You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
{{ message }}
This repository has been archived by the owner on Mar 16, 2022. It is now read-only.
HI, I run Falcon on pacbio reads successfully, but failed to run falcon-unzip. Here is the error report:
It seems that errors occurred at this step 3-unzip/1-hasm. The file 'preads.p_ovl' is empty. could you please help me to solve this issue? Many thanks.
The log information in file 'run-P5d87a9de2f785c.bash.stderr' under this folder '3-unzip/1-hasm'
executable=${PYPEFLOW_JOB_START_SCRIPT}
+ executable=/export/scratch3/vincent/project/TGS_assembly/other_assemblers/clr/mhc/25x/falcon
unzip/3-unzip/1-hasm/run-P5d87a9de2f785c.bash
timeout=${PYPEFLOW_JOB_START_TIMEOUT:-60} # wait 60s by default
+ timeout=60
# Wait up to timeout seconds for the executable to become "executable",
# then exec.
#timeleft = int(timeout)
while [[ ! -x "${executable}" ]]; do
if [[ "${timeout}" == "0" ]]; then
echo "timed out waiting for (${executable})"
exit 77
fi
echo "not executable: '${executable}', waiting ${timeout}s"
sleep 1
timeout=$((timeout-1))
done
+ [[ ! -x /export/scratch3/vincent/project/TGS_assembly/other_assemblers/clr/mhc/25x/falconunz
ip/3-unzip/1-hasm/run-P5d87a9de2f785c.bash ]]
/bin/bash ${executable}
+ /bin/bash /export/scratch3/vincent/project/TGS_assembly/other_assemblers/clr/mhc/25x/falconu
nzip/3-unzip/1-hasm/run-P5d87a9de2f785c.bash
+ '[' '!' -d /export/scratch3/vincent/project/TGS_assembly/other_assemblers/clr/mhc/25x/falcon
unzip/3-unzip/1-hasm ']'
+ cd /export/scratch3/vincent/project/TGS_assembly/other_assemblers/clr/mhc/25x/falconunzip/3-
unzip/1-hasm
+ eval '/bin/bash run.sh'
++ /bin/bash run.sh
export PATH=$PATH:/bin
cd /export/scratch3/vincent/project/TGS_assembly/other_assemblers/clr/mhc/25x/falconunzip/3-un
zip/1-hasm
+ cd /export/scratch3/vincent/project/TGS_assembly/other_assemblers/clr/mhc/25x/falconunzip/3-
unzip/1-hasm
/bin/bash task.sh
+ /bin/bash task.sh
pypeflow 2.2.0
2020-03-14 22:10:24,164 - root - DEBUG - Running "/export/scratch3/vincent/software/miniconda3
/envs/pacbio/lib/python2.7/site-packages/pypeflow/do_task.py /export/scratch3/vincent/project/
TGS_assembly/other_assemblers/clr/mhc/25x/falconunzip/3-unzip/1-hasm/task.json"
2020-03-14 22:10:24,165 - root - DEBUG - Checking existence of '/export/scratch3/vincent/proje
ct/TGS_assembly/other_assemblers/clr/mhc/25x/falconunzip/3-unzip/1-hasm/task.json' with timeou
t=30
2020-03-14 22:10:24,165 - root - DEBUG - Loading JSON from '/export/scratch3/vincent/project/T
GS_assembly/other_assemblers/clr/mhc/25x/falconunzip/3-unzip/1-hasm/task.json'
2020-03-14 22:10:24,165 - root - DEBUG - {u'bash_template_fn': u'template.sh',
u'inputs': {u'las_fofn': u'../../1-preads_ovl/las-merge-combine/las_fofn.json',
u'preads4falcon': u'../../1-preads_ovl/db2falcon/preads4falcon.fasta',
u'preads_db': u'../../1-preads_ovl/build/preads.db',
u'rid_to_phase_all': u'concatenated-rid-to-phase/rid_to_phase.all'},
u'outputs': {u'p_ctg': u'p_ctg.fa'},
u'parameters': {u'pypeflow_mb': 4000,
u'pypeflow_nproc': u'48',
