diff --git a/packages/vite/src/node/__tests__/plugins/importGlob/__snapshots__/fixture.test.ts.snap b/packages/vite/src/node/__tests__/plugins/importGlob/__snapshots__/fixture.test.ts.snap
index 3efbb7a0306f86..6eeb763c4117bd 100644
--- a/packages/vite/src/node/__tests__/plugins/importGlob/__snapshots__/fixture.test.ts.snap
+++ b/packages/vite/src/node/__tests__/plugins/importGlob/__snapshots__/fixture.test.ts.snap
@@ -2,43 +2,43 @@
 
 exports[`fixture > transform 1`] = `
 "import * as __vite_glob_1_0 from \\"./modules/a.ts\\";import * as __vite_glob_1_1 from \\"./modules/b.ts\\";import * as __vite_glob_1_2 from \\"./modules/index.ts\\";import { name as __vite_glob_3_0 } from \\"./modules/a.ts\\";import { name as __vite_glob_3_1 } from \\"./modules/b.ts\\";import { name as __vite_glob_3_2 } from \\"./modules/index.ts\\";import { default as __vite_glob_5_0 } from \\"./modules/a.ts?raw\\";import { default as __vite_glob_5_1 } from \\"./modules/b.ts?raw\\";import \\"../../../../../../types/importMeta\\";
-export const basic = Object.assign({\\"./modules/a.ts\\": () => import(\\"./modules/a.ts\\"),\\"./modules/b.ts\\": () => import(\\"./modules/b.ts\\"),\\"./modules/index.ts\\": () => import(\\"./modules/index.ts\\")});
-export const basicEager = Object.assign({\\"./modules/a.ts\\": __vite_glob_1_0,\\"./modules/b.ts\\": __vite_glob_1_1,\\"./modules/index.ts\\": __vite_glob_1_2});
-export const ignore = Object.assign({\\"./modules/a.ts\\": () => import(\\"./modules/a.ts\\"),\\"./modules/b.ts\\": () => import(\\"./modules/b.ts\\")});
-export const namedEager = Object.assign({\\"./modules/a.ts\\": __vite_glob_3_0,\\"./modules/b.ts\\": __vite_glob_3_1,\\"./modules/index.ts\\": __vite_glob_3_2});
-export const namedDefault = Object.assign({\\"./modules/a.ts\\": () => import(\\"./modules/a.ts\\").then(m => m[\\"default\\"]),\\"./modules/b.ts\\": () => import(\\"./modules/b.ts\\").then(m => m[\\"default\\"]),\\"./modules/index.ts\\": () => import(\\"./modules/index.ts\\").then(m => m[\\"default\\"])});
-export const eagerAs = Object.assign({\\"./modules/a.ts\\": __vite_glob_5_0,\\"./modules/b.ts\\": __vite_glob_5_1});
-export const rawImportModule = Object.assign({\\"./modules/a.ts\\": () => import(\\"./modules/a.ts?raw\\"),\\"./modules/b.ts\\": () => import(\\"./modules/b.ts?raw\\")});
-export const excludeSelf = Object.assign({\\"./sibling.ts\\": () => import(\\"./sibling.ts\\")});
-export const customQueryString = Object.assign({\\"./sibling.ts\\": () => import(\\"./sibling.ts?custom\\")});
-export const customQueryObject = Object.assign({\\"./sibling.ts\\": () => import(\\"./sibling.ts?foo=bar&raw=true\\")});
-export const parent = Object.assign({});
