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Question about low coverage bins #1

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skelviper opened this issue Sep 8, 2021 · 0 comments
Open

Question about low coverage bins #1

skelviper opened this issue Sep 8, 2021 · 0 comments

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@skelviper
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HI! After read your paper I notice in your Chromosome capture data processing sction you wrote
"Next, pairs were filtered using mapping quality scores (MAPQ > 30) on each side of aligned chimeric reads, binned into multiple resolutions, and low-coverage bins were removed"
Could you please tell me the defination of "low-coverage bins" and how you remove it? Is this step necessary before balancing? Is it in the cooler balance xxx.cool command? And a more general question is do you think remove ENCODE blacklist or other simmilar "remove bins" process necessary?
Thank you for your help!

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