u'topdir': u'../..'}}
2020-03-14 22:10:24,166 - root - INFO - CD: '/export/scratch3/vincent/project/TGS_assembly/other_assemblers/clr/mhc/25x/falconunzip/3-unzip/1-hasm' <- '/export/scratch3/vincent/project/TGS_assembly/other_assemblers/clr/mhc/25x/falconunzip/3-unzip/1-hasm'
2020-03-14 22:10:24,166 - root - DEBUG - Checking existence of u'concatenated-rid-to-phase/rid_to_phase.all' with timeout=30
2020-03-14 22:10:24,166 - root - DEBUG - Checking existence of u'../../1-preads_ovl/build/preads.db' with timeout=30
2020-03-14 22:10:24,166 - root - DEBUG - Checking existence of u'../../1-preads_ovl/las-merge-combine/las_fofn.json' with timeout=30
2020-03-14 22:10:24,166 - root - DEBUG - Checking existence of u'../../1-preads_ovl/db2falcon/preads4falcon.fasta' with timeout=30
2020-03-14 22:10:24,166 - root - DEBUG - Checking existence of u'template.sh' with timeout=30
2020-03-14 22:10:24,166 - root - INFO - CD: '/export/scratch3/vincent/project/TGS_assembly/other_assemblers/clr/mhc/25x/falconunzip/3-unzip/1-hasm' <- '/export/scratch3/vincent/project/TGS_assembly/other_assemblers/clr/mhc/25x/falconunzip/3-unzip/1-hasm'
2020-03-14 22:10:24,189 - root - INFO - $('/bin/bash user_script.sh')
hostname
+ hostname
pwd
+ pwd
date
+ date
# Substitution will be similar to snakemake "shell".
# TODO: Needs preads.db
rm -f ./ctg_paths
+ rm -f ./ctg_paths
python -m falcon_unzip.mains.ovlp_filter_with_phase_strict --fofn ../../1-preads_ovl/las-merge-combine/las_fofn.json --max-diff 120 --max-cov 120 --min-cov 1 --n-core 48 --min-len 2500 --db ../../1-preads_ovl/build/preads.db --rid-phase-map concatenated-rid-to-phase/rid_to_phase.all > preads.p_ovl
+ python -m falcon_unzip.mains.ovlp_filter_with_phase_strict --fofn ../../1-preads_ovl/las-merge-combine/las_fofn.json --max-diff 120 --max-cov 120 --min-cov 1 --n-core 48 --min-len 2500 --db ../../1-preads_ovl/build/preads.db --rid-phase-map concatenated-rid-to-phase/rid_to_phase.all
falcon-unzip 1.2.0 (pip thinks "falcon-unzip 1.2.0")
falcon-kit 1.3.0
pypeflow 2.2.0
Traceback (most recent call last):
File "/export/scratch1/home/vincent/software/miniconda3/envs/pacbio/lib/python2.7/runpy.py", line 174, in _run_module_as_main
"__main__", fname, loader, pkg_name)
File "/export/scratch1/home/vincent/software/miniconda3/envs/pacbio/lib/python2.7/runpy.py", line 72, in _run_code
exec code in run_globals
File "/export/scratch3/vincent/software/miniconda3/envs/pacbio/lib/python2.7/site-packages/falcon_unzip/mains/ovlp_filter_with_phase_strict.py", line 408, in <module>
main()
File "/export/scratch3/vincent/software/miniconda3/envs/pacbio/lib/python2.7/site-packages/falcon_unzip/mains/ovlp_filter_with_phase_strict.py", line 404, in main
run(args)
File "/export/scratch3/vincent/software/miniconda3/envs/pacbio/lib/python2.7/site-packages/falcon_unzip/mains/ovlp_filter_with_phase_strict.py", line 333, in run
arid2phase[row[0]] = (row[1], row[2], row[3]) # ctg_id, phase_blk_id, phase_id
IndexError: list index out of range
2020-03-14 22:10:24,355 - root - WARNING - Call '/bin/bash user_script.sh' returned 256.
