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0.7.0 (in progress)

  • Fix for kallisto output failing due to defaultdict not being imported. Thanks to @andreas-wilm for the fix.
  • Added tagcount option --parse_tags to use BAM tags rather than parsing read names (UM for UMI, CR for cell barcode)
  • Added tagcount option --gene_tags to use BAM tags to get ID of mapping gene (GX tag).

0.6.0

  • Fix skipping first piece of evidence when tagcounting.
  • Add test for tagcount.
  • Output full sorted transcript table from tagcount rather than only the observed transcripts.
  • Add --sparse option to output tagcount matrices in MatrixMarket format.
  • Allow cb_histogram subcommand to take gzipped files.
  • Allow cb_filter subcommand to take gzipped files.
  • Add support for triple-cellular barcodes.
  • Add example for Illumina SureCell (https://www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/surecell-wta-ddseq.html)

0.5.0

  • Fix automatic format detection in cb_histogram.
  • Add tests for cb_histogram.
  • Re-enable streaming bamtagging. Thanks to @chapmanb for the suggestion.
  • Add subset_bamfile to subset a BAM file to keep alignments with a given set of cellular barcodes.
  • Speed improvements for reading gzipped FASTQ files.
  • Memory usage improvements for tagcount.

0.4.0

  • Fix for handling unicode, thanks to @chapmanb and @sowmyaiyer
  • Adds support for adding BAM tags to aligned fastqtransformed files. Thanks to @chapmanb.
  • Adds support for UMI-only fastqtransformation.
  • Adds support for paired-end target sequences.
  • Adds support for detecting sample barcodes via the SB tag in the regex.
  • Adds support for sample-based demultiplexing with error correction.

0.3.0

  • Now supports transforming 3-file input, as from the Linnarsson lab STRT-Seq data
  • New kallisto subcommand formats read files for input to kallisto's UMI mode
  • Fix gzip based fastq reading on Python 3.5
  • Including preliminary subcommand for guessing cell cutoff from cb_histogram

0.2.2

  • Added MANIFEST file which broke pip installation