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paired_end.sh
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#!/bin/sh
# output directories
html_dir=html
dot_dir=dot
txt_dir=txt
if [[ ! -d $html_dir ]]; then mkdir -v $html_dir; fi
if [[ ! -d $dot_dir ]]; then mkdir -v $dot_dir; fi
if [[ ! -d $txt_dir ]]; then mkdir -v $txt_dir; fi
# fastq screen configuration file
FSCREEN=conf/fastq_screen.conf
# multiqc configuration file (not mandatory)
MULTIQC_FILE=yml/multiqc_config.yml
nextflow run main.nf \
--design "csv/paired_end.csv" \
--output_directory "nextflow_paired_end" \
--binomial_nomenclature "homo sapiens" \
--strandedness "none" \
--read_length "77" \
--modules "yml/modules.yml" \
--genome_type "ensembl" \
--genome_version "GRCh38" \
--genome_release "86" \
--genome_sequence_extension "primary_assembly.fa" \
--fastq_screen_conf $FSCREEN \
--publish_directory_mode "symlink" \
--publish_directory_overwrite \
--r_script "r/analysis_example.r" \
--multiqc_config $MULTIQC_FILE \
-with-timeline $html_dir/timeline_paired_end.html \
-with-dag $dot_dir/dag_paired_end.dot \
-with-trace $txt_dir/trace_paired_end.txt \
-resume