eco_from_obo.json

{
    "ECO:0000000": {
        "def": [
            "\"A type of information that is used to support an assertion.\" [ECO:MCC]"
        ],
        "disjoint_from": [
            "ECO:0000217 ! assertion method"
        ],
        "id": [
            "ECO:0000000"
        ],
        "is_a": [],
        "name": [
            "evidence"
        ],
        "synonym": [
            "\"evidence code\" RELATED []",
            "\"evidence_code\" RELATED []"
        ]
    },
    "ECO:0000001": {
        "def": [
            "\"A curator inference that links the current annotation to a different evidence-based annotation via background knowledge of the curator.\" [ECO:go, ECO:MCC]"
        ],
        "id": [
            "ECO:0000001"
        ],
        "is_a": [
            "ECO:0000205 ! curator inference"
        ],
        "name": [
            "inference from background scientific knowledge"
        ],
        "synonym": [
            "\"IC\" EXACT [GO:IC]",
            "\"inferred by curator\" EXACT [GO:IC]"
        ]
    },
    "ECO:0000002": {
        "def": [
            "\"Experimental evidence that is based on direct measurement of some aspect of a biological feature.\" [ECO:MCC, GO:IDA]"
        ],
        "id": [
            "ECO:0000002"
        ],
        "is_a": [
            "ECO:0000006 ! experimental evidence"
        ],
        "name": [
            "direct assay evidence"
        ]
    },
    "ECO:0000003": {
        "def": [
            "\"A type of direct assay evidence based on reconstructing a biological sample from its disassociated state to its original state.\" [ECO:KAV, PMID:26029343]"
        ],
        "id": [
            "ECO:0000003"
        ],
        "is_a": [
            "ECO:0000002 ! direct assay evidence"
        ],
        "name": [
            "reconstitution assay evidence"
        ]
    },
    "ECO:0000004": {
        "comment": [
            "If using this term for Gene Ontology annotation, it would be used most typically for annotations to the cellular component ontology."
        ],
        "def": [
            "\"A type of fractionation evidence where sub-cellular components are separated based on their physical properties such as density in a sucrose density gradient.\" [ECO:KIM, TAIR:TED]"
        ],
        "id": [
            "ECO:0000004"
        ],
        "is_a": [
            "ECO:0000100 ! fractionation evidence"
        ],
        "name": [
            "cell fractionation evidence"
        ],
        "synonym": [
            "\"IDA: cell fractionation\" RELATED []"
        ]
    },
    "ECO:0000005": {
        "def": [
            "\"Used when an annotation is made based on assays that determine the catalytic activity of enzymes.\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000005"
        ],
        "is_a": [
            "ECO:0000039 ! protein assay evidence"
        ],
        "name": [
            "enzyme assay evidence"
        ],
        "synonym": [
            "\"IDA: enzyme assays\" RELATED []"
        ],
        "xref": [
            "PSI-MI:MI\\:0415 \"enzymatic study\""
        ]
    },
    "ECO:0000006": {
        "def": [
            "\"An evidence type that is based on the results of a laboratory assay.\" [ECO:MCC, GOC:ecd]"
        ],
        "id": [
            "ECO:0000006"
        ],
        "is_a": [
            "ECO:0000000 ! evidence"
        ],
        "name": [
            "experimental evidence"
        ],
        "subset": [
            "go_groupings"
        ],
        "synonym": [
            "\"EXP\" EXACT [GO:EXP]",
            "\"inferred from experiment\" EXACT [GO:EXP]"
        ]
    },
    "ECO:0000007": {
        "def": [
            "\"A type of protein detection assay evidence where a fluorescently labeled antibody is used to detect the presence or localization of a biomolecule within a cell.\" [ECO:MCC, TAIR:TED]"
        ],
        "id": [
            "ECO:0000007"
        ],
        "is_a": [
            "ECO:0005517 ! protein detection assay evidence"
        ],
        "name": [
            "immunofluorescence evidence"
        ],
        "synonym": [
            "\"IDA: immunofluorescence\" RELATED []"
        ]
    },
    "ECO:0000008": {
        "comment": [
            "Use this evidence type when the annotation is inferred from the timing or location of expression of a gene. It may be difficult to determine whether the expression pattern truly indicates that a gene plays a role in a given process."
        ],
        "def": [
            "\"Experimental evidence that is based on characterization of gene expression.\" [ECO:MCC, GO:IEP]"
        ],
        "id": [
            "ECO:0000008"
        ],
        "is_a": [
            "ECO:0000006 ! experimental evidence"
        ],
        "name": [
            "expression pattern evidence"
        ],
        "synonym": [
            "\"IEP\" EXACT [GO:IEP]",
            "\"inferred from expression pattern\" EXACT [GO:IEP]"
        ]
    },
    "ECO:0000009": {
        "id": [
            "ECO:0000009"
        ],
        "is_a": [
            "ECO:0000008 ! expression pattern evidence"
        ],
        "name": [
            "transcript expression evidence"
        ]
    },
    "ECO:0000010": {
        "id": [
            "ECO:0000010"
        ],
        "is_a": [
            "ECO:0000008 ! expression pattern evidence",
            "ECO:0000039 ! protein assay evidence"
        ],
        "name": [
            "protein expression evidence"
        ]
    },
    "ECO:0000011": {
        "id": [
            "ECO:0000011"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "genetic interaction evidence"
        ],
        "xref": [
            "TAIR:TED\\:0000006"
        ]
    },
    "ECO:0000012": {
        "def": [
            "\"Used when an annotation is made based on a functional complementation assay in which a wild-type copy of the gene in question is inserted into a mutant background in the organism of the gene's origin or a heterologous organism with a mutation in the homologous gene.\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000012"
        ],
        "is_a": [
            "ECO:0000011 ! genetic interaction evidence"
        ],
        "name": [
            "functional complementation evidence"
        ],
        "synonym": [
            "\"IGI: functional complementation\" RELATED []"
        ]
    },
    "ECO:0000013": {
        "id": [
            "ECO:0000013"
        ],
        "is_a": [
            "ECO:0000012 ! functional complementation evidence"
        ],
        "name": [
            "transgenic rescue experiment evidence"
        ]
    },
    "ECO:0000015": {
        "def": [
            "\"The IMP evidence code covers those cases when the function, process or cellular localization of a gene product is inferred based on differences in the function, process, or cellular localization between two different alleles of the corresponding gene. The IMP code is used for cases where one allele may be designated 'wild-type' and another as 'mutant'. It is also used in cases where allelic variation occurs naturally and no specific allele is designated as wild-type or mutant. Caution should be used when making annotations from gain-of-function mutations as it may be difficult to infer a gene's normal function from a gain of function mutation, although it is sometimes possible.\" [GO:IMP]"
        ],
        "id": [
            "ECO:0000015"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "mutant phenotype evidence"
        ]
    },
    "ECO:0000016": {
        "id": [
            "ECO:0000016"
        ],
        "is_a": [
            "ECO:0000015 ! mutant phenotype evidence"
        ],
        "name": [
            "loss-of-function mutant phenotype evidence"
        ]
    },
    "ECO:0000017": {
        "def": [
            "\"Used when an annotation is made based on the analysis of the phenotype of a wild-type or mutant transgenic organism that has been engineered to overexpress or ectopically express the gene product in question.\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000017"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "ectopic expression evidence"
        ],
        "synonym": [
            "\"IMP: analysis of overexpression/ectopic expression phenotype\" RELATED []"
        ]
    },
    "ECO:0000018": {
        "def": [
            "\"Used when an annotation is based on a phenotype observed when expressing an anti-sense version of a gene product in a wild-type (for that gene product) background.\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000018"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "anti-sense experiment evidence"
        ],
        "synonym": [
            "\"IMP: anti-sense experiments\" RELATED []"
        ]
    },
    "ECO:0000019": {
        "def": [
            "\"Inference based on a phenotype observed when expressing an RNAi construct that includes a fragment of the gene product in a wild-type (for that gene product) background. A double-stranded RNA specifically suppresses the expression of the gene bearing its complementary sequence.\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000019"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "RNAi evidence"
        ],
        "synonym": [
            "\"IMP: RNAi experiment\" RELATED []"
        ]
    },
    "ECO:0000020": {
        "def": [
            "\"A type of protein assay evidence based on the inhibition of the molecular function of a protein.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000020"
        ],
        "is_a": [
            "ECO:0000039 ! protein assay evidence"
        ],
        "name": [
            "specific protein inhibition evidence"
        ]
    },
    "ECO:0000021": {
        "comment": [
            "Molecules interacted with might include protein, nucleic acid, ion, or complex."
        ],
        "def": [
            "\"Experimental evidence that is based on characterization of an interaction between a gene product and another molecule.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000021"
        ],
        "is_a": [
            "ECO:0000006 ! experimental evidence"
        ],
        "name": [
            "physical interaction evidence"
        ],
        "xref": [
            "PSI-MI:MI\\:0045 \"experimental interaction detection\""
        ]
    },
    "ECO:0000022": {
        "def": [
            "\"A type of physical interaction evidence where a cellular component subunit is isolated as part of purification of its larger complex.\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000022"
        ],
        "is_a": [
            "ECO:0000021 ! physical interaction evidence"
        ],
        "name": [
            "co-purification evidence"
        ],
        "synonym": [
            "\"IPI: co-purification\" RELATED []"
        ],
        "xref": [
            "PSI-MI:MI\\:0025 \"copurification\""
        ]
    },
    "ECO:0000023": {
        "def": [
            "\"A type of physical interaction evidence that depends on the strength of the interaction between two entities.\" [ECO:MCC, PSI-MI:MI\\:0400]"
        ],
        "id": [
            "ECO:0000023"
        ],
        "is_a": [
            "ECO:0000021 ! physical interaction evidence"
        ],
        "name": [
            "affinity evidence"
        ],
        "synonym": [
            "\"ligand binding evidence\" NARROW []"
        ],
        "xref": [
            "PSI-MI:MI\\:0400 \"affinity technology\""
        ]
    },
    "ECO:0000024": {
        "id": [
            "ECO:0000024"
        ],
        "is_a": [
            "ECO:0000023 ! affinity evidence",
            "ECO:0000039 ! protein assay evidence"
        ],
        "name": [
            "protein binding evidence"
        ]
    },
    "ECO:0000025": {
        "comment": [
            "Typically enzymes which confer resistance to antibiotics, such as Dihydrofolate reductase or Beta-lactamase, or proteins that give colorimetric or fluorescent signals are used. The Bait protein is generally the protein under study and the methods are readily adaptable to highthroughput mode."
        ],
        "def": [
            "\"A type of affinity evidence where proteins of interest (bait and prey) are covalently linked to incomplete fragments of a third protein (reporter) and expressed in vivo, at which time interaction between bait and prey proteins brings reporter fragments in close enough proximity to allow them to reform and become a functional reporter protein.\" [ECO:MCC, PSI-MI:MI\\:0090]"
        ],
        "id": [
            "ECO:0000025"
        ],
        "is_a": [
            "ECO:0000023 ! affinity evidence"
        ],
        "name": [
            "hybrid interaction evidence"
        ],
        "xref": [
            "PSI-MI:MI\\:0090 \"protein complementation assay\""
        ]
    },
    "ECO:0000026": {
        "id": [
            "ECO:0000026"
        ],
        "is_a": [
            "ECO:0000073 ! experimental genomic evidence"
        ],
        "name": [
            "nucleic acid hybridization evidence"
        ]
    },
    "ECO:0000027": {
        "comment": [
            "For GO annotation, in the case of a single gene, an accession for the related gene's sequence is entered in the evidence_with field."
        ],
        "def": [
            "\"A type of similarity evidence based on structural similarity of an annotated gene or gene product to another gene or group of genes.\" [ECO:MCC, TAIR:TED]"
        ],
        "id": [
            "ECO:0000027"
        ],
        "is_a": [
            "ECO:0000057 ! phenotypic similarity evidence"
        ],
        "name": [
            "structural similarity evidence"
        ]
    },
    "ECO:0000028": {
        "def": [
            "\"Used when an annotation is made based on the presence of a recognized domain or motif in a gene product's (usually protein) primary sequence.\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000028"
        ],
        "is_a": [
            "ECO:0000202 ! match to sequence model evidence"
        ],
        "name": [
            "motif similarity evidence"
        ],
        "synonym": [
            "\"ISS: recognized domains\" RELATED []"
        ]
    },
    "ECO:0000029": {
        "id": [
            "ECO:0000029"
        ],
        "is_a": [
            "ECO:0000202 ! match to sequence model evidence"
        ],
        "name": [
            "match to InterPro member signature evidence"
        ]
    },
    "ECO:0000030": {
        "def": [
            "\"A type of BLAST evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000030"
        ],
        "intersection_of": [
            "ECO:0000206 ! BLAST evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000206 ! BLAST evidence",
            "ECO:0005560 {is_inferred=\"true\"} ! pairwise sequence alignment evidence used in manual assertion"
        ],
        "name": [
            "BLAST evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ],
        "synonym": [
            "\"curated BLAST analysis\" RELATED []"
        ]
    },
    "ECO:0000031": {
        "def": [
            "\"Protein BLAST evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000031"
        ],
        "intersection_of": [
            "ECO:0000208 ! protein BLAST evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000030 {is_inferred=\"true\"} ! BLAST evidence used in manual assertion",
            "ECO:0000208 ! protein BLAST evidence"
        ],
        "name": [
            "protein BLAST evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ],
        "synonym": [
            "\"curated protein BLAST analysis\" RELATED []"
        ],
        "xref": [
            "GO_REF:0000012 \"Pairwise alignment (TIGR)\"",
            "GO_REF:0000027 \"BLAST search criteria for ISS assignment in PAMGO_GAT\""
        ]
    },
    "ECO:0000032": {
        "def": [
            "\"Nucleotide BLAST evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000032"
        ],
        "intersection_of": [
            "ECO:0000207 ! nucleotide BLAST evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000030 {is_inferred=\"true\"} ! BLAST evidence used in manual assertion",
            "ECO:0000207 ! nucleotide BLAST evidence"
        ],
        "name": [
            "nucleotide BLAST evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ],
        "synonym": [
            "\"curated nucleic acid BLAST analysis\" RELATED []"
        ]
    },
    "ECO:0000033": {
        "comment": [
            "The traceable author statement (TAS) evidence code covers author statements that are attributed to a cited source. Typically this type of information comes from review articles. Material from the introductions and discussion sections of non-review papers may also be suitable if another reference is cited as the source of experimental work or analysis. When annotating with this code the curator should use caution and be aware that authors often cite papers dealing with experiments that were performed in organisms different from the one being discussed in the paper at hand. Thus a problem with the TAS code is that it may turn out from following up the references in the paper that no experiments were performed on the gene in the organism actually being characterized in the primary paper. For this reason we recommend (when time and resources allow) that curators track down the cited paper and annotate directly from the experimental paper using the appropriate experimental evidence code. When this is not possible and it is necessary to annotate from reviews, the TAS code is the appropriate code to use for statements that are associated with a cited reference. Once an annotation has been made to a given term using an experimental evidence code, we recommend removing any annotations made to the same term using the TAS evidence code."
        ],
        "def": [
            "\"An author statement that is based on a cited reference.\" [ECO:MCC, GO:TAS]"
        ],
        "disjoint_from": [
            "ECO:0000034 ! non-traceable author statement"
        ],
        "id": [
            "ECO:0000033"
        ],
        "is_a": [
            "ECO:0000204 ! author statement"
        ],
        "name": [
            "traceable author statement"
        ]
    },
    "ECO:0000034": {
        "comment": [
            "The non-traceable author statement evidence code should be used in all cases where the author makes a statement that a curator wants to capture but for which there are neither results presented nor a specific reference cited in the source used to make the annotation. The source of the information may be peer reviewed papers, textbooks, database records or vouchered specimens."
        ],
        "def": [
            "\"An author statement that is not associated with results presented or a cited reference.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000034"
        ],
        "is_a": [
            "ECO:0000204 ! author statement"
        ],
        "name": [
            "non-traceable author statement"
        ]
    },
    "ECO:0000035": {
        "comment": [
            "An assertion of \"no biological data found\" carries the assumption that a more-or-less exhaustive search has been conducted."
        ],
        "def": [
            "\"A curator inference that results from research where no information about a biological feature was found in the scientific literature, at biological databases, or within other resources.\" [ECO:MCC, GO:ND]"
        ],
        "id": [
            "ECO:0000035"
        ],
        "is_a": [
            "ECO:0000205 ! curator inference"
        ],
        "name": [
            "no biological data found"
        ],
        "synonym": [
            "\"ND\" EXACT [GO:ND]",
            "\"no biological data available\" EXACT [GO:ND]"
        ]
    },
    "ECO:0000037": {
        "comment": [
            "The evidence not_recorded appears in some legacy annotations; it should not be used for new annotations."
        ],
        "def": [
            "\"An evidence that reflects an annotation was made before curators began tracking evidence types.\" [ECO:MCC, GO:NR]"
        ],
        "id": [
            "ECO:0000037"
        ],
        "is_a": [],
        "is_obsolete": [
            "true"
        ],
        "name": [
            "not_recorded"
        ],
        "synonym": [
            "\"not recorded\" EXACT [GO:NR]",
            "\"NR\" EXACT [GO:NR]"
        ]
    },
    "ECO:0000038": {
        "id": [
            "ECO:0000038"
        ],
        "is_a": [
            "ECO:0000012 ! functional complementation evidence"
        ],
        "name": [
            "transient rescue experiment evidence"
        ]
    },
    "ECO:0000039": {
        "id": [
            "ECO:0000039"
        ],
        "is_a": [
            "ECO:0000002 ! direct assay evidence"
        ],
        "name": [
            "protein assay evidence"
        ]
    },
    "ECO:0000040": {
        "id": [
            "ECO:0000040"
        ],
        "is_a": [
            "ECO:0000023 ! affinity evidence"
        ],
        "name": [
            "immunological assay evidence"
        ]
    },
    "ECO:0000041": {
        "def": [
            "\"An evidence type that is based on comparing likeness of distinct biological entities.\" [ECO:MCC, PhenoScape:IS]"
        ],
        "id": [
            "ECO:0000041"
        ],
        "is_a": [
            "ECO:0000000 ! evidence"
        ],
        "name": [
            "similarity evidence"
        ],
        "synonym": [
            "\"inferred from similarity\" RELATED []",
            "\"IS\" EXACT []"
        ]
    },
    "ECO:0000042": {
        "id": [
            "ECO:0000042"
        ],
        "is_a": [
            "ECO:0000015 ! mutant phenotype evidence"
        ],
        "name": [
            "gain-of-function mutant phenotype evidence"
        ]
    },
    "ECO:0000044": {
        "comment": [
            "A sequence similarity analysis may involve a gene or a gene product, and it could be based on similarity to a single other gene or to a group of other genes."
        ],
        "def": [
            "\"A type of similarity based on biomolecular sequence.\" [ECO:MCC, TAIR:TED]"
        ],
        "id": [
            "ECO:0000044"
        ],
        "is_a": [
            "ECO:0000041 ! similarity evidence"
        ],
        "name": [
            "sequence similarity evidence"
        ],
        "synonym": [
            "\"inferred from sequence or structural similarity\" BROAD [GO:ISS]",
            "\"inferred from sequence similarity\" EXACT []",
            "\"ISS\" BROAD [GO:ISS]"
        ]
    },
    "ECO:0000045": {
        "id": [
            "ECO:0000045"
        ],
        "is_a": [
            "ECO:0000010 ! protein expression evidence"
        ],
        "name": [
            "spatial pattern of protein expression evidence"
        ]
    },
    "ECO:0000046": {
        "id": [
            "ECO:0000046"
        ],
        "is_a": [
            "ECO:0000010 ! protein expression evidence"
        ],
        "name": [
            "protein expression level evidence"
        ]
    },
    "ECO:0000047": {
        "id": [
            "ECO:0000047"
        ],
        "is_a": [
            "ECO:0000009 ! transcript expression evidence"
        ],
        "name": [
            "spatial pattern of transcript expression evidence"
        ]
    },
    "ECO:0000048": {
        "id": [
            "ECO:0000048"
        ],
        "is_a": [
            "ECO:0000009 ! transcript expression evidence"
        ],
        "name": [
            "transcript expression level evidence"
        ]
    },
    "ECO:0000049": {
        "def": [
            "\"Used when an annotation is made on the basis of the expression pattern of a reporter gene. The reporter gene may consist of the native promoter of the gene in question that drives the expression of a readily assayable gene product such as GUS or GFP. The reporter gene may also be a marker of a particular type of physiological response.\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000049"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "reporter gene assay evidence"
        ],
        "synonym": [
            "\"IEP: expression of a reporter gene\" RELATED []"
        ]
    },
    "ECO:0000050": {
        "def": [
            "\"Used when an annotation is made on the basis of for a phenotype description for a species or higher level group that given by an author who explicitly references an observation of a voucher specimen(s). Voucher specimens are defined as those specimens with permanent museum catalog numbers. Thus it would be possible for a person to examine this particular specimen and observe the annotated phenotype.\" [PhenoScape:DOA]"
        ],
        "id": [
            "ECO:0000050"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "voucher specimen analysis evidence"
        ],
        "synonym": [
            "\"IVS\" EXACT []"
        ]
    },
    "ECO:0000051": {
        "comment": [
            "A genetic similarity analysis might consider genetic markers, polymorphisms, alleles, or other characteristics sometimes considered as part of the field of traditional genetics. Although an attempt has been made to treat as distinct the concepts of \"genetic\", \"genotypic\", \"genomic\", and \"sequence\", there is considerable overlap in usage throughout the field of biology."
