output generated by "relion_flex_analyse" missing?

[ncoudray]

Hi,

I was running the following command "relion_flex_analyse" to write out separate STAR files for particles images for which the amplitude along the first eigenvector is within a certain range (as in Fig 2E of https://elifesciences.org/articles/36861#fig2). This is the command I use:

Environment:

• OS: Rocky Linux 8.8
• RELION version relion/5.0.0_cu11.8 (from SBGrid)
• Memory: tried with 80G
• GPU: none used for this command

Dataset:

• Box size: 256px
• Pixel size: 1.083 Å/px
• Number of particles: 378,252

Job options:

• Type of job: relion_flex_analyse after multi-body refinement
• Number of MPI processes: 1
• Number of threads: 20
• Full command

`relion_flex_analyse --data  MultiBody/job097/run_data.star  --model MultiBody/job097//run_model.star  --bodies MultiBody_3_2bodies.star --o MultiBody/job097/extract/ex1 --select_eigenvalue 1 --select_eigenvalue_min -100 --select_eigenvalue_max 100`

(I tried with and without the " --write_pca_projections" option)

Error message:

No error message, but only those outputs generated:

MultiBody/job097/extract/ex1_body001_mask.mrc 
MultiBody/job097/extract/ex1_body002_mask.mrc
MultiBody/job097/extract/ex1:

I don't see any star file, but no error message. However, the stdout file ends with:
..~~(,_,"> yum!

which may be indicative of what's wrong

Thanks,
Best,
Nicolas