diff --git a/drivers/i2s/Kconfig.stm32 b/drivers/i2s/Kconfig.stm32
index e6b35e8561bd2..697aee3b08462 100644
--- a/drivers/i2s/Kconfig.stm32
+++ b/drivers/i2s/Kconfig.stm32
@@ -36,7 +36,7 @@ menuconfig I2S_STM32_SAI
 	select USE_STM32_HAL_DMA_EX
 	select USE_STM32_HAL_SAI
 	help
-	  Enable SAI support on the STM32U5 family of processors.
+	  Enable SAI support on the STM32 family of processors.
 
 if I2S_STM32_SAI
 
diff --git a/dts/arm/st/h5/stm32h5.dtsi b/dts/arm/st/h5/stm32h5.dtsi
index 3fcd51acff019..f577fa0f2ddd6 100644
--- a/dts/arm/st/h5/stm32h5.dtsi
+++ b/dts/arm/st/h5/stm32h5.dtsi
@@ -633,6 +633,28 @@
 			status = "disabled";
 		};
 
+		sai1_a: sai1@40015404 {
+			compatible = "st,stm32-sai";
+			#address-cells = <1>;
+			#size-cells = <0>;
+			reg = <0x40015404 0x20>;
+			clocks = <&rcc STM32_CLOCK(APB2, 21)>,
+				 <&rcc STM32_SRC_PLL2_P SAI1_SEL(0)>;
+			dmas = <&gpdma1 1 53 0>;
+			status = "disabled";
+		};
+
+		sai1_b: sai1@40015424 {
+			compatible = "st,stm32-sai";
+			#address-cells = <1>;
+			#size-cells = <0>;
+			reg = <0x40015424 0x20>;
+			clocks = <&rcc STM32_CLOCK(APB2, 21)>,
+				 <&rcc STM32_SRC_PLL2_P SAI1_SEL(0)>;
+			dmas = <&gpdma1 0 54 0>;
+			status = "disabled";
+		};
+
 		usb: usb@40016000 {
 			compatible = "st,stm32-usb";
 			reg = <0x40016000 0x400>;
diff --git a/samples/drivers/i2s/output/boards/nucleo_h563zi.conf b/samples/drivers/i2s/output/boards/nucleo_h563zi.conf
new file mode 100644
index 0000000000000..4f3f73a1e06a5
--- /dev/null
+++ b/samples/drivers/i2s/output/boards/nucleo_h563zi.conf
@@ -0,0 +1 @@
+CONFIG_HEAP_MEM_POOL_SIZE=4192
diff --git a/samples/drivers/i2s/output/boards/nucleo_h563zi.overlay b/samples/drivers/i2s/output/boards/nucleo_h563zi.overlay
new file mode 100644
index 0000000000000..561db541c6ee2
--- /dev/null
+++ b/samples/drivers/i2s/output/boards/nucleo_h563zi.overlay
@@ -0,0 +1,33 @@
+/*
+ * Copyright (c) 2025 ZAL Zentrum für Angewandte Luftfahrtforschung GmbH
+ * Copyright (c) 2025 Mario Paja
+ *
+ * SPDX-License-Identifier: Apache-2.0
+ */
+
+/ {
+	aliases {
+		i2s-tx = &sai1_a;
+	};
+};
+
+&pll2 {
+	/* 44.1KHz (0.09% Error) */
+	div-m = <2>;
+	mul-n = <113>;
+	div-q = <2>;
+	div-r = <2>;
+	div-p = <2>;
+	clocks = <&clk_hse>;
+	status = "okay";
+};
+
+&sai1_a {
+	pinctrl-0 = <&sai1_mclk_a_pe2 &sai1_sd_a_pe6
+		&sai1_fs_a_pe4 &sai1_sck_a_pe5>;
+	pinctrl-names = "default";
+	status = "okay";
+	mclk-enable;
+	mclk-divider = "div-256";
+	dma-names = "tx";
+};