Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

How to resume #154

Open
methylome opened this issue Sep 26, 2022 · 0 comments
Open

How to resume #154

methylome opened this issue Sep 26, 2022 · 0 comments

Comments

@methylome
Copy link

pilon was failed with the following log. Is there any way to resume the job?

Pilon version 1.22 Wed Mar 15 16:38:30 2017 -0400
Genome: /ddn/datacenter/genomes/Polymesoda_erosa/assembly/flye/racon/assembly_racon.fa
Fixing snps, indels, gaps, local
Input genome size: 2359242266
Scanning BAMs
/ddn/datacenter/genomes/Polymesoda_erosa/assembly/flye/pilon/assembly_racon.bam: 530334753 reads, 0 filtered, 529476652 mapped, 509061207 proper, 18527354 stray, FR 100% 496+/-121, max 1548
Processing contig_61897:1-52237
...
# fix break: contig_63515:26157-26251 0 -0 +0 NoSolution
# fix break: contig_63515:26636 0 -0 +0 NoSolution
Finished processing contig_63515:1-28000
Processing contig_18993:1-49004
WARNING: BAM index file /ddn/datacenter/genomes/Polymesoda_erosa/assembly/flye/pilon/assembly_racon.bam.bai is older than BAM /ddn/datacenter/genomes/Polymesoda_erosa/assembly/flye/pilon/assembly_racon.bam
WARNING: BAM index file /ddn/datacenter/genomes/Polymesoda_erosa/assembly/flye/pilon/assembly_racon.bam.bai is older than BAM /ddn/datacenter/genomes/Polymesoda_erosa/assembly/flye/pilon/assembly_racon.bam
WARNING: BAM index file /ddn/datacenter/genomes/Polymesoda_erosa/assembly/flye/pilon/assembly_racon.bam.bai is older than BAM /ddn/datacenter/genomes/Polymesoda_erosa/assembly/flye/pilon/assembly_racon.bam
WARNING: BAM index file /ddn/datacenter/genomes/Polymesoda_erosa/assembly/flye/pilon/assembly_racon.bam.bai is older than BAM /ddn/datacenter/genomes/Polymesoda_erosa/assembly/flye/pilon/assembly_racon.bam
WARNING: BAM index file /ddn/datacenter/genomes/Polymesoda_erosa/assembly/flye/pilon/assembly_racon.bam.bai is older than BAM /ddn/datacenter/genomes/Polymesoda_erosa/assembly/flye/pilon/assembly_racon.bam
Exception in thread "main" java.lang.reflect.InvocationTargetException
   at sun.reflect.NativeMethodAccessorImpl.invoke0(Native Method)
   at sun.reflect.NativeMethodAccessorImpl.invoke(NativeMethodAccessorImpl.java:62)
   at sun.reflect.DelegatingMethodAccessorImpl.invoke(DelegatingMethodAccessorImpl.java:43)
   at java.lang.reflect.Method.invoke(Method.java:498)
   at com.simontuffs.onejar.Boot.run(Boot.java:340)
   at com.simontuffs.onejar.Boot.main(Boot.java:166)
Caused by: htsjdk.samtools.util.RuntimeIOException: /ddn/datacenter/genomes/Polymesoda_erosa/assembly/flye/pilon/assembly_racon.bam.bai (Cannot send after transport endpoint shutdown)
   at htsjdk.samtools.AbstractBAMFileIndex$MemoryMappedFileBuffer.<init>(AbstractBAMFileIndex.java:478)
   at htsjdk.samtools.AbstractBAMFileIndex.<init>(AbstractBAMFileIndex.java:79)
   at htsjdk.samtools.DiskBasedBAMFileIndex.<init>(DiskBasedBAMFileIndex.java:46)
   at htsjdk.samtools.BAMFileReader.getIndex(BAMFileReader.java:252)
   at htsjdk.samtools.BAMFileReader.createIndexIterator(BAMFileReader.java:726)
   at htsjdk.samtools.BAMFileReader.query(BAMFileReader.java:412)
   at htsjdk.samtools.SamReader$PrimitiveSamReaderToSamReaderAdapter.query(SamReader.java:498)
   at htsjdk.samtools.SamReader$PrimitiveSamReaderToSamReaderAdapter.query(SamReader.java:503)
   at htsjdk.samtools.SamReader$PrimitiveSamReaderToSamReaderAdapter.queryOverlapping(SamReader.java:365)