        ],
        "def": [
            "\"A type of similarity based on genotype without respect to expression.\" [ECO:MCC, PhenoScape:IGTS]"
        ],
        "id": [
            "ECO:0000051"
        ],
        "is_a": [
            "ECO:0000041 ! similarity evidence"
        ],
        "name": [
            "genetic similarity evidence"
        ],
        "synonym": [
            "\"IGTS\" EXACT []",
            "\"inferred from genetic similarity\" RELATED []"
        ]
    },
    "ECO:0000052": {
        "id": [
            "ECO:0000052"
        ],
        "is_a": [
            "ECO:0000011 ! genetic interaction evidence"
        ],
        "name": [
            "suppressor/enhancer interaction evidence"
        ],
        "synonym": [
            "\"IGI: 'traditional' genetic interactions (e.g. suppressors, synthetic lethals)\" RELATED []"
        ],
        "xref": [
            "TAIR:TED\\:0000063"
        ]
    },
    "ECO:0000053": {
        "comment": [
            "Combinatorial analyses could include experimental or computational results. Examples include: (i) large-scale experiment such as a genome-wide two-hybrid or genome-wide synthetic interactions; (ii) integration of large-scale data sets of various types; and (iii) text-based-computation, e.g. text-mining. For simple sequence comparisons, one should use the sequence similarity analysis evidence type. For microarray results alone, expression pattern analysis is appropriate; whereas, large-scale computational analysis should be used when microarray results are combined with the results of other types of large-scale experiments."
        ],
        "def": [
            "\"A type of combinatorial analysis where data are combined and evaluated by an algorithm.\" [ECO:go, ECO:MCC]"
        ],
        "id": [
            "ECO:0000053"
        ],
        "is_a": [
            "ECO:0000212 ! combinatorial evidence"
        ],
        "name": [
            "computational combinatorial evidence"
        ],
        "xref": [
            "TAIR:TED\\:0000004"
        ]
    },
    "ECO:0000054": {
        "def": [
            "\"Used when an annotation is made based on the phenotype of double mutant organism that contains a mutation in the gene product of interest in addition to a second mutation at an unrelated locus.\" [TAIT:TED]"
        ],
        "id": [
            "ECO:0000054"
        ],
        "is_a": [
            "ECO:0000011 ! genetic interaction evidence"
        ],
        "name": [
            "double mutant phenotype evidence"
        ],
        "synonym": [
            "\"IGI: double mutant analysis\" RELATED []"
        ]
    },
    "ECO:0000055": {
        "id": [
            "ECO:0000055"
        ],
        "is_a": [
            "ECO:0000008 ! expression pattern evidence"
        ],
        "name": [
            "array experiment evidence"
        ]
    },
    "ECO:0000056": {
        "def": [
            "\"Used when an annotation is based on results of epistatis analysis of an allele of the gene in question and a mutant allele at another locus.\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000056"
        ],
        "is_a": [
            "ECO:0000011 ! genetic interaction evidence"
        ],
        "name": [
            "epistatic interaction evidence"
        ],
        "synonym": [
            "\"IGI: epistatic interactions\" RELATED []"
        ]
    },
    "ECO:0000057": {
        "comment": [
            "Phenotype is defined as the outcome of the expression of a genotype in a given environment. A comparison might involve whole organisms or sub-parts of organisms."
        ],
        "def": [
            "\"A type of similarity based on the expression of a genotype in an environment.\" [ECO:MCC, PhenoScape:IPTS]"
        ],
        "id": [
            "ECO:0000057"
        ],
        "is_a": [
            "ECO:0000041 ! similarity evidence"
        ],
        "name": [
            "phenotypic similarity evidence"
        ],
        "synonym": [
            "\"inferred from phenotypic similarity\" RELATED []",
            "\"IPTS\" EXACT []"
        ]
    },
    "ECO:0000058": {
        "id": [
            "ECO:0000058"
        ],
        "is_a": [
            "ECO:0000055 ! array experiment evidence"
        ],
        "name": [
            "expression microarray evidence"
        ]
    },
    "ECO:0000059": {
        "alt_id": [
            "ECO:0000014"
        ],
        "def": [
            "\"Experimental evidence that is based on the expression of a genotype in an environment.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000059"
        ],
        "is_a": [
            "ECO:0000006 ! experimental evidence"
        ],
        "name": [
            "experimental phenotypic evidence"
        ],
        "synonym": [
            "\"inferred from phenotype\" RELATED []"
        ]
    },
    "ECO:0000060": {
        "def": [
            "\"Used when an annotation is made based on the similarity of the location and or arrangement of structures.\" [PhenoScape:IPS]"
        ],
        "id": [
            "ECO:0000060"
        ],
        "is_a": [
            "ECO:0000057 ! phenotypic similarity evidence"
        ],
        "name": [
            "positional similarity evidence"
        ],
        "synonym": [
            "\"IPS\" EXACT []"
        ]
    },
    "ECO:0000061": {
        "def": [
            "\"A type of experimental phenotypic evidence where an annotation is made for a gene product that has not been cloned but is associated with a quantitative trait locus.\" [TAIR:tn]"
        ],
        "id": [
            "ECO:0000061"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "quantitative trait analysis evidence"
        ],
        "synonym": [
            "\"IGI: quantitative trait analysis\" RELATED []"
        ]
    },
    "ECO:0000062": {
        "id": [
            "ECO:0000062"
        ],
        "is_a": [
            "ECO:0000055 ! array experiment evidence"
        ],
        "name": [
            "genomic microarray evidence"
        ]
    },
    "ECO:0000063": {
        "def": [
            "\"Used when an annotation is made based on the similarity of the histological makeup of structures.\" [PhenoScape:ICS]"
        ],
        "id": [
            "ECO:0000063"
        ],
        "is_a": [
            "ECO:0000057 ! phenotypic similarity evidence"
        ],
        "name": [
            "compositional similarity evidence"
        ],
        "synonym": [
            "\"ICS\" EXACT []"
        ]
    },
    "ECO:0000064": {
        "def": [
            "\"Used when an annotation is made based on a functional complementation assay in which a wild-type copy of the gene in question is inserted into a heterologous organism with a mutation in the homologous gene.\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000064"
        ],
        "is_a": [
            "ECO:0000012 ! functional complementation evidence"
        ],
        "name": [
            "functional complementation in heterologous system evidence"
        ],
        "synonym": [
            "\"IGI: functional complementation in heterologous system\" RELATED []"
        ]
    },
    "ECO:0000065": {
        "id": [
            "ECO:0000065"
        ],
        "is_a": [
            "ECO:0000055 ! array experiment evidence"
        ],
        "name": [
            "CpG island microarray evidence"
        ]
    },
    "ECO:0000066": {
        "comment": [
            "The assay involves screening a library of candidate proteins for the ability bind to a target, cis-regulatory element or any other short, DNA binding sequence placed 5' to a yeast reporter gene (TAIR:TED)."
        ],
        "def": [
            "\"A type of hybrid interaction evidence that is based on a protein-DNA complementation assay where a single promoter acts as bait and is screened against a library of prey transcription factors.\" [ECO:MCC, PSI-MI:MI\\:0432, TAIR:TED]"
        ],
        "id": [
            "ECO:0000066"
        ],
        "is_a": [
            "ECO:0000025 ! hybrid interaction evidence"
        ],
        "name": [
            "yeast one-hybrid evidence"
        ],
        "synonym": [
            "\"IPI: yeast one-hybrid assay\" RELATED []"
        ],
        "xref": [
            "PSI-MI:MI\\:0432 \"one hybrid\""
        ]
    },
    "ECO:0000067": {
        "def": [
            "\"Used when an annotation is made based on the similarity of embryological and/or post-embryonic origin of structures.\" [PhenoScape:IDS]"
        ],
        "id": [
            "ECO:0000067"
        ],
        "is_a": [
            "ECO:0000057 ! phenotypic similarity evidence"
        ],
        "name": [
            "developmental similarity evidence"
        ],
        "synonym": [
            "\"IDS\" EXACT []"
        ]
    },
    "ECO:0000068": {
        "def": [
            "\"Used when an annotation is based on the interaction between two proteins in a yeast two-hybrid assay. The assay involves screening a trap-tagged library with a bait-tagged vector. The library is tagged with a DNA-binding domain (trap) and the bait is tagged with a transcriptional activation domain. Interaction between a library-trap protein and the bait results in activation of a yeast reporter gene whose transcription can be experimentally assayed.\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000068"
        ],
        "is_a": [
            "ECO:0000025 ! hybrid interaction evidence"
        ],
        "name": [
            "yeast 2-hybrid evidence"
        ],
        "synonym": [
            "\"IPI: yeast two-hybrid assay\" RELATED []"
        ]
    },
    "ECO:0000069": {
        "id": [
            "ECO:0000069"
        ],
        "is_a": [
            "ECO:0000026 ! nucleic acid hybridization evidence"
        ],
        "name": [
            "differential methylation hybridization evidence"
        ]
    },
    "ECO:0000070": {
        "comment": [
            "If performing GO annotation, the interacting protein is referenced in the evidence_with column."
        ],
        "def": [
            "\"A type of immunoprecipitation evidence that involves precipitating two or more proteins via binding to an antibody specific to a single protein, followed by protein identification.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000070"
        ],
        "is_a": [
            "ECO:0000085 ! immunoprecipitation evidence"
        ],
        "name": [
            "co-immunoprecipitation evidence"
        ],
        "synonym": [
            "\"IPI: co-immunoprecipitation\" RELATED []"
        ],
        "xref": [
            "PSI-MI:MI\\:0019 \"coimmunoprecipitation\""
        ]
    },
    "ECO:0000071": {
        "def": [
            "\"Used when an annotation is made based on the similarity of the shape, structure or overall configuration of structures.\" [PhenoScape:IMS]"
        ],
        "id": [
            "ECO:0000071"
        ],
        "is_a": [
            "ECO:0000057 ! phenotypic similarity evidence"
        ],
        "name": [
            "morphological similarity evidence"
        ],
        "synonym": [
            "\"IMS\" EXACT []"
        ]
    },
    "ECO:0000072": {
        "def": [
            "\"A type of physical interaction evidence that is based on a genetic screening system that detects protein-protein interactions and is not based on a transcriptional readout.\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000072"
        ],
        "is_a": [],
        "is_obsolete": [
            "true"
        ],
        "name": [
            "Sos-recruitment assay evidence"
        ],
        "synonym": [
            "\"IPI: Sos-recruitment assay\" RELATED []"
        ]
    },
    "ECO:0000073": {
        "def": [
            "\"Experimental evidence that characterizes an attribute of the genome underlying a gene product.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000073"
        ],
        "is_a": [
            "ECO:0000006 ! experimental evidence"
        ],
        "name": [
            "experimental genomic evidence"
        ],
        "synonym": [
            "\"inferred from genomic analysis\" EXACT []"
        ]
    },
    "ECO:0000074": {
        "def": [
            "\"Used when an annotation is based on the interaction of two proteins in a split-ubiquitin assay. The bait protein is fused to the C-terminal ubiquitin (Cub) domain followed by a reporter protein, and the prey protein is fused to a mutated N terminal ubiquiting (NubG) domain. If bait and prey interact, their interaction brings the NubG and Cub domains close enough together to reconstitute split-ubiquitin, resulting in the release of the reporter protein by the action of the UBPs.\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000074"
        ],
        "is_a": [
            "ECO:0000021 ! physical interaction evidence"
        ],
        "name": [
            "split-ubiquitin assay evidence"
        ],
        "synonym": [
            "\"IPI: split-ubiquitin assay\" RELATED []"
        ],
        "xref": [
            "PSI-MI:MI\\:0112 \"ubiquitin reconstruction\""
        ]
    },
    "ECO:0000075": {
        "def": [
            "\"Used when an annotation is made based on the similarity of expression of genes in structures.\" [PhenoScape:IGES]"
        ],
        "id": [
            "ECO:0000075"
        ],
        "is_a": [
            "ECO:0000057 ! phenotypic similarity evidence"
        ],
        "name": [
            "gene expression similarity evidence"
        ],
        "synonym": [
            "\"IGES\" EXACT []"
        ]
    },
    "ECO:0000076": {
        "def": [
            "\"Used when an annotation is based on the interaction of protein with another in a far-Western assay. The assay involves separating target proteins on an SDS-PAGE gel, blotting to a membrane, hybridization with a protein probe and visualization using a probe-directed antibody. The interacting protein is referenced in the evidence_with column.\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000076"
        ],
        "is_a": [
            "ECO:0000021 ! physical interaction evidence"
        ],
        "name": [
            "far-Western blotting evidence"
        ],
        "synonym": [
            "\"IPI: far-Western analysis\" RELATED []"
        ],
        "xref": [
            "PSI-MI:MI\\:0047"
        ]
    },
    "ECO:0000077": {
        "id": [
            "ECO:0000077"
        ],
        "is_a": [
            "ECO:0000082 ! polymerase chain reaction evidence"
        ],
        "name": [
            "methylation-specific polymerase chain reaction evidence"
        ],
        "synonym": [
            "\"methylation-specific PCR evidence\" EXACT []"
        ]
    },
    "ECO:0000078": {
        "def": [
            "\"Used when an annotation is based on the hybridization of a probe derived from the gene in question with a gene of known function/process/component. Can also be used when a probe derived from a gene of known function/process/component hybridizes with the gene in question.\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000078"
        ],
        "is_a": [
            "ECO:0005519 ! DNA detection assay evidence"
        ],
        "name": [
            "southern hybridization evidence"
        ],
        "synonym": [
            "\"ISS: Southern blotting\" RELATED []",
            "\"Southern blot\" EXACT []"
        ]
    },
    "ECO:0000079": {
        "comment": [
            "\"Used when an annotation is made based on affinity chromatography, which is a selective separation technique by which a compound (e.g., an antibody) is immobilized on a polymeric matrix and used to bind selectively other compounds. Following removal of the unattached components, the bound compound is displaced by changing the concentration of protons, salts, or cofactors in the eluent\" (from original definition by TAIR:TED). Types of highly specific interaction might include those of antigen and antibody, enzyme and substrate, or receptor and ligand."
        ],
        "def": [
            "\"A type of affinity evidence that results from separation of biochemical mixtures by selective binding of a compound to an immobilized compound on a polymeric matrix, subsequent removal of unattached components, and then displacement of the bond compound.\" [ECO:MCC, TAIR:TED]"
        ],
        "id": [
            "ECO:0000079"
        ],
        "is_a": [
            "ECO:0000023 ! affinity evidence"
        ],
        "name": [
            "affinity chromatography evidence"
        ],
        "synonym": [
            "\"IDA: affinity chromatography\" RELATED []"
        ],
        "xref": [
            "PSI-MI:MI\\:0004 \"affinity chromatography technology\""
        ]
    },
    "ECO:0000080": {
        "def": [
            "\"A type of similarity that indicates common ancestry.\" [ECO:MCC, PhenoScape:IP]"
        ],
        "id": [
            "ECO:0000080"
        ],
        "is_a": [
            "ECO:0000041 ! similarity evidence"
        ],
        "name": [
            "phylogenetic evidence"
        ],
        "synonym": [
            "\"IP\" EXACT [PhenoScape:IP]"
        ]
    },
    "ECO:0000081": {
        "def": [
            "\"Used when a component annotation is made based on the presence of a targeting sequence in a protein's primary sequence. The presence of the targeting sequence may be detected by computational prediction and/or the author's manual examination of the sequence.\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000081"
        ],
        "is_a": [
            "ECO:0000028 ! motif similarity evidence"
        ],
        "name": [
            "targeting sequence prediction evidence"
        ],
        "synonym": [
            "\"ISS: targeting sequence prediction\" RELATED []"
        ]
    },
    "ECO:0000082": {
        "id": [
            "ECO:0000082"
        ],
        "is_a": [
            "ECO:0000073 ! experimental genomic evidence"
        ],
        "name": [
            "polymerase chain reaction evidence"
        ],
        "synonym": [
            "\"PCR evidence\" EXACT []"
        ]
    },
    "ECO:0000083": {
        "def": [
            "\"Used when a component annotation is made based on the presence of one or more transmembrane domains in a protein's primary sequence. The presence of the transmembrane domains may be detected by computational prediction and/or the author's manual examination of the sequence.\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000083"
        ],
        "is_a": [
            "ECO:0000028 ! motif similarity evidence"
        ],
        "name": [
            "transmembrane domain prediction evidence"
        ],
        "synonym": [
            "\"ISS: transmembrane domain prediction\" RELATED []"
        ]
    },
    "ECO:0000084": {
        "comment": [
            "Genomic cluster analyses include synteny and operon structure."
        ],
        "created_by": [
            "mgiglio"
        ],
        "creation_date": [
            "2009-03-20T11:55:18Z"
        ],
        "def": [
            "\"Evidence that supports the identity of a gene product on the basis of the identity of neighboring genes.\" [ECO:MCC, GOC:mg]"
        ],
        "id": [
            "ECO:0000084"
        ],
        "is_a": [
            "ECO:0000177 ! genomic context evidence"
        ],
        "name": [
            "gene neighbors evidence"
        ],
        "synonym": [
            "\"ICL\" RELATED []",
            "\"inferred from genome cluster\" EXACT []"
        ],
        "xref": [
            "GO_REF:0000025 \"Operon structure as IGC evidence\""
        ]
    },
    "ECO:0000085": {
        "comment": [
            "Transcription factors isolated in this way can be incubated with a radiolabeled probe to demonstrate binding."
        ],
        "def": [
            "\"A type of protein assay evidence that involves precipitation of a multivalent antigen by a bivalent antibody for protein isolation.\" [ECO:MCC, ECO:SW, TAIR:TED]"
        ],
        "id": [
            "ECO:0000085"
        ],
        "is_a": [
            "ECO:0000039 ! protein assay evidence"
        ],
        "name": [
            "immunoprecipitation evidence"
        ],
        "synonym": [
            "\"IDA: immunoprecipitation\" RELATED []"
        ]
    },
    "ECO:0000086": {
        "id": [
            "ECO:0000086"
        ],
        "is_a": [
            "ECO:0000073 ! experimental genomic evidence"
        ],
        "name": [
            "intermethylated site amplification evidence"
        ]
    },
    "ECO:0000087": {
        "def": [
            "\"A type of microscopy evidence where antibodies are used to detect the location of molecules or other structures within cells or tissues.\" [ECO:MCC, ECO:SN]"
        ],
        "id": [
            "ECO:0000087"
        ],
        "is_a": [
            "ECO:0001098 ! microscopy evidence",
            "ECO:0005593 ! immunodetection assay evidence"
        ],
        "name": [
            "immunolocalization evidence"
        ],
        "synonym": [
            "\"IDA: immunolocalization\" RELATED []"
        ]
    },
    "ECO:0000088": {
        "comment": [
            "The biological system in question might be a multi-step process or pathway or a physical complex comprising several components. The components in the experimental evidence can come from the same species or a mix of species. The experimental evidences may be only partial or weak."
        ],
        "created_by": [
            "mgiglio"
        ],
        "creation_date": [
            "2009-03-20T12:00:17Z"
        ],
        "def": [
            "\"A type of evidence that is based on a combination of experimental evidences or existing models of that system in a related species used for the reconstruction of a biological system.\" [ECO:MCC, GOC:mg]"
        ],
        "id": [
            "ECO:0000088"
        ],
        "is_a": [
            "ECO:0000000 ! evidence"
        ],
        "name": [
            "biological system reconstruction evidence"
        ],
        "synonym": [
            "\"inferred from system reconstruction\" RELATED []",
            "\"ISR\" RELATED []"
        ]
    },
    "ECO:0000089": {
        "alt_id": [
            "ECO:0001125"
        ],
        "id": [
            "ECO:0000089"
        ],
        "is_a": [
            "ECO:0005519 ! DNA detection assay evidence"
        ],
        "name": [
            "restriction landmark genomic scanning evidence"
        ],
        "synonym": [
            "\"restriction landmark genome scanning evidence\" EXACT []",
            "\"RLGS evidence\" EXACT []"
        ]
    },
    "ECO:0000090": {
        "def": [
            "\"A type of immunolocalization evidence where the antibodies are labeled with colloidal gold particles whose location is then detected via microscopy.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000090"
        ],
        "is_a": [
            "ECO:0000087 ! immunolocalization evidence"
        ],
        "name": [
            "immunogold labelling evidence"
        ],
        "synonym": [
            "\"IDA: immunogold labelling\" RELATED []"
        ]
    },
    "ECO:0000092": {
        "def": [
            "\"Used when an annotation is made based on immunolocalization of recombinant proteins fused with epitopes that are recognized by antibodies.\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000092"
        ],
        "is_a": [
            "ECO:0000087 ! immunolocalization evidence"
        ],
        "name": [
            "epitope-tagged protein immunolocalization evidence"
        ],
        "synonym": [
            "\"IDA: immunolocalization of epitope-tagged protein\" RELATED []"
        ]
    },
    "ECO:0000093": {
        "id": [
            "ECO:0000093"
        ],
        "is_a": [
            "ECO:0000055 ! array experiment evidence"
        ],
        "name": [
            "oligonucleotide microarray evidence"
        ]
    },
    "ECO:0000094": {
        "def": [
            "\"Direct assay evidence that is derived by using a living organism to measure the effect of a substance, factor, or condition.\" [ECO:MCC, TAIR:TED]"
        ],
        "id": [
            "ECO:0000094"
        ],
        "is_a": [
            "ECO:0000002 ! direct assay evidence"
        ],
        "name": [
            "biological assay evidence"
        ],
        "synonym": [
            "\"IDA: bioassay\" RELATED []"
        ]
    },
    "ECO:0000095": {
        "comment": [
            "Cell growth aspects can include growth rate and extent of growth. A cell growth curve acts as a natural reporter."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-10-15T00:58:50Z"
        ],
        "def": [
            "\"A type of in vivo assay evidence that measures one or more aspects of cell growth over a specified time period to indicate an induced or repressed regulatory effect.\" [ECO:SW, PomBase:MAH]"
        ],
        "id": [
            "ECO:0000095"
        ],
        "is_a": [
            "ECO:0000178 ! in vivo assay evidence"
        ],
        "name": [
            "cell growth assay evidence"
        ],
        "synonym": [
            "\"growth curve analysis\" RELATED []"
        ]
    },
    "ECO:0000096": {
        "comment": [
            "EMSA is often used for assessing TF-binding with fluorophore labelling. Protein-nucleic acid complexes generally migrate at a slower rate than the corresponding non-bonded nucleic acid."
