BUSCO error

[distilledchild]

Hi,
I got this error even though I manually downloaded eukaryota database.
How could get BUSCO test result?

Thank you.

ERROR! Failed downloading eukaryota database (url: https://busco.ezlab.org/v2/datasets/eukaryota_odb9.tar.gz), QUAST functionality will be limited! Exception caught: HTTP Error 301: Moved Permanently
You can try to download the file manually, place it in /mnt/c/user/tools/envs/quast/lib/python3.7/site-packages/quast_libs/busco/eukaryota.tar.gz and restart QUAST
WARNING: Failed to download eukaryota database from https://busco.ezlab.org/v2/datasets/eukaryota_odb9.tar.gz and unpack it into /mnt/c/user/tools/envs/quast/lib/python3.7/site-packages/quast_libs/busco
Failed finding conservative genes.

[alexeigurevich]

Hi,
The url works for manual download, but QUAST fails to retrieve the file via Python libraries since the file is indeed moved permanently.
There are two possible workarounds:

1. Download the file manually (as you did already), move it to /mnt/c/user/tools/envs/quast/lib/python3.7/site-packages/quast_libs/busco/eukaryota.tar.gz (as suggested in the log), and restart QUAST. Mind that the moved file should be named exactly as suggested (eukaryota.tar.gz), NOT as the downloaded file (eukaryota_odb9.tar.gz). QUAST should skip the downloading step in this case: it will unpack the local file and proceed with it.
2. Try out our 5.1.0rc1 prerelease, where we fixed the BUSCO urls. We plan to release the stable version pretty soon.

Feel free to reopen the issue if you face any problems with any of these workarounds.