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SQUID Error - Segmentation fault (core dumped) #28

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think-o opened this issue Sep 29, 2021 · 0 comments
Open

SQUID Error - Segmentation fault (core dumped) #28

think-o opened this issue Sep 29, 2021 · 0 comments

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@think-o
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think-o commented Sep 29, 2021

Hello!
I get this error while running Squid. The chimeric sam file generated through STAR run is empty and it fails when run as a bam file with Squid. Please help me to sort this error out. Is it because the chimeric reads are not detected for the datasets I used?

/home/software/STAR-2.7.9a/bin/Linux_x86_64/STAR --runThreadN 55 --genomeDir /home/genome_files/CTAT_Library/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --readFilesIn /home/results/read_1_trim.fastq /home/results/read_2_trim.fastq --outFileNamePrefix /home/results/star_run. --outSAMtype BAM SortedByCoordinate --outReadsUnmapped Fastx --chimSegmentMin 20 --outSAMstrandField intronMotif --chimOutType SeparateSAMold
        STAR version: 2.7.9a   compiled: 2021-05-04T09:43:56-0400 vega:/homedobin/data/STAR/STARcode/STAR.master/source
Sep 28 18:13:51 ..... started STAR run
Sep 28 18:13:51 ..... loading genome
Sep 28 18:13:53 ..... started mapping
Sep 28 18:14:21 ..... finished mapping
Sep 28 18:14:21 ..... started sorting BAM
Sep 28 18:14:22 ..... finished successfully
Reference name 1        -->     0
Reference name 2        -->     1
Reference name 3        -->     2
Reference name 4        -->     3
Reference name 5        -->     4
Reference name Mt       -->     5
Reference name Pt       -->     6
[Tue Sep 28 18:14:23 2021] Start reading bam file.
[Tue Sep 28 18:14:23 2021] Finish sorting Chimeric bam reads.
[Tue Sep 28 18:14:23 2021] Finish removing PCR duplicates.
[Tue Sep 28 18:14:23 2021] Building nodes. |bamdiscordant|=0
[Tue Sep 28 18:14:23 2021] Building nodes, finish seeding.
../scripts/no_bloom_fusion_tools.sh: line 103: 17949 Segmentation fault      (core dumped) $SQUID -b $run_dir/star_run.Aligned.sortedByCoord.out.bam -c $run_dir/star_run.Chimeric.out.bam -o $run_dir/squid_out
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