        ],
        "def": [
            "\"A type of direct assay evidence based on an electrophoretic mobility shift of macromolecules, compounds, and proteins where solutions of protein and nucleic acid are combined and the resulting mixtures are electrophoresed under native conditions through polyacrylamide or agarose gel to detect protein-nucleic acid interactions.\" [ECO:KIM, ECO:SW, PMID:17703195, TAIR:TED]"
        ],
        "id": [
            "ECO:0000096"
        ],
        "is_a": [
            "ECO:0000002 ! direct assay evidence"
        ],
        "name": [
            "electrophoretic mobility shift assay evidence"
        ],
        "synonym": [
            "\"EMSA: electrophoretic mobility shift assay\" EXACT []",
            "\"Gel retardation assay\" RELATED [PMID:17703195]",
            "\"IDA: electrophoretic mobility shift assay\" RELATED []"
        ],
        "xref": [
            "PSI-MI:MI\\:0413 \"electrophoretic mobility shift assay\""
        ]
    },
    "ECO:0000097": {
        "id": [
            "ECO:0000097"
        ],
        "is_a": [
            "ECO:0000055 ! array experiment evidence"
        ],
        "name": [
            "cDNA microarray evidence"
        ]
    },
    "ECO:0000098": {
        "def": [
            "\"Used when an annotation is made based on a method that hybridizes a labeled nucleic acid probe to a tissue or cell specimen in which the cellular structures are still intact. Depending on the label and protocol used, the probe may detect complementary DNA or RNA by hybridizing specifically to that gene or sequence.\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000098"
        ],
        "is_a": [
            "ECO:0000026 ! nucleic acid hybridization evidence"
        ],
        "name": [
            "in situ hybridization evidence"
        ],
        "synonym": [
            "\"IDA: in situ hybridization\" RELATED []"
        ]
    },
    "ECO:0000100": {
        "id": [
            "ECO:0000100"
        ],
        "is_a": [
            "ECO:0000002 ! direct assay evidence"
        ],
        "name": [
            "fractionation evidence"
        ]
    },
    "ECO:0000101": {
        "id": [
            "ECO:0000101"
        ],
        "is_a": [
            "ECO:0000055 ! array experiment evidence"
        ],
        "name": [
            "Affymetrix array experiment evidence"
        ]
    },
    "ECO:0000102": {
        "def": [
            "\"Used when an annotation is made based on fractionation of a protein with other compounds, factors, or macromolecules.\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000102"
        ],
        "is_a": [
            "ECO:0000100 ! fractionation evidence"
        ],
        "name": [
            "co-fractionation evidence"
        ],
        "synonym": [
            "\"IDA: co-fractionation\" RELATED []"
        ]
    },
    "ECO:0000104": {
        "def": [
            "\"Used when an annotation is made based on levels of RNA expression determined by microarray experiment.\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000104"
        ],
        "is_a": [
            "ECO:0000048 ! transcript expression level evidence",
            "ECO:0000058 ! expression microarray evidence"
        ],
        "name": [
            "microarray RNA expression level evidence"
        ],
        "synonym": [
            "\"IEP: transcript levels (e.g. microarray data)\" RELATED []"
        ]
    },
    "ECO:0000105": {
        "id": [
            "ECO:0000105"
        ],
        "is_a": [
            "ECO:0000055 ! array experiment evidence"
        ],
        "name": [
            "Nimblegen array evidence"
        ]
    },
    "ECO:0000106": {
        "def": [
            "\"A type of transcript expression level evidence based on electrophoresis and probing to determine levels of RNA expression using a complementary hybridization probe on the separated RNA samples.\" [ECO:SW, TAIR:TED]"
        ],
        "id": [
            "ECO:0000106"
        ],
        "is_a": [
            "ECO:0000048 ! transcript expression level evidence"
        ],
        "name": [
            "northern assay evidence"
        ],
        "synonym": [
            "\"IEP: transcript levels (e.g. Northerns)\" RELATED []",
            "\"Northern blot\" EXACT []"
        ]
    },
    "ECO:0000108": {
        "def": [
            "\"Used when an annotation is made based on levels of RNA expression determined by an RT-PCR experiment (quantitative or otherwise).\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000108"
        ],
        "is_a": [
            "ECO:0000048 ! transcript expression level evidence",
            "ECO:0000109 ! reverse transcription polymerase chain reaction evidence"
        ],
        "name": [
            "reverse transcription polymerase chain reaction transcription evidence"
        ],
        "synonym": [
            "\"IEP: transcript levels (e.g. RT-PCR)\" RELATED []",
            "\"RT-PCR transcription evidence\" EXACT []"
        ]
    },
    "ECO:0000109": {
        "comment": [
            "The starting product for PCR, and therefore amplification volume, is directly correlated to the transcription rate."
        ],
        "def": [
            "\"A type of polymerase chain reaction evidence where RNA is reverse transcribed into cDNA and amplified for product measurement in real time for transcription analysis i.e. qPCR.\" [ECO:SW, PMID:11013345, PMID:12901609]"
        ],
        "id": [
            "ECO:0000109"
        ],
        "is_a": [
            "ECO:0000082 ! polymerase chain reaction evidence"
        ],
        "name": [
            "reverse transcription polymerase chain reaction evidence"
        ],
        "synonym": [
            "\"qRT-PCR\" EXACT [PMID:12901609]",
            "\"RT-PCR evidence\" EXACT []"
        ]
    },
    "ECO:0000110": {
        "def": [
            "\"A type of nuclease protection assay evidence where mRNA is hybridized with radiolabeled RNA probes after which RNAse is added to digest the unbound, nonresistant single-stranded overhang regions, later the remaining probe target hybrids are purified and resolved on denaturing polyacrylamide gel, and quantified by autoradiography.\" [ECO:SW, PMID:23457339, TAIR:TED]"
        ],
        "id": [
            "ECO:0000110"
        ],
        "is_a": [
            "ECO:0001106 ! nuclease protection assay evidence"
        ],
        "name": [
            "RNA protection assay evidence"
        ],
        "synonym": [
            "\"IEP: RNA protection assay\" RELATED []",
            "\"ribonuclease protection assay\" EXACT []",
            "\"RNAse protection assay\" RELATED []",
            "\"RPA\" NARROW []"
        ]
    },
    "ECO:0000112": {
        "comment": [
            "Western blot is used for protein detection and analysis. A mixed protein sample is separated through polyacrylamide gel electrophoresis then transferred to a membrane, such as polyvinylidene fluoride (PVDF), and labeled with protein-specific antibodies."
        ],
        "def": [
            "\"A type of gel electrophoresis evidence where proteins are separated using gel electrophoresis and then they are blotted on a nitrocellulose or polyvinylidene difluoride (PVDF) membrane, later an antibody with a dye or an enzyme is added to the solution which is able to bind to its specific protein and this product is detected either by chemiluminescent detection technique or fluorescence imaging technique.\" [ECO:SW, PMID:23050259, TAIR:TED]"
        ],
        "id": [
            "ECO:0000112"
        ],
        "is_a": [
            "ECO:0000333 ! sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence",
            "ECO:0005593 ! immunodetection assay evidence"
        ],
        "name": [
            "western blot evidence"
        ],
        "synonym": [
            "\"IEP: protein levels (e.g. Western blots)\" RELATED []",
            "\"protein immunoblot\" RELATED []",
            "\"Western blot expression analysis\" RELATED []"
        ]
    },
    "ECO:0000114": {
        "def": [
            "\"A type of protein expression evidence where a protein of interest is isolated on the basis of its hybridization to an antibody raised to a homologous protein.\" [ECO:MCC, TAIR:TED]"
        ],
        "id": [
            "ECO:0000114"
        ],
        "is_a": [
            "ECO:0000010 ! protein expression evidence"
        ],
        "name": [
            "expression library screen evidence"
        ],
        "synonym": [
            "\"IEP: expression library screening\" RELATED []"
        ]
    },
    "ECO:0000116": {
        "def": [
            "\"Used when an annotation is made based on results of a differential hybridization experiment that demonstrates expression of an RNA under one condition and not another.\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000116"
        ],
        "is_a": [
            "ECO:0000009 ! transcript expression evidence",
            "ECO:0000026 ! nucleic acid hybridization evidence"
        ],
        "name": [
            "differential hybridization evidence"
        ],
        "synonym": [
            "\"IEP: differential hybridization\" RELATED []"
        ]
    },
    "ECO:0000118": {
        "def": [
            "\"Used when an annotation is made based on results of a subtractive hybridization experiment that demonstrates expression of an RNA under one condition and not another.\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000118"
        ],
        "is_a": [
            "ECO:0000009 ! transcript expression evidence",
            "ECO:0000026 ! nucleic acid hybridization evidence"
        ],
        "name": [
            "subtractive hybridization evidence"
        ],
        "synonym": [
            "\"IEP: subtractive hybridization\" RELATED []"
        ]
    },
    "ECO:0000120": {
        "def": [
            "\"Used when an annotation is made based on the analysis of the phenotype of a wild-type or mutant transgenic organism that has been engineered to overexpress the gene product in question.\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000120"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "over expression analysis evidence"
        ],
        "synonym": [
            "\"IMP: analysis of overexpression/ectopic expression phenotype\" RELATED []"
        ]
    },
    "ECO:0000122": {
        "id": [
            "ECO:0000122"
        ],
        "is_a": [
            "ECO:0000039 ! protein assay evidence"
        ],
        "name": [
            "protein localization evidence"
        ]
    },
    "ECO:0000124": {
        "id": [
            "ECO:0000124"
        ],
        "is_a": [
            "ECO:0000122 ! protein localization evidence"
        ],
        "name": [
            "fusion protein localization evidence"
        ]
    },
    "ECO:0000126": {
        "id": [
            "ECO:0000126"
        ],
        "is_a": [
            "ECO:0000124 ! fusion protein localization evidence"
        ],
        "name": [
            "green fluorescent protein fusion protein localization evidence"
        ],
        "synonym": [
            "\"GFP fusion protein localization evidence\" EXACT []",
            "\"IDA: localization of GFP/YFP fusion protein\" RELATED []"
        ]
    },
    "ECO:0000128": {
        "id": [
            "ECO:0000128"
        ],
        "is_a": [
            "ECO:0000124 ! fusion protein localization evidence"
        ],
        "name": [
            "yellow fluorescent protein fusion protein localization evidence"
        ],
        "synonym": [
            "\"IDA: localization of GFP/YFP fusion protein\" RELATED []",
            "\"YFP fusion protein localization evidence\" EXACT []"
        ]
    },
    "ECO:0000130": {
        "id": [
            "ECO:0000130"
        ],
        "is_a": [
            "ECO:0000124 ! fusion protein localization evidence"
        ],
        "name": [
            "beta-glucuronidase fusion protein localization evidence"
        ],
        "synonym": [
            "\"GUS fusion protein localization evidence\" EXACT []",
            "\"GUS staining evidence\" NARROW []",
            "\"IDA: localization of GUS fusion protein\" RELATED []"
        ]
    },
    "ECO:0000132": {
        "id": [
            "ECO:0000132"
        ],
        "is_a": [
            "ECO:0000124 ! fusion protein localization evidence"
        ],
        "name": [
            "beta-galactosidase fusion protein localization evidence"
        ],
        "synonym": [
            "\"LacZ fusion protein localization evidence\" EXACT []"
        ]
    },
    "ECO:0000134": {
        "id": [
            "ECO:0000134"
        ],
        "is_a": [
            "ECO:0000002 ! direct assay evidence"
        ],
        "name": [
            "transport assay evidence"
        ],
        "synonym": [
            "\"IDA: transport assay\" RELATED []"
        ]
    },
    "ECO:0000136": {
        "id": [
            "ECO:0000136"
        ],
        "is_a": [
            "ECO:0000023 ! affinity evidence"
        ],
        "name": [
            "nucleic acid binding evidence"
        ]
    },
    "ECO:0000138": {
        "id": [
            "ECO:0000138"
        ],
        "is_a": [
            "ECO:0000136 ! nucleic acid binding evidence"
        ],
        "name": [
            "ribohomopolymer binding assay evidence"
        ],
        "synonym": [
            "\"IDA: ribohomopolymer binding assay\" RELATED []"
        ]
    },
    "ECO:0000140": {
        "def": [
            "\"A type of chromatography evidence that uses a thin layer of adsorbent (such as silica gel, alumina, or cellulose) on a flat, inert substrate.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000140"
        ],
        "is_a": [
            "ECO:0000325 ! chromatography evidence"
        ],
        "name": [
            "thin layer chromatography evidence"
        ],
        "synonym": [
            "\"IDA: thin layer chromatography\" RELATED []",
            "\"TLC evidence\" EXACT []"
        ]
    },
    "ECO:0000142": {
        "id": [
            "ECO:0000142"
        ],
        "is_a": [
            "ECO:0000024 ! protein binding evidence"
        ],
        "name": [
            "protein:ion binding evidence"
        ]
    },
    "ECO:0000144": {
        "def": [
            "\"Used when an annotation is made based on a Southwestern blot analysis, which detects DNA binding of proteins using labeled DNA as probe hybridizing to electrophoretically separated proteins.\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000144"
        ],
        "is_a": [
            "ECO:0000136 ! nucleic acid binding evidence"
        ],
        "name": [
            "Southwestern blot evidence"
        ],
        "synonym": [
            "\"IDA: Southwestern analysis\" RELATED []"
        ]
    },
    "ECO:0000146": {
        "def": [
            "\"Used when an annotation is made based on a Northwestern blot analysis, which detects RNA binding of proteins using labeled RNA as probe hybridizing to electrophoretically separated proteins.\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000146"
        ],
        "is_a": [
            "ECO:0000136 ! nucleic acid binding evidence"
        ],
        "name": [
            "Northwestern blot evidence"
        ],
        "synonym": [
            "\"IDA: Northwestern analysis\" RELATED []"
        ]
    },
    "ECO:0000148": {
        "id": [
            "ECO:0000148"
        ],
        "is_a": [],
        "is_obsolete": [
            "true"
        ],
        "name": [
            "in vitro binding evidence"
        ],
        "synonym": [
            "\"IDA: in vitro binding assay\" RELATED []"
        ]
    },
    "ECO:0000150": {
        "id": [
            "ECO:0000150"
        ],
        "is_a": [
            "ECO:0000003 ! reconstitution assay evidence"
        ],
        "name": [
            "in vitro transcription reconstitution assay evidence"
        ],
        "synonym": [
            "\"IDA: in vitro reconstitution assay with recombinant protein\" RELATED []"
        ]
    },
    "ECO:0000152": {
        "id": [
            "ECO:0000152"
        ],
        "is_a": [
            "ECO:0000150 ! in vitro transcription reconstitution assay evidence"
        ],
        "name": [
            "in vitro recombinant protein transcription reconstitution assay evidence"
        ],
        "synonym": [
            "\"IDA: in vitro reconstitution assay with recombinant protein\" RELATED []"
        ]
    },
    "ECO:0000154": {
        "def": [
            "\"A type of protein expression evidence where a gene from one cell is inserted into a cell that does not typically contain that gene and heterologous protein expression is assessed.\" [ECO:KAV]"
        ],
        "id": [
            "ECO:0000154"
        ],
        "is_a": [
            "ECO:0000010 ! protein expression evidence"
        ],
        "name": [
            "heterologous protein expression evidence"
        ],
        "synonym": [
            "\"IDA: protein expression in heterologous system\" RELATED []"
        ]
    },
    "ECO:0000156": {
        "id": [
            "ECO:0000156"
        ],
        "is_a": [
            "ECO:0000100 ! fractionation evidence"
        ],
        "name": [
            "protein separation evidence"
        ]
    },
    "ECO:0000158": {
        "id": [
            "ECO:0000158"
        ],
        "is_a": [
            "ECO:0000156 ! protein separation evidence"
        ],
        "name": [
            "protein separation followed by direct sequencing evidence"
        ],
        "synonym": [
            "\"IDA: protein separation and direct sequencing\" RELATED []"
        ]
    },
    "ECO:0000160": {
        "id": [
            "ECO:0000160"
        ],
        "is_a": [
            "ECO:0000156 ! protein separation evidence"
        ],
        "name": [
            "protein separation followed by fragment identification evidence"
        ],
        "synonym": [
            "\"IDA: protein separation and fragment identification\" RELATED []"
        ]
    },
    "ECO:0000162": {
        "def": [
            "\"Used when an annotation is made based on small molecule update assays carried out in a heterologous system that contains a recombinant protein.\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000162"
        ],
        "is_a": [
            "ECO:0000134 ! transport assay evidence"
        ],
        "name": [
            "heterologous system uptake evidence"
        ],
        "synonym": [
            "\"IDA: uptake assay in heterologous system\" RELATED []"
        ]
    },
    "ECO:0000164": {
        "comment": [
            "The scale of electrophysiology assays can range from small, e.g. a single ion channel, to large, e.g. an entire organ such as a heart."
        ],
        "def": [
            "\"A type of direct assay evidence where electrical properties of cells or tissues are studied.\" [ECO:KAV]"
        ],
        "id": [
            "ECO:0000164"
        ],
        "is_a": [
            "ECO:0000002 ! direct assay evidence"
        ],
        "name": [
            "electrophysiology assay evidence"
        ]
    },
    "ECO:0000166": {
        "def": [
            "\"A type of electrophysiology assay evidence which involves using two electrodes inserted into a large cell (often a xenopus oocyte) where one electrode is used to measure the internal potential (voltage) of the cell and the other electrode is used to clamp the current.\" [ECO:SN, PMID:23529422]"
        ],
        "id": [
            "ECO:0000166"
        ],
        "is_a": [
            "ECO:0000164 ! electrophysiology assay evidence"
        ],
        "name": [
            "two-electrode voltage clamp experiment evidence"
        ],
        "synonym": [
            "\"IDA: two-electrode voltage clamp technique\" RELATED []"
        ]
    },
    "ECO:0000168": {
        "id": [
            "ECO:0000168"
        ],
        "is_a": [
            "ECO:0000002 ! direct assay evidence"
        ],
        "name": [
            "transcription assay"
        ]
    },
    "ECO:0000170": {
        "def": [
            "\"Used when an annotation is made based on assays that show transcriptional activation of a specific target reporter gene.\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000170"
        ],
        "is_a": [
            "ECO:0000168 ! transcription assay"
        ],
        "name": [
            "transcriptional activation assay"
        ],
        "synonym": [
            "\"IDA: transcriptional activation assay\" RELATED []"
        ]
    },
    "ECO:0000172": {
        "def": [
            "\"Used when an annotation is made based on the analysis of a biochemical aspect of a mutant phenotype; for example, the accumulation of a biosynthetic intermediate.\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000172"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "biochemical trait analysis evidence"
        ],
        "synonym": [
            "\"IMP: analysis of biochemical trait\" RELATED []"
        ]
    },
    "ECO:0000174": {
        "def": [
            "\"Used when an annotation is made based on the physiological response of a mutant to an external stimulus; for example, abnormal growth of the root in response to gravity, delay in flowering in response to varying light conditions.\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000174"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "mutant physiological response evidence"
        ],
        "synonym": [
            "\"IMP: analysis of physiological response\" RELATED []"
        ]
    },
    "ECO:0000176": {
        "def": [
            "\"Used when an annotation is made based on the visual examination of a mutant phenotype; for example, abnormalities in organism morphology.\" [TAIR:TED]"
        ],
        "id": [
            "ECO:0000176"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "mutant visible phenotype evidence"
        ],
        "synonym": [
            "\"IMP: analysis of visible trait\" RELATED []"
        ]
    },
    "ECO:0000177": {
        "comment": [
            "This type of evidence might include identity of neighboring genes, operon structure, synteny, phylogenetic analysis, or other whole-genome analysis."
