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Using longest ligation product can overestimate observed fraction #37

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cerebis opened this issue Jul 13, 2020 · 0 comments
Open

Using longest ligation product can overestimate observed fraction #37

cerebis opened this issue Jul 13, 2020 · 0 comments
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enhancement New feature or request

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@cerebis
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cerebis commented Jul 13, 2020

For multi-enzyme digests which produce varying length ligation products, use of the longest in estimating the observed fraction will lead to overestimation.

A more accurate value could be calculated as a weighted sum over the observed abundance of each possible junction. This information is already collected.
@cerebis cerebis added the enhancement New feature or request label Jul 13, 2020
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