-export const rootMixedRelative = Object.assign({\\"/css.spec.ts\\": () => import(\\"../../css.spec.ts?url\\").then(m => m[\\"default\\"]),\\"/define.spec.ts\\": () => import(\\"../../define.spec.ts?url\\").then(m => m[\\"default\\"]),\\"/esbuild.spec.ts\\": () => import(\\"../../esbuild.spec.ts?url\\").then(m => m[\\"default\\"]),\\"/import.spec.ts\\": () => import(\\"../../import.spec.ts?url\\").then(m => m[\\"default\\"]),\\"/importGlob/fixture-b/a.ts\\": () => import(\\"../fixture-b/a.ts?url\\").then(m => m[\\"default\\"]),\\"/importGlob/fixture-b/b.ts\\": () => import(\\"../fixture-b/b.ts?url\\").then(m => m[\\"default\\"]),\\"/importGlob/fixture-b/index.ts\\": () => import(\\"../fixture-b/index.ts?url\\").then(m => m[\\"default\\"])});
-export const cleverCwd1 = Object.assign({\\"./node_modules/framework/pages/hello.page.js\\": () => import(\\"./node_modules/framework/pages/hello.page.js\\")});
-export const cleverCwd2 = Object.assign({\\"./modules/a.ts\\": () => import(\\"./modules/a.ts\\"),\\"./modules/b.ts\\": () => import(\\"./modules/b.ts\\"),\\"../fixture-b/a.ts\\": () => import(\\"../fixture-b/a.ts\\"),\\"../fixture-b/b.ts\\": () => import(\\"../fixture-b/b.ts\\")});
+export const basic = /* #__PURE__ */ Object.assign({\\"./modules/a.ts\\": () => import(\\"./modules/a.ts\\"),\\"./modules/b.ts\\": () => import(\\"./modules/b.ts\\"),\\"./modules/index.ts\\": () => import(\\"./modules/index.ts\\")});
+export const basicEager = /* #__PURE__ */ Object.assign({\\"./modules/a.ts\\": __vite_glob_1_0,\\"./modules/b.ts\\": __vite_glob_1_1,\\"./modules/index.ts\\": __vite_glob_1_2});
+export const ignore = /* #__PURE__ */ Object.assign({\\"./modules/a.ts\\": () => import(\\"./modules/a.ts\\"),\\"./modules/b.ts\\": () => import(\\"./modules/b.ts\\")});
+export const namedEager = /* #__PURE__ */ Object.assign({\\"./modules/a.ts\\": __vite_glob_3_0,\\"./modules/b.ts\\": __vite_glob_3_1,\\"./modules/index.ts\\": __vite_glob_3_2});
+export const namedDefault = /* #__PURE__ */ Object.assign({\\"./modules/a.ts\\": () => import(\\"./modules/a.ts\\").then(m => m[\\"default\\"]),\\"./modules/b.ts\\": () => import(\\"./modules/b.ts\\").then(m => m[\\"default\\"]),\\"./modules/index.ts\\": () => import(\\"./modules/index.ts\\").then(m => m[\\"default\\"])});
+export const eagerAs = /* #__PURE__ */ Object.assign({\\"./modules/a.ts\\": __vite_glob_5_0,\\"./modules/b.ts\\": __vite_glob_5_1});
+export const rawImportModule = /* #__PURE__ */ Object.assign({\\"./modules/a.ts\\": () => import(\\"./modules/a.ts?raw\\"),\\"./modules/b.ts\\": () => import(\\"./modules/b.ts?raw\\")});
+export const excludeSelf = /* #__PURE__ */ Object.assign({\\"./sibling.ts\\": () => import(\\"./sibling.ts\\")});
+export const customQueryString = /* #__PURE__ */ Object.assign({\\"./sibling.ts\\": () => import(\\"./sibling.ts?custom\\")});
+export const customQueryObject = /* #__PURE__ */ Object.assign({\\"./sibling.ts\\": () => import(\\"./sibling.ts?foo=bar&raw=true\\")});
+export const parent = /* #__PURE__ */ Object.assign({});