        ],
        "def": [
            "\"An evidence type that is based on the location of a gene, which gives rise to a gene product, within the genome.\" [ECO:go, ECO:MCC]"
        ],
        "id": [
            "ECO:0000177"
        ],
        "is_a": [
            "ECO:0000000 ! evidence"
        ],
        "name": [
            "genomic context evidence"
        ]
    },
    "ECO:0000178": {
        "id": [
            "ECO:0000178"
        ],
        "is_a": [
            "ECO:0000094 ! biological assay evidence"
        ],
        "name": [
            "in vivo assay evidence"
        ]
    },
    "ECO:0000179": {
        "def": [
            "\"A type of experimental evidence arising from the investigation of an animal model system.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000179"
        ],
        "is_a": [
            "ECO:0000006 ! experimental evidence"
        ],
        "name": [
            "animal model system study evidence"
        ]
    },
    "ECO:0000180": {
        "def": [
            "\"A type of experimental evidence arising from a controlled investigation that uses human subjects.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000180"
        ],
        "is_a": [
            "ECO:0000006 ! experimental evidence"
        ],
        "name": [
            "clinical study evidence"
        ]
    },
    "ECO:0000181": {
        "id": [
            "ECO:0000181"
        ],
        "is_a": [
            "ECO:0000094 ! biological assay evidence"
        ],
        "name": [
            "in vitro assay evidence"
        ]
    },
    "ECO:0000182": {
        "id": [
            "ECO:0000182"
        ],
        "is_a": [
            "ECO:0000094 ! biological assay evidence"
        ],
        "name": [
            "in vitro culture assay evidence"
        ]
    },
    "ECO:0000183": {
        "id": [
            "ECO:0000183"
        ],
        "is_a": [
            "ECO:0000181 ! in vitro assay evidence"
        ],
        "name": [
            "cell-free assay evidence"
        ],
        "synonym": [
            "\"in vitro assay\" BROAD []"
        ]
    },
    "ECO:0000184": {
        "def": [
            "\"A type of specific protein inhibition evidence where the protein under study is an enzyme.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000184"
        ],
        "is_a": [
            "ECO:0000005 ! enzyme assay evidence",
            "ECO:0000020 ! specific protein inhibition evidence"
        ],
        "name": [
            "enzyme inhibition evidence"
        ]
    },
    "ECO:0000200": {
        "alt_id": [
            "ECO:00000057"
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-03-18T12:21:31Z"
        ],
        "def": [
            "\"A type of sequence similarity evidence where a sequence alignment is the basis for making an annotation, but only when the alignment has been manually reviewed; or if the information is in a published paper, the authors have manually reviewed the evidence. Such alignments may be pairwise alignments or multiple alignments.\" [url:http\\://www.geneontology.org/GO.evidence.shtml]"
        ],
        "id": [
            "ECO:0000200"
        ],
        "is_a": [
            "ECO:0000044 ! sequence similarity evidence"
        ],
        "name": [
            "sequence alignment evidence"
        ],
        "synonym": [
            "\"inferred from sequence alignment\" EXACT [GO:ISA]",
            "\"ISA\" EXACT [GO:ISA]"
        ]
    },
    "ECO:0000201": {
        "alt_id": [
            "ECO:00000060"
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-03-18T12:30:06Z"
        ],
        "def": [
            "\"Orthology is a relationship between genes in different species indicating that the genes derive from a common ancestor. Orthology is established by multiple criteria generally including amino acid and/or nucleotide sequence comparisons and one or more of the following: phylogenetic analysis, coincident expression, conserved map location, functional complementation, immunological cross-reaction, similarity in subcellular localization, subunit structure, substrate specificity, and response to specific inhibitors.\" [url:http\\://www.geneontology.org/GO.evidence.shtml]"
        ],
        "id": [
            "ECO:0000201"
        ],
        "is_a": [
            "ECO:0000044 ! sequence similarity evidence"
        ],
        "name": [
            "sequence orthology evidence"
        ],
        "synonym": [
            "\"inferred from sequence orthology\" EXACT [GO:ISO]",
            "\"ISO\" EXACT [GO:ISO]",
            "\"ortholog evidence\" RELATED []"
        ]
    },
    "ECO:0000202": {
        "alt_id": [
            "ECO:00000063"
        ],
        "comment": [
            "Used when evidence from any kind of statistical model of a sequence or group of sequences is used to make a prediction about the function of a protein or RNA. Examples of relevant evidence are: Hidden Markov Models, PROSITE motifs, and tools such as tRNASCAN."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-03-18T12:32:30Z"
        ],
        "def": [
            "\"Sequence similarity evidence that is based on a sequence-based statistical model that predicts the function of a gene product.\" [ECO:MCC, url:http\\://www.geneontology.org/GO.evidence.shtml]"
        ],
        "id": [
            "ECO:0000202"
        ],
        "is_a": [
            "ECO:0000044 ! sequence similarity evidence"
        ],
        "name": [
            "match to sequence model evidence"
        ],
        "synonym": [
            "\"inferred from sequence model\" EXACT [GO:ISM]",
            "\"ISM\" EXACT [GO:ISM]"
        ]
    },
    "ECO:0000203": {
        "alt_id": [
            "ECO:00000067"
        ],
        "comment": [
            "An automatic assertion is based on computationally generated information that is not reviewed by a person prior to making the assertion. For example, one common type of automatic assertion involves creating an association of evidence with an entity based on commonness of attributes of that entity and another entity for which an assertion already exists. The commonness is determined algorithmically."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-03-18T12:36:04Z"
        ],
        "def": [
            "\"An assertion method that does not involve human review.\" [ECO:MCC]"
        ],
        "disjoint_from": [
            "ECO:0000218 ! manual assertion"
        ],
        "id": [
            "ECO:0000203"
        ],
        "is_a": [
            "ECO:0000217 ! assertion method"
        ],
        "name": [
            "automatic assertion"
        ]
    },
    "ECO:0000204": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-06-21T11:17:21Z"
        ],
        "def": [
            "\"An evidence type that is based on an assertion by the author of a paper, which is read by a curator.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000204"
        ],
        "is_a": [
            "ECO:0000000 ! evidence"
        ],
        "name": [
            "author statement"
        ],
        "union_of": [
            "ECO:0000033 ! traceable author statement",
            "ECO:0000034 ! non-traceable author statement"
        ]
    },
    "ECO:0000205": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-08-18T05:55:12Z"
        ],
        "def": [
            "\"A type of inferential evidence that is based on a conclusion drawn by a curator.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000205"
        ],
        "is_a": [
            "ECO:0000361 ! inferential evidence"
        ],
        "name": [
            "curator inference"
        ]
    },
    "ECO:0000206": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-08-06T04:48:24Z"
        ],
        "def": [
            "\"A type of pairwise sequence alignment evidence obtained with basic local alignment search tool (BLAST).\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000206"
        ],
        "is_a": [
            "ECO:0005554 ! pairwise sequence alignment evidence"
        ],
        "name": [
            "BLAST evidence"
        ]
    },
    "ECO:0000207": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-08-06T04:49:58Z"
        ],
        "def": [
            "\"BLAST evidence from aligning nucleotide sequence with nucleotide sequence.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000207"
        ],
        "is_a": [
            "ECO:0000206 ! BLAST evidence"
        ],
        "name": [
            "nucleotide BLAST evidence"
        ]
    },
    "ECO:0000208": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-08-06T04:50:45Z"
        ],
        "def": [
            "\"BLAST evidence from aligning amino acid or translated nucleotide sequence with amino acid or translated nucleotide sequence.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000208"
        ],
        "is_a": [
            "ECO:0000206 ! BLAST evidence"
        ],
        "name": [
            "protein BLAST evidence"
        ]
    },
    "ECO:0000209": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-08-06T04:55:21Z"
        ],
        "def": [
            "\"A type of BLAST evidence that is used in an automatic assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000209"
        ],
        "intersection_of": [
            "ECO:0000206 ! BLAST evidence",
            "used_in ECO:0000203 ! automatic assertion"
        ],
        "is_a": [
            "ECO:0000206 ! BLAST evidence",
            "ECO:0005561 {is_inferred=\"true\"} ! pairwise sequence alignment evidence used in automatic assertion"
        ],
        "name": [
            "BLAST evidence used in automatic assertion"
        ],
        "relationship": [
            "used_in ECO:0000203 {is_inferred=\"true\"} ! automatic assertion"
        ]
    },
    "ECO:0000210": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-08-06T05:09:51Z"
        ],
        "def": [
            "\"Nucleotide BLAST evidence that is used in an automatic assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000210"
        ],
        "intersection_of": [
            "ECO:0000207 ! nucleotide BLAST evidence",
            "used_in ECO:0000203 ! automatic assertion"
        ],
        "is_a": [
            "ECO:0000207 ! nucleotide BLAST evidence",
            "ECO:0000209 {is_inferred=\"true\"} ! BLAST evidence used in automatic assertion"
        ],
        "name": [
            "nucleotide BLAST evidence used in automatic assertion"
        ],
        "relationship": [
            "used_in ECO:0000203 {is_inferred=\"true\"} ! automatic assertion"
        ]
    },
    "ECO:0000211": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-08-06T05:11:04Z"
        ],
        "def": [
            "\"Protein BLAST evidence that is used in an automatic assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000211"
        ],
        "intersection_of": [
            "ECO:0000208 ! protein BLAST evidence",
            "used_in ECO:0000203 ! automatic assertion"
        ],
        "is_a": [
            "ECO:0000208 ! protein BLAST evidence",
            "ECO:0000209 {is_inferred=\"true\"} ! BLAST evidence used in automatic assertion"
        ],
        "name": [
            "protein BLAST evidence used in automatic assertion"
        ],
        "relationship": [
            "used_in ECO:0000203 {is_inferred=\"true\"} ! automatic assertion"
        ]
    },
    "ECO:0000212": {
        "alt_id": [
            "ECO:0000036",
            "ECO:0000043"
        ],
        "comment": [
            "A key aspect of this type of evidence is that two or more pieces of information are combined to generate an emergent type of evidence not possible with the constituent pieces of evidence alone. A combinatorial analysis typically involves incorporation of different types of evidence."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-08-06T05:24:57Z"
        ],
        "def": [
            "\"A type of evidence that arises out of the integration of more than one line of evidence.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000212"
        ],
        "is_a": [
            "ECO:0000000 ! evidence"
        ],
        "name": [
            "combinatorial evidence"
        ],
        "synonym": [
            "\"inferred from in-silico analysis\" RELATED []"
        ]
    },
    "ECO:0000213": {
        "comment": [
            "Combinatorial analyses could include experimental or computational results. Examples include: (i) large-scale experiment such as a genome-wide two-hybrid or genome-wide synthetic interactions; (ii) integration of large-scale data sets of various types; and (iii) text-based-computation, e.g. text-mining. For simple sequence comparisons, one should use the sequence similarity analysis evidence type. For microarray results alone, expression pattern analysis is appropriate; whereas, large-scale computational analysis should be used when microarray results are combined with the results of other types of large-scale experiments."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-08-06T05:28:33Z"
        ],
        "def": [
            "\"A type of combinatorial analysis that is used in an automatic assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000213"
        ],
        "intersection_of": [
            "ECO:0000212 ! combinatorial evidence",
            "used_in ECO:0000203 ! automatic assertion"
        ],
        "is_a": [
            "ECO:0000212 ! combinatorial evidence",
            "ECO:0000501 {is_inferred=\"true\"} ! evidence used in automatic assertion"
        ],
        "name": [
            "combinatorial evidence used in automatic assertion"
        ],
        "relationship": [
            "used_in ECO:0000203 {is_inferred=\"true\"} ! automatic assertion"
        ]
    },
    "ECO:0000214": {
        "comment": [
            "This type of evidence can be used in support of both positive and \"not\" GO annotations. It should be used to annotate ancestral genes but not extant ones."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-10-05T11:16:50Z"
        ],
        "def": [
            "\"A type of phylogenetic evidence whereby an aspect of an ancestral gene is inferred through the characterization of an aspect of a descendant gene.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000214"
        ],
        "is_a": [
            "ECO:0000080 ! phylogenetic evidence"
        ],
        "name": [
            "biological aspect of descendant evidence"
        ]
    },
    "ECO:0000215": {
        "comment": [
            "Long branch lengths indicate that descendant sequences may have acquired different or additional functions than the ancestral sequence."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-10-05T03:41:34Z"
        ],
        "def": [
            "\"A type of phylogenetic evidence characterized by long phylogenetic tree branch lengths following a duplication event.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000215"
        ],
        "is_a": [
            "ECO:0000080 ! phylogenetic evidence"
        ],
        "name": [
            "rapid divergence from ancestral sequence evidence"
        ]
    },
    "ECO:0000216": {
        "comment": [
            "The loss of certain residues important for function can indicate that a sequence lacks a particular function."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-10-05T03:43:17Z"
        ],
        "def": [
            "\"A type of phylogenetic evidence characterized by the absence of key sequence residues.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000216"
        ],
        "is_a": [
            "ECO:0000080 ! phylogenetic evidence"
        ],
        "name": [
            "phylogenetic determination of loss of key residues evidence"
        ]
    },
    "ECO:0000217": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-11-12T04:17:37Z"
        ],
        "def": [
            "\"A means by which a statement is made about an entity.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000217"
        ],
        "is_a": [],
        "name": [
            "assertion method"
        ],
        "union_of": [
            "ECO:0000203 ! automatic assertion",
            "ECO:0000218 ! manual assertion"
        ]
    },
    "ECO:0000218": {
        "comment": [
            "A manual assertion could be based on evidence that is generated by and interpreted by a human or it could involve human review of computationally generated information."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-11-12T04:20:02Z"
        ],
        "def": [
            "\"An assertion method that involves human review.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000218"
        ],
        "is_a": [
            "ECO:0000217 ! assertion method"
        ],
        "name": [
            "manual assertion"
        ]
    },
    "ECO:0000219": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-11-15T12:22:40Z"
        ],
        "def": [
            "\"A sequencing assay result for a biopolymer that comprises nucleotides.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000219"
        ],
        "is_a": [
            "ECO:0000220 ! sequencing assay evidence"
        ],
        "name": [
            "nucleotide sequencing assay evidence"
        ],
        "synonym": [
            "\"DNA sequencing\" RELATED [OBI:0000626]"
        ]
    },
    "ECO:0000220": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-11-15T01:44:45Z"
        ],
        "def": [
            "\"A type of experimental evidence where the order of molecules that constitute a biopolymer is determined.\" [ECO:MCC, OBI:0600047]"
        ],
        "id": [
            "ECO:0000220"
        ],
        "is_a": [
            "ECO:0000006 ! experimental evidence"
        ],
        "name": [
            "sequencing assay evidence"
        ]
    },
    "ECO:0000221": {
        "comment": [
            "Typically thousands to millions of reads are produced in parallel."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-11-15T02:19:34Z"
        ],
        "def": [
            "\"A nucleotide sequencing assay result that is derived from a parallelized sequencing technique.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000221"
        ],
        "is_a": [
            "ECO:0000219 ! nucleotide sequencing assay evidence"
        ],
        "name": [
            "high throughput nucleotide sequencing assay evidence"
        ]
    },
    "ECO:0000222": {
        "comment": [
            "In Illumina sequencing technology, adapters are ligated onto the ends of randomly fragmented DNA. After single-stranded fragments are bound to the inside surface of a flow cell channel, they undergo in-place bridge PCR amplification. To begin the sequencing cycle, labeled reversible terminators, primers and DNA polymerase are added, followed by laser excitation of the fluorescent label and high resolution scanning. After non-incorporated nucleotides are washed away and the dye is chemically removed, the next cycle repeats with four labeled reversible terminators, primers and DNA polymerase, again followed by imaging."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-11-15T02:38:03Z"
        ],
        "def": [
            "\"A high throughput nucleotide sequencing result derived by Illumina (Solexa) sequencing technology.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000222"
        ],
        "is_a": [
            "ECO:0000221 ! high throughput nucleotide sequencing assay evidence"
        ],
        "name": [
            "Illumina sequencing evidence"
        ],
        "synonym": [
            "\"Solexa sequencing result\" RELATED [OBI:0000724]"
        ]
    },
    "ECO:0000223": {
        "comment": [
            "454 pyrosequencing technology amplifies individual DNA fragments inside water droplets within an oil solution (emulsion PCR). Within each droplet, a single DNA template is attached to a single primer-coated bead. Each bead is subsequently captured in a picolitre well within the sequencing machine. A luciferase-based readout system is used to identify individual nucleotides added to the nascent DNA."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-11-15T03:50:14Z"
        ],
        "def": [
            "\"A high throughput nucleotide sequencing result derived by 454 pyrosequencing technology.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000223"
        ],
        "is_a": [
            "ECO:0000221 ! high throughput nucleotide sequencing assay evidence"
        ],
        "name": [
            "454 pyrosequencing evidence"
        ],
        "synonym": [
            "\"pyrosequencing\" BROAD [OBI:0000730]"
        ]
    },
    "ECO:0000224": {
        "comment": [
            "SOLiD sequencing technology is based on sequencing by ligation. After library preparation, emulsion PCR and bead enrichment are performed, including 3' modification of templates on selected beads to allow covalent bonding to a slide. 3' modified beads are attached to a glass slide and primers are added. Four fluorescently labeled di-base probes are added and multiple cycles of ligation, detection and cleavage are performed. The number of cycles determines read length. After a number of ligation cycles, the extension product is removed and another round of ligation cycles is performed with a new primer complimentary to the n-1 position of the template. Five rounds of primer reset are performed for each sequence tag."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-11-15T04:17:41Z"
        ],
        "def": [
            "\"A high throughput nucleotide sequencing result derived by SOLiD sequencing technology.\" [ECO:MCC, OBI:0000706]"
        ],
        "id": [
            "ECO:0000224"
        ],
        "is_a": [
            "ECO:0000221 ! high throughput nucleotide sequencing assay evidence"
        ],
        "name": [
            "SOLiD sequencing evidence"
        ]
    },
    "ECO:0000225": {
        "comment": [
            "Chain termination sequencing, also called Sanger sequencing after its developer, uses dideoxynucleotide triphosphates (ddNTPs) as DNA chain terminators. Single-stranded DNA template, DNA primer, DNA polymerase, fluorescently or radioactively labeled nucleotides, and one of four ddNTPs are added together in each of four separate sequencing reactions. The synthesized and labeled DNA fragments from each of the four reactions are denatured, separated by size by gel electrophoresis, and visualized by autoradiography or UV light."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-11-15T04:46:31Z"
        ],
        "def": [
            "\"A nucleotide sequencing assay result derived by the chain termination sequencing method.\" [ECO:MCC, OBI:0000695]"
        ],
        "id": [
            "ECO:0000225"
        ],
        "is_a": [
            "ECO:0000219 ! nucleotide sequencing assay evidence"
        ],
        "name": [
            "chain termination sequencing evidence"
        ],
        "synonym": [
            "\"dye terminator sequencing\" EXACT []",
            "\"Sanger sequencing\" EXACT []"
        ]
    },
    "ECO:0000226": {
        "comment": [
            "Chromatin immunoprecipitation (ChIP) is used to investigate protein-DNA interactions in vivo. DNA and proteins are chemically cross-linked in vivo to form chromatin complexes, followed by cell lysis and DNA shearing. Fragmented DNA-protein complexes are selectively enriched for a protein of interest using a specific antibody (immunoprecipitation). Protein digestion and DNA purification are performed prior to DNA detection via molecular cloning and sequencing, polymerase chain reaction (PCR), microarray analysis (ChIP-on-chip), or direct high-throughput sequencing (ChIP-seq)."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-11-16T01:29:08Z"
        ],
        "def": [
            "\"A type of immunoprecipitation evidence that is used to identify a protein binding site on a genomic DNA sequence.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000226"
        ],
        "is_a": [
            "ECO:0000085 ! immunoprecipitation evidence"
        ],
        "name": [
            "chromatin immunoprecipitation evidence"
        ],
        "synonym": [
            "\"ChIP evidence\" EXACT []"
        ],
        "xref": [
            "PSI-MI:MI\\:0402 \"chromatin immunoprecipitation assay\""
        ]
    },
    "ECO:0000227": {
        "comment": [
            "ChIP-PCR is often used to validate ChIP-chip results and less frequently,ChIP-Seq results."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-11-16T05:02:00Z"
        ],
        "def": [
            "\"A type of chromatin immunoprecipitation evidence that uses polymerase chain reaction (PCR) where specific primers are used on the pulled-down DNA for DNA detection.\" [ECO:MCC, ECO:SW, PMID:18388953]"
        ],
        "id": [
            "ECO:0000227"
        ],
        "is_a": [
            "ECO:0000226 ! chromatin immunoprecipitation evidence"
        ],
        "name": [
            "chromatin immunoprecipitation-PCR evidence"
        ],
        "synonym": [
            "\"ChIP-PCR evidence\" EXACT []"
        ]
    },
    "ECO:0000228": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-11-16T05:26:47Z"
        ],
        "def": [
            "\"A chromatin immunoprecipitation (ChIP) evidence that uses quantitative polymerase chain reaction (qPCR) for DNA detection.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000228"
        ],
        "is_a": [
            "ECO:0000226 ! chromatin immunoprecipitation evidence"
        ],
        "name": [
            "chromatin immunoprecipitation-qPCR evidence"
        ],
        "synonym": [
            "\"ChIP-qPCR evidence\" EXACT []"
        ]
    },
    "ECO:0000229": {
        "comment": [
            "ChIP-Seq experiments often use the input as a control to eliminate biases. To obtain similar results, ChIP-chip with high-density tiling arrays can be used."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-11-16T05:30:41Z"
        ],
        "def": [
            "\"A type of chromatin immunoprecipitation evidence that uses high-throughput sequencing where immunoprecipitated DNA fragments are funnelled into a massively parallel sequencer to produce multiple short reads for locating binding sites of DNA-associated proteins.\" [ECO:MCC, ECO:SW, OBI:0000716, PMID:22955991]"
        ],
        "id": [
            "ECO:0000229"
        ],
        "is_a": [
            "ECO:0000226 ! chromatin immunoprecipitation evidence"
        ],
        "name": [
            "chromatin immunoprecipitation-seq evidence"
        ],
        "synonym": [
            "\"ChIP-SEQ evidence\" EXACT [OBI:0000716]",
            "\"ChIP-seq evidence\" EXACT [PMID:22955991]"
        ]
    },
    "ECO:0000230": {
        "comment": [
            "A fixating agent such as formaldehyde is used to cross link proteins and DNA. Sonication is applied for DNA shearing. For transcription factor tagging, an antibody or epitope is employed. After cross-linking is reversed, the DNA is amplified and labeled with a flourophore for microarray which produces a resolution of aproximately 500 bp."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-11-16T05:43:55Z"
        ],
        "def": [
            "\"A type of chromatin immunoprecipitation evidence that uses a tiling microarray for the detection of protein-bound DNA regions where chromatin is immunoprecipitated for protein tagging and DNA is sheared from the cross-linked protein-DNA complex to be arrayed revealing the protein-bound genomic regions.\" [ECO:MCC, ECO:SW, PMID:18388953, PMID:19381927]"
        ],
        "id": [
            "ECO:0000230"
        ],
        "is_a": [
            "ECO:0000226 ! chromatin immunoprecipitation evidence"
        ],
        "name": [
            "chromatin immunoprecipitation-chip evidence"
        ],
        "synonym": [
            "\"ChIP-chip evidence\" EXACT []",
            "\"ChIP-on-chip evidence\" EXACT []"
        ],
        "xref": [
            "PSI-MI:MI\\:0225 \"chromatin immunoprecipitation array\""
        ]
    },
    "ECO:0000231": {
        "comment": [
