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diamond blastp is producing non-deterministic alignment output #844

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etvedte opened this issue Dec 18, 2024 · 1 comment
Open

diamond blastp is producing non-deterministic alignment output #844

etvedte opened this issue Dec 18, 2024 · 1 comment

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@etvedte
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etvedte commented Dec 18, 2024

Hello,

We've been testing diamond blastp v.2.1.8 and finding cases where the same inputs and parameters are producing non-deterministic alignment output. Could you take a look at some example files we have been working with?

https://ftp.ncbi.nlm.nih.gov/pub/murphyte/diamond_test/
example_1_cmd and example_1* output files show a small test case.
example_2_cmd and example_2* output files show larger test cases more similar to what we are working with in our pipeline.

Default parameters appear to be producing deterministic output with our queries/db. Of our added parameters, we believe specifying --masking 0 --very-sensitive together may be the culprit.

Thanks.

@bbuchfink
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I tracked down the problem, it will be fixed in the next release.

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