diff --git a/.changeset/four-buttons-run.md b/.changeset/four-buttons-run.md
new file mode 100644
index 0000000000..e89c4f7d8d
--- /dev/null
+++ b/.changeset/four-buttons-run.md
@@ -0,0 +1,5 @@
+---
+"trigger.dev": patch
+---
+
+Ignore OTEL_EXPORTER_OTLP_ENDPOINT environment variable from `.env` files, to prevent the internal OTEL_EXPORTER_OTLP_ENDPOINT being overwritten with a user-supplied value.
diff --git a/packages/cli-v3/src/utilities/dotEnv.ts b/packages/cli-v3/src/utilities/dotEnv.ts
index 717aa88c59..7b5bfbcf6d 100644
--- a/packages/cli-v3/src/utilities/dotEnv.ts
+++ b/packages/cli-v3/src/utilities/dotEnv.ts
@@ -21,6 +21,7 @@ export function resolveDotEnvVars(cwd?: string, envFile?: string) {
   // remove TRIGGER_API_URL and TRIGGER_SECRET_KEY, since those should be coming from the worker
   delete result.TRIGGER_API_URL;
   delete result.TRIGGER_SECRET_KEY;
+  delete result.OTEL_EXPORTER_OTLP_ENDPOINT;
 
   return result;
 }