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Generating reads from multiple draft genomes - output file not found error for concatenate #275

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sreerampeela opened this issue Jan 8, 2025 · 0 comments

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@sreerampeela
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sreerampeela commented Jan 8, 2025

Hi,

I am using the below command to simulate reads from 3 draft genomes in separate multi-FASTA files, where each FASTA file represents one genome. The contig ids in each file are different from the FASTA file name.

iss generate --seed 1987 --quiet --cpus 10 -b genome_abundance.txt \
--draft VB103.fasta --draft VB3985.fasta --draft VB7185.fasta \
-U 3 --n_reads 1M -m HiSeq -z -l 124 --fragment-length-sd 25  -o meta_testing3

genome_abundance.txt:
VB103 0.4
VB3985 0.2
VB7185 0.4

The error traceback (attached file) has been traced using '--debug' option along with the above code

I would like to know about pre-processing draft FASTA files and how to specify abundances for each sample (and not each contig in the draft genome). Also can we specify the draft genomes in a single text file along with absolute paths?
iss_error.log
.

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