+export const rootMixedRelative = /* #__PURE__ */ Object.assign({\\"/css.spec.ts\\": () => import(\\"../../css.spec.ts?url\\").then(m => m[\\"default\\"]),\\"/define.spec.ts\\": () => import(\\"../../define.spec.ts?url\\").then(m => m[\\"default\\"]),\\"/esbuild.spec.ts\\": () => import(\\"../../esbuild.spec.ts?url\\").then(m => m[\\"default\\"]),\\"/import.spec.ts\\": () => import(\\"../../import.spec.ts?url\\").then(m => m[\\"default\\"]),\\"/importGlob/fixture-b/a.ts\\": () => import(\\"../fixture-b/a.ts?url\\").then(m => m[\\"default\\"]),\\"/importGlob/fixture-b/b.ts\\": () => import(\\"../fixture-b/b.ts?url\\").then(m => m[\\"default\\"]),\\"/importGlob/fixture-b/index.ts\\": () => import(\\"../fixture-b/index.ts?url\\").then(m => m[\\"default\\"])});
+export const cleverCwd1 = /* #__PURE__ */ Object.assign({\\"./node_modules/framework/pages/hello.page.js\\": () => import(\\"./node_modules/framework/pages/hello.page.js\\")});
+export const cleverCwd2 = /* #__PURE__ */ Object.assign({\\"./modules/a.ts\\": () => import(\\"./modules/a.ts\\"),\\"./modules/b.ts\\": () => import(\\"./modules/b.ts\\"),\\"../fixture-b/a.ts\\": () => import(\\"../fixture-b/a.ts\\"),\\"../fixture-b/b.ts\\": () => import(\\"../fixture-b/b.ts\\")});
 "
 `;
 
 exports[`fixture > transform with restoreQueryExtension 1`] = `
 "import * as __vite_glob_1_0 from \\"./modules/a.ts\\";import * as __vite_glob_1_1 from \\"./modules/b.ts\\";import * as __vite_glob_1_2 from \\"./modules/index.ts\\";import { name as __vite_glob_3_0 } from \\"./modules/a.ts\\";import { name as __vite_glob_3_1 } from \\"./modules/b.ts\\";import { name as __vite_glob_3_2 } from \\"./modules/index.ts\\";import { default as __vite_glob_5_0 } from \\"./modules/a.ts?raw\\";import { default as __vite_glob_5_1 } from \\"./modules/b.ts?raw\\";import \\"../../../../../../types/importMeta\\";
-export const basic = Object.assign({\\"./modules/a.ts\\": () => import(\\"./modules/a.ts\\"),\\"./modules/b.ts\\": () => import(\\"./modules/b.ts\\"),\\"./modules/index.ts\\": () => import(\\"./modules/index.ts\\")});
-export const basicEager = Object.assign({\\"./modules/a.ts\\": __vite_glob_1_0,\\"./modules/b.ts\\": __vite_glob_1_1,\\"./modules/index.ts\\": __vite_glob_1_2});
-export const ignore = Object.assign({\\"./modules/a.ts\\": () => import(\\"./modules/a.ts\\"),\\"./modules/b.ts\\": () => import(\\"./modules/b.ts\\")});
-export const namedEager = Object.assign({\\"./modules/a.ts\\": __vite_glob_3_0,\\"./modules/b.ts\\": __vite_glob_3_1,\\"./modules/index.ts\\": __vite_glob_3_2});
-export const namedDefault = Object.assign({\\"./modules/a.ts\\": () => import(\\"./modules/a.ts\\").then(m => m[\\"default\\"]),\\"./modules/b.ts\\": () => import(\\"./modules/b.ts\\").then(m => m[\\"default\\"]),\\"./modules/index.ts\\": () => import(\\"./modules/index.ts\\").then(m => m[\\"default\\"])});
-export const eagerAs = Object.assign({\\"./modules/a.ts\\": __vite_glob_5_0,\\"./modules/b.ts\\": __vite_glob_5_1});
-export const rawImportModule = Object.assign({\\"./modules/a.ts\\": () => import(\\"./modules/a.ts?raw\\"),\\"./modules/b.ts\\": () => import(\\"./modules/b.ts?raw\\")});
-export const excludeSelf = Object.assign({\\"./sibling.ts\\": () => import(\\"./sibling.ts\\")});
