diff --git a/CHANGELOG.md b/CHANGELOG.md
index c6be95ffc9a1..e8ffcf6c0414 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -7,6 +7,10 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ## [Unreleased]
 
+### Added
+
+- Support `keyframes` in JS config file themes ([14594](https://github.com/tailwindlabs/tailwindcss/pull/14594))
+
 ### Fixed
 
 - Don’t crash when scanning a candidate equal to the configured prefix ([#14588](https://github.com/tailwindlabs/tailwindcss/pull/14588))
diff --git a/packages/tailwindcss/src/compat/apply-compat-hooks.ts b/packages/tailwindcss/src/compat/apply-compat-hooks.ts
index f702e5202292..1de9281f9a1d 100644
--- a/packages/tailwindcss/src/compat/apply-compat-hooks.ts
+++ b/packages/tailwindcss/src/compat/apply-compat-hooks.ts
@@ -5,6 +5,7 @@ import { withAlpha } from '../utilities'
 import { segment } from '../utils/segment'
 import { toKeyPath } from '../utils/to-key-path'
 import { applyConfigToTheme } from './apply-config-to-theme'
+import { applyKeyframesToAst } from './apply-keyframes-to-ast'
 import { createCompatConfig } from './config/create-compat-config'
 import { resolveConfig } from './config/resolve-config'
 import type { UserConfig } from './config/types'
@@ -206,6 +207,7 @@ export async function applyCompatibilityHooks({
   // config would otherwise expand into namespaces like `background-color` which
   // core utilities already read from.
   applyConfigToTheme(designSystem, resolvedUserConfig)
+  applyKeyframesToAst(ast, resolvedUserConfig)
 
   registerThemeVariantOverrides(resolvedUserConfig, designSystem)
   registerScreensConfig(resolvedUserConfig, designSystem)
diff --git a/packages/tailwindcss/src/compat/apply-keyframes-to-ast.test.ts b/packages/tailwindcss/src/compat/apply-keyframes-to-ast.test.ts
new file mode 100644
index 000000000000..dd95e0b3bfc5
--- /dev/null
+++ b/packages/tailwindcss/src/compat/apply-keyframes-to-ast.test.ts
@@ -0,0 +1,54 @@
+import { expect, test } from 'vitest'
+import { toCss, type AstNode } from '../ast'
+import { buildDesignSystem } from '../design-system'
+import { Theme } from '../theme'
+import { applyKeyframesToAst } from './apply-keyframes-to-ast'
+import { resolveConfig } from './config/resolve-config'
+
+test('Config values can be merged into the theme', () => {
+  let theme = new Theme()
+  let design = buildDesignSystem(theme)
+
+  let ast: AstNode[] = []
+
+  let resolvedUserConfig = resolveConfig(design, [
+    {
+      config: {
+        theme: {
+          keyframes: {
+            'fade-in': {
+              '0%': { opacity: '0' },
+              '100%': { opacity: '1' },
+            },
+            'fade-out': {
+              '0%': { opacity: '1' },
+              '100%': { opacity: '0' },
+            },
+          },
+        },
+      },
+      base: '/root',
+    },
+  ])
+  applyKeyframesToAst(ast, resolvedUserConfig)
+
+  expect(toCss(ast)).toMatchInlineSnapshot(`
+    "@keyframes fade-in {
+      0% {
+        opacity: 0;
+      }
+      100% {
+        opacity: 1;
+      }
+    }
+    @keyframes fade-out {
+      0% {
+        opacity: 1;
+      }
+      100% {
+        opacity: 0;
+      }
+    }
+    "
+  `)
+})
diff --git a/packages/tailwindcss/src/compat/apply-keyframes-to-ast.ts b/packages/tailwindcss/src/compat/apply-keyframes-to-ast.ts
new file mode 100644
index 000000000000..2ca12ab50256
--- /dev/null
+++ b/packages/tailwindcss/src/compat/apply-keyframes-to-ast.ts
@@ -0,0 +1,11 @@
+import { rule, type AstNode } from '../ast'
+import type { ResolvedConfig } from './config/types'
+import { objectToAst } from './plugin-api'
+
+export function applyKeyframesToAst(ast: AstNode[], { theme }: ResolvedConfig) {
+  if ('keyframes' in theme) {
+    for (let [name, keyframe] of Object.entries(theme.keyframes)) {
+      ast.push(rule(`@keyframes ${name}`, objectToAst(keyframe as any)))
+    }
+  }
+}
diff --git a/packages/tailwindcss/src/compat/plugin-api.test.ts b/packages/tailwindcss/src/compat/plugin-api.test.ts
index cbf4b29ece79..c0e04d576b09 100644
--- a/packages/tailwindcss/src/compat/plugin-api.test.ts
+++ b/packages/tailwindcss/src/compat/plugin-api.test.ts
@@ -67,6 +67,18 @@ describe('theme', async () => {
           }
         }
       }
+      @keyframes enter {
+        from {
+          opacity: var(--tw-enter-opacity, 1);
+          transform: translate3d(var(--tw-enter-translate-x, 0), var(--tw-enter-translate-y, 0), 0) scale3d(var(--tw-enter-scale, 1), var(--tw-enter-scale, 1), var(--tw-enter-scale, 1)) rotate(var(--tw-enter-rotate, 0));
+        }
+      }
+      @keyframes exit {
+        to {
+          opacity: var(--tw-exit-opacity, 1);
+          transform: translate3d(var(--tw-exit-translate-x, 0), var(--tw-exit-translate-y, 0), 0) scale3d(var(--tw-exit-scale, 1), var(--tw-exit-scale, 1), var(--tw-exit-scale, 1)) rotate(var(--tw-exit-rotate, 0));
+        }
+      }
       "
     `)
   })
diff --git a/packages/tailwindcss/src/compat/plugin-api.ts b/packages/tailwindcss/src/compat/plugin-api.ts
index f6d8d91a57e1..c4d28de9a9e3 100644
--- a/packages/tailwindcss/src/compat/plugin-api.ts
+++ b/packages/tailwindcss/src/compat/plugin-api.ts
@@ -384,7 +384,7 @@ export function buildPluginApi(
 
 export type CssInJs = { [key: string]: string | string[] | CssInJs | CssInJs[] }
 
-function objectToAst(rules: CssInJs | CssInJs[]): AstNode[] {
+export function objectToAst(rules: CssInJs | CssInJs[]): AstNode[] {
   let ast: AstNode[] = []
 
   rules = Array.isArray(rules) ? rules : [rules]