.Rd index e5e39b4..97efd66 100644 --- a/man/add_bar-method.Rd +++ b/man/add_bar-method.Rd @@ -17,6 +17,8 @@ add_bar(.data, .column, palette = NULL, size = NULL, ...) \item{palette}{A character vector of colors This is the list of palettes that will be used for horizontal and vertical discrete annotations. The discrete classification of annotations depends on the column type of your input tibble (e.g., character and factor).} +\item{size}{A grid::unit object, e.g. unit(2, "cm"). This is the height or width of the annotation depending on the orientation.} + \item{...}{The arguments that will be passed to top_annotation or left_annotation of the ComplexHeatmap container} } \value{ diff --git a/man/add_line-method.Rd b/man/add_line-method.Rd index ab5f766..778020d 100644 --- a/man/add_line-method.Rd +++ b/man/add_line-method.Rd @@ -17,6 +17,8 @@ add_line(.data, .column, palette = NULL, size = NULL, ...) \item{palette}{A character vector of colors This is the list of palettes that will be used for horizontal and vertical discrete annotations. The discrete classification of annotations depends on the column type of your input tibble (e.g., character and factor).} +\item{size}{A grid::unit object, e.g. unit(2, "cm"). This is the height or width of the annotation depending on the orientation.} + \item{...}{The arguments that will be passed to top_annotation or left_annotation of the ComplexHeatmap container} } \value{ diff --git a/man/add_point-method.Rd b/man/add_point-method.Rd index c66c7ea..9753273 100644 --- a/man/add_point-method.Rd +++ b/man/add_point-method.Rd @@ -17,6 +17,8 @@ add_point(.data, .column, palette = NULL, size = NULL, ...) \item{palette}{A character vector of colors This is the list of palettes that will be used for horizontal and vertical discrete annotations. The discrete classification of annotations depends on the column type of your input tibble (e.g., character and factor).} +\item{size}{A grid::unit object, e.g. unit(2, "cm"). This is the height or width of the annotation depending on the orientation.} + \item{...}{The arguments that will be passed to top_annotation or left_annotation of the ComplexHeatmap container} } \value{ diff --git a/man/add_tile-method.Rd b/man/add_tile-method.Rd index d0f69c6..ac4591e 100644 --- a/man/add_tile-method.Rd +++ b/man/add_tile-method.Rd @@ -17,6 +17,8 @@ add_tile(.data, .column, palette = NULL, size = NULL, ...) \item{palette}{A character vector of colors This is the list of palettes that will be used for horizontal and vertical discrete annotations. The discrete classification of annotations depends on the column type of your input tibble (e.g., character and factor).} +\item{size}{A grid::unit object, e.g. unit(2, "cm"). This is the height or width of the annotation depending on the orientation.} + \item{...}{The arguments that will be passed to top_annotation or left_annotation of the ComplexHeatmap container} } \value{ From ef993c048f998f6710755cde85a81aa0fb7d7d38 Mon Sep 17 00:00:00 2001 From: stemangiola Date: Mon, 15 Nov 2021 19:44:51 +1100 Subject: [PATCH 05/10] also parse correctly the gpars --- R/utilities.R | 13 ++++++++++++- 1 file changed, 12 insertions(+), 1 deletion(-) diff --git a/R/utilities.R b/R/utilities.R index 148338e..184421f 100644 --- a/R/utilities.R +++ b/R/utilities.R @@ -1145,7 +1145,7 @@ combine_elements_with_the_same_name = function(x){ warning("tidyHeatmap says: the current backend only allows for one tail annotation size. The latter one will be selected.") # Select one size - list_df = + list_df = bind_rows( list_df %>% filter(my_class == "simpleUnit") %>% @@ -1158,6 +1158,7 @@ combine_elements_with_the_same_name = function(x){ data, my_class, ~ { if(.y == "simpleUnit") reduce(.x$value, unit.c) + else if(.y == "gpar") combine_lists_with_the_same_name(.x$value) %>% as.list() %>% do.call(gpar, .) else reduce(.x$value, c) } )) @@ -1172,3 +1173,13 @@ combine_elements_with_the_same_name = function(x){ } } + +combine_lists_with_the_same_name = function(x){ + + if(length(unlist(x))==0) return(unlist(x)) + else { + x = unlist(x) + tapply(unlist(x, use.names = FALSE), rep(names(x), lengths(x)), FUN = c) + } + +} From ed44d3c674459ce73b8c6fd396784a461621add6 Mon Sep 17 00:00:00 2001 From: stemangiola Date: Fri, 19 Nov 2021 09:12:41 +1100 Subject: [PATCH 06/10] add annotation size to README --- NAMESPACE | 3 + R/utilities.R | 4 + man/fragments/intro.Rmd | 24 +- man/fragments/intro.html | 559 +++++++++++++++++++++++++++++++++++++++ 4 files changed, 587 insertions(+), 3 deletions(-) create mode 100644 man/fragments/intro.html diff --git a/NAMESPACE b/NAMESPACE index ab284e5..50496fc 100644 --- a/NAMESPACE +++ b/NAMESPACE @@ -40,9 +40,11 @@ importFrom(purrr,as_mapper) importFrom(purrr,map) importFrom(purrr,map2) importFrom(purrr,map_chr) +importFrom(purrr,map_dfr) importFrom(purrr,map_lgl) importFrom(purrr,negate) importFrom(purrr,pmap) +importFrom(purrr,reduce) importFrom(purrr,when) importFrom(rlang,dots_list) importFrom(rlang,enquo) @@ -58,6 +60,7 @@ importFrom(stats,hclust) importFrom(stats,sd) importFrom(stats,setNames) importFrom(stats,terms) +importFrom(tibble,enframe) importFrom(tibble,rowid_to_column) importFrom(utils,capture.output) importFrom(utils,head) diff --git a/R/utilities.R b/R/utilities.R index 184421f..8049142 100644 --- a/R/utilities.R +++ b/R/utilities.R @@ -743,6 +743,7 @@ get_top_left_annotation = function(.data_, .column, .row, .abundance, annotation } +#' @importFrom grid unit get_group_annotation = function(.data, .column, .row, .abundance, palette_annotation){ # Comply with CRAN NOTES @@ -1122,6 +1123,9 @@ not = function(is){ !is } # Raise to the power pow = function(a,b){ a^b } +#' @importFrom purrr map_dfr +#' @importFrom purrr reduce +#' @importFrom tibble enframe combine_elements_with_the_same_name = function(x){ if(length(unlist(x))==0) return(unlist(x)) diff --git a/man/fragments/intro.Rmd b/man/fragments/intro.Rmd index 9fba451..690fa41 100644 --- a/man/fragments/intro.Rmd +++ b/man/fragments/intro.Rmd @@ -78,6 +78,7 @@ Let's transform the mtcars dataset into a tidy "element-feature-independent vari library(dplyr) library(tidyr) library(tidyHeatmap) +library(grid) ``` ```{r} @@ -219,6 +220,7 @@ tidyHeatmap::pasilla |> add_tile(condition) |> add_tile(activation) ``` + Remove legends, adding aesthetics to annotations in a modular fashion, using `ComplexHeatmap` arguments ```{r} @@ -237,9 +239,7 @@ tidyHeatmap::pasilla |> ## Annotation types -**This feature requires >= 0.99.20 version** - -"tile" (default), "point", "bar" and "line" are available +"tile", "point", "bar" and "line" are available ```{r} # Create some more data points @@ -265,6 +265,24 @@ pasilla_plus |> add_line(age) ``` +## Annotation size + +We can customise annotation sizes, using the `grid::unit()` + +```{r} +pasilla_plus |> + heatmap( + .column = sample, + .row = symbol, + .value = `count normalised adjusted` + ) |> + add_tile(condition, size = unit(0.3, "cm")) |> + add_point(activation, size = unit(0.3, "cm")) |> + add_tile(act, size = unit(0.3, "cm")) |> + add_bar(size, size = unit(0.3, "cm")) |> + add_line(age, size = unit(0.3, "cm")) +``` + # Layer symbol Add a layer on top of the heatmap diff --git a/man/fragments/intro.html b/man/fragments/intro.html new file mode 100644 index 0000000..d5a1527 --- /dev/null +++ b/man/fragments/intro.html @@ -0,0 +1,559 @@ + + + + + + + + + + + + + +intro.knit + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
+ + + + + + + +
+