            "Quantitative PCR enables both detection and quantification (as absolute number of copies or relative amount when normalized to DNA input or additional normalizing genes) of one or more specific sequences in a DNA sample. The dye is activated upon binding double-stranded DNA.\\nAlthough qRT-PCR is commonly found in literature synonymous to qPCR, it is preferable not to use this synonym as it is the accepted abbreviation for reverse transcription PCR. qPCR should be used instead (ISBN 978-0-470-68386-6 page 173)."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-11-16T05:57:20Z"
        ],
        "def": [
            "\"A type of polymerase chain reaction evidence where the reaction product is quantified in real-time across cycles by means of fluorescent dyes for detection and quantification of specific sequences in a DNA sample.\" [ECO:MCC, ECO:SW]"
        ],
        "id": [
            "ECO:0000231"
        ],
        "is_a": [
            "ECO:0000082 ! polymerase chain reaction evidence"
        ],
        "name": [
            "quantitative polymerase chain reaction evidence"
        ],
        "synonym": [
            "\"Q-PCR evidence\" EXACT []",
            "\"qRT-PCR evidence\" RELATED []",
            "\"quantitative PCR evidence\" EXACT []",
            "\"real-time PCR evidence\" EXACT []",
            "\"real-time polymerase chain reaction evidence\" EXACT []"
        ]
    },
    "ECO:0000232": {
        "comment": [
            "Chromosome conformation capture technologies are used to study the structural properties and spatial organization of chromosomes inside cells. All types of chromosome conformation capture technology involve five primary steps: cross-linking of DNA in vivo, restriction digestion, intramolecular ligation, reversing cross-linkages, and DNA enrichment and quantitation. The last step is different for each type of chromosome conformation capture technology, and may include PCR (3C), inverse PCR (4C), or ligation-mediated amplification (5C). Methods for product quantitation can include agarose gel detection or real-time quantitative PCR in the case of 3C, or high-throughput sequencing or microarray analysis for 4C and 5C."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-11-17T12:27:14Z"
        ],
        "def": [
            "\"A type of experimental evidence that is based on a five-step technique of cross-linking DNA in vivo, restriction digestion, intramolecular ligation, reversing DNA cross-links, and DNA processing and quantitation.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000232"
        ],
        "is_a": [
            "ECO:0000073 ! experimental genomic evidence"
        ],
        "name": [
            "chromosome conformation capture-based evidence"
        ],
        "synonym": [
            "\"3C\" NARROW []",
            "\"chromosome conformation capture\" NARROW []"
        ]
    },
    "ECO:0000233": {
        "comment": [
            "3C is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 3C differs only in the fifth step, which involves performing either standard polymerase chain reaction (PCR) or quantitative PCR (qPCR) for product quantitation."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-11-17T12:46:15Z"
        ],
        "def": [
            "\"A type of chromosome conformation capture-based evidence that is derived by performing a type of polymerase chain reaction (PCR) in the final product quantitation step.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000233"
        ],
        "is_a": [
            "ECO:0000232 ! chromosome conformation capture-based evidence"
        ],
        "name": [
            "chromosome conformation capture evidence"
        ],
        "synonym": [
            "\"3C\" EXACT []"
        ]
    },
    "ECO:0000234": {
        "comment": [
            "4C is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 4C differs only in the fifth step, which involves inverse polymerase chain reaction (inverse PCR) on a circularized 3C library prior to product quantitation."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-11-19T01:20:28Z"
        ],
        "def": [
            "\"A type of chromosome conformation capture-based evidence that is derived by inverse polymerase chain reaction (inverse PCR) on a prior circularized 3C library followed by product quantitation with a microarray.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000234"
        ],
        "is_a": [
            "ECO:0000232 ! chromosome conformation capture-based evidence"
        ],
        "name": [
            "circularized chromosome conformation capture evidence"
        ],
        "synonym": [
            "\"4C\" EXACT []",
            "\"chromosome conformation capture on chip\" EXACT []",
            "\"circularized 3C\" EXACT []"
        ]
    },
    "ECO:0000235": {
        "comment": [
            "5C is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 5C differs only in the fifth step, which involves ligation-mediated amplification (LMA) followed by product quantitation."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-11-19T01:22:20Z"
        ],
        "def": [
            "\"A type of chromosome conformation capture-based evidence that is derived by performing ligation-mediated amplification (LMA) prior to product quantitation.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000235"
        ],
        "is_a": [
            "ECO:0000232 ! chromosome conformation capture-based evidence"
        ],
        "name": [
            "carbon-copy chromosome conformation capture evidence"
        ],
        "synonym": [
            "\"5C\" EXACT []",
            "\"carbon-copy 3C\" EXACT []"
        ]
    },
    "ECO:0000236": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-11-19T03:00:47Z"
        ],
        "def": [
            "\"A type of chromosome conformation capture evidence that is derived by performing polymerase chain reaction (PCR) in the quantitation step.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000236"
        ],
        "is_a": [
            "ECO:0000233 ! chromosome conformation capture evidence"
        ],
        "name": [
            "chromosome conformation capture-PCR evidence"
        ],
        "synonym": [
            "\"3C\" BROAD []",
            "\"3C-PCR\" EXACT []"
        ]
    },
    "ECO:0000237": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-11-19T03:06:47Z"
        ],
        "def": [
            "\"A type of chromosome conformation capture evidence that is derived by performing quantitative polymerase chain reaction (qPCR) in the quantitation step.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000237"
        ],
        "is_a": [
            "ECO:0000233 ! chromosome conformation capture evidence"
        ],
        "name": [
            "chromosome conformation capture-qPCR evidence"
        ],
        "synonym": [
            "\"3C-qPCR\" EXACT []",
            "\"3C-quantitative PCR\" EXACT []",
            "\"3C-real-time PCR\" EXACT []"
        ]
    },
    "ECO:0000238": {
        "comment": [
            "Hi-C is used to study the structural properties and spatial organization of chromosomes inside cells. Hi-C is similar to other types of chromosome conformation capture technology, but involves the addition of biotinylated nucleotides during the second or fourth step and enrichment for ligated fragments using immunoprecipitation after the fourth step."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-11-19T04:24:03Z"
        ],
        "def": [
            "\"A type of chromosome conformation capture-based evidence that is derived by performing immunoprecipitation enrichment of ligated fragments events prior to product quantitation.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000238"
        ],
        "is_a": [
            "ECO:0000232 ! chromosome conformation capture-based evidence"
        ],
        "name": [
            "Hi-C evidence"
        ]
    },
    "ECO:0000239": {
        "comment": [
            "3C-seq is used to study the structural properties and spatial organization of chromosomes inside cells. Like other types of chromosome conformation capture technology, 3C-seq differs only in the fifth step, which involves inverse polymerase chain reaction (PCR) on a circularized 3C library prior to product quantitation."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-11-30T03:22:25Z"
        ],
        "def": [
            "\"A type of chromosome conformation capture-based evidence that is derived by inverse polymerase chain reaction (inverse PCR) on a circularized 3C library prior to product quantitation with high-throughput (HT) sequencing.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000239"
        ],
        "is_a": [
            "ECO:0000232 ! chromosome conformation capture-based evidence"
        ],
        "name": [
            "chromosome conformation capture sequencing evidence"
        ],
        "synonym": [
            "\"3C sequencing\" EXACT []",
            "\"3C-seq\" EXACT []",
            "\"4C sequencing\" EXACT []",
            "\"4C-seq\" EXACT []",
            "\"circularized 3C sequencing\" EXACT []",
            "\"circularized 3C-seq\" EXACT []"
        ]
    },
    "ECO:0000240": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-06T12:24:16Z"
        ],
        "def": [
            "\"Experimental phenotypic evidence where a structural feature of an organism is disrupted.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000240"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "anatomical perturbation evidence"
        ]
    },
    "ECO:0000241": {
        "comment": [
            "Many environmental factors are subject to modification, such as temperature, pressure, humidity, radiation, and so forth."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-06T12:33:58Z"
        ],
        "def": [
            "\"Experimental phenotypic evidence where the extra-organismal context of an organism is modified.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000241"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "environmental perturbation evidence"
        ],
        "synonym": [
            "\"mechanical constraint evidence\" RELATED []"
        ]
    },
    "ECO:0000242": {
        "comment": [
            "A tissue ablation experiment involves observing the phenotype of an entity whose tissues have been disrupted through genetic or biochemical manipulation, physical destruction, or some other means."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-06T12:33:58Z"
        ],
        "def": [
            "\"A type of anatomical perturbation that is based on disruption of tissue.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000242"
        ],
        "is_a": [
            "ECO:0000240 ! anatomical perturbation evidence"
        ],
        "name": [
            "tissue ablation evidence"
        ],
        "synonym": [
            "\"ablated tissue evidence\" EXACT []"
        ]
    },
    "ECO:0000243": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-06T12:33:58Z"
        ],
        "def": [
            "\"A type of anatomical perturbation that is based on adding tissue to an organism through a graft procedure.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000243"
        ],
        "is_a": [
            "ECO:0000240 ! anatomical perturbation evidence"
        ],
        "name": [
            "tissue grafting evidence"
        ]
    },
    "ECO:0000244": {
        "comment": [
            "Combinatorial analyses could include experimental or computational results. Examples include: (i) large-scale experiment such as a genome-wide two-hybrid or genome-wide synthetic interactions; (ii) integration of large-scale data sets of various types; and (iii) text-based-computation, e.g. text-mining. For simple sequence comparisons, one should use the sequence similarity analysis evidence type. For microarray results alone, expression pattern analysis is appropriate; whereas, large-scale computational analysis should be used when microarray results are combined with the results of other types of large-scale experiments."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-09T02:02:12Z"
        ],
        "def": [
            "\"A type of combinatorial analysis that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000244"
        ],
        "intersection_of": [
            "ECO:0000212 ! combinatorial evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000212 ! combinatorial evidence",
            "ECO:0000352 {is_inferred=\"true\"} ! evidence used in manual assertion"
        ],
        "name": [
            "combinatorial evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000245": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-09T02:37:54Z"
        ],
        "def": [
            "\"A type of computational combinatorial analysis that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000245"
        ],
        "intersection_of": [
            "ECO:0000053 ! computational combinatorial evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000053 ! computational combinatorial evidence",
            "ECO:0000244 {is_inferred=\"true\"} ! combinatorial evidence used in manual assertion"
        ],
        "name": [
            "computational combinatorial evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ],
        "subset": [
            "valid_with_gene",
            "valid_with_protein",
            "valid_with_protein_complex"
        ],
        "synonym": [
            "\"inferred from reviewed computational analysis\" EXACT [GOECO:RCA]",
            "\"RCA\" EXACT [GOECO:RCA]"
        ],
        "xref": [
            "GOECO:RCA \"inferred from reviewed computational analysis\""
        ]
    },
    "ECO:0000246": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-09T02:37:54Z"
        ],
        "def": [
            "\"A type of computational combinatorial analysis that is used in an automatic assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000246"
        ],
        "intersection_of": [
            "ECO:0000053 ! computational combinatorial evidence",
            "used_in ECO:0000203 ! automatic assertion"
        ],
        "is_a": [
            "ECO:0000053 ! computational combinatorial evidence",
            "ECO:0000213 {is_inferred=\"true\"} ! combinatorial evidence used in automatic assertion"
        ],
        "name": [
            "computational combinatorial evidence used in automatic assertion"
        ],
        "relationship": [
            "used_in ECO:0000203 {is_inferred=\"true\"} ! automatic assertion"
        ]
    },
    "ECO:0000247": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-09T05:12:27Z"
        ],
        "def": [
            "\"Sequence alignment evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000247"
        ],
        "intersection_of": [
            "ECO:0000200 ! sequence alignment evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000200 ! sequence alignment evidence",
            "ECO:0000250 {is_inferred=\"true\"} ! sequence similarity evidence used in manual assertion"
        ],
        "name": [
            "sequence alignment evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ],
        "subset": [
            "valid_with_gene",
            "valid_with_protein"
        ],
        "synonym": [
            "\"inferred from sequence alignment\" EXACT [GOECO:ISA]",
            "\"ISA\" EXACT [GOECO:ISA]"
        ],
        "xref": [
            "GOECO:ISA \"inferred from sequence alignment\""
        ]
    },
    "ECO:0000248": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-09T05:12:27Z"
        ],
        "def": [
            "\"Sequence alignment evidence that is used in an automatic assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000248"
        ],
        "intersection_of": [
            "ECO:0000200 ! sequence alignment evidence",
            "used_in ECO:0000203 ! automatic assertion"
        ],
        "is_a": [
            "ECO:0000200 ! sequence alignment evidence",
            "ECO:0000249 {is_inferred=\"true\"} ! sequence similarity evidence used in automatic assertion"
        ],
        "name": [
            "sequence alignment evidence used in automatic assertion"
        ],
        "relationship": [
            "used_in ECO:0000203 {is_inferred=\"true\"} ! automatic assertion"
        ]
    },
    "ECO:0000249": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-09T05:22:30Z"
        ],
        "def": [
            "\"Sequence similarity evidence that is used in an automatic assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000249"
        ],
        "intersection_of": [
            "ECO:0000044 ! sequence similarity evidence",
            "used_in ECO:0000203 ! automatic assertion"
        ],
        "is_a": [
            "ECO:0000044 ! sequence similarity evidence",
            "ECO:0000251 {is_inferred=\"true\"} ! similarity evidence used in automatic assertion"
        ],
        "name": [
            "sequence similarity evidence used in automatic assertion"
        ],
        "relationship": [
            "used_in ECO:0000203 {is_inferred=\"true\"} ! automatic assertion"
        ]
    },
    "ECO:0000250": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-09T05:22:30Z"
        ],
        "def": [
            "\"Sequence similarity evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000250"
        ],
        "intersection_of": [
            "ECO:0000044 ! sequence similarity evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000044 ! sequence similarity evidence",
            "ECO:0000252 {is_inferred=\"true\"} ! similarity evidence used in manual assertion"
        ],
        "name": [
            "sequence similarity evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ],
        "subset": [
            "valid_with_gene",
            "valid_with_protein",
            "valid_with_protein_complex"
        ],
        "xref": [
            "GOECO:ISS \"inferred from sequence or structural similarity\""
        ]
    },
    "ECO:0000251": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-09T05:28:12Z"
        ],
        "def": [
            "\"Similarity evidence that is used in an automatic assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000251"
        ],
        "intersection_of": [
            "ECO:0000041 ! similarity evidence",
            "used_in ECO:0000203 ! automatic assertion"
        ],
        "is_a": [
            "ECO:0000041 ! similarity evidence",
            "ECO:0000501 {is_inferred=\"true\"} ! evidence used in automatic assertion"
        ],
        "name": [
            "similarity evidence used in automatic assertion"
        ],
        "relationship": [
            "used_in ECO:0000203 {is_inferred=\"true\"} ! automatic assertion"
        ]
    },
    "ECO:0000252": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-09T05:28:12Z"
        ],
        "def": [
            "\"Similarity evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000252"
        ],
        "intersection_of": [
            "ECO:0000041 ! similarity evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000041 ! similarity evidence",
            "ECO:0000352 {is_inferred=\"true\"} ! evidence used in manual assertion"
        ],
        "name": [
            "similarity evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ],
        "subset": [
            "go_groupings"
        ]
    },
    "ECO:0000253": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-10T01:42:44Z"
        ],
        "def": [
            "\"Genetic similarity evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000253"
        ],
        "intersection_of": [
            "ECO:0000051 ! genetic similarity evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000051 ! genetic similarity evidence",
            "ECO:0000252 {is_inferred=\"true\"} ! similarity evidence used in manual assertion"
        ],
        "name": [
            "genetic similarity evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000254": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-10T01:42:44Z"
        ],
        "def": [
            "\"Genetic similarity evidence that is used in an automatic assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000254"
        ],
        "intersection_of": [
            "ECO:0000051 ! genetic similarity evidence",
            "used_in ECO:0000203 ! automatic assertion"
        ],
        "is_a": [
            "ECO:0000051 ! genetic similarity evidence",
            "ECO:0000251 {is_inferred=\"true\"} ! similarity evidence used in automatic assertion"
        ],
        "name": [
            "genetic similarity evidence used in automatic assertion"
        ],
        "relationship": [
            "used_in ECO:0000203 {is_inferred=\"true\"} ! automatic assertion"
        ]
    },
    "ECO:0000255": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-10T02:26:35Z"
        ],
        "def": [
            "\"A match to sequence model evidence that is employed in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000255"
        ],
        "intersection_of": [
            "ECO:0000202 ! match to sequence model evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000202 ! match to sequence model evidence",
            "ECO:0000250 {is_inferred=\"true\"} ! sequence similarity evidence used in manual assertion"
        ],
        "name": [
            "match to sequence model evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ],
        "xref": [
            "GO_REF:0000011 \"Hidden Markov Models (TIGR)\"",
            "GOECO:ISM \"inferred from sequence model\""
        ]
    },
    "ECO:0000256": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-10T02:26:35Z"
        ],
        "def": [
            "\"A match to sequence model evidence that is employed in an automatic assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000256"
        ],
        "intersection_of": [
            "ECO:0000202 ! match to sequence model evidence",
            "used_in ECO:0000203 ! automatic assertion"
        ],
        "is_a": [
            "ECO:0000202 ! match to sequence model evidence",
            "ECO:0000249 {is_inferred=\"true\"} ! sequence similarity evidence used in automatic assertion"
        ],
        "name": [
            "match to sequence model evidence used in automatic assertion"
        ],
        "relationship": [
            "used_in ECO:0000203 {is_inferred=\"true\"} ! automatic assertion"
        ],
        "xref": [
            "GO_REF:0000002 \"Gene Ontology annotation through association of InterPro records with GO terms.\"",
            "GO_REF:0000019 \"Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara\"",
            "GO_REF:0000107 \"Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl\""
        ]
    },
    "ECO:0000257": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-10T02:44:34Z"
        ],
        "def": [
            "\"Motif similarity evidence that is employed in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000257"
        ],
        "intersection_of": [
            "ECO:0000028 ! motif similarity evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000028 ! motif similarity evidence",
            "ECO:0000255 {is_inferred=\"true\"} ! match to sequence model evidence used in manual assertion"
        ],
        "name": [
            "motif similarity evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000258": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-10T02:44:34Z"
        ],
        "def": [
            "\"Motif similarity evidence that is employed in an automatic assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000258"
        ],
        "intersection_of": [
            "ECO:0000028 ! motif similarity evidence",
            "used_in ECO:0000203 ! automatic assertion"
        ],
        "is_a": [
            "ECO:0000028 ! motif similarity evidence",
            "ECO:0000256 {is_inferred=\"true\"} ! match to sequence model evidence used in automatic assertion"
        ],
        "name": [
            "motif similarity evidence used in automatic assertion"
        ],
        "relationship": [
            "used_in ECO:0000203 {is_inferred=\"true\"} ! automatic assertion"
        ]
    },
    "ECO:0000259": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-10T02:54:51Z"
        ],
        "def": [
            "\"A match to InterPro member signature evidence that is employed in an automatic assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000259"
        ],
        "intersection_of": [
            "ECO:0000029 ! match to InterPro member signature evidence",
            "used_in ECO:0000203 ! automatic assertion"
        ],
        "is_a": [
            "ECO:0000029 ! match to InterPro member signature evidence",
            "ECO:0000256 {is_inferred=\"true\"} ! match to sequence model evidence used in automatic assertion"
        ],
        "name": [
            "match to InterPro member signature evidence used in automatic assertion"
        ],
        "relationship": [
            "used_in ECO:0000203 {is_inferred=\"true\"} ! automatic assertion"
        ]
    },
    "ECO:0000260": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-10T02:54:51Z"
        ],
        "def": [
            "\"A match to InterPro member signature evidence that is employed in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000260"
        ],
        "intersection_of": [
            "ECO:0000029 ! match to InterPro member signature evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000029 ! match to InterPro member signature evidence",
            "ECO:0000255 {is_inferred=\"true\"} ! match to sequence model evidence used in manual assertion"
        ],
        "name": [
            "match to InterPro member signature evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000261": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-10T03:02:49Z"
        ],
        "def": [
            "\"A targeting sequence prediction that is employed in an automatic assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000261"
        ],
        "intersection_of": [
            "ECO:0000081 ! targeting sequence prediction evidence",
            "used_in ECO:0000203 ! automatic assertion"
        ],
        "is_a": [
            "ECO:0000081 ! targeting sequence prediction evidence",
            "ECO:0000258 {is_inferred=\"true\"} ! motif similarity evidence used in automatic assertion"
        ],
        "name": [
            "targeting sequence prediction evidence used in automatic assertion"
        ],
        "relationship": [
            "used_in ECO:0000203 {is_inferred=\"true\"} ! automatic assertion"
        ]
    },
    "ECO:0000262": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-10T03:02:49Z"
        ],
        "def": [
            "\"A targeting sequence prediction that is employed in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000262"
        ],
        "intersection_of": [
            "ECO:0000081 ! targeting sequence prediction evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000081 ! targeting sequence prediction evidence",
            "ECO:0000257 {is_inferred=\"true\"} ! motif similarity evidence used in manual assertion"
        ],
        "name": [
            "targeting sequence prediction evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000263": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-10T03:28:35Z"
        ],
        "def": [
            "\"Transmembrane domain prediction that is employed in an automatic assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000263"
        ],
        "intersection_of": [
            "ECO:0000083 ! transmembrane domain prediction evidence",
            "used_in ECO:0000203 ! automatic assertion"
        ],
        "is_a": [
            "ECO:0000083 ! transmembrane domain prediction evidence",
            "ECO:0000258 {is_inferred=\"true\"} ! motif similarity evidence used in automatic assertion"
        ],
        "name": [
            "transmembrane domain prediction evidence used in automatic assertion"
        ],
        "relationship": [
            "used_in ECO:0000203 {is_inferred=\"true\"} ! automatic assertion"
        ]
    },
    "ECO:0000264": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-10T03:28:35Z"
        ],
        "def": [
            "\"Transmembrane domain prediction that is employed in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000264"
        ],
        "intersection_of": [
            "ECO:0000083 ! transmembrane domain prediction evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000083 ! transmembrane domain prediction evidence",
