diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml
index 05bec3386..cfc073586 100644
--- a/.github/workflows/ci.yml
+++ b/.github/workflows/ci.yml
@@ -48,7 +48,7 @@ jobs:
     strategy:
       fail-fast: false
       matrix:
-        os: [ ubuntu-latest ]
+        os: [ ubuntu-latest, macos-latest ]
     runs-on: ${{ matrix.os }}
     if: ${{ !startsWith(github.event.head_commit.message, 'release:') && !startsWith(github.event.head_commit.message, 'ci:') && !startsWith(github.event.head_commit.message, 'docs:') }}
     steps:
@@ -90,7 +90,7 @@ jobs:
       fail-fast: false
       matrix:
         script: [ "test:e2e", "test:hmr", "test:umi" ]
-        os: [ ubuntu-latest ]
+        os: [ ubuntu-latest, macos-latest ]
     runs-on: ${{ matrix.os }} 
     steps:
       - uses: actions/checkout@v4
diff --git a/crates/binding/src/lib.rs b/crates/binding/src/lib.rs
index 8be4ab76b..309064015 100644
--- a/crates/binding/src/lib.rs
+++ b/crates/binding/src/lib.rs
@@ -173,6 +173,7 @@ pub struct BuildParams {
         ignoredPaths?: string[];
         _nodeModulesRegexes?: string[];
     };
+    caseSensitiveCheck?: boolean;
 }"#)]
     pub config: serde_json::Value,
     pub plugins: Vec<JsHooks>,
diff --git a/crates/mako/src/compiler.rs b/crates/mako/src/compiler.rs
index b4be7457b..6c1afc48c 100644
--- a/crates/mako/src/compiler.rs
+++ b/crates/mako/src/compiler.rs
@@ -273,6 +273,10 @@ impl Compiler {
                 },
             )));
         }
+        #[cfg(target_os = "macos")]
+        if config.case_sensitive_check {
+            plugins.push(Arc::new(plugins::case_sensitive::CaseSensitivePlugin::new()));
+        }
 
         if let Some(duplicate_package_checker) = &config.check_duplicate_package {
             plugins.push(Arc::new(
diff --git a/crates/mako/src/config.rs b/crates/mako/src/config.rs
index d554110a3..ac9181a18 100644
--- a/crates/mako/src/config.rs
+++ b/crates/mako/src/config.rs
@@ -220,6 +220,9 @@ pub struct Config {
         default
     )]
     pub check_duplicate_package: Option<DuplicatePackageCheckerConfig>,
+    // 是否开启 case sensitive 检查,只有mac平台才需要开启
+    #[serde(rename = "caseSensitiveCheck")]
+    pub case_sensitive_check: bool,
 }
 
 const CONFIG_FILE: &str = "mako.config.json";
diff --git a/crates/mako/src/config/mako.config.default.json b/crates/mako/src/config/mako.config.default.json
index 4f629661c..55975ffee 100644
--- a/crates/mako/src/config/mako.config.default.json
+++ b/crates/mako/src/config/mako.config.default.json
@@ -78,5 +78,6 @@
   "useDefineForClassFields": true,
   "emitDecoratorMetadata": false,
   "watch": { "ignorePaths": [], "_nodeModulesRegexes": [] },
-  "devServer": { "host": "127.0.0.1", "port": 3000 }
+  "devServer": { "host": "127.0.0.1", "port": 3000 },
+  "caseSensitiveCheck": false
 }
diff --git a/crates/mako/src/plugins.rs b/crates/mako/src/plugins.rs
index 53dc5f267..36de47d69 100644
--- a/crates/mako/src/plugins.rs
+++ b/crates/mako/src/plugins.rs
@@ -1,5 +1,6 @@
 pub mod async_runtime;
 pub mod bundless_compiler;
+pub mod case_sensitive;
 pub mod central_ensure;
 pub mod context_module;
 pub mod copy;
diff --git a/crates/mako/src/plugins/case_sensitive.rs b/crates/mako/src/plugins/case_sensitive.rs
new file mode 100644
index 000000000..cf8e649d2
--- /dev/null
+++ b/crates/mako/src/plugins/case_sensitive.rs
@@ -0,0 +1,95 @@
+use std::collections::HashMap;
+use std::fs;
+use std::path::Path;
+use std::sync::{Arc, Mutex};
+
+use anyhow::{anyhow, Result};
+
+use crate::ast::file::Content;
+use crate::compiler::Context;
+use crate::plugin::{Plugin, PluginLoadParam};
+
+pub struct CaseSensitivePlugin {
+    cache_map: Arc<Mutex<HashMap<String, Vec<String>>>>,
+}
+
+impl CaseSensitivePlugin {
+    pub fn new() -> Self {
+        CaseSensitivePlugin {
+            cache_map: Default::default(),
+        }
+    }
+
+    pub fn is_checkable(&self, load_param: &PluginLoadParam, root: &String) -> bool {
+        let file_path = &load_param.file.path;
+        if !file_path.starts_with(root) {
+            return false;
+        }
+        for component in file_path.iter() {
+            if component.eq_ignore_ascii_case("node_modules") {
+                return false;
+            }
+        }
+        true
+    }
+
+    pub fn check_case_sensitive(&self, file: &Path, root: &str) -> String {
+        // 可变变量，在循环内会被修改
+        let mut file_path = file.to_path_buf();
+        let mut case_name = String::new();
+        // 缓存map，file path做为key存在对应路径下的文件名和文件夹名
+        let mut cache_map = self.cache_map.lock().unwrap_or_else(|e| e.into_inner());
+        while file_path.to_string_lossy().len() >= root.len() {
+            if let Some(current) = file_path.file_name() {
+                let current_str = current.to_string_lossy().to_string();
+                file_path.pop(); // parent directory
+                let mut entries: Vec<String> = Vec::new();
+                if let Some(dir) = file_path.to_str() {
+                    if let Some(i) = cache_map.get(dir as &str) {
+                        entries = i.to_vec();
+                    } else if let Ok(files) = fs::read_dir(dir) {
+                        files.for_each(|entry| {
+                            entries.push(entry.unwrap().file_name().to_string_lossy().to_string());
+                        });
+                        cache_map.insert(dir.to_string(), entries.to_vec());
+                    }
+                }
+                if !entries.contains(&current_str) {
+                    if let Some(correct_name) = entries
+                        .iter()
+                        .find(|&x| x.to_lowercase() == current_str.to_lowercase())
+                    {
+                        case_name = correct_name.to_string();
+                        break;
+                    }
+                }
+            }
+        }
+        case_name
+    }
+}
+
+impl Plugin for CaseSensitivePlugin {
+    fn name(&self) -> &str {
+        "case_sensitive_plugin"
+    }
+
+    fn load(
+        &self,
+        load_param: &PluginLoadParam,
+        context: &Arc<Context>,
+    ) -> Result<Option<Content>> {
+        let root = &context.root.to_string_lossy().to_string();
+        if self.is_checkable(load_param, root) {
+            let dist_path = self.check_case_sensitive(load_param.file.path.as_path(), root);
+            if !dist_path.is_empty() {
+                return Err(anyhow!(
+                    "{} does not match the corresponding path on disk [{}]",
+                    load_param.file.path.to_string_lossy().to_string(),
+                    dist_path
+                ));
+            }
+        }
+        Ok(None)
+    }
+}
diff --git a/docs/config.md b/docs/config.md
index 06099781a..b60192e63 100644
--- a/docs/config.md
+++ b/docs/config.md
@@ -36,6 +36,22 @@ Whether to automatically enable CSS Modules.
 