2020-03-14 22:10:24,355 - root - INFO - CD: '/export/scratch3/vincent/project/TGS_assembly/other_assemblers/clr/mhc/25x/falconunzip/3-unzip/1-hasm' -> '/export/scratch3/vincent/project/TGS_assembly/other_assemblers/clr/mhc/25x/falconunzip/3-unzip/1-hasm'
2020-03-14 22:10:24,355 - root - INFO - CD: '/export/scratch3/vincent/project/TGS_assembly/other_assemblers/clr/mhc/25x/falconunzip/3-unzip/1-hasm' -> '/export/scratch3/vincent/project/TGS_assembly/other_assemblers/clr/mhc/25x/falconunzip/3-unzip/1-hasm'
2020-03-14 22:10:24,356 - root - CRITICAL - Error in /export/scratch3/vincent/software/miniconda3/envs/pacbio/lib/python2.7/site-packages/pypeflow/do_task.py with args="{'json_fn': '/export/scratch3/vincent/project/TGS_assembly/other_assemblers/clr/mhc/25x/falconunzip/3-unzip/1-hasm/task.json',\n 'timeout': 30,\n 'tmpdir': None}"
Traceback (most recent call last):
File "/export/scratch1/home/vincent/software/miniconda3/envs/pacbio/lib/python2.7/runpy.py", line 174, in _run_module_as_main
"__main__", fname, loader, pkg_name)
File "/export/scratch1/home/vincent/software/miniconda3/envs/pacbio/lib/python2.7/runpy.py", line 72, in _run_code
exec code in run_globals
File "/export/scratch3/vincent/software/miniconda3/envs/pacbio/lib/python2.7/site-packages/pypeflow/do_task.py", line 281, in <module>
main()
File "/export/scratch3/vincent/software/miniconda3/envs/pacbio/lib/python2.7/site-packages/pypeflow/do_task.py", line 273, in main
run(**vars(parsed_args))
File "/export/scratch3/vincent/software/miniconda3/envs/pacbio/lib/python2.7/site-packages/pypeflow/do_task.py", line 267, in run
run_cfg_in_tmpdir(cfg, tmpdir, '.')
File "/export/scratch3/vincent/software/miniconda3/envs/pacbio/lib/python2.7/site-packages/pypeflow/do_task.py", line 242, in run_cfg_in_tmpdir
run_bash(bash_template, myinputs, myoutputs, parameters)
File "/export/scratch3/vincent/software/miniconda3/envs/pacbio/lib/python2.7/site-packages/pypeflow/do_task.py", line 201, in run_bash
util.system(cmd)
File "/export/scratch1/home/vincent/software/miniconda3/envs/pacbio/lib/python2.7/site-packages/pypeflow/io.py", line 29, in syscall
raise Exception(msg)
Exception: Call '/bin/bash user_script.sh' returned 256.
+++ pwd
++ echo 'FAILURE. Running top in /export/scratch3/vincent/project/TGS_assembly/other_assemblers/clr/mhc/25x/falconunzip/3-unzip/1-hasm (If you see -terminal database is inaccessible- you are using the python bin-wrapper, so you will not get diagnostic info. No big deal. This process is crashing anyway.)'
++ rm -f top.txt
++ which python
++ which top
++ env -u LD_LIBRARY_PATH top -b -n 1
++ env -u LD_LIBRARY_PATH top -b -n 1
++ pstree -apl
real 0m0.585s
user 0m0.296s
sys 0m0.141s
+ finish
+ echo 'finish code: 1'
The text was updated successfully, but these errors were encountered:
Sign up for freeto subscribe to this conversation on GitHub.
Already have an account?
Sign in.
HI, I run Falcon on pacbio reads successfully, but failed to run falcon-unzip. Here is the error report:
It seems that errors occurred at this step 3-unzip/1-hasm. The file 'preads.p_ovl' is empty. could you please help me to solve this issue? Many thanks.
The log information in file 'run-P5d87a9de2f785c.bash.stderr' under this folder '3-unzip/1-hasm'
The text was updated successfully, but these errors were encountered: