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HI! After read your paper I notice in your Chromosome capture data processing sction you wrote
"Next, pairs were filtered using mapping quality scores (MAPQ > 30) on each side of aligned chimeric reads, binned into multiple resolutions, and low-coverage bins were removed"
Could you please tell me the defination of "low-coverage bins" and how you remove it? Is this step necessary before balancing? Is it in the cooler balance xxx.cool command? And a more general question is do you think remove ENCODE blacklist or other simmilar "remove bins" process necessary?
Thank you for your help!
The text was updated successfully, but these errors were encountered:
HI! After read your paper I notice in your Chromosome capture data processing sction you wrote
"Next, pairs were filtered using mapping quality scores (MAPQ > 30) on each side of aligned chimeric reads, binned into multiple resolutions, and low-coverage bins were removed"
Could you please tell me the defination of "low-coverage bins" and how you remove it? Is this step necessary before balancing? Is it in the cooler balance xxx.cool command? And a more general question is do you think remove ENCODE blacklist or other simmilar "remove bins" process necessary?
Thank you for your help!
The text was updated successfully, but these errors were encountered: