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Watchout for alignments with > 65535 CIGAR operations #59

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SHuang-Broad opened this issue Jan 17, 2020 · 3 comments
Closed

Watchout for alignments with > 65535 CIGAR operations #59

SHuang-Broad opened this issue Jan 17, 2020 · 3 comments

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@SHuang-Broad
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As the reads and assemblies gets longer, we will get there.
In fact we might already be there for some assemblies with NG50 > 10Mb.

See minimap2's comment about how to do that.

A tricky thing is how up-to-date downstream tools are, when it comes to adhering to the hts-specs.

@SHuang-Broad
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The alignment workflow now turns on -L by default.
We are unsure if the downstream tools are aware of the CG tag yet.

@kvg
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kvg commented Feb 20, 2020

I may have broken this when the merge of the kvg_ont_fixes branch into master. We should update the AlignReads.Minimap2 task.

@kvg
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kvg commented Apr 1, 2020

Done - by default we now supply the -L argument to minimap2, which writes long cigar strings to a special tag.

@kvg kvg closed this as completed Apr 1, 2020
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