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Metabolomics Results for OSM Series 1, 3 and 4 #524
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I made a quick and dirty graphic describing the data collected from the experiment: @mattodd |
OSM-S-5 almost looks like a surfactant @spadavec can you model what this thing does in membrane? |
Thanks for the graphic @MFernflower but we lost some of the OSM codes. @alintheopen any chance you could drag the zip of the .cdx here from your original post here? Here's the excel. |
@mattodd updated graphic: |
@MFernflower Not really--it would be very exploratory MD, which is something that I don't really feel comfortable with. There might be a fun way to calculate dG_insertion into a membrane for that compound (assuming its passive transport), but not sure how informative that would be. |
@MFernflower Out of curiosity which surfactant do you feel they look similar to? Most I know of have an ionisable group. |
I would assume the free N atom on the tail would ionize while the front of
the molecule acts as a hydrophobic head
https://user-images.githubusercontent.com/3164942/28756576-2db889e8-753f-11e7-8837-8756b2e3af58.png
Update: I totally forgot how difficult it would be to ionize that terminal amide at normal parasite PH - do you guys know someone who can run a MD simulation of OSM-S-5 verses a cell membrane?
@drc007 @mattodd
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I bumped into Darren, Anubhav and Stuart Ralph in Melbourne last week. We talked about these results. Points to note:
Subsequent edit: Darren Creek asks this: "OSM-S-37 had the strongest impact on pyrimidine synthesis in our assay, and OSM-S-112 was a weaker inhibitor... is this in line with their anti-parasitic IC50s? or opposite? If in line then I suggest Stuart use OSM-S-37, but if opposite then he should test both (if that is the case then perhaps OSM-S-112 hits multiple targets and the DHODH is secondary... although I'd be really surprised if OSM-S-37 has no activity because it inhibits pyrimidine synthesis as effectively as atovaquone in our assay." Answer: Potencies are shown graphically here. OSM-S-37 is highly potent vs parasite, OSM-S-112 is much less so. So this is in line with results vs. pyrimidine synthesis. i.e. OSM-S-37 and OSM-S-5 will be prioritised by Darren and Anubhav when Stuart is ready for the DHODH assays.
(3a - Separately we have received approval to send some OSM-S-106 to Elizabeth Winzeler's lab for MoA analysis, presumably by the creation of resistant mutants. More on this when we send compound and know about timelines - it's an in-kind support from MMV in response to their recent call for compounds to be investigated further in this way. Communicated to me by email a couple of weeks ago).
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Now that you say it - OSM-S-112 does indeed look like it has potential to be a redox cycler - I wonder how that would be tested in a lab however - some sort of eletrochemical cell? @mattodd |
Is there interest in following up on OSM-S-37? |
I'd be curious if you could dock it to DHODH so we could have some sort of model? @holeung |
Just FYI there was a mixup of codes used in the above (re OSM-S-313 and OSM-S-291) - see OpenSourceMalaria/OSMSeries4Paper1#36 |
Darren Creek and his lab have carried out metabolomics work on several OSM compounds (see #469 for background). Darren constructed a powerpoint of the results (below) and provided the following commentary, which I’m posting with permission.
“We have tested those OSM compounds in our metabolomics screen and have some nice results regarding the Mode of Action of two of the actives. A full summary is attached, briefly:
OSM-S-37 strongly (and OSM-S-112 weakly) inhibit pyrimidine biosynthesis, suggesting a MoA similar to atovaquone or DSM-265.
OSM-S-313 looks like a PfATP4 inhibitor, and matches the profiles of the three compounds MMV sent representing this class.
OSM-S-5 and OSM-S-106 had significant effects on the parasite in our 5-hour assay, but gave a 'general death' profile which is not class-specific, so we can't pinpoint a target. We could repeat these two compounds on purified parasites to dig a bit deeper.
OSM-S-4, OSM-S-133 and OSM-S-291 had no impact on parasite metabolism in our assay. I assume these (and OSM-S-112) were the negative controls?
Perhaps we could arrange a TC to discuss the results and where to from here? I am interested to follow-up 5 and 106 in more depth, but it would be good to know more context about the background and progress of these classes before investing too much into it.”
Interest in MoA for Series 3 (OSM-S-106) is described in #421.
Competition re Series 4 (OSM-S-313) is described in #503.
Publication of Series 1 (OSM-S-5, OSM-S-37) (#434) was inconclusive on MoA
This is nice, and important, work. I guess the results support the idea that Series 4 targets PfATP4. We have a possible line of enquiry for Series 1. We'll need more investigations to provide clues for Series 3. Any thoughts/suggestions below.
Data_summary_OSM_MMV_compounds.pptx
Data also posted here.
(This Results Issue can be closed once these data are incorporated into relevant wikis to ensure no orphaning.)
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