-export const customQueryString = Object.assign({\\"./sibling.ts\\": () => import(\\"./sibling.ts?custom&lang.ts\\")});
-export const customQueryObject = Object.assign({\\"./sibling.ts\\": () => import(\\"./sibling.ts?foo=bar&raw=true&lang.ts\\")});
-export const parent = Object.assign({});
-export const rootMixedRelative = Object.assign({\\"/css.spec.ts\\": () => import(\\"../../css.spec.ts?url&lang.ts\\").then(m => m[\\"default\\"]),\\"/define.spec.ts\\": () => import(\\"../../define.spec.ts?url&lang.ts\\").then(m => m[\\"default\\"]),\\"/esbuild.spec.ts\\": () => import(\\"../../esbuild.spec.ts?url&lang.ts\\").then(m => m[\\"default\\"]),\\"/import.spec.ts\\": () => import(\\"../../import.spec.ts?url&lang.ts\\").then(m => m[\\"default\\"]),\\"/importGlob/fixture-b/a.ts\\": () => import(\\"../fixture-b/a.ts?url&lang.ts\\").then(m => m[\\"default\\"]),\\"/importGlob/fixture-b/b.ts\\": () => import(\\"../fixture-b/b.ts?url&lang.ts\\").then(m => m[\\"default\\"]),\\"/importGlob/fixture-b/index.ts\\": () => import(\\"../fixture-b/index.ts?url&lang.ts\\").then(m => m[\\"default\\"])});
-export const cleverCwd1 = Object.assign({\\"./node_modules/framework/pages/hello.page.js\\": () => import(\\"./node_modules/framework/pages/hello.page.js\\")});
-export const cleverCwd2 = Object.assign({\\"./modules/a.ts\\": () => import(\\"./modules/a.ts\\"),\\"./modules/b.ts\\": () => import(\\"./modules/b.ts\\"),\\"../fixture-b/a.ts\\": () => import(\\"../fixture-b/a.ts\\"),\\"../fixture-b/b.ts\\": () => import(\\"../fixture-b/b.ts\\")});
+export const basic = /* #__PURE__ */ Object.assign({\\"./modules/a.ts\\": () => import(\\"./modules/a.ts\\"),\\"./modules/b.ts\\": () => import(\\"./modules/b.ts\\"),\\"./modules/index.ts\\": () => import(\\"./modules/index.ts\\")});
+export const basicEager = /* #__PURE__ */ Object.assign({\\"./modules/a.ts\\": __vite_glob_1_0,\\"./modules/b.ts\\": __vite_glob_1_1,\\"./modules/index.ts\\": __vite_glob_1_2});
+export const ignore = /* #__PURE__ */ Object.assign({\\"./modules/a.ts\\": () => import(\\"./modules/a.ts\\"),\\"./modules/b.ts\\": () => import(\\"./modules/b.ts\\")});
+export const namedEager = /* #__PURE__ */ Object.assign({\\"./modules/a.ts\\": __vite_glob_3_0,\\"./modules/b.ts\\": __vite_glob_3_1,\\"./modules/index.ts\\": __vite_glob_3_2});
+export const namedDefault = /* #__PURE__ */ Object.assign({\\"./modules/a.ts\\": () => import(\\"./modules/a.ts\\").then(m => m[\\"default\\"]),\\"./modules/b.ts\\": () => import(\\"./modules/b.ts\\").then(m => m[\\"default\\"]),\\"./modules/index.ts\\": () => import(\\"./modules/index.ts\\").then(m => m[\\"default\\"])});
+export const eagerAs = /* #__PURE__ */ Object.assign({\\"./modules/a.ts\\": __vite_glob_5_0,\\"./modules/b.ts\\": __vite_glob_5_1});
+export const rawImportModule = /* #__PURE__ */ Object.assign({\\"./modules/a.ts\\": () => import(\\"./modules/a.ts?raw\\"),\\"./modules/b.ts\\": () => import(\\"./modules/b.ts?raw\\")});
