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Refinement or Post-Process Maps #54
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Hi Matt, We observe the best results after automated global sharpening with Relion. I would imagine the same to be true for CS. Generally, over-sharpening seems to hurt us more than under-sharpening. DeepEMhancer, in my hands, seems to make things more difficult for ModelAngelo. This is all related to how we trained ModelAngelo, which is with postprocessed maps in the EMDB. Best, |
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Hi Kiarash,
Can you provide a suggestion on which volume to use for ModelAngelo? My results vary when using maps from a refinement job (I’m using CS), a post phenix Resolve CryoEM job, and a post DeepEMhancer job. In general, I see the best results with a map from a refinement job. Do you see the same? If so, is this due to the datasets you train your neural network on?
Thanks,
Matt
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