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installation issues #3318
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I think you probably have a samtools in your PATH that is superseding the one that bcbio installs. If you do |
no it should not as this is new ecs optimized instance and has only bcbio installed on it and it does gives bcbio installed samtools [ec2-user@ ~]$ which samtools |
I'm stumped. What does |
hmm, i did that (base) [ec2-user@ Even after adding the libarary path it does not recognize it and i do see the file in the lib folder. |
the library i have is (base) [ec2-user@ ~]$ ls -al /newvolume/bcbio/anaconda/lib/libcrypto.so.1.1 i guess its expecting to have libcrypto.so.1.0.0: |
Ok! I think Can you try:
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Hi, Thank you. the above solution worked. now i have another road block The above exception was the direct cause of the following exception: Traceback (most recent call last): |
I added this line to the above line in utils.py and it passed, but still have error from pool.py |
hi, smoove is complaining and when i try to install this is what it says conda install -c bioconda smoove UnsatisfiableError: The following specifications were found Specifications:
Your python: python=3.6 If python is on the left-most side of the chain, that's the version you've asked for. |
Hi @DiyaVaka ! What is the original smoove error? Why would you need to reinstall it? Can you show what is in your PATH? Sergey |
/newvolume/bcbio/anaconda/bin:/newvolume/bcbio/anaconda/condabin:/usr/local/bin:/usr/bin:/usr/local/sbin:/usr/sbin:/home/ec2-user/.local/bin:/home/ec2-user/bin |
the original error with smoove was it could not find it and there is not one available |
Hi! Your installation seems to be broken. What was your installation command? A successful installation should create two bin directories:
You need to put both dirs in your PATH. smoove goes into tools:
Sergey |
I see to be hitting a similar issue with 1.2.9 clean installed on a new EC2 instance running Ubuntu 20:
|
Looks like there's a problem with /opt/koopa/opt/bcbio-nextgen/install/anaconda/bin/samtools
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Here's the current list of environments: /opt/koopa/opt/bcbio-nextgen/install/anaconda/condabin/conda env list
@naumenko-sa Does CloudBioLinux need an update? I can work on debugging this. These seems related: |
Which samtools version did you end up having in your main bcbio env? I've been seeing this problem recently as well: #3557 |
@amizeranschi I'm working on debugging this! Will let you know Posting in your other thread. Let's keep this closed. |
Hi,
I have a clean install of bcbio and everything went fine with installation and no errors. While I tried to run sample it gave the following errors
Installed version of samtools sort does not have support for multithreading (-@ option) required to support bwa piped alignment and BAM merging. Please upgrade to the latest version from http://samtools.sourceforge.net/
Then i tried to do
but still getting the same error message.
I tried to do conda install samtools tools but it says everything is up to date
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