 If not enabled, only files with `.module.css` or `.module.less` will be treated as CSS Modules; if enabled, named imports like `import styles from './a.css'` will also be treated as CSS Modules.
 
+### caseSensitiveCheck
+
+- Type: `boolean`
+- Default: `true`
+
+Whether to enable case-sensitive check.
+
+e.g.
+
+```ts
+{
+  caseSensitiveCheck: false,
+}
+```
+
+
 ### clean
 
 - Type: `boolean`
diff --git a/docs/config.zh-CN.md b/docs/config.zh-CN.md
index a32f5ed1c..1e4ca1132 100644
--- a/docs/config.zh-CN.md
+++ b/docs/config.zh-CN.md
@@ -36,6 +36,22 @@
 
 如果未启用，只有 `.module.css` 或 `.module.less` 的文件会被视为 CSS Modules；如果启用，像 `import styles from './a.css'` 这样的命名导入也会被视为 CSS Modules。
 
+### caseSensitiveCheck
+
+- 类型：`boolean`
+- 默认值：`true`
+
+是否启用大小写敏感检查。
+
+e.g.
+
+```ts
+{
+  caseSensitiveCheck: false,
+}
+```
+
+
 ### clean
 
 - 类型：`boolean`
@@ -850,3 +866,4 @@ babel-plugin-import 的简化版本，仅支持三个配置项：libraryName，l
 - 默认值：`true`
 
 是否在开发模式下将构建结果写入磁盘。
+
diff --git a/e2e/fixtures/error.case-sensitive/assets/umi-logo.png b/e2e/fixtures/error.case-sensitive/assets/umi-logo.png
new file mode 100644
index 000000000..f48e2ec78
Binary files /dev/null and b/e2e/fixtures/error.case-sensitive/assets/umi-logo.png differ
diff --git a/e2e/fixtures/error.case-sensitive/expect.js b/e2e/fixtures/error.case-sensitive/expect.js
new file mode 100644
index 000000000..c4a3e0464
--- /dev/null
+++ b/e2e/fixtures/error.case-sensitive/expect.js
@@ -0,0 +1,20 @@
+const assert = require("assert");
+const os = require("os");
+
+module.exports = (err) => {
+  if (os.platform() === "darwin") {
+    assert(
+      err.stderr.includes(
+        `/Assets/umi-logo.png does not match the corresponding path on disk [assets]`
+      ),
+      "should throw error"
+    );
+  } else {
+    assert(
+      err.stderr.includes(
+        `Module not found: Can't resolve './Assets/umi-logo.png'`
+      ),
+      "should throw error"
+    );
+  }
+};
diff --git a/e2e/fixtures/error.case-sensitive/index.ts b/e2e/fixtures/error.case-sensitive/index.ts
new file mode 100644
index 000000000..047f9291f
--- /dev/null
+++ b/e2e/fixtures/error.case-sensitive/index.ts
@@ -0,0 +1,4 @@
+import UmiLogo from "./Assets/umi-logo.png";
+console.log(UmiLogo);
+
+export default UmiLogo;
diff --git a/e2e/fixtures/error.case-sensitive/mako.config.json b/e2e/fixtures/error.case-sensitive/mako.config.json
new file mode 100644
index 000000000..79dde720d
--- /dev/null
+++ b/e2e/fixtures/error.case-sensitive/mako.config.json
@@ -0,0 +1,4 @@
+{
+  "mode": "production",
+  "caseSensitiveCheck": true
+}
diff --git a/e2e/fixtures/error.case-sensitive/package.json b/e2e/fixtures/error.case-sensitive/package.json
new file mode 100644
index 000000000..baffb4cb3
--- /dev/null
+++ b/e2e/fixtures/error.case-sensitive/package.json
@@ -0,0 +1,8 @@
+{
+  "name": "test",
+  "version": "1.0.0",
+  "dependencies": {
+    "a": "~1.0.0",
+    "b": "~1.0.0"
+  }
+}