+export const excludeSelf = /* #__PURE__ */ Object.assign({\\"./sibling.ts\\": () => import(\\"./sibling.ts\\")});
+export const customQueryString = /* #__PURE__ */ Object.assign({\\"./sibling.ts\\": () => import(\\"./sibling.ts?custom&lang.ts\\")});
+export const customQueryObject = /* #__PURE__ */ Object.assign({\\"./sibling.ts\\": () => import(\\"./sibling.ts?foo=bar&raw=true&lang.ts\\")});
+export const parent = /* #__PURE__ */ Object.assign({});
+export const rootMixedRelative = /* #__PURE__ */ Object.assign({\\"/css.spec.ts\\": () => import(\\"../../css.spec.ts?url&lang.ts\\").then(m => m[\\"default\\"]),\\"/define.spec.ts\\": () => import(\\"../../define.spec.ts?url&lang.ts\\").then(m => m[\\"default\\"]),\\"/esbuild.spec.ts\\": () => import(\\"../../esbuild.spec.ts?url&lang.ts\\").then(m => m[\\"default\\"]),\\"/import.spec.ts\\": () => import(\\"../../import.spec.ts?url&lang.ts\\").then(m => m[\\"default\\"]),\\"/importGlob/fixture-b/a.ts\\": () => import(\\"../fixture-b/a.ts?url&lang.ts\\").then(m => m[\\"default\\"]),\\"/importGlob/fixture-b/b.ts\\": () => import(\\"../fixture-b/b.ts?url&lang.ts\\").then(m => m[\\"default\\"]),\\"/importGlob/fixture-b/index.ts\\": () => import(\\"../fixture-b/index.ts?url&lang.ts\\").then(m => m[\\"default\\"])});
+export const cleverCwd1 = /* #__PURE__ */ Object.assign({\\"./node_modules/framework/pages/hello.page.js\\": () => import(\\"./node_modules/framework/pages/hello.page.js\\")});
+export const cleverCwd2 = /* #__PURE__ */ Object.assign({\\"./modules/a.ts\\": () => import(\\"./modules/a.ts\\"),\\"./modules/b.ts\\": () => import(\\"./modules/b.ts\\"),\\"../fixture-b/a.ts\\": () => import(\\"../fixture-b/a.ts\\"),\\"../fixture-b/b.ts\\": () => import(\\"../fixture-b/b.ts\\")});
 "
 `;
 
 exports[`fixture > virtual modules 1`] = `
-"Object.assign({\\"/modules/a.ts\\": () => import(\\"/modules/a.ts\\"),\\"/modules/b.ts\\": () => import(\\"/modules/b.ts\\"),\\"/modules/index.ts\\": () => import(\\"/modules/index.ts\\")})
-Object.assign({\\"/../fixture-b/a.ts\\": () => import(\\"/../fixture-b/a.ts\\"),\\"/../fixture-b/b.ts\\": () => import(\\"/../fixture-b/b.ts\\"),\\"/../fixture-b/index.ts\\": () => import(\\"/../fixture-b/index.ts\\")})"
+"/* #__PURE__ */ Object.assign({\\"/modules/a.ts\\": () => import(\\"/modules/a.ts\\"),\\"/modules/b.ts\\": () => import(\\"/modules/b.ts\\"),\\"/modules/index.ts\\": () => import(\\"/modules/index.ts\\")})
+/* #__PURE__ */ Object.assign({\\"/../fixture-b/a.ts\\": () => import(\\"/../fixture-b/a.ts\\"),\\"/../fixture-b/b.ts\\": () => import(\\"/../fixture-b/b.ts\\"),\\"/../fixture-b/index.ts\\": () => import(\\"/../fixture-b/index.ts\\")})"
 `;
diff --git a/packages/vite/src/node/plugins/importMetaGlob.ts b/packages/vite/src/node/plugins/importMetaGlob.ts
index aef833e3879888..f5b81005e446fa 100644
--- a/packages/vite/src/node/plugins/importMetaGlob.ts
+++ b/packages/vite/src/node/plugins/importMetaGlob.ts
@@ -438,7 +438,9 @@ export async function transformGlobImport(
 
