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data_acquisition.md

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Data acquisition info

IR ILLUMINATION:

For recording in darkness we use IR illumination at 850nm, which works well with 2p imaging at 970nm and even 920nm. Depending on your needs, you might want to choose a different wavelength, which changes all the filters below as well. 950nm works just as well, and probably so does 750nm, which still outside of the visible range for rodents.

If you want to focus the illumination on the mouse eye or face, you will need a different, more expensive system. Here is an example, courtesy of Michael Krumin from the Carandini lab: driver, power supply, LED, lens, and lens tube, and another lens tube.

CAMERAS:

We use ptgrey cameras. The software we use for simultaneous acquisition from multiple cameras is BIAS software. A basic lens that works for zoomed out views here. To see the pupil well you might need a better zoom lens 10x here.

For 2p imaging, you'll need a tighter filter around 850nm so you don't see the laser shining through the mouse's eye/head, for example this. Depending on your lenses you'll need to figure out the right adapter(s) for such a filter. For our 10x lens above, you might need all of these: adapter1, adapter2, adapter3, adapter4.