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Pigeon make-seurat produces empty output #727

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TheCatalyticMicroChip opened this issue Nov 11, 2024 · 0 comments
Open

Pigeon make-seurat produces empty output #727

TheCatalyticMicroChip opened this issue Nov 11, 2024 · 0 comments

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@TheCatalyticMicroChip
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Operating system
I am using Linux 3.10.0-1160.88.1.el7.x86_64

Package name
I am using loading StdEnv/2023 smrtlink-sequel2/13.1.0.221970 ... pigeon 1.2.0

Conda environment
Working on an HPC

Describe the bug
I am following the isoseq workflow as described here: https://isoseq.how/classification/workflow.html, but I believe the last command fails: pigeon make-seurat --dedup B_dedup.fasta --group B.collapsed.group.txt -d make-seurat B_classification.filtered_lite_classification.txt

Error message
I am not getting an error message, I am getting empty files in the last step of the workflow. info.csv has the expected information, however, annotated.info.csv and annotated-prefilter.info.csv are empty. See below the first two lines of input files:
Question
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