Citation

+

Mangiola et al., (2020). tidyHeatmap: an R package for modular heatmap production based on tidy principles. Journal of Open Source Software, 5(52), 2472, https://doi.org/10.21105/joss.02472

+

Please have a look also to

+ +

website: stemangiola.github.io/tidyHeatmap

+

tidyHeatmap is a package that introduces tidy principles to the creation of information-rich heatmaps. This package uses ComplexHeatmap as graphical engine.

+

Advantages:

+
    +
  • Modular annotation with just specifying column names
    • +
  • Custom grouping of rows is easy to specify providing a grouped tbl. For example df |> group_by(...)
    • +
  • Labels size adjusted by row and column total number
    • +
  • Default use of Brewer and Viridis palettes
    • +
+
+
+

Functions/utilities available

+ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
FunctionDescription
heatmapPlots base heatmap
add_tileAdds tile annotation to the heatmap
add_pointAdds point annotation to the heatmap
add_barAdds bar annotation to the heatmap
add_lineAdds line annotation to the heatmap
layer_pointAdds layer of symbols on top of the heatmap
layer_squareAdds layer of symbols on top of the heatmap
layer_diamondAdds layer of symbols on top of the heatmap
layer_arrow_upAdds layer of symbols on top of the heatmap
layer_arrow_downAdd layer of symbols on top of the heatmap
split_rowsSplits the rows based on the dendogram
split_columnsSplits the columns based on the dendogram
save_pdfSaves the PDF of the heatmap
+
+
+

Installation

+

To install the most up-to-date version

+
devtools::install_github("stemangiola/tidyHeatmap")
+

To install the most stable version (however please keep in mind that this package is under a maturing lifecycle stage)

+
install.packages("tidyHeatmap")
+
+
+

Contribution

+

If you want to contribute to the software, report issues or problems with the software or seek support please open an issue here

+
+
+

Input data frame

+

The heatmaps visualise a multi-element, multi-feature dataset, annotated with independent variables. Each observation is a element-feature pair (e.g., person-physical characteristics).

