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FRAPLaunchpad.m
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FRAPLaunchpad.m
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function FRAPLaunchpad(handles, credentials)
% Copyright (C) 2013-2014 University of Dundee & Open Microscopy Environment.
% All rights reserved.
%
% This program is free software; you can redistribute it and/or modify
% it under the terms of the GNU General Public License as published by
% the Free Software Foundation; either version 2 of the License, or
% (at your option) any later version.
%
% This program is distributed in the hope that it will be useful,
% but WITHOUT ANY WARRANTY; without even the implied warranty of
% MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
% GNU General Public License for more details.
%
% You should have received a copy of the GNU General Public License along
% with this program; if not, write to the Free Software Foundation, Inc.,
% 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301 USA.
[images imageIds imageNames roiShapes datasetNames pixels] = FRAPChooser;
if isempty(images)
return;
end
numImages = length(imageIds);
progBar = waitbar(0, 'Analysing...');
for thisImage = 1:numImages
[roiShapes{thisImage} indices{thisImage}] = FRAPMeasure(images.get(thisImage-1), imageIds(thisImage), imageNames{thisImage}, roiShapes{thisImage}, datasetNames{thisImage}, pixels{thisImage});
waitbar(thisImage/numImages, progBar);
end
close(progBar);
writeDataOut(roiShapes, indices, datasetNames);
gatewayDisconnect
function writeDataOut(roiShapes, indices, datasetNames)
numImages = length(roiShapes);
dataOut = [];
dataSummary = {'File Name', 'Dataset', 'T1/2', 'Mobile Fraction', 'Immobile Fraction'};
for thisImage = 1:numImages
dataOut = [dataOut; {[roiShapes{thisImage}{indices{thisImage}.frapIdx}.name, ' Frap analysis. T1/2 = ', num2str(roiShapes{thisImage}{indices{thisImage}.frapIdx}.Thalf), 's, mobile fraction = ', num2str(roiShapes{thisImage}{indices{thisImage}.frapIdx}.mobileFraction), ', immobile fraction = ', num2str(roiShapes{thisImage}{indices{thisImage}.frapIdx}.immobileFraction)],'','','','','',''}];
dataOut = [dataOut; {'File Name', 'Dataset', 'Timestamp', 'Frap Intensities', 'Base Intensities', 'Whole Intensities', 'Frap Normalised Corrected';}];
for thisTimestamp = 1:length(roiShapes{thisImage}{indices{thisImage}.frapIdx}.correctT)
dataOut = [dataOut; {roiShapes{thisImage}{indices{thisImage}.frapIdx}.name datasetNames{thisImage} num2str(roiShapes{thisImage}{indices{thisImage}.frapIdx}.timestamp(thisTimestamp))} roiShapes{thisImage}{indices{thisImage}.frapIdx}.frapData(thisTimestamp) roiShapes{thisImage}{indices{thisImage}.baseIdx}.baseData(thisTimestamp) roiShapes{thisImage}{indices{thisImage}.wholeIdx}.wholeData(thisTimestamp) roiShapes{thisImage}{indices{thisImage}.frapIdx}.frapNormCorr(thisTimestamp)];
end
dataOut = [dataOut; {' ',' ',' ',' ',' ',' ',' '}];
dataSummary = [dataSummary; {roiShapes{thisImage}{indices{thisImage}.frapIdx}.name, datasetNames{thisImage}, num2str(roiShapes{thisImage}{indices{thisImage}.frapIdx}.Thalf), num2str(roiShapes{thisImage}{indices{thisImage}.frapIdx}.mobileFraction), num2str(roiShapes{thisImage}{indices{thisImage}.frapIdx}.immobileFraction)}];
end
[saveFile savePath] = uiputfile('*.xls','Save Results','/FrapAnalysisResults.xls');
if isnumeric(saveFile) && isnumeric(savePath)
return;
end
try
xlswrite([savePath saveFile], dataOut, 'Data All Timepoints');
xlswrite([savePath saveFile], dataSummary, 'Data Summary');
catch
%If the xlswriter fails (no MSOffice installed, e.g.) then manually
%create a .csv file. Turn every cell to string to make it easier.
[rows cols] = size(dataOut);
for thisRow = 1:rows
for thisCol = 1:cols
if isnumeric(dataOut{thisRow, thisCol})
dataOut{thisRow, thisCol} = num2str(dataOut{thisRow, thisCol});
end
end
end
[rowsSummary colsSummary] = size(dataSummary);
for thisRow = 1:rowsSummary
for thisCol = 1:colsSummary
if isnumeric(dataSummary{thisRow, thisCol})
dataSummary{thisRow, thisCol} = num2str(dataSummary{thisRow, thisCol});
end
end
end
delete([savePath saveFile]); %Delete the .xls file and save again as .csv
[savePart remain] = strtok(saveFile, '.');
saveFile = [savePart '.csv'];
saveFileSummary = [savePart 'Summary.csv'];
%Write out DataOut to file
fid = fopen([savePath saveFile], 'w');
for thisRow = 1:rows
for thisCol = 1:cols
fprintf(fid, '%s', dataOut{thisRow, thisCol});
fprintf(fid, '%s', ',');
end
fprintf(fid, '%s\n', '');
end
fclose(fid);
%Write out dataSummary to file
fid = fopen([savePath saveFileSummary], 'w');
for thisRow = 1:rowsSummary
for thisCol = 1:colsSummary
fprintf(fid, '%s', dataSummary{thisRow, thisCol});
fprintf(fid, '%s', ',');
end
fprintf(fid, '%s\n', '');
end
fclose(fid);
end