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Falcon Unzip crashed at 0-phasing (blasr ?) #81

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agroppi opened this issue May 31, 2017 · 6 comments
Open

Falcon Unzip crashed at 0-phasing (blasr ?) #81

agroppi opened this issue May 31, 2017 · 6 comments

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@agroppi
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agroppi commented May 31, 2017

I'm using the last version of falcon-unzip after an assembly with the lastest version of falcon.
I have blasr 5.3
python 2.7.12
The first step 3-unzip/reads ended
but at the 0-phasing step
Directories are created (here is an exemple : 0-phasing/000011F)
But after that the pipeline stops
Here is my cfg file :

[General]
job_type = local

[Unzip]
# list of fasta files
input_fofn = /mnt/cbib/sWAGMAN/Marouch/Falcon_0.5_Marouch/input_Marouch_falcon_0.5.fofn
# input_bam_fofn= input_bams.fofn

# path to bin directory containing samtools, blasr, and various GenomicConsensus utilities
smrt_bin=/module/apps/samtools/1.4/bin/ /module/apps/blasr/5.3/blasr/blasr /module/apps/python/2.7.12/bin/ /home/ag/GenomicConsensus/bin/


sge_phasing= --nodes 1 --ntasks-per-node 6
sge_quiver= --nodes 1 --ntasks-per-node 10
sge_track_reads= --nodes 1 --ntasks-per-node 6
sge_blasr_aln= --nodes 1 --ntasks-per-node 10
sge_hasm= --nodes 1 --ntasks-per-node 20
unzip_concurrent_jobs = 20
quiver_concurrent_jobs = 20

and here attached the all.log :
all.log.txt

@nottwy
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nottwy commented Sep 22, 2017

Can you check if there is a file called '000191F_reads.fa' under the directory 3-unzip/reads/ ?

@agroppi
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agroppi commented Sep 27, 2017

Hi
YES there is a file called 000191F_reads.fa under the directory 3-unzip/reads/

@nottwy
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nottwy commented Sep 28, 2017

You can attach your file '3-unzip/0-phasing/000191F/blasr/pwatcher/stderr' here.

@agroppi
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agroppi commented Sep 28, 2017

I don't have 3-unzip/0-phasing/000191F/blasr/pwatcher/stderr
In the directory 3-unzip there is only the directory reads

But in the directory reads I have a symbolic link to
mypwatcher/jobs/P4e06e95c523470/stderr

Here is this file :
stderr.zip

@nottwy
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nottwy commented Sep 28, 2017

You have to change the name of your fofn file into input.fofn.
Unzip only supports this name so fat. It didn't parse the name in your unzip.cfg.

@agroppi
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agroppi commented Sep 28, 2017

Thanks for your quick answer.
I've changed the names of my config and fofn files
I've just relaunched Falcon Unzip
I'll keep you informed

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