+ + + + + + + + + + + + + + + + + +
elementfeaturevalueindependent_variables
chr or fctrchr or fctrnumeric…
+

Let’s transform the mtcars dataset into a tidy “element-feature-independent variables” data frame. Where the independent variables in this case are ‘hp’ and ‘vs’.

+
mtcars_tidy <- 
+    mtcars |> 
+    as_tibble(rownames="Car name") |> 
+    
+    # Scale
+    mutate_at(vars(-`Car name`, -hp, -vs), scale) |>
+    
+    # tidyfy
+    pivot_longer(cols = -c(`Car name`, hp, vs), names_to = "Property", values_to = "Value")
+
+mtcars_tidy
+
## # A tibble: 288 x 5
+##    `Car name`       hp    vs Property Value[,1]
+##    <chr>         <dbl> <dbl> <chr>        <dbl>
+##  1 Mazda RX4       110     0 mpg          0.151
+##  2 Mazda RX4       110     0 cyl         -0.105
+##  3 Mazda RX4       110     0 disp        -0.571
+##  4 Mazda RX4       110     0 drat         0.568
+##  5 Mazda RX4       110     0 wt          -0.610
+##  6 Mazda RX4       110     0 qsec        -0.777
+##  7 Mazda RX4       110     0 am           1.19 
+##  8 Mazda RX4       110     0 gear         0.424
+##  9 Mazda RX4       110     0 carb         0.735
+## 10 Mazda RX4 Wag   110     0 mpg          0.151
+## # … with 278 more rows
+
+
+

Plotting

+

For plotting, you simply pipe the input data frame into heatmap, specifying:

+
    +
  • The rows, cols relative column names (mandatory)
    • +
  • The value column name (mandatory)
    • +
  • The annotations column name(s)
    • +
+

mtcars

+
mtcars_heatmap <- 
+    mtcars_tidy |> 
+    heatmap(`Car name`, Property, Value ) |>
+    add_tile(hp)
+
## tidyHeatmap says: (once per session) from release 1.2.3 the grouping labels have white background by default. To add color for one-ay grouping specify palette_grouping = list(c("red", "blue"))
+
mtcars_heatmap
+

+
+
+

Saving

+
mtcars_heatmap |> save_pdf("mtcars_heatmap.pdf")
+
+
+

Grouping and splitting

+

We can easily group the data (one group per dimension maximum, at the moment only the vertical dimension is supported) with dplyr, and the heatmap will be grouped accordingly

+
mtcars_tidy |> 
+    group_by(vs) |>
+    heatmap(`Car name`, Property, Value ) |>
+    add_tile(hp)
+

+

We can provide colour palettes to groupings

+
mtcars_tidy |> 
+    group_by(vs) |>
+    heatmap(
+        `Car name`, Property, Value ,
+        palette_grouping = list(c("#66C2A5", "#FC8D62"))
+    ) |>
+    add_tile(hp)
+

+

We can split based on the cladogram

+
mtcars_tidy |> 
+    heatmap(`Car name`, Property, Value ) |>
+    split_rows(2) |>
+    split_columns(2)
+

+

We can Split on kmean clustering (using ComplexHeatmap options, it is stokastic)

+
mtcars_tidy |> 
+    heatmap(
+        `Car name`, Property, Value ,
+        row_km = 2,
+        column_km = 2
+    )
+

+
+
+

Custom palettes

+

We can easily use custom palette, using strings, hexadecimal color character vector,

+
mtcars_tidy |> 
+    heatmap(
+        `Car name`, 
+        Property, 
+        Value,
+        palette_value = c("red", "white", "blue")
+    )
+

+

A better-looking blue-to-red palette

+
mtcars_tidy |> 
+    heatmap(
+        `Car name`, 
+        Property, 
+        Value,
+        palette_value = circlize::colorRamp2(
+            seq(-2, 2, length.out = 11), 
+            RColorBrewer::brewer.pal(11, "RdBu")
+        )
+    )
+

+

Or a grid::colorRamp2 function for higher flexibility

+
mtcars_tidy |> 
+    heatmap(
+        `Car name`, 
+        Property, 
+        Value,
+        palette_value = circlize::colorRamp2(c(-2, -1, 0, 1, 2), viridis::magma(5))
+    )
+

+
+
+

Multiple groupings and annotations

+
tidyHeatmap::pasilla |>
+    group_by(location, type) |>
+    heatmap(
+        .column = sample,
+        .row = symbol,
+        .value = `count normalised adjusted`
+    ) |>
+    add_tile(condition) |>
+    add_tile(activation)
+

+

Remove legends, adding aesthetics to annotations in a modular fashion, using ComplexHeatmap arguments

+
tidyHeatmap::pasilla |>
+    group_by(location, type) |>
+    heatmap(
+        .column = sample,
+        .row = symbol,
+        .value = `count normalised adjusted`,
+        show_heatmap_legend = FALSE
+    ) |>
+    add_tile(condition, show_legend = FALSE) |>
+    add_tile(activation, show_legend = FALSE)
+

+
+
+

Annotation types

+

“tile”, “point”, “bar” and “line” are available

+
# Create some more data points
+pasilla_plus <- 
+    tidyHeatmap::pasilla |>
+    dplyr::mutate(act = activation) |> 
+    tidyr::nest(data = -sample) |>
+    dplyr::mutate(size = rnorm(n(), 4,0.5)) |>
+    dplyr::mutate(age = runif(n(), 50, 200)) |>
+    tidyr::unnest(data) 
+
+# Plot
+pasilla_plus |>
+    heatmap(
+        .column = sample,
+        .row = symbol,
+        .value = `count normalised adjusted`
+    ) |>
+    add_tile(condition) |>
+    add_point(activation) |>
+    add_tile(act) |>
+    add_bar(size) |>
+    add_line(age)
+

+
+
+

Annotation size

+

We can customise annotation sizes, using the grid::unit()

+
pasilla_plus |>
+    heatmap(
+        .column = sample,
+        .row = symbol,
+        .value = `count normalised adjusted`
+    ) |>
+    add_tile(condition, size = unit(0.3, "cm")) |>
+    add_point(activation, size = unit(0.3, "cm")) |>
+    add_tile(act, size = unit(0.3, "cm")) |>
+    add_bar(size, size = unit(0.3, "cm")) |>
+    add_line(age, size = unit(0.3, "cm"))
+

+
+
+

Layer symbol

+

Add a layer on top of the heatmap

+
tidyHeatmap::pasilla |>
+    
+    # filter
+    filter(symbol %in% head(unique(tidyHeatmap::pasilla$symbol), n = 10)) |>
+    
+    heatmap(
+        .column = sample,
+        .row = symbol,
+        .value = `count normalised adjusted`
+    ) |> 
+    layer_point(
+        `count normalised adjusted log` > 6 & sample == "untreated3" 
+    )
+

+
+
+

ComplexHeatmap further styling

+

Add cell borders

+
mtcars_tidy |> 
+    heatmap(
+        `Car name`, Property, Value, 
+        rect_gp = grid::gpar(col = "#161616", lwd = 0.5)
+    )
+

+

Drop row clustering

+
mtcars_tidy |> 
+    heatmap(
+        `Car name`, Property, Value, 
+        cluster_rows = FALSE
+    )
+

+

Reorder rows elements

+
library(forcats)
+mtcars_tidy |> 
+    mutate(`Car name` = fct_reorder(`Car name`, `Car name`, .desc = TRUE)) %>% 
+    heatmap(
+        `Car name`, Property, Value, 
+        cluster_rows = FALSE
+    )
+

