The format is based on Keep a Changelog and this project adheres to Semantic Versioning.
- Changed downsample strategy in
modules/local/downsample_rate.nf
that was causing differences with results from geneflow version. Downsample rate now set at default 1 (closes #67)
- Changed minimum time requirements in
base.config
forprocess_low
,process_medium
,process_high
to 72.h andprocess_long
to 120.h. - SNP distance matrix addition as output.
- Updated
qc_report.txt
to include coverage mean depth and reads mapped. - Positions masked
(N)
based on DP & Added functionality to usemin_depth
(Default 50). - Change
test
profile to includemin_depth = 2
so it will run to completion.
- Bug fix for downsample mismatch.
- Change configuration variable name from vcftools_filter to
gatkgenotypes_filter
. - Changed samplesheet creation to accept multiple directories as arguments and to recursively search for sequences.
- Set full vcf consensus file to debug output
- Remove part nf-core branding
- Update logo
- Fixed bug in
modules/local/lane_merge.nf
that was causing samplesheet CSV file to not recognize R2 (closes #39) - Formatting of
docs/output.md
- Changed output file
combined/vcf-to-fasta/combined_vcf-to-fasta.fasta
->combined/vcf-to-fasta/vcf-to-fasta.fasta
- Output file
combined/vcf-to-fasta/vcf-to-fasta.fasta
will now replace stars*
with dashes-
- Output file
combined/phylogeny/rapidnj/rapidnj_phylogeny.tre
->combined/phylogeny/rapidnj/rapidnj_phylogeny.nh
- Output file
combined/phylogeny/iqtree/vcf-to-fasta.fasta.treefile
->combined/phylogeny/iqtree/iqtree_phylogeny.nh
- Output file
combined/phylogeny/raxmlng/output.raxml.bestTree
->combined/phylogeny/raxmlng/raxmlng_bestTree.nh
- Output file
combined/phylogeny/raxmlng/output.raxml.support
->combined/phylogeny/raxmlng/raxmlng_support.nh
/results/qc
output dir removed
- Continue improving output docs
Initial release of CDCgov/mycosnp-nf, created with the nf-core template.
- Added
test
andtest_full
profiles for easy testing - Support for phylogenetic tree generation
--rapidnj
--fasttree
--iqtree
--raxmlng
- Skip reference generation
--ref_dir
|assets/precomputed/reference
available for testing - Add SRA ids to download from NCBI
--add_sra_file
|assets/sra_small.csv
assets/sra_large.csv
available for testing - Add vcf files generated from previous runs
--add_vcf_file
|assets/precomputed/vcf
available for testing - Skip combined variant analysis (run reference prep and mapping)
skip_combined_analysis
- Skip samples capability
--skip_samples
- Skip samples file capability
--skip_samples_file
- Nextflow
- nf-core
- GeneFlow support
- QC pdf report
- Intermediate file cleanup and management
- Update logo and metro-style workflow