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Cannot re-extract particles with manually refined particle coordinates (Relion 3.1) #1233

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dj255-cages opened this issue Jan 28, 2025 · 1 comment

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@dj255-cages
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dj255-cages commented Jan 28, 2025

After some 2D classification, I ran a particle extraction job to refine my particles. Then, I opened the particle picking GUI by displaying the "coords_suffix_extract.star" file. After manually adding/removing and re-centering some particles, I saved the coords.star with the new coordinates. However, when I try to re-extract particles from the same micrographs with the updated coordinates, I get a "ObersvationModel::getBoxSize: box sizes not available" error.

micrographs.star:

version 30001

data_optics

loop_
_rlnOpticsGroupName #1
_rlnOpticsGroup #2
_rlnMicrographOriginalPixelSize #3
_rlnVoltage #4
_rlnSphericalAberration #5
_rlnAmplitudeContrast #6
_rlnMicrographPixelSize #7
opticsGroup1 1 1.070000 200.000000 2.700000 0.100000 1.070000

version 30001

data_micrographs

loop_
_rlnMicrographName #1
_rlnOpticsGroup #2
_rlnCtfImage #3
_rlnDefocusU #4
_rlnDefocusV #5
_rlnCtfAstigmatism #6
_rlnDefocusAngle #7
_rlnCtfFigureOfMerit #8
_rlnCtfMaxResolution #9

coords_suffix_extract.star:
Select/job005/micrographs.star

@biochem-fan
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biochem-fan commented Jan 28, 2025

We no longer support RELION 3.1; please use the latest version.

Moreover, similar issues have been discussed in the CCPEM mailing list before.

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