+
+ + + + +
+ + + + + + + + + + + + + + + From 62e4eea09df0fc92e6ba20983c68cdbf307d61f4 Mon Sep 17 00:00:00 2001 From: stemangiola Date: Fri, 19 Nov 2021 09:26:45 +1100 Subject: [PATCH 07/10] update README with styling --- README.md | 76 +++++++++++++++---- man/fragments/figures/unnamed-chunk-18-1.png | Bin 48223 -> 48052 bytes man/fragments/figures/unnamed-chunk-19-1.png | Bin 24627 -> 45798 bytes man/fragments/figures/unnamed-chunk-20-1.png | Bin 52746 -> 24627 bytes man/fragments/figures/unnamed-chunk-21-1.png | Bin 42202 -> 52746 bytes man/fragments/figures/unnamed-chunk-22-1.png | Bin 41956 -> 42202 bytes man/fragments/figures/unnamed-chunk-23-1.png | Bin 0 -> 41956 bytes man/fragments/figures/unnamed-chunk-24-1.png | Bin 0 -> 45713 bytes man/fragments/figures/unnamed-chunk-25-1.png | Bin 0 -> 34858 bytes man/fragments/intro.Rmd | 42 +++++++--- 10 files changed, 96 insertions(+), 22 deletions(-) create mode 100644 man/fragments/figures/unnamed-chunk-23-1.png create mode 100644 man/fragments/figures/unnamed-chunk-24-1.png create mode 100644 man/fragments/figures/unnamed-chunk-25-1.png diff --git a/README.md b/README.md index 7cdb00f..bcdf151 100644 --- a/README.md +++ b/README.md @@ -272,9 +272,10 @@ tidyHeatmap::pasilla |> add_tile(activation) ``` -![](man/fragments/figures/unnamed-chunk-16-1.png) Remove -legends, adding aesthetics to annotations in a modular fashion, using -`ComplexHeatmap` arguments +![](man/fragments/figures/unnamed-chunk-16-1.png) + +Remove legends, adding aesthetics to annotations in a modular fashion, +using `ComplexHeatmap` arguments ``` r tidyHeatmap::pasilla |> @@ -293,9 +294,7 @@ tidyHeatmap::pasilla |> ## Annotation types -**This feature requires \>= 0.99.20 version** - -“tile” (default), “point”, “bar” and “line” are available +“tile”, “point”, “bar” and “line” are available ``` r # Create some more data points @@ -323,6 +322,27 @@ pasilla_plus |> ![](man/fragments/figures/unnamed-chunk-18-1.png) +## Annotation size + +We can customise annotation sizes using the `grid::unit()`, and the size +of their names using in-built `ComplexHeatmap` arguments + +``` r +pasilla_plus |> + heatmap( + .column = sample, + .row = symbol, + .value = `count normalised adjusted` + ) |> + add_tile(condition, size = unit(0.3, "cm"), annotation_name_gp= gpar(fontsize = 8)) |> + add_point(activation, size = unit(0.3, "cm"), annotation_name_gp= gpar(fontsize = 8)) |> + add_tile(act, size = unit(0.3, "cm"), annotation_name_gp= gpar(fontsize = 8)) |> + add_bar(size, size = unit(0.3, "cm"), annotation_name_gp= gpar(fontsize = 8)) |> + add_line(age, size = unit(0.3, "cm"), annotation_name_gp= gpar(fontsize = 8)) +``` + +![](man/fragments/figures/unnamed-chunk-19-1.png) + # Layer symbol Add a layer on top of the heatmap @@ -343,11 +363,11 @@ tidyHeatmap::pasilla |> ) ``` -![](man/fragments/figures/unnamed-chunk-19-1.png) +![](man/fragments/figures/unnamed-chunk-20-1.png) # ComplexHeatmap further styling -Add cell borders +## Add cell borders ``` r mtcars_tidy |> @@ -357,9 +377,9 @@ mtcars_tidy |> ) ``` -![](man/fragments/figures/unnamed-chunk-20-1.png) +![](man/fragments/figures/unnamed-chunk-21-1.png) -Drop row clustering +## Drop row clustering ``` r mtcars_tidy |> @@ -369,9 +389,9 @@ mtcars_tidy |> ) ``` -![](man/fragments/figures/unnamed-chunk-21-1.png) +![](man/fragments/figures/unnamed-chunk-22-1.png) -Reorder rows elements +## Reorder rows elements ``` r library(forcats) @@ -383,4 +403,34 @@ mtcars_tidy |> ) ``` -![](man/fragments/figures/unnamed-chunk-22-1.png) +![](man/fragments/figures/unnamed-chunk-23-1.png) + +## Size of dendrograms + +``` r +mtcars_tidy |> + mutate(`Car name` = fct_reorder(`Car name`, `Car name`, .desc = TRUE)) %>% + heatmap( + `Car name`, Property, Value, + column_dend_height = unit(0.2, "cm"), + row_dend_width = unit(0.2, "cm") + ) +``` + +![](man/fragments/figures/unnamed-chunk-24-1.png) + +## Size of rows/columns titles and names + +``` r +mtcars_tidy |> + mutate(`Car name` = 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zCzDH^Wi)8keBd|L7Yq^o1-71ye3Rb=9XiA?hAU2B<_EkeKrK<_jzN7*LbkgM*-y?