            "ECO:0000257 {is_inferred=\"true\"} ! motif similarity evidence used in manual assertion"
        ],
        "name": [
            "transmembrane domain prediction evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000265": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-10T03:39:41Z"
        ],
        "def": [
            "\"Sequence orthology evidence that is employed in an automatic assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000265"
        ],
        "intersection_of": [
            "ECO:0000201 ! sequence orthology evidence",
            "used_in ECO:0000203 ! automatic assertion"
        ],
        "is_a": [
            "ECO:0000201 ! sequence orthology evidence",
            "ECO:0000249 {is_inferred=\"true\"} ! sequence similarity evidence used in automatic assertion"
        ],
        "name": [
            "sequence orthology evidence used in automatic assertion"
        ],
        "relationship": [
            "used_in ECO:0000203 {is_inferred=\"true\"} ! automatic assertion"
        ],
        "synonym": [
            "\"Ortholog evidence\" RELATED []"
        ],
        "xref": [
            "GO_REF:0000003 \"Gene Ontology annotation based on Enzyme Commission mapping.\"",
            "GO_REF:0000019 \"Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara\"",
            "GO_REF:0000035 \"Automatic transfer of experimentally verified manual GO annotation data to plant orthologs using Ensembl Compara\""
        ]
    },
    "ECO:0000266": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-10T03:39:41Z"
        ],
        "def": [
            "\"Sequence orthology evidence that is employed in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000266"
        ],
        "intersection_of": [
            "ECO:0000201 ! sequence orthology evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000201 ! sequence orthology evidence",
            "ECO:0000250 {is_inferred=\"true\"} ! sequence similarity evidence used in manual assertion"
        ],
        "name": [
            "sequence orthology evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ],
        "subset": [
            "valid_with_gene",
            "valid_with_protein",
            "valid_with_protein_complex"
        ],
        "synonym": [
            "\"Ortholog evidence\" RELATED []"
        ],
        "xref": [
            "GOECO:ISO \"inferred from sequence orthology\""
        ]
    },
    "ECO:0000267": {
        "comment": [
            "ELISA is sometimes used in the study of transcriptional regulation to identify a produced protein, especially secreted protein."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-14T01:57:13Z"
        ],
        "def": [
            "\"A type of protein detection assay evidence where an antigen is bound to a substrate, and an antibody directly or indirectly linked to an enzyme is utilized to determine the amount of bound antigen which is visualized with a color change in the substrate.\" [ECO:MCC, ECO:SW, PMID:23949770]"
        ],
        "id": [
            "ECO:0000267"
        ],
        "is_a": [
            "ECO:0005517 ! protein detection assay evidence"
        ],
        "name": [
            "enzyme-linked immunoabsorbent assay evidence"
        ],
        "synonym": [
            "\"ELISA evidence\" EXACT []",
            "\"enzyme-linked immunosorbent assay\" NARROW []"
        ],
        "xref": [
            "PSI-MI:MI\\:0411 \"enzyme linked immunosorbent assay\""
        ]
    },
    "ECO:0000268": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-14T02:07:08Z"
        ],
        "def": [
            "\"A direct assay that passes a stream of cells or particles past a light source, followed by using a detector to monitor light scatter or fluorochrome excitation to characterize the cells or particles.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000268"
        ],
        "is_a": [
            "ECO:0000002 ! direct assay evidence"
        ],
        "name": [
            "flow cytometry evidence"
        ],
        "synonym": [
            "\"FCM\" EXACT []"
        ]
    },
    "ECO:0000269": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-20T05:36:49Z"
        ],
        "def": [
            "\"A type of experimental evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000269"
        ],
        "intersection_of": [
            "ECO:0000006 ! experimental evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000006 ! experimental evidence",
            "ECO:0000352 {is_inferred=\"true\"} ! evidence used in manual assertion"
        ],
        "name": [
            "experimental evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ],
        "xref": [
            "GOECO:EXP \"inferred from experiment\""
        ]
    },
    "ECO:0000270": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-20T05:46:39Z"
        ],
        "def": [
            "\"A type of expression pattern evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000270"
        ],
        "intersection_of": [
            "ECO:0000008 ! expression pattern evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000008 ! expression pattern evidence",
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion"
        ],
        "name": [
            "expression evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ],
        "xref": [
            "GOECO:IEP \"inferred from expression pattern\""
        ]
    },
    "ECO:0000271": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-20T05:51:58Z"
        ],
        "def": [
            "\"A type of array experiment evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000271"
        ],
        "intersection_of": [
            "ECO:0000055 ! array experiment evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000055 ! array experiment evidence",
            "ECO:0000270 {is_inferred=\"true\"} ! expression evidence used in manual assertion"
        ],
        "name": [
            "array experiment evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000272": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-20T05:53:36Z"
        ],
        "def": [
            "\"A type of Affymetrix array experiment evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000272"
        ],
        "intersection_of": [
            "ECO:0000101 ! Affymetrix array experiment evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000101 ! Affymetrix array experiment evidence",
            "ECO:0000271 {is_inferred=\"true\"} ! array experiment evidence used in manual assertion"
        ],
        "name": [
            "Affymetrix array experiment evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000273": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-20T05:55:49Z"
        ],
        "def": [
            "\"A type of evidence arising from a cDNA microarray experiment that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000273"
        ],
        "intersection_of": [
            "ECO:0000097 ! cDNA microarray evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000097 ! cDNA microarray evidence",
            "ECO:0000271 {is_inferred=\"true\"} ! array experiment evidence used in manual assertion"
        ],
        "name": [
            "cDNA microarray evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000274": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-20T05:58:44Z"
        ],
        "def": [
            "\"A type of evidence arising from a CpG island microarray experiment that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000274"
        ],
        "intersection_of": [
            "ECO:0000065 ! CpG island microarray evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000065 ! CpG island microarray evidence",
            "ECO:0000271 {is_inferred=\"true\"} ! array experiment evidence used in manual assertion"
        ],
        "name": [
            "CpG island microarray evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000275": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-20T06:01:26Z"
        ],
        "def": [
            "\"A type of expression microarray evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000275"
        ],
        "intersection_of": [
            "ECO:0000058 ! expression microarray evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000058 ! expression microarray evidence",
            "ECO:0000271 {is_inferred=\"true\"} ! array experiment evidence used in manual assertion"
        ],
        "name": [
            "expression microarray evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000276": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-20T06:03:28Z"
        ],
        "def": [
            "\"A type of evidence arising from a genomic microarray experiment that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000276"
        ],
        "intersection_of": [
            "ECO:0000062 ! genomic microarray evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000062 ! genomic microarray evidence",
            "ECO:0000271 {is_inferred=\"true\"} ! array experiment evidence used in manual assertion"
        ],
        "name": [
            "genomic microarray evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000277": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-20T06:05:20Z"
        ],
        "def": [
            "\"A type of evidence arising from a Nimblegen array experiment that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000277"
        ],
        "intersection_of": [
            "ECO:0000105 ! Nimblegen array evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000105 ! Nimblegen array evidence",
            "ECO:0000271 {is_inferred=\"true\"} ! array experiment evidence used in manual assertion"
        ],
        "name": [
            "Nimblegen array evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000278": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-20T06:09:45Z"
        ],
        "def": [
            "\"A type of evidence arising from an oligonucleotide microarray experiment that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000278"
        ],
        "intersection_of": [
            "ECO:0000093 ! oligonucleotide microarray evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000093 ! oligonucleotide microarray evidence",
            "ECO:0000271 {is_inferred=\"true\"} ! array experiment evidence used in manual assertion"
        ],
        "name": [
            "oligonucleotide microarray evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000279": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-20T06:20:01Z"
        ],
        "def": [
            "\"A type of Western blot assay that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000279"
        ],
        "intersection_of": [
            "ECO:0000112 ! western blot evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000112 ! western blot evidence",
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion"
        ],
        "name": [
            "western blot evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000280": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-20T06:21:54Z"
        ],
        "def": [
            "\"A type of protein expression level analysis that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000280"
        ],
        "intersection_of": [
            "ECO:0000046 ! protein expression level evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000046 ! protein expression level evidence",
            "ECO:0000284 {is_inferred=\"true\"} ! protein expression evidence used in manual assertion"
        ],
        "name": [
            "protein expression level evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000281": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-20T06:24:05Z"
        ],
        "def": [
            "\"A type of expression library screen evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000281"
        ],
        "intersection_of": [
            "ECO:0000114 ! expression library screen evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000114 ! expression library screen evidence",
            "ECO:0000284 {is_inferred=\"true\"} ! protein expression evidence used in manual assertion"
        ],
        "name": [
            "expression library screen evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000282": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-20T06:27:04Z"
        ],
        "def": [
            "\"A type of heterologous protein expression analysis that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000282"
        ],
        "intersection_of": [
            "ECO:0000154 ! heterologous protein expression evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000154 ! heterologous protein expression evidence",
            "ECO:0000284 {is_inferred=\"true\"} ! protein expression evidence used in manual assertion"
        ],
        "name": [
            "heterologous protein expression evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000283": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-20T06:31:44Z"
        ],
        "def": [
            "\"A type of protein expression spatial pattern analysis that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000283"
        ],
        "intersection_of": [
            "ECO:0000045 ! spatial pattern of protein expression evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000045 ! spatial pattern of protein expression evidence",
            "ECO:0000284 {is_inferred=\"true\"} ! protein expression evidence used in manual assertion"
        ],
        "name": [
            "spatial pattern of protein expression evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000284": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-20T06:33:46Z"
        ],
        "def": [
            "\"A type of protein expression analysis that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000284"
        ],
        "intersection_of": [
            "ECO:0000010 ! protein expression evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000010 ! protein expression evidence",
            "ECO:0000270 {is_inferred=\"true\"} ! expression evidence used in manual assertion",
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion"
        ],
        "name": [
            "protein expression evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000285": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-20T06:41:33Z"
        ],
        "def": [
            "\"A type of microarray RNA expression level data that is used in manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000285"
        ],
        "intersection_of": [
            "ECO:0000104 ! microarray RNA expression level evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000104 ! microarray RNA expression level evidence",
            "ECO:0000275 {is_inferred=\"true\"} ! expression microarray evidence used in manual assertion",
            "ECO:0000286 {is_inferred=\"true\"} ! transcript expression level evidence used in manual assertion"
        ],
        "name": [
            "microarray RNA expression level evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000286": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-20T06:52:03Z"
        ],
        "def": [
            "\"A type of transcript expression level data that is used in manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000286"
        ],
        "intersection_of": [
            "ECO:0000048 ! transcript expression level evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000048 ! transcript expression level evidence",
            "ECO:0000291 {is_inferred=\"true\"} ! transcript expression evidence used in manual assertion"
        ],
        "name": [
            "transcript expression level evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000287": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-20T06:55:14Z"
        ],
        "def": [
            "\"A type of differential hybridization experiment that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000287"
        ],
        "intersection_of": [
            "ECO:0000116 ! differential hybridization evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000116 ! differential hybridization evidence",
            "ECO:0000291 {is_inferred=\"true\"} ! transcript expression evidence used in manual assertion"
        ],
        "name": [
            "differential hybridization evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000288": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-20T06:57:33Z"
        ],
        "def": [
            "\"A type of RNA protection assay that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000288"
        ],
        "intersection_of": [
            "ECO:0000110 ! RNA protection assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000110 ! RNA protection assay evidence",
            "ECO:0001240 {is_inferred=\"true\"} ! nuclease protection assay evidence used in manual assertion"
        ],
        "name": [
            "RNA protection assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000289": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-20T06:59:18Z"
        ],
        "def": [
            "\"A type of spatial pattern of transcript expression analysis that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000289"
        ],
        "intersection_of": [
            "ECO:0000047 ! spatial pattern of transcript expression evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000047 ! spatial pattern of transcript expression evidence",
            "ECO:0000291 {is_inferred=\"true\"} ! transcript expression evidence used in manual assertion"
        ],
        "name": [
            "spatial pattern of transcript expression evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000290": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-20T07:00:48Z"
        ],
        "def": [
            "\"A type of subtractive hybridization experiment that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000290"
        ],
        "intersection_of": [
            "ECO:0000118 ! subtractive hybridization evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000118 ! subtractive hybridization evidence",
            "ECO:0000291 {is_inferred=\"true\"} ! transcript expression evidence used in manual assertion"
        ],
        "name": [
            "subtractive hybridization evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000291": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2010-12-20T07:02:08Z"
        ],
        "def": [
            "\"A type of transcript expression evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000291"
        ],
        "intersection_of": [
            "ECO:0000009 ! transcript expression evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000009 ! transcript expression evidence",
            "ECO:0000270 {is_inferred=\"true\"} ! expression evidence used in manual assertion"
        ],
        "name": [
            "transcript expression evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000292": {
        "comment": [
            "Morpholino oligonucleotides modify gene expression by blocking access of other molecules to specific, approximately 25-base-long regions of the base-pairing surfaces of ribonucleic acid (RNA). To achieve this anti-sense knockdown technique, delivery of the morpholino can be achieved via microinjection, electroporation, or another method."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-01-10T03:36:25Z"
        ],
        "def": [
            "\"A type of experimental phenotypic evidence that uses anti-sense by introducing morpholino oligonucleotides into the cytosol of a cell.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000292"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "morpholino experiment evidence"
        ],
        "synonym": [
            "\"anti-sense\" BROAD []"
        ]
    },
    "ECO:0000293": {
        "comment": [
            "SELEX begins with the synthesis of a large oligonucleotide library, either single-stranded DNA or RNA, which consists of random fixed-length sequences flanked by constant 5' and 3' ends that serve as primers. The library is exposed to a target ligand, typically a protein or small organic compound. Unbound sequences are removed, typically with affinity chromatography. Bound sequences are eluted and the nucleic acid is extracted, followed by PCR amplification (RNA is first reverse transcribed). PCR product is converted to single stranded (DNA) or transcribed (RNA). Oligonucleotides are bound to the ligand again and the process is repeated with increasing elution stringency. After several rounds of the process, recovered oligonucleotides are sequenced and analyzed to reveal the binding specificity of the protein."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-01-10T04:23:50Z"
        ],
        "def": [
            "\"A type of evidence arising from a physical interaction analysis where a combinatorial chemistry technique is used to identify oligonucleotides that bind to a target ligand.\" [ECO:MCC, url:http\\://en.wikipedia.org/wiki/Systematic_Evolution_of_Ligands_by_Exponential_Enrichment]"
        ],
        "id": [
            "ECO:0000293"
        ],
        "is_a": [
            "ECO:0000021 ! physical interaction evidence"
        ],
        "name": [
            "systematic evolution of ligands by exponential amplification evidence"
        ],
        "synonym": [
            "\"in vitro evolution evidence\" RELATED []",
            "\"in vitro selection evidence\" EXACT []",
            "\"SELEX evidence\" EXACT []"
        ],
        "xref": [
            "PSI-MI:MI\\:0657 \"systematic evolution of ligands by exponential enrichment\""
        ]
    },
    "ECO:0000294": {
        "comment": [
            "Bacterial one-hybrid assay is a method for screening a library of candidate proteins (prey plasmid) for the ability to bind to a target, cis-regulatory element or any other short, DNA binding sequence placed 5' to reporter genes (bait plasmid). Transformation of a bacterial host with two different plasmids is required: One is designed to express the \"bait\". The other plasmid contains a \"prey\" which, if bound to by the chimeric fusion product, drives expression of downstream reporter genes."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-01-10T06:16:20Z"
        ],
        "def": [
            "\"A type of hybrid interaction evidence that uses bacterial transformation with two plasmids to assess in vivo binding of a DNA-binding domain (bait) and DNA target site (prey).\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000294"
        ],
        "is_a": [
            "ECO:0000025 ! hybrid interaction evidence"
        ],
        "name": [
            "bacterial one-hybrid evidence"
        ],
        "synonym": [
            "\"B1H evidence\" EXACT []"
        ]
    },
    "ECO:0000295": {
        "comment": [
            "Total RNA is isolated from cells and mRNA is purified by targeting the 3' polyadenylated (poly(A)) tail with poly(T) oligos covalently attached to a substrate. Next, cDNA fragments are generated either by hydrolysis of RNA into 200-300 base oligonucleotides, preceded by reverse transcription, or by reverse transcription initiated by random primers. The cDNA second strand is synthesized, fragments are adapter ligated and high-throughput sequencing is performed by Roche 454, Illumina, ABI-SOLiD, or another HT sequencing technology. Resulting sort reads are aligned against a reference genome, and downstream analysis is performed."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-01-11T12:54:50Z"
        ],
        "def": [
            "\"A type of transcript expression evidence based on high-throughput (HT) sequencing of fragmented cDNA molecules.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000295"
        ],
        "is_a": [
            "ECO:0000009 ! transcript expression evidence"
        ],
        "name": [
            "RNA-seq evidence"
        ],
        "synonym": [
            "\"RNA sequencing\" RELATED []",
            "\"whole transcriptome shotgun sequencing\" EXACT [PMID:18611170]",
            "\"WTSS\" EXACT []"
        ]
    },
    "ECO:0000296": {
        "comment": [
            "This term describes transcriptional activation; to describe immunolocalization of the protein, use the term \"immunolocalization of green fluorescent protein\"."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-01-11T02:31:19Z"
        ],
        "def": [
            "\"A transcriptional activation assay that uses a green fluorescent protein (GFP) reporter gene.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000296"
        ],
        "is_a": [
            "ECO:0000170 ! transcriptional activation assay"
        ],
        "name": [
            "green fluorescent protein transcript localization evidence"
        ],
        "synonym": [
            "\"localization of GFP transcript\" EXACT []"
        ]
    },
    "ECO:0000297": {
        "comment": [
            "This term describes transcriptional activation; to describe immunolocalization of the protein, use the term \"immunolocalization of LacZ protein\"."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-01-11T02:53:19Z"
        ],
        "def": [
            "\"A transcriptional activation assay that uses a LacZ reporter gene.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000297"
        ],
        "is_a": [
            "ECO:0000170 ! transcriptional activation assay"
        ],
        "name": [
            "LacZ transcript localization evidence"
        ]
    },
    "ECO:0000298": {
        "comment": [
            "In the embryos of some organisms, inhibition of cytokinesis blocks neither the cell cycle nor the unfolding of the developmental program. As such, embryonic morphology can be frozen, allowing analysis of the developmental fate of each cell present at the time of cleavage arrest."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-01-11T03:28:49Z"
        ],
        "def": [
            "\"A type of experimental phenotypic evidence where embryonic development proceeds although cytokinesis is blocked.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000298"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "cleavage arrested development evidence"
        ]
    },
    "ECO:0000299": {
        "comment": [
            "Cytochalasin inhibits cytokinesis by blocking the formation of contractile microfilaments."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-01-11T03:30:29Z"
        ],
        "def": [
            "\"A type of cleavage arrested development that arises after treatment with cytochalasin.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000299"
        ],
        "is_a": [
            "ECO:0000298 ! cleavage arrested development evidence"
        ],
        "name": [
            "cytochalasin experiment evidence"
        ]
    },
    "ECO:0000300": {
        "comment": [
            "This term describes immunolocalization of the protein; to describe transcriptional activation, use the term \"localization of green fluorescent protein transcript\"."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-01-11T03:48:50Z"
        ],
        "def": [
            "\"A type of immunolocalization evidence data that was generated using green fluorescent protein (GFP) as a marker.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000300"
        ],
        "is_a": [
            "ECO:0000087 ! immunolocalization evidence"
        ],
        "name": [
            "green fluorescent protein immunolocalization evidence"
        ],
        "synonym": [
            "\"GFP immunolocalization evidence\" EXACT []"
        ]
    },
    "ECO:0000301": {
        "comment": [
            "This term describes immunolocalization of the protein; to describe transcriptional activation, use the term \"localization of LacZ transcript\"."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-01-11T04:06:40Z"
        ],
        "def": [
            "\"A type of immunolocalization evidence that was generated using LacZ protein as a marker.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000301"
        ],
        "is_a": [
            "ECO:0000087 ! immunolocalization evidence"
        ],
        "name": [
            "beta-galactosidase protein immunolocalization evidence"
        ],
        "synonym": [
