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retrieve_seq_from_fasta_usage.txt
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retrieve_seq_from_fasta_usage.txt
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Usage:
retrieve_seq_from_fasta.pl [arguments] <regionfile>
Optional arguments:
-v, --verbose use verbose output
-h, --help print help message
-m, --man print complete documentation
--format <string> format of the regionfile
--outfile <file> output file name (default: input.fa)
--seqdir <directory> directory containing sequence files for genomes
--seqfile <file> sequence file name
--tabout use tab-delimited output (default is FASTA file)
--altchr process alternative haplotype chromosome (default: OFF)
--[no]addchr add chr prefix to region specification (default: ON)
Function: reformat sequences at specific genomic positions from whole-genome FASTA files
Example: retrieve_seq_from_fasta.pl -format tab -seqdir humandb/ region.tabinput
retrieve_seq_from_fasta.pl -format tab -seqfile chrall.fa region.tabinput
retrieve_seq_from_fasta.pl -format simple -seqdir humandb/ region.input
retrieve_seq_from_fasta.pl -format refGene -seqdir humandb hg18_refGenet.txt
retrieve_seq_from_fasta.pl -format genericGene -seqdir humandb/ hg18_gencodeGene.txt
Version:$Date: 2015-12-14 13:51:19 -0800 (Mon, 14 Dec 2015) $
Options:
--help print a brief help message and exit
--man print the complete manual of how to use the program
--verbose
use verbose output
--format
specify the format of the input file (default: tab). Other
supported formats include simple, refGene, knownGene, ensGene,
genericGene and many other format support will be added in the
future.
--outfile
specify the output file name (default: <input>.fa)
--seqdir
specify the directory of the sequence files (usually these
sequence files are organized by chromosomes, and that the file
name represent the chromosome).
--seqfile
specify the sequence file name (assuming that all sequences are
stored within a single file).
--tabout
use tab-delimited output. By default, FASTA file will be printed
out.
--altchr
process alternative haplotype chromosome, that look like
chr6_cox_hap1. By default this will not be processed.
--(no)addchr
add chr prefix to region if it is not present (default: ON)