   at org.broadinstitute.pilon.BamFile.readsInRegion(BamFile.scala:329)
   at org.broadinstitute.pilon.BamFile.recruitFlankReads(BamFile.scala:336)
   at org.broadinstitute.pilon.GapFiller$$anonfun$recruitReadsOfType$1.apply(GapFiller.scala:367)
   at org.broadinstitute.pilon.GapFiller$$anonfun$recruitReadsOfType$1.apply(GapFiller.scala:366)
   at scala.collection.immutable.List.foreach(List.scala:381)
   at org.broadinstitute.pilon.GapFiller.recruitReadsOfType(GapFiller.scala:366)
   at org.broadinstitute.pilon.GapFiller.recruitFrags(GapFiller.scala:375)
   at org.broadinstitute.pilon.GapFiller.recruitLocalReads(GapFiller.scala:389)
   at org.broadinstitute.pilon.GapFiller.recruitReads(GapFiller.scala:391)
   at org.broadinstitute.pilon.GapFiller.assembleAcrossBreak(GapFiller.scala:51)
   at org.broadinstitute.pilon.GapFiller.fixBreak(GapFiller.scala:45)
   at org.broadinstitute.pilon.GenomeRegion$$anonfun$identifyAndFixIssues$4.apply(GenomeRegion.scala:383)
   at org.broadinstitute.pilon.GenomeRegion$$anonfun$identifyAndFixIssues$4.apply(GenomeRegion.scala:381)
   at scala.collection.immutable.List.foreach(List.scala:381)
   at org.broadinstitute.pilon.GenomeRegion.identifyAndFixIssues(GenomeRegion.scala:381)
   at org.broadinstitute.pilon.GenomeFile$$anonfun$processRegions$4.apply(GenomeFile.scala:119)
   at org.broadinstitute.pilon.GenomeFile$$anonfun$processRegions$4.apply(GenomeFile.scala:108)
   at scala.collection.Iterator$class.foreach(Iterator.scala:893)
   at scala.collection.AbstractIterator.foreach(Iterator.scala:1336)
   at scala.collection.parallel.ParIterableLike$Foreach.leaf(ParIterableLike.scala:972)
   at scala.collection.parallel.Task$$anonfun$tryLeaf$1.apply$mcV$sp(Tasks.scala:49)
   at scala.collection.parallel.Task$$anonfun$tryLeaf$1.apply(Tasks.scala:48)
   at scala.collection.parallel.Task$$anonfun$tryLeaf$1.apply(Tasks.scala:48)
   at scala.collection.parallel.Task$class.tryLeaf(Tasks.scala:51)
   at scala.collection.parallel.ParIterableLike$Foreach.tryLeaf(ParIterableLike.scala:969)
   at scala.collection.parallel.AdaptiveWorkStealingTasks$WrappedTask$class.internal(Tasks.scala:169)
   at scala.collection.parallel.AdaptiveWorkStealingForkJoinTasks$WrappedTask.internal(Tasks.scala:443)
   at scala.collection.parallel.AdaptiveWorkStealingTasks$WrappedTask$class.compute(Tasks.scala:149)
   at scala.collection.parallel.AdaptiveWorkStealingForkJoinTasks$WrappedTask.compute(Tasks.scala:443)
   at scala.concurrent.forkjoin.RecursiveAction.exec(RecursiveAction.java:160)
   at scala.concurrent.forkjoin.ForkJoinTask.doExec(ForkJoinTask.java:260)
   at scala.concurrent.forkjoin.ForkJoinPool$WorkQueue.runTask(ForkJoinPool.java:1339)
   at scala.concurrent.forkjoin.ForkJoinPool.runWorker(ForkJoinPool.java:1979)
   at scala.concurrent.forkjoin.ForkJoinWorkerThread.run(ForkJoinWorkerThread.java:107)
Caused by: java.io.FileNotFoundException: /ddn/datacenter/genomes/Polymesoda_erosa/assembly/flye/pilon/assembly_racon.bam.bai (Cannot send after transport endpoint shutdown)
   at java.io.FileInputStream.open0(Native Method)
   at java.io.FileInputStream.open(FileInputStream.java:195)
   at java.io.FileInputStream.<init>(FileInputStream.java:138)
   at htsjdk.samtools.AbstractBAMFileIndex$MemoryMappedFileBuffer.<init>(AbstractBAMFileIndex.java:471)
   ... 42 more
Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

1 participant