            "\"LacZ protein immunolocalization evidence\" EXACT []"
        ]
    },
    "ECO:0000302": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-01-20T02:26:47Z"
        ],
        "def": [
            "\"A type of author statement that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000302"
        ],
        "intersection_of": [
            "ECO:0000204 ! author statement",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000204 ! author statement",
            "ECO:0000352 {is_inferred=\"true\"} ! evidence used in manual assertion"
        ],
        "name": [
            "author statement used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000303": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-01-20T02:29:50Z"
        ],
        "def": [
            "\"A type of non-traceable author statement that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000303"
        ],
        "intersection_of": [
            "ECO:0000034 ! non-traceable author statement",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000034 ! non-traceable author statement",
            "ECO:0000302 {is_inferred=\"true\"} ! author statement used in manual assertion"
        ],
        "name": [
            "non-traceable author statement used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ],
        "synonym": [
            "\"NAS\" EXACT [GOECO:NAS]",
            "\"non-traceable author statement\" EXACT [GOECO:NAS]"
        ],
        "xref": [
            "GOECO:NAS \"non-traceable author statement\""
        ]
    },
    "ECO:0000304": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-01-20T02:31:03Z"
        ],
        "def": [
            "\"A type of traceable author statement that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000304"
        ],
        "intersection_of": [
            "ECO:0000033 ! traceable author statement",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000033 ! traceable author statement",
            "ECO:0000302 {is_inferred=\"true\"} ! author statement used in manual assertion"
        ],
        "name": [
            "traceable author statement used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ],
        "synonym": [
            "\"TAS\" EXACT [GOECO:TAS]",
            "\"traceable author statement\" EXACT [GOECO:TAS]"
        ],
        "xref": [
            "GOECO:TAS \"traceable author statement\""
        ]
    },
    "ECO:0000305": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-01-20T02:33:32Z"
        ],
        "def": [
            "\"A type of curator inference that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000305"
        ],
        "intersection_of": [
            "ECO:0000205 ! curator inference",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000205 ! curator inference",
            "ECO:0000352 {is_inferred=\"true\"} ! evidence used in manual assertion"
        ],
        "name": [
            "curator inference used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ],
        "subset": [
            "valid_with_biological_process",
            "valid_with_cellular_component",
            "valid_with_molecular_function"
        ],
        "xref": [
            "GOECO:IC \"inferred by curator\""
        ]
    },
    "ECO:0000306": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-01-20T02:35:11Z"
        ],
        "def": [
            "\"A type of inference from background scientific knowledge that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000306"
        ],
        "intersection_of": [
            "ECO:0000001 ! inference from background scientific knowledge",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000001 ! inference from background scientific knowledge",
            "ECO:0000305 {is_inferred=\"true\"} ! curator inference used in manual assertion"
        ],
        "name": [
            "inference from background scientific knowledge used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000307": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-01-20T02:43:18Z"
        ],
        "def": [
            "\"A type of no biological data found that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000307"
        ],
        "intersection_of": [
            "ECO:0000035 ! no biological data found",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000035 ! no biological data found",
            "ECO:0000305 {is_inferred=\"true\"} ! curator inference used in manual assertion"
        ],
        "name": [
            "no biological data found used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ],
        "xref": [
            "GOECO:ND \"no biological data available\""
        ]
    },
    "ECO:0000308": {
        "comment": [
            "First, a characteristic of the most recent common ancestor (MRCA) is inferred from experimental evidence in descendants; this is followed by inferring a characteristic of a descendant of the MRCA. This type of evidence can be used in support of both positive and \"not\" GO annotations."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-02-04T05:41:26Z"
        ],
        "def": [
            "\"A type of phylogenetic evidence whereby an aspect of a descendant gene is inferred through the characterization of an aspect of an ancestral gene.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000308"
        ],
        "is_a": [
            "ECO:0000080 ! phylogenetic evidence"
        ],
        "name": [
            "biological aspect of ancestor evidence"
        ]
    },
    "ECO:0000309": {
        "comment": [
            "Complementary DNA (cDNA) is synthesized from microgram quantities of mRNA using first-strand cDNA primer (oligo dT12-18) and reverse transcriptase in the presence of trehalose and sorbitol, followed by selection of full-length cDNA with biotinylated cap-trapper. Linkers are attached to the 5' ends of full-length enriched cDNAs to introduce a recognition site for the restriction endonuclease MmeI, which creates a two base overhang. After amplification, sequencing tags are concatenated for high-throughput sequencing. Resulting sort reads are aligned against a reference genome, and downstream analysis is performed."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-02-17T01:33:09Z"
        ],
        "def": [
            "\"A type of transcript expression evidence based on high-throughput (HT) sequencing of the 5' ends of cDNA molecules that are selected by a cap-trapper system.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000309"
        ],
        "is_a": [
            "ECO:0000009 ! transcript expression evidence"
        ],
        "name": [
            "cap analysis of gene expression evidence"
        ],
        "synonym": [
            "\"CAGE\" EXACT [PMID:16489339]"
        ]
    },
    "ECO:0000310": {
        "comment": [
            "NanoCAGE captures the 5' ends of molecules by template switching. When polymerizing the cDNA of a capped mRNA, the reverse transcriptase adds extra cytosines that are complementary to the cap. Each 5' full length cDNAs is extended upon hybridization of the riboguanosine-tailed template switching oligonucleotides to these extra cytosines. In semisuppressive PCR, the short templates fold intramolecularly and prevent the binding of primers, which precludes amplification; longer molecules are less likely to fold and are thus amplified. Templates derived from reaction artifacts form stable homoduplexes, also precluding amplification. After template switching, semisuppressive PCR, and EcoP151 cleavage, 25-bp tags are ligated to bar code-containing oligonucleotide adaptors. After PCR amplification, the nanoCAGE tags are sequenced by synthesis. NanoCAGE requires as little as 10 nanograms of total RNA."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-02-17T01:33:34Z"
        ],
        "def": [
            "\"A type of transcript expression evidence based on high-throughput (HT) sequencing of the 5' ends of cDNA molecules that are selected by reverse transcriptase template switching.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000310"
        ],
        "is_a": [
            "ECO:0000009 ! transcript expression evidence"
        ],
        "name": [
            "nano-cap analysis of gene expression evidence"
        ],
        "synonym": [
            "\"nanoCAGE\" RELATED [PMID:20543846]"
        ]
    },
    "ECO:0000311": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-03-02T05:24:13Z"
        ],
        "def": [
            "\"An evidence type that is based on work performed by a person or group prior to a use by different person or group.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000311"
        ],
        "is_a": [
            "ECO:0000000 ! evidence"
        ],
        "name": [
            "imported information"
        ]
    },
    "ECO:0000312": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-03-02T05:29:44Z"
        ],
        "def": [
            "\"A type of imported information that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000312"
        ],
        "intersection_of": [
            "ECO:0000311 ! imported information",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000311 ! imported information",
            "ECO:0000352 {is_inferred=\"true\"} ! evidence used in manual assertion"
        ],
        "name": [
            "imported information used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000313": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-03-02T05:33:43Z"
        ],
        "def": [
            "\"A type of imported information that is used in an automatic assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000313"
        ],
        "intersection_of": [
            "ECO:0000311 ! imported information",
            "used_in ECO:0000203 ! automatic assertion"
        ],
        "is_a": [
            "ECO:0000311 ! imported information",
            "ECO:0000501 {is_inferred=\"true\"} ! evidence used in automatic assertion"
        ],
        "name": [
            "imported information used in automatic assertion"
        ],
        "relationship": [
            "used_in ECO:0000203 {is_inferred=\"true\"} ! automatic assertion"
        ]
    },
    "ECO:0000314": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-10-28T04:59:31Z"
        ],
        "def": [
            "\"A type of direct assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000314"
        ],
        "intersection_of": [
            "ECO:0000002 ! direct assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000002 ! direct assay evidence",
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion"
        ],
        "name": [
            "direct assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ],
        "subset": [
            "valid_with_chemical_entity"
        ],
        "synonym": [
            "\"IDA\" RELATED [GOECO:IDA]",
            "\"inferred from direct assay\" EXACT [GOECO:IDA]"
        ],
        "xref": [
            "GOECO:IDA \"inferred from direct assay\""
        ]
    },
    "ECO:0000315": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-10-28T05:12:49Z"
        ],
        "def": [
            "\"A type of mutant phenotype evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000315"
        ],
        "intersection_of": [
            "ECO:0000015 ! mutant phenotype evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000015 ! mutant phenotype evidence",
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion"
        ],
        "name": [
            "mutant phenotype evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ],
        "subset": [
            "valid_with_chemical_entity",
            "valid_with_gene",
            "valid_with_protein",
            "valid_with_protein_complex"
        ],
        "synonym": [
            "\"IMP\" RELATED [GOECO:IMP]",
            "\"inferred from mutant phenotype\" RELATED [GOECO:IMP]"
        ],
        "xref": [
            "GOECO:IMP \"inferred from mutant phenotype\""
        ]
    },
    "ECO:0000316": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-10-28T05:14:42Z"
        ],
        "def": [
            "\"A type of genetic interaction experiment evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000316"
        ],
        "intersection_of": [
            "ECO:0000011 ! genetic interaction evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000011 ! genetic interaction evidence",
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion"
        ],
        "name": [
            "genetic interaction evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ],
        "subset": [
            "valid_with_gene",
            "valid_with_protein",
            "valid_with_protein_complex"
        ],
        "synonym": [
            "\"IGI\" EXACT [GOECO:IGI]",
            "\"inferred from genetic interaction\" EXACT [GOECO:IGI]"
        ],
        "xref": [
            "GOECO:IGI \"inferred from genetic interaction\""
        ]
    },
    "ECO:0000317": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-10-28T05:22:53Z"
        ],
        "def": [
            "\"A type of genomic context evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000317"
        ],
        "intersection_of": [
            "ECO:0000177 ! genomic context evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000177 ! genomic context evidence",
            "ECO:0000352 {is_inferred=\"true\"} ! evidence used in manual assertion"
        ],
        "name": [
            "genomic context evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ],
        "subset": [
            "valid_with_gene",
            "valid_with_protein"
        ],
        "synonym": [
            "\"IGC\" RELATED [GOECO:IGC]",
            "\"inferred from genomic context\" EXACT [GOECO:IGC]"
        ],
        "xref": [
            "GOECO:IGC \"inferred from genomic context\""
        ]
    },
    "ECO:0000318": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-10-28T05:23:51Z"
        ],
        "def": [
            "\"A type of biological aspect of ancestor evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000318"
        ],
        "intersection_of": [
            "ECO:0000308 ! biological aspect of ancestor evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000252 {is_inferred=\"true\"} ! similarity evidence used in manual assertion",
            "ECO:0000308 ! biological aspect of ancestor evidence"
        ],
        "name": [
            "biological aspect of ancestor evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ],
        "subset": [
            "valid_with_gene",
            "valid_with_protein"
        ],
        "synonym": [
            "\"IBA\" EXACT [GOECO:IBA]",
            "\"inferred from biological aspect of ancestor\" EXACT [GOECO:IBA]"
        ],
        "xref": [
            "GOECO:IBA \"inferred from biological aspect of ancestor\""
        ]
    },
    "ECO:0000319": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-10-28T05:25:09Z"
        ],
        "def": [
            "\"A type of biological aspect of descendant evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000319"
        ],
        "intersection_of": [
            "ECO:0000214 ! biological aspect of descendant evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000214 ! biological aspect of descendant evidence",
            "ECO:0000252 {is_inferred=\"true\"} ! similarity evidence used in manual assertion"
        ],
        "name": [
            "biological aspect of descendant evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ],
        "subset": [
            "valid_with_gene",
            "valid_with_protein"
        ],
        "synonym": [
            "\"IBD\" EXACT [GOECO:IBD]",
            "\"inferred from biological aspect of descendant\" EXACT [GOECO:IBD]"
        ],
        "xref": [
            "GOECO:IBD \"inferred from biological aspect of descendant\""
        ]
    },
    "ECO:0000320": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-10-28T05:25:48Z"
        ],
        "def": [
            "\"A type of phylogenetic determination of loss of key residues evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000320"
        ],
        "intersection_of": [
            "ECO:0000216 ! phylogenetic determination of loss of key residues evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000216 ! phylogenetic determination of loss of key residues evidence",
            "ECO:0000252 {is_inferred=\"true\"} ! similarity evidence used in manual assertion"
        ],
        "name": [
            "phylogenetic determination of loss of key residues evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ],
        "synonym": [
            "\"IKR\" EXACT [GOECO:IKR]",
            "\"IMR\" EXACT [GOECO:IKR]",
            "\"inferred from key residues\" EXACT [GOECO:IKR]",
            "\"inferred from missing residues\" EXACT [GOECO:IKR]"
        ],
        "xref": [
            "GOECO:IKR \"inferred from key residues\"",
            "GOECO:IMR \"inferred from missing residues\""
        ]
    },
    "ECO:0000321": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-10-28T05:26:42Z"
        ],
        "def": [
            "\"A type of rapid divergence from ancestral sequence evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000321"
        ],
        "intersection_of": [
            "ECO:0000215 ! rapid divergence from ancestral sequence evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000215 ! rapid divergence from ancestral sequence evidence",
            "ECO:0000252 {is_inferred=\"true\"} ! similarity evidence used in manual assertion"
        ],
        "name": [
            "rapid divergence from ancestral sequence evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ],
        "subset": [
            "valid_with_gene",
            "valid_with_protein"
        ],
        "synonym": [
            "\"inferred from rapid divergence\" EXACT [GOECO:IRD]",
            "\"IRD\" EXACT [GOECO:IRD]"
        ],
        "xref": [
            "GOECO:IRD \"inferred from rapid divergence\""
        ]
    },
    "ECO:0000322": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-12-13T12:04:48Z"
        ],
        "def": [
            "\"Evidence that was initially used in manual assertion by one person or group, which is imported by a second person or group and used in automatic assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000322"
        ],
        "is_a": [
            "ECO:0000313 ! imported information used in automatic assertion"
        ],
        "name": [
            "imported manually asserted information used in automatic assertion"
        ],
        "xref": [
            "GO_REF:0000041 \"IEA (UniProt UniPathway)\""
        ]
    },
    "ECO:0000323": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2011-12-13T02:06:25Z"
        ],
        "def": [
            "\"Evidence that was initially used in automatic assertion by one person or group, which is imported by a second person or group and used in automatic assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000323"
        ],
        "is_a": [
            "ECO:0000313 ! imported information used in automatic assertion"
        ],
        "name": [
            "imported automatically asserted information used in automatic assertion"
        ]
    },
    "ECO:0000324": {
        "alt_id": [
            "ECO:0001122"
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2012-02-23T12:01:43Z"
        ],
        "def": [
            "\"Direct assay evidence derived from analysis of an image.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000324"
        ],
        "is_a": [
            "ECO:0000002 ! direct assay evidence"
        ],
        "name": [
            "imaging assay evidence"
        ],
        "synonym": [
            "\"radiologic test evidence\" NARROW []"
        ]
    },
    "ECO:0000325": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2012-05-16T11:46:27Z"
        ],
        "def": [
            "\"A type of experimental evidence that is based on separation of constituent parts of a mixture (the mobile phase) as they pass differentially through a stationary phase due to differences in partition coefficient and retention on the stationary phase.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000325"
        ],
        "is_a": [
            "ECO:0000006 ! experimental evidence"
        ],
        "name": [
            "chromatography evidence"
        ],
        "synonym": [
            "\"chromatographic evidence\" EXACT []"
        ]
    },
    "ECO:0000326": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2012-06-08T11:03:59Z"
        ],
        "def": [
            "\"A type of sequence alignment evidence that is based on comparing the position of exon junctions, relative to a reference genome, for a transcript annotation and a supporting transcript.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000326"
        ],
        "is_a": [
            "ECO:0000200 ! sequence alignment evidence"
        ],
        "name": [
            "transcript splice pattern evidence"
        ]
    },
    "ECO:0000327": {
        "comment": [
            "This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that have the same splice pattern as a RefSeq transcript. If such full transcript information is not available, RefSeq reports sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, supported by the evidence term ECO:0000328 or a child."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2012-06-08T11:11:24Z"
        ],
        "def": [
            "\"A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000327"
        ],
        "is_a": [
            "ECO:0000326 ! transcript splice pattern evidence"
        ],
        "name": [
            "whole transcript splice pattern evidence"
        ]
    },
    "ECO:0000328": {
        "comment": [
            "This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, but for which full transcript information is unavailable. For sequences that have the same splice pattern as a whole RefSeq transcript, use the evidence term ECO:0000327 or a child."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2012-06-08T11:21:57Z"
        ],
        "def": [
            "\"A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000328"
        ],
        "is_a": [
            "ECO:0000326 ! transcript splice pattern evidence"
        ],
        "name": [
            "coding sequence splice pattern evidence"
        ]
    },
    "ECO:0000329": {
        "comment": [
            "This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that have the same splice pattern as a RefSeq transcript. If such full transcript information is not available, RefSeq reports sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, supported by the evidence term ECO:0000328 or a child."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2012-06-08T11:40:03Z"
        ],
        "def": [
            "\"A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features, and is used in manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000329"
        ],
        "intersection_of": [
            "ECO:0000327 ! whole transcript splice pattern evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000247 {is_inferred=\"true\"} ! sequence alignment evidence used in manual assertion",
            "ECO:0000327 ! whole transcript splice pattern evidence"
        ],
        "name": [
            "whole transcript splice pattern evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000330": {
        "comment": [
            "This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, but for which full transcript information is unavailable. For sequences that have the same splice pattern as a whole RefSeq transcript, use the evidence term ECO:0000327 or a child."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2012-06-08T11:43:47Z"
        ],
        "def": [
            "\"A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript, and is used in manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000330"
        ],
        "intersection_of": [
            "ECO:0000328 ! coding sequence splice pattern evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000247 {is_inferred=\"true\"} ! sequence alignment evidence used in manual assertion",
            "ECO:0000328 ! coding sequence splice pattern evidence"
        ],
        "name": [
            "coding sequence splice pattern evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000331": {
        "comment": [
            "This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, but for which full transcript information is unavailable. For sequences that have the same splice pattern as a whole RefSeq transcript, use the evidence term ECO:0000327 or a child."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2012-06-08T11:48:09Z"
        ],
        "def": [
            "\"A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript, and is used in automatic assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000331"
        ],
        "intersection_of": [
            "ECO:0000328 ! coding sequence splice pattern evidence",
            "used_in ECO:0000203 ! automatic assertion"
        ],
        "is_a": [
            "ECO:0000248 {is_inferred=\"true\"} ! sequence alignment evidence used in automatic assertion",
            "ECO:0000328 ! coding sequence splice pattern evidence"
        ],
        "name": [
            "coding sequence splice pattern evidence used in automatic assertion"
        ],
        "relationship": [
            "used_in ECO:0000203 {is_inferred=\"true\"} ! automatic assertion"
        ]
    },
    "ECO:0000332": {
        "comment": [
            "This term is used by the NCBI Reference Sequences (RefSeq) in support of sequences that have the same splice pattern as a RefSeq transcript. If such full transcript information is not available, RefSeq reports sequences that match the splice pattern of a coding sequence (CDS) annotated on a RefSeq, supported by the evidence term ECO:0000328 or a child."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2012-06-08T11:49:17Z"
        ],
        "def": [
            "\"A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features, and is used in automatic assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000332"
        ],
        "intersection_of": [
            "ECO:0000327 ! whole transcript splice pattern evidence",
            "used_in ECO:0000203 ! automatic assertion"
        ],
        "is_a": [
            "ECO:0000248 {is_inferred=\"true\"} ! sequence alignment evidence used in automatic assertion",
            "ECO:0000327 ! whole transcript splice pattern evidence"
        ],
        "name": [
            "whole transcript splice pattern evidence used in automatic assertion"
        ],
        "relationship": [
            "used_in ECO:0000203 {is_inferred=\"true\"} ! automatic assertion"
        ]
    },
    "ECO:0000333": {
        "comment": [
            "Electrophoretic mobility is a function of both the length and charge of a protein. The protein sample is linearized with sodium dodecyl sulfate (SDS), an anionic detergent. Typically, polypeptides bound with SDS possess an even distribution of charge per unit mass, and subsequent fractionation is by size."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2012-07-31T02:56:43Z"
        ],
        "def": [
            "\"A type of gel electrophoresis evidence where proteins are separated according to their electrophoretic mobility.\" [ECO:MCC, PubMed:4861258, url:http\\://www.nature.com/nmeth/journal/v2/n1/full/nmeth0105-83.html, url:http\\://www.ncbi.nlm.nih.gov/Class/NAWBIS/Modules/Protein/protein18.html]"
        ],
        "id": [
            "ECO:0000333"
        ],
        "is_a": [
            "ECO:0000337 ! gel electrophoresis evidence"
        ],
        "name": [
            "sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence"
        ],
        "synonym": [
            "\"SDS-PAGE evidence\" EXACT []"
        ]
    },
    "ECO:0000334": {
        "comment": [
            "An example of an instrument used to analyze particle sizes and counts is the Beckman Coulter\u00c2\u00ae Z Series system."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2012-07-31T03:19:32Z"
        ],
        "def": [
