Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

why CLIPPER only generates rep1 and rep2? #42

Open
FionaMoon opened this issue Dec 4, 2023 · 2 comments
Open

why CLIPPER only generates rep1 and rep2? #42

FionaMoon opened this issue Dec 4, 2023 · 2 comments

Comments

@FionaMoon
Copy link

Hi eCLIP group,
I run eclip pipeline calls enriched peak regions (peak clusters) with CLIPPER.
I finally got bed file like this

image

Only 2 replicates in the output file.

I have 3 experiment data and 3 controls. I merged 3 experiment data with samtools and I expected to get 3 replicates in output files.

Could you give me some advise to solve this problem?
Thank you in advance.

@byee4
Copy link
Member

byee4 commented Dec 4, 2023

If more than one entry has the same name, final outputs from those entries might override each other. Check if that might be the case here

@FionaMoon
Copy link
Author

I've tried to rename these bam files and merged them as the input of Clipper.
I found no matter how many bam files I merged, the output of clipper would always have 2 replicates.

I also see another answer which said clipper can only dual with 2 replicates so it's better to use skipper.

Is that true? What should I do If I have multiple replicates and want to perform peak calling and normalization?

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

2 participants