| zatexqE74nk!!UO{B#KJhq{Y8_PeB6A0Xg|DD<&zajjvP%MJwN_oybR%;hf3ebyIsK qWBmU~r&fy8cmCP`+h1q5No1vz?MPjB@e*QSVW!F4>Cwh3JpT{1uw}yl literal 0 HcmV?d00001 diff --git a/man/fragments/intro.Rmd b/man/fragments/intro.Rmd index 690fa41..27ded36 100644 --- a/man/fragments/intro.Rmd +++ b/man/fragments/intro.Rmd @@ -267,7 +267,7 @@ pasilla_plus |> ## Annotation size -We can customise annotation sizes, using the `grid::unit()` +We can customise annotation sizes using the `grid::unit()`, and the size of their names using in-built `ComplexHeatmap` arguments ```{r} pasilla_plus |> @@ -276,11 +276,11 @@ pasilla_plus |> .row = symbol, .value = `count normalised adjusted` ) |> - add_tile(condition, size = unit(0.3, "cm")) |> - add_point(activation, size = unit(0.3, "cm")) |> - add_tile(act, size = unit(0.3, "cm")) |> - add_bar(size, size = unit(0.3, "cm")) |> - add_line(age, size = unit(0.3, "cm")) + add_tile(condition, size = unit(0.3, "cm"), annotation_name_gp= gpar(fontsize = 8)) |> + add_point(activation, size = unit(0.3, "cm"), annotation_name_gp= gpar(fontsize = 8)) |> + add_tile(act, size = unit(0.3, "cm"), annotation_name_gp= gpar(fontsize = 8)) |> + add_bar(size, size = unit(0.3, "cm"), annotation_name_gp= gpar(fontsize = 8)) |> + add_line(age, size = unit(0.3, "cm"), annotation_name_gp= gpar(fontsize = 8)) ``` # Layer symbol @@ -305,7 +305,7 @@ tidyHeatmap::pasilla |> # ComplexHeatmap further styling -Add cell borders +## Add cell borders ```{r} mtcars_tidy |> @@ -315,7 +315,7 @@ mtcars_tidy |> ) ``` -Drop row clustering +## Drop row clustering ```{r} mtcars_tidy |> @@ -325,7 +325,7 @@ mtcars_tidy |> ) ``` -Reorder rows elements +## Reorder rows elements ```{r} library(forcats) @@ -337,5 +337,29 @@ mtcars_tidy |> ) ``` +## Size of dendrograms +```{r} +mtcars_tidy |> + mutate(`Car name` = fct_reorder(`Car name`, `Car name`, .desc = TRUE)) %>% + heatmap( + `Car name`, Property, Value, + column_dend_height = unit(0.2, "cm"), + row_dend_width = unit(0.2, "cm") + ) +``` + +## Size of rows/columns titles and names + +```{r} +mtcars_tidy |> + mutate(`Car name` = fct_reorder(`Car name`, `Car name`, .desc = TRUE)) %>% + heatmap( + `Car name`, Property, Value, + row_names_gp = gpar(fontsize = 7), + column_names_gp = gpar(fontsize = 7), + column_title_gp = gpar(fontsize = 7), + row_title_gp = gpar(fontsize = 7) + ) +``` From b4172e44f6d1897131cc9f95f8e8232b4ea2837f Mon Sep 17 00:00:00 2001 From: stemangiola Date: Fri, 19 Nov 2021 10:39:59 +1100 Subject: [PATCH 08/10] fix colors for non tiles --- NAMESPACE | 1 + R/utilities.R | 183 ++-- .../testthat/_snaps/tests/size-annotation.svg | 981 ++++++++++++++++++ tests/testthat/tests.R | 4 +- 4 files changed, 1077 insertions(+), 92 deletions(-) create mode 100644 tests/testthat/_snaps/tests/size-annotation.svg diff --git a/NAMESPACE b/NAMESPACE index 50496fc..5274185 100644 --- a/NAMESPACE +++ b/NAMESPACE @@ -28,6 +28,7 @@ importFrom(grDevices,colorRampPalette) importFrom(grid,gpar) importFrom(grid,grid.points) importFrom(grid,unit) +importFrom(grid,unit.c) importFrom(lifecycle,deprecate_warn) importFrom(lifecycle,is_present) importFrom(magrittr,"%$%") diff --git a/R/utilities.R b/R/utilities.R index 8049142..cbed49e 100644 --- a/R/utilities.R +++ b/R/utilities.R @@ -649,96 +649,98 @@ get_top_left_annotation = function(.data_, .column, .row, .abundance, annotation # Create dataset quo_names(annotation) %>% - as_tibble %>% - rename(col_name = value) %>% - - # delete if annotation is NULL - when(quo_is_null(annotation) ~ slice(., 0), ~ (.)) %>% - - # Add orientation - left_join(x_y_annot_cols, by = "col_name") %>% - mutate(col_orientation = map_chr(orientation, ~ .x %>% when((.) == "column" ~ quo_name(.column), (.) == "row" ~ quo_name(.row)))) %>% - - # Add data - mutate( - data = map2( - col_name, - col_orientation, - ~ - .data_ %>% - ungroup() %>% - select(.y, .x) %>% - distinct() %>% - arrange_at(vars(.y)) %>% - pull(.x) - ) - ) %>% - - # Add function - mutate(fx = annotation_function) %>% - - # Add color indexes separately for each orientation - mutate(annot_type = map_chr(data, ~ .x %>% when(class(.) %in% c("factor", "character", "logical") ~ "discrete", - class(.) %in% c("integer", "numerical", "numeric", "double") ~ "continuous", - ~ "other" - ) )) %>% - group_by(annot_type) %>% - mutate(idx = row_number()) %>% - ungroup() %>% - mutate(color = map2(data, idx, ~ { - if(.x %>% class %in% c("factor", "character", "logical")) - colorRampPalette(palette_annotation$discrete[[.y]])(length(unique(.x))) %>% setNames(unique(.x)) - else if (.x %>% class %in% c("integer", "numerical", "numeric", "double")) - colorRampPalette(palette_annotation$continuous[[.y]])(length(.x)) %>% colorRamp2(seq(min(.x), max(.x), length.out = length(.x)), .) - else NULL - })) %>% - - # Apply annot function if not NULL otherwise pass original annotation - # This because no function for ComplexHeatmap = to tile - mutate(annot = pmap(list(data, fx, orientation, color = color), ~ { - - # Trick needed for map BUG: could not find function "..2" - fx = ..2 - - # Do conditional - if(is_function(fx) & ..3 == "column") fx(..1, which=..3, height = size) - else if(is_function(fx) & ..3 == "row") fx(..1, which=..3, width = size) - else .x # else stop("tidyHeatmap says: this should not happen. In the internal function get_top_left_annotation") - })) %>% - - # # Check if NA in annotations - # mutate_at(vars(!!annotation), function(x) { - # if(any(is.na(x))) { warning("tidyHeatmap says: You have NAs into your annotation column"); replace_na(x, "NA"); } - # else { x } - # } ) %>% - - mutate(further_arguments = map2( - col_name, fx, - ~ dots_args %>% - - # If tile add size as further argument - when(!is_function(.y) ~ c(., list(simple_anno_size = size)), ~ (.)) - - )) %>% - - - # Stop if annotations discrete bigger than palette - when( - (.) %>% pull(data) %>% map_chr(~ .x %>% class) %in% - c("factor", "character") %>% which %>% length %>% - gt(palette_annotation$discrete %>% length) ~ - stop("tidyHeatmap says: Your discrete annotaton columns are bigger than the palette available"), - ~ (.) - ) %>% - - # Stop if annotations continuous bigger than palette - when( - (.) %>% pull(data) %>% map_chr(~ .x %>% class) %in% - c("int", "dbl", "numeric") %>% which %>% length %>% - gt( palette_annotation$continuous %>% length) ~ - stop("tidyHeatmap says: Your continuous annotaton columns are bigger than the palette available"), - ~ (.) - ) + as_tibble %>% + rename(col_name = value) %>% + + # delete if annotation is NULL + when(quo_is_null(annotation) ~ slice(., 0), ~ (.)) %>% + + # Add orientation + left_join(x_y_annot_cols, by = "col_name") %>% + mutate(col_orientation = map_chr(orientation, ~ .x %>% when((.) == "column" ~ quo_name(.column), (.) == "row" ~ quo_name(.row)))) %>% + + # Add data + mutate( + data = map2( + col_name, + col_orientation, + ~ + .data_ %>% + ungroup() %>% + select(.y, .x) %>% + distinct() %>% + arrange_at(vars(.y)) %>% + pull(.x) + ) + ) %>% + + # Add function + mutate(fx = annotation_function) %>% + + + + # Apply annot function if not NULL otherwise pass original annotation + # This because no function for ComplexHeatmap = to tile + mutate(annot = pmap(list(data, fx, orientation), ~ { + + # Trick needed for map BUG: could not find function "..2" + fx = ..2 + + # Do conditional + if(is_function(fx) & ..3 == "column") fx(..1, which=..3, height = size) + else if(is_function(fx) & ..3 == "row") fx(..1, which=..3, width = size) + else .x # else stop("tidyHeatmap says: this should not happen. In the internal function get_top_left_annotation") + })) %>% + + # # Check if NA in annotations + # mutate_at(vars(!!annotation), function(x) { + # if(any(is.na(x))) { warning("tidyHeatmap says: You have NAs into your annotation column"); replace_na(x, "NA"); } + # else { x } + # } ) %>% + + # Add color indexes separately for each orientation + mutate(annot_type = map_chr(annot, ~ .x %>% when(class(.) %in% c("factor", "character", "logical") ~ "discrete", + class(.) %in% c("integer", "numerical", "numeric", "double") ~ "continuous", + ~ "other" + ) )) %>% + group_by(annot_type) %>% + mutate(idx = row_number()) %>% + ungroup() %>% + mutate(color = map2(annot, idx, ~ { + if(.x %>% class %in% c("factor", "character", "logical")) + colorRampPalette(palette_annotation$discrete[[.y]])(length(unique(.x))) %>% setNames(unique(.x)) + else if (.x %>% class %in% c("integer", "numerical", "numeric", "double")) + colorRampPalette(palette_annotation$continuous[[.y]])(length(.x)) %>% colorRamp2(seq(min(.x), max(.x), length.out = length(.x)), .) + else NULL + })) %>% + + mutate(further_arguments = map2( + col_name, fx, + ~ dots_args %>% + + # If tile add size as further argument + when(!is_function(.y) ~ c(., list(simple_anno_size = size)), ~ (.)) + + )) %>% + + + # Stop if annotations discrete bigger than palette + when( + (.) %>% pull(data) %>% map_chr(~ .x %>% class) %in% + c("factor", "character") %>% which %>% length %>% + gt(palette_annotation$discrete %>% length) ~ + stop("tidyHeatmap says: Your discrete annotaton columns are bigger than the palette available"), + ~ (.) + ) %>% + + # Stop if annotations continuous bigger than palette + when( + (.) %>% pull(data) %>% map_chr(~ .x %>% class) %in% + c("int", "dbl", "numeric") %>% which %>% length %>% + gt( palette_annotation$continuous %>% length) ~ + stop("tidyHeatmap says: Your continuous annotaton columns are bigger than the palette available"), + ~ (.) + ) } @@ -1126,6 +1128,7 @@ pow = function(a,b){ a^b } #' @importFrom purrr map_dfr #' @importFrom purrr reduce #' @importFrom tibble enframe +#' @importFrom grid unit.c combine_elements_with_the_same_name = function(x){ if(length(unlist(x))==0) return(unlist(x)) diff --git a/tests/testthat/_snaps/tests/size-annotation.svg b/tests/testthat/_snaps/tests/size-annotation.svg new file mode 100644 index 0000000..8d563ac --- /dev/null +++ b/tests/testthat/_snaps/tests/size-annotation.svg @@ -0,0 +1,981 @@ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +UBR +symbol_ct + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +M_CHPT1 +F_C5 +E_EPHA6 +E_DCN +T_LIF +T_NOTCH3 +T_PDGFD +F_CXCL14 +M_CD47 +F_VWF +M_ANGPT2 +M_VWF +T_PRG4 +E_CPA3 +M_MDK +F_GPER1 +M_PLOD3 +M_SERPINE1 +E_CHI3L1 +F_ADAMTS13 +E_COL3A1 +M_HEG1 +M_CAV1 +T_PROK2 +F_CXCL10 +M_ADAM12 +M_ITGA4 +M_ITGB8 +E_ORM1 +F_C1QA +F_C1QB +M_FN1 +M_SEMA3E +M_MEGF8 +M_PRKD1 +E_SPINK5 +T_HYAL1 +T_MFAP4 +M_SEMA6A +F_FGFR2 +11420 +11405 +11438 +11437 +11452 +11444 +11433 +11425 +11427 +11453 +11458 +11450 +11467 + + + + + + + + + + + + + +UBR + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +Cell type +Cell type +E +F +M +T + + + + +UBR +11400 +11420 +11440 +11460 +11480 + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + diff --git a/tests/testthat/tests.R b/tests/testthat/tests.R index 43c3e21..fbedd99 100644 --- a/tests/testthat/tests.R +++ b/tests/testthat/tests.R @@ -586,9 +586,9 @@ test_that("size annotation",{ show_heatmap_legend = FALSE ) |> add_tile(UBR, size = unit(20, "mm")) |> - add_tile(`Cell type`, size = unit(2, "mm")) + add_tile(`Cell type`, size = unit(20, "mm")) - vdiffr::expect_doppelganger("legend", p) + vdiffr::expect_doppelganger("size annotation", p) }) \ No newline at end of file From 4557b217c5f39b5b9b0110660aabdc4bbb628dad Mon Sep 17 00:00:00 2001 From: stemangiola Date: Fri, 19 Nov 2021 10:58:51 +1100 Subject: [PATCH 09/10] fix CHECKs --- DESCRIPTION | 3 ++- R/utilities.R | 9 ++++++++- vignettes/introduction.Rmd | 2 +- 3 files changed, 11 insertions(+), 3 deletions(-) diff --git a/DESCRIPTION b/DESCRIPTION index 6eeeb17..715d9a6 100644 --- a/DESCRIPTION +++ b/DESCRIPTION @@ -55,7 +55,8 @@ Suggests: rmarkdown, qpdf, covr, - roxygen2 + roxygen2, + forcats VignetteBuilder: knitr RdMacros: diff --git a/R/utilities.R b/R/utilities.R index cbed49e..a83da3c 100644 --- a/R/utilities.R +++ b/R/utilities.R @@ -723,7 +723,6 @@ get_top_left_annotation = function(.data_, .column, .row, .abundance, annotation )) %>% - # Stop if annotations discrete bigger than palette when( (.) %>% pull(data) %>% map_chr(~ .x %>% class) %in% @@ -1077,6 +1076,11 @@ annot_to_list = function(.data){ # Comply with CRAN NOTES col_name = NULL annot = NULL + value = NULL + my_cells = NULL + name = NULL + data = NULL + .data %>% pull(annot) %>% @@ -1131,6 +1135,9 @@ pow = function(a,b){ a^b } #' @importFrom grid unit.c combine_elements_with_the_same_name = function(x){ + # Fix CRAN notes + my_class = NULL + if(length(unlist(x))==0) return(unlist(x)) else { list_df = diff --git a/vignettes/introduction.Rmd b/vignettes/introduction.Rmd index e6166ff..0cffd4f 100644 --- a/vignettes/introduction.Rmd +++ b/vignettes/introduction.Rmd @@ -17,7 +17,7 @@ vignette: > [![DOI](https://joss.theoj.org/papers/10.21105/joss.02472/status.svg)](https://doi.org/10.21105/joss.02472) -```{r child="man/fragments/intro.Rmd"} +```{r child="../man/fragments/intro.Rmd"} ``` # Session Info From 010174e9c790fde0ab6d65b4f0ed596e98f58222 Mon Sep 17 00:00:00 2001 From: Stefano Mangiola Date: Fri, 19 Nov 2021 11:00:31 +1100 Subject: [PATCH 10/10] Update functions.R --- R/functions.R | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/R/functions.R b/R/functions.R index 9bfe75e..72580b3 100644 --- a/R/functions.R +++ b/R/functions.R @@ -36,7 +36,7 @@ #' @param transform A function, used to transform .value, for example log1p #' @param .scale A character string. Possible values are c(\"none\", \"row\", \"column\", \"both\") #' @param palette_value A character vector, or a function for higher customisation (colorRamp2). This is the palette that will be used as gradient for abundance. If palette_value is a vector of hexadecimal colours, it should have 3 values. If you want more customisation, you can pass to palette_value a function, that is derived as for example `colorRamp2(c(-2, 0, 2), palette_value)` -#' @param palette_grouping A list of character vectors. This is the list of palettes that will be used for grouping +#' @param palette_grouping A list of character vectors. This is the list of palettes that will be used for grouping #' @param ... Further arguments to be passed to ComplexHeatmap::Heatmap #' #' @details To be added.