           files.forEach((i) => matchedFiles.add(i))
 
-          const replacement = `Object.assign({${objectProps.join(',')}})`
+          const replacement = `/* #__PURE__ */ Object.assign({${objectProps.join(
+            ','
+          )}})`
           s.overwrite(start, end, replacement)
 
           return staticImports
diff --git a/playground/glob-import/__tests__/glob-import.spec.ts b/playground/glob-import/__tests__/glob-import.spec.ts
index 27c8dd716d0358..fb1cb867358549 100644
--- a/playground/glob-import/__tests__/glob-import.spec.ts
+++ b/playground/glob-import/__tests__/glob-import.spec.ts
@@ -1,4 +1,13 @@
-import { addFile, editFile, isBuild, page, removeFile, withRetry } from '~utils'
+import {
+  addFile,
+  editFile,
+  findAssetFile,
+  getColor,
+  isBuild,
+  page,
+  removeFile,
+  withRetry
+} from '~utils'
 
 const filteredResult = {
   './alias.js': {
@@ -158,3 +167,16 @@ if (!isBuild) {
     })
   })
 }
+
+test('tree-shake eager css', async () => {
+  expect(await getColor('.tree-shake-eager-css')).toBe('orange')
+  expect(await getColor('.no-tree-shake-eager-css')).toBe('orange')
+  expect(await page.textContent('.no-tree-shake-eager-css-result')).toMatch(
+    '.no-tree-shake-eager-css'
+  )
+
+  if (isBuild) {
+    const content = findAssetFile(/index\.\w+\.js/)
+    expect(content).not.toMatch('.tree-shake-eager-css')
+  }
+})
diff --git a/playground/glob-import/index.html b/playground/glob-import/index.html
index a0ac2d866699ae..85e9e98d2c5ae7 100644
--- a/playground/glob-import/index.html
+++ b/playground/glob-import/index.html
@@ -13,6 +13,10 @@ <h2>Relative raw</h2>
 <pre class="relative-glob-raw"></pre>
 <h2>Side effect</h2>
 <pre class="side-effect-result"></pre>
+<h2>Tree shake Eager CSS</h2>
+<p class="tree-shake-eager-css">Should be orange</p>
+<p class="no-tree-shake-eager-css">Should be orange</p>
+<pre class="no-tree-shake-eager-css-result"></pre>
 
 <script type="module" src="./dir/index.js"></script>
 <script type="module">
@@ -107,3 +111,10 @@ <h2>Side effect</h2>
   const notInvocation = neverInvoke
   import.meta.glob('/side-effect/**', { eager: true })
 </script>
+
+<script type="module">
+  import.meta.glob('/tree-shake.css', { eager: true })
+  const results = import.meta.glob('/no-tree-shake.css', { eager: true })
+  document.querySelector('.no-tree-shake-eager-css-result').textContent =
+    results['/no-tree-shake.css'].default
+</script>
diff --git a/playground/glob-import/no-tree-shake.css b/playground/glob-import/no-tree-shake.css
new file mode 100644
index 00000000000000..9075733e138c5a
--- /dev/null
+++ b/playground/glob-import/no-tree-shake.css
@@ -0,0 +1,3 @@
+.no-tree-shake-eager-css {
+  color: orange;
+}
diff --git a/playground/glob-import/tree-shake.css b/playground/glob-import/tree-shake.css
new file mode 100644
index 00000000000000..84f24297e4efce
--- /dev/null
+++ b/playground/glob-import/tree-shake.css
@@ -0,0 +1,3 @@
+.tree-shake-eager-css {
+  color: orange;
+}