            "\"A type of direct assay evidence resulting from an assay where both the size of and number of particles in a sample are determined.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000334"
        ],
        "is_a": [
            "ECO:0000002 ! direct assay evidence"
        ],
        "name": [
            "particle size and count assay evidence"
        ]
    },
    "ECO:0000335": {
        "comment": [
            "Two examples of substance quantification assay are (i) measuring how much of a radiolabelled compound is taken up by a cell and (ii) measuring how much of a substrate is turned over by a given enzyme."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2012-07-31T03:52:46Z"
        ],
        "def": [
            "\"A type of direct assay evidence where the quantity of a substance used as part of an assay is measured.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000335"
        ],
        "is_a": [
            "ECO:0000002 ! direct assay evidence"
        ],
        "name": [
            "substance quantification evidence"
        ]
    },
    "ECO:0000336": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2012-08-24T12:03:58Z"
        ],
        "def": [
            "\"A type of biological assay evidence in which a mutated strain of a microorganism, such as a yeast or bacterium, is grown competitively with wild-type cells and the relative fitness of the strains is assessed.\" [GOC:MAH, PMID:14718172, PMID:20537132]"
        ],
        "id": [
            "ECO:0000336"
        ],
        "is_a": [
            "ECO:0000094 ! biological assay evidence"
        ],
        "name": [
            "competitive growth assay evidence"
        ],
        "synonym": [
            "\"fitness profiling\" EXACT [GOC:MAH, PMID:20537132]"
        ]
    },
    "ECO:0000337": {
        "comment": [
            "Molecules typically separated include DNA, RNA, or protein. The gel is typically made of agarose, polyacrylamide, or starch."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2012-08-24T12:11:51Z"
        ],
        "def": [
            "\"A type of direct assay evidence where molecules have been sorted according to their size and charge by moving through a gel in the presence of an electric field.\" [ECO:MCC, wikipedia:Gel_electrophoresis]"
        ],
        "id": [
            "ECO:0000337"
        ],
        "is_a": [
            "ECO:0000002 ! direct assay evidence"
        ],
        "name": [
            "gel electrophoresis evidence"
        ]
    },
    "ECO:0000338": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2012-08-24T12:34:01Z"
        ],
        "def": [
            "\"Gel electrophoresis evidence that employs alternately pulsed, perpendicularly oriented electrical fields, at least one of which is inhomogeneous, to separate large DNA molecules on an agarose gel.\" [PMID:6373014]"
        ],
        "id": [
            "ECO:0000338"
        ],
        "is_a": [
            "ECO:0000337 ! gel electrophoresis evidence"
        ],
        "name": [
            "pulsed-field gel electrophoresis evidence"
        ],
        "synonym": [
            "\"PFGE\" EXACT []"
        ]
    },
    "ECO:0000339": {
        "comment": [
            "The first dimension gel is intentionally run at low voltage in low percentage agarose to separate DNA molecules in proportion to their mass. The second dimension is run at high voltage in a gel of higher agarose concentration in the presence of ethidium bromide so that the mobility of a non-linear molecule is drastically influenced by its shape."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2012-08-24T12:42:37Z"
        ],
        "def": [
            "\"A type of gel electrophoresis evidence where DNA molecules are electrophoresed on a low percentage agarose gel followed by high voltage electrophoresis on a higher percentage agarose gel in the presence of ethidium bromide.\" [PMID:16118435, PMID:8594382]"
        ],
        "id": [
            "ECO:0000339"
        ],
        "is_a": [
            "ECO:0000337 ! gel electrophoresis evidence"
        ],
        "name": [
            "two-dimensional agarose gel electrophoresis evidence"
        ],
        "synonym": [
            "\"2-D agarose gel electrophoresis\" EXACT []"
        ]
    },
    "ECO:0000340": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2012-08-24T01:27:58Z"
        ],
        "def": [
            "\"Experimental evidence from an assay in which a mitotically stable plasmid (or minichromosome) is introduced into cells, cells are grown without selection for several generations, the proportion of cells that retain the plasmid is then determined (by comparing colony numbers on selective versus non-selective media), and the loss per generation calculated.\" [ECO:MCC, PMID:6323245]"
        ],
        "id": [
            "ECO:0000340"
        ],
        "is_a": [
            "ECO:0000006 ! experimental evidence"
        ],
        "name": [
            "plasmid maintenance assay evidence"
        ],
        "synonym": [
            "\"minichromosome maintenance assay evidence\" EXACT [PMID:6323245]"
        ]
    },
    "ECO:0000341": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2012-08-24T01:48:24Z"
        ],
        "def": [
            "\"A type of specific protein inhibition evidence where the molecular function of a protein is inhibited by an antibody.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000341"
        ],
        "is_a": [
            "ECO:0000020 ! specific protein inhibition evidence"
        ],
        "name": [
            "specific protein inhibition by antibody evidence"
        ]
    },
    "ECO:0000342": {
        "comment": [
            "RNA-seq alignment intron location evidence is used by NCBI RefSeq (http://www.ncbi.nlm.nih.gov/refseq). Exon-exon boundaries (or intron locations) of a RefSeq transcript, as determined by aligning the transcript to a reference chromosome, are compared to intron locations supported by RNAseq data."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2013-04-03T04:22:05Z"
        ],
        "def": [
            "\"A type of transcript splice pattern evidence where intron locations in predicted transcripts are compared to intron locations supported by RNA-seq evidence.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000342"
        ],
        "is_a": [
            "ECO:0000295 ! RNA-seq evidence"
        ],
        "name": [
            "Support of intron position by RNA-seq alignment evidence"
        ]
    },
    "ECO:0000343": {
        "comment": [
            "Full support entails that all exon pairs represented in the transcript are supported. For example, for a transcript containing five exons, if liver RNA-seq reads support all four introns but brain RNA-seq supports only three introns, then the liver data fully support the transcript exons whereas the brain data do not."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2013-04-03T04:41:48Z"
        ],
        "def": [
            "\"A type of support of intron position by RNA-seq alignment evidence where RNA-seq alignment from a single sample supports all of the intron positions (exon pairs) predicted for a transcript.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000343"
        ],
        "is_a": [
            "ECO:0000342 ! Support of intron position by RNA-seq alignment evidence"
        ],
        "name": [
            "Full support of intron position by RNA-seq alignment evidence"
        ]
    },
    "ECO:0000344": {
        "comment": [
            "Partial support can occur in two ways. First, one or more exon-exon pair (intron) is not supported by any RNA-seq sample included in the analysis. Second, no single RNA-seq sample provides support for all exon-exon pairs (introns) represented in a transcript, but all represented exons are supported by at least one sample."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2013-04-03T04:50:46Z"
        ],
        "def": [
            "\"A type of support of intron position by RNA-seq alignment evidence where RNA-seq alignment to a genome supports only some of the intron positions (exon pairs) predicted for a transcript.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000344"
        ],
        "is_a": [
            "ECO:0000342 ! Support of intron position by RNA-seq alignment evidence"
        ],
        "name": [
            "Partial support of intron position by RNA-seq alignment evidence"
        ]
    },
    "ECO:0000345": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2013-04-03T05:10:34Z"
        ],
        "id": [
            "ECO:0000345"
        ],
        "is_a": [
            "ECO:0000200 ! sequence alignment evidence"
        ],
        "name": [
            "single exon transcript confirmation via alignment evidence"
        ]
    },
    "ECO:0000346": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2013-04-04T04:37:35Z"
        ],
        "def": [
            "\"A type of support of intron position by RNA-seq alignment evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000346"
        ],
        "intersection_of": [
            "ECO:0000342 ! Support of intron position by RNA-seq alignment evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000291 {is_inferred=\"true\"} ! transcript expression evidence used in manual assertion",
            "ECO:0000342 ! Support of intron position by RNA-seq alignment evidence"
        ],
        "name": [
            "Support of intron position by RNA-seq alignment evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000347": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2013-04-04T04:38:45Z"
        ],
        "def": [
            "\"A type of support of intron position by RNA-seq alignment evidence that is used in an automatic assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000347"
        ],
        "intersection_of": [
            "ECO:0000342 ! Support of intron position by RNA-seq alignment evidence",
            "used_in ECO:0000203 ! automatic assertion"
        ],
        "is_a": [
            "ECO:0000342 ! Support of intron position by RNA-seq alignment evidence",
            "ECO:0000501 {is_inferred=\"true\"} ! evidence used in automatic assertion"
        ],
        "name": [
            "Support of intron position by RNA-seq alignment evidence used in automatic assertion"
        ],
        "relationship": [
            "used_in ECO:0000203 {is_inferred=\"true\"} ! automatic assertion"
        ]
    },
    "ECO:0000348": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2013-04-04T04:40:19Z"
        ],
        "def": [
            "\"A type of full support of intron position by RNA-seq alignment evidence that is used in an automatic assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000348"
        ],
        "intersection_of": [
            "ECO:0000343 ! Full support of intron position by RNA-seq alignment evidence",
            "used_in ECO:0000203 ! automatic assertion"
        ],
        "is_a": [
            "ECO:0000343 ! Full support of intron position by RNA-seq alignment evidence",
            "ECO:0000347 {is_inferred=\"true\"} ! Support of intron position by RNA-seq alignment evidence used in automatic assertion"
        ],
        "name": [
            "Full support of intron position by RNA-seq alignment evidence used in automatic assertion"
        ],
        "relationship": [
            "used_in ECO:0000203 {is_inferred=\"true\"} ! automatic assertion"
        ]
    },
    "ECO:0000349": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2013-04-04T04:41:34Z"
        ],
        "def": [
            "\"A type of full support of intron position by RNA-seq alignment evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000349"
        ],
        "intersection_of": [
            "ECO:0000343 ! Full support of intron position by RNA-seq alignment evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000343 ! Full support of intron position by RNA-seq alignment evidence",
            "ECO:0000346 {is_inferred=\"true\"} ! Support of intron position by RNA-seq alignment evidence used in manual assertion"
        ],
        "name": [
            "Full support of intron position by RNA-seq alignment evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000350": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2013-04-04T04:42:53Z"
        ],
        "def": [
            "\"A type of partial support of intron position by RNA-seq alignment evidence that is used in an automatic assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000350"
        ],
        "intersection_of": [
            "ECO:0000344 ! Partial support of intron position by RNA-seq alignment evidence",
            "used_in ECO:0000203 ! automatic assertion"
        ],
        "is_a": [
            "ECO:0000344 ! Partial support of intron position by RNA-seq alignment evidence",
            "ECO:0000347 {is_inferred=\"true\"} ! Support of intron position by RNA-seq alignment evidence used in automatic assertion"
        ],
        "name": [
            "Partial support of intron position by RNA-seq alignment evidence used in automatic assertion"
        ],
        "relationship": [
            "used_in ECO:0000203 {is_inferred=\"true\"} ! automatic assertion"
        ]
    },
    "ECO:0000351": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2013-04-04T04:43:56Z"
        ],
        "def": [
            "\"A type of partial support of intron position by RNA-seq alignment evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000351"
        ],
        "intersection_of": [
            "ECO:0000344 ! Partial support of intron position by RNA-seq alignment evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000344 ! Partial support of intron position by RNA-seq alignment evidence",
            "ECO:0000346 {is_inferred=\"true\"} ! Support of intron position by RNA-seq alignment evidence used in manual assertion"
        ],
        "name": [
            "Partial support of intron position by RNA-seq alignment evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000352": {
        "created_by": [
            "mchibucos"
        ],
        "def": [
            "\"A type of evidence that is used in an manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000352"
        ],
        "intersection_of": [
            "ECO:0000000 ! evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000000 {is_inferred=\"true\"} ! evidence"
        ],
        "name": [
            "evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0000353": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2013-10-31T21:01:27Z"
        ],
        "def": [
            "\"A type of physical interaction evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000353"
        ],
        "intersection_of": [
            "ECO:0000021 ! physical interaction evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000021 ! physical interaction evidence",
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion"
        ],
        "name": [
            "physical interaction evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ],
        "subset": [
            "valid_with_chemical_entity",
            "valid_with_gene",
            "valid_with_protein",
            "valid_with_protein_complex"
        ],
        "synonym": [
            "\"inferred from physical interaction\" EXACT [GOECO:IPI]",
            "\"IPI\" EXACT [GOECO:IPI]"
        ],
        "xref": [
            "GOECO:IPI \"inferred from physical interaction\""
        ]
    },
    "ECO:0000354": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2013-11-19T13:37:24Z"
        ],
        "def": [
            "\"A type of gene neighbors evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000354"
        ],
        "intersection_of": [
            "ECO:0000084 ! gene neighbors evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000084 ! gene neighbors evidence",
            "ECO:0000317 ! genomic context evidence used in manual assertion"
        ],
        "name": [
            "gene neighbors evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ],
        "xref": [
            "GO_REF:0000025 \"operon structure as IGC evidence\""
        ]
    },
    "ECO:0000355": {
        "comment": [
            "Example of use: \"The presence of paired evaginated hemispheres and olfactory bulbs in both agnathan and gnathostome radiations suggests that such hemispheres were also present in the common ancestor.\" PMID: 7013637. Northcutt RG. (1981) Evolution of the telencephalon in nonmammals. Ann. Rev. Neurosci."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-04-22T17:02:31Z"
        ],
        "def": [
            "\"A type of phylogenetic evidence characterized by the mapping of the character states of living organisms onto phylogenies using the method of maximum parsimony.\" [ECO:MCC, PMID:21238344]"
        ],
        "id": [
            "ECO:0000355"
        ],
        "is_a": [
            "ECO:0000080 ! phylogenetic evidence"
        ],
        "name": [
            "phylogenetic distribution evidence"
        ]
    },
    "ECO:0000356": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-09-26T16:21:45Z"
        ],
        "id": [
            "ECO:0000356"
        ],
        "is_a": [
            "ECO:0000009 ! transcript expression evidence"
        ],
        "name": [
            "differential gene expression evidence from microarray experiment"
        ]
    },
    "ECO:0000357": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-09-26T16:22:57Z"
        ],
        "id": [
            "ECO:0000357"
        ],
        "is_a": [
            "ECO:0000009 ! transcript expression evidence"
        ],
        "name": [
            "differential gene expression evidence from RNA-seq experiment"
        ]
    },
    "ECO:0000358": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-09-26T16:25:47Z"
        ],
        "id": [
            "ECO:0000358"
        ],
        "is_a": [
            "ECO:0000009 ! transcript expression evidence"
        ],
        "name": [
            "differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact)"
        ]
    },
    "ECO:0000359": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-09-26T16:26:48Z"
        ],
        "id": [
            "ECO:0000359"
        ],
        "is_a": [
            "ECO:0000009 ! transcript expression evidence"
        ],
        "name": [
            "differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact)"
        ]
    },
    "ECO:0000360": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-09-26T16:39:11Z"
        ],
        "id": [
            "ECO:0000360"
        ],
        "is_a": [
            "ECO:0000006 ! experimental evidence"
        ],
        "name": [
            "biological target-disease association via drug"
        ]
    },
    "ECO:0000361": {
        "comment": [
            "Inference is the process of deriving logical conclusions from premises known or assumed to be true, or alternatively, arriving at conclusions via some non-logical means of observation of patterns of facts, which can result in identifying new meanings and contexts for understanding. Given the evidence provided by a set of premises, deductive reasoning involves drawing conclusions that necessarily follow, i.e. are certain, whereas inductive reasoning generates conclusions that are likely to be, but are not necessarily, true."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2015-07-14T11:19:37Z"
        ],
        "def": [
            "\"A type of evidence where an assertion is derived from another assertion via logical inference or some non-logical but rational means.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000361"
        ],
        "is_a": [
            "ECO:0000000 ! evidence"
        ],
        "name": [
            "inferential evidence"
        ]
    },
    "ECO:0000362": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2015-07-14T11:24:27Z"
        ],
        "def": [
            "\"A type of inferential evidence based on a computationally derived unary inference or an inference chain in which at least one step is \\ncomputationally derived.\" [ECO:MCC, GOC:MC]"
        ],
        "id": [
            "ECO:0000362"
        ],
        "is_a": [
            "ECO:0000361 ! inferential evidence"
        ],
        "name": [
            "evidence based on computational logical inference"
        ]
    },
    "ECO:0000363": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2015-07-14T11:35:08Z"
        ],
        "def": [
            "\"A type of evidence based on computational logical inference that is used in automatic assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000363"
        ],
        "intersection_of": [
            "ECO:0000362 ! evidence based on computational logical inference",
            "used_in ECO:0000203 ! automatic assertion"
        ],
        "is_a": [
            "ECO:0000362 ! evidence based on computational logical inference",
            "ECO:0000501 {is_inferred=\"true\"} ! evidence used in automatic assertion"
        ],
        "name": [
            "evidence based on computational logical inference used in automatic assertion"
        ],
        "relationship": [
            "used_in ECO:0000203 {is_inferred=\"true\"} ! automatic assertion"
        ],
        "xref": [
            "GO_REF:0000108 \"Automatic assignment of GO terms using logical inference, based on on inter-ontology links\""
        ]
    },
    "ECO:0000364": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2015-07-14T11:44:19Z"
        ],
        "def": [
            "\"A type of evidence based on logical inference from a manually curated annotation that is used in an automatic assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000364"
        ],
        "is_a": [
            "ECO:0000363 ! evidence based on computational logical inference used in automatic assertion"
        ],
        "name": [
            "evidence based on logical inference from manual annotation used in automatic assertion"
        ]
    },
    "ECO:0000366": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2015-07-14T11:45:25Z"
        ],
        "def": [
            "\"A type of evidence based on logical inference from an automatically curated annotation that is used in an automatic assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0000366"
        ],
        "is_a": [
            "ECO:0000363 ! evidence based on computational logical inference used in automatic assertion"
        ],
        "name": [
            "evidence based on logical inference from automatic annotation used in automatic assertion"
        ]
    },
    "ECO:0000501": {
        "created_by": [
            "cjm"
        ],
        "def": [
            "\"A type of evidence that is used in an automatic assertion.\" [ECO:cjm]"
        ],
        "id": [
            "ECO:0000501"
        ],
        "intersection_of": [
            "ECO:0000000 ! evidence",
            "used_in ECO:0000203 ! automatic assertion"
        ],
        "is_a": [
            "ECO:0000000 {is_inferred=\"true\"} ! evidence"
        ],
        "name": [
            "evidence used in automatic assertion"
        ],
        "relationship": [
            "used_in ECO:0000203 {is_inferred=\"true\"} ! automatic assertion"
        ],
        "subset": [
            "valid_with_gene",
            "valid_with_protein",
            "valid_with_protein_complex"
        ],
        "synonym": [
            "\"IEA\" EXACT [GOECO:IEA]",
            "\"inferred from electronic annotation\" EXACT [GOECO:IEA]"
        ],
        "xref": [
            "GO_REF:0000004 \"Gene Ontology annotation based on Swiss-Prot keyword mapping.\"",
            "GO_REF:0000023 \"Gene Ontology annotation based on Swiss-Prot Subcellular Location vocabulary mapping.\"",
            "GOECO:IEA \"inferred from electronic annotation\""
        ]
    },
    "ECO:0001001": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001001"
        ],
        "is_a": [
            "ECO:0005014 ! in vitro cell based assay evidence"
        ],
        "name": [
            "3D cell culture evidence"
        ]
    },
    "ECO:0001002": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001002"
        ],
        "is_a": [
            "ECO:0005014 ! in vitro cell based assay evidence"
        ],
        "name": [
            "[3H]arachidonic acid release assay evidence"
        ]
    },
    "ECO:0001003": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001003"
        ],
        "is_a": [
            "ECO:0005008 ! DNA synthesis cell proliferation assay evidence"
        ],
        "name": [
            "[3H]-thymidine incorporation assay evidence"
        ]
    },
    "ECO:0001004": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001004"
        ],
        "is_a": [
            "ECO:0005012 ! cytotoxicity assay evidence"
        ],
        "name": [
            "51Cr release assay evidence"
        ]
    },
    "ECO:0001005": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001005"
        ],
        "is_a": [
            "ECO:0005019 ! staining evidence"
        ],
        "name": [
            "7-aminoactinomycin staining evidence"
        ],
        "synonym": [
            "\"7-AAD staining evidence\" EXACT []",
            "\"7-amino-actinomycin D staining evidence\" EXACT []"
        ]
    },
    "ECO:0001006": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001006"
        ],
        "is_a": [
            "ECO:0005014 ! in vitro cell based assay evidence"
        ],
        "name": [
            "adhesion assay evidence"
        ]
    },
    "ECO:0001007": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of ex vivo assay evidence derived by ex vivo selection of tumor-reactive lymphocytes, and their activation and numerical expansion before re-infusion to the autologous tumor-bearing host.\" [ECO:SN, url:http\\://jco.ascopubs.org/content/23/10/2346.full.pdf+html, url:http\\://www.ncbi.nlm.nih.gov/pmc/articles/PMC2305722/pdf/nihms42807.pdf]"
        ],
        "id": [
            "ECO:0001007"
        ],
        "is_a": [
            "ECO:0005502 ! ex vivo assay evidence"
        ],
        "name": [
            "adoptive cell transfer evidence"
        ],
        "synonym": [
            "\"Adoptive immunotherapy\" EXACT []",
            "\"passive immunization\" EXACT []"
        ]
    },
    "ECO:0001008": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001008"
        ],
        "is_a": [
            "ECO:0005004 ! cell viability assay evidence"
        ],
        "name": [
            "Alamar blue assay evidence"
        ]
    },
    "ECO:0001009": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of anatomical perturbation evidence where an organ or tissue is transplanted from one individual to another of the same species with a different genotype.\" [ECO:SN, url:http\\://www.nature.com/subjects/allograft, url:http\\://www.organdonor.gov/about/terms_and_topics/]"
        ],
        "id": [
            "ECO:0001009"
        ],
        "is_a": [
            "ECO:0000240 ! anatomical perturbation evidence"
        ],
        "name": [
            "allograft transplantation experiment evidence"
        ],
        "synonym": [
            "\"allografting\" EXACT []",
            "\"Allotransplantation\" EXACT []",
            "\"tissue grafting\" NARROW []"
        ]
    },
    "ECO:0001010": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of chromatography evidence where a positively charged ion exchange resin with an affinity for molecules having net negative surface charges is used to separate the molecules.\" [ECO:SN, PMID:20978968, url:http\\://www.bio-rad.com/en-us/applications-technologies/anion-exchange-chromatography, url:http\\://www.uta.edu/faculty/sawasthi/Enzymology-4351-5324/Class%20Syllabus%20Enzymology/Ion%20Exchange%20Chromatography-1.pdf]"
        ],
        "id": [
            "ECO:0001010"
        ],
        "is_a": [
            "ECO:0000325 ! chromatography evidence"
        ],
        "name": [
            "anion-exchange chromatography evidence"
        ]
    },
    "ECO:0001011": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001011"
        ],
        "is_a": [
            "ECO:0005034 ! apoptotic assay evidence"
        ],
        "name": [
            "annexin-V staining evidence"
        ]
    },
    "ECO:0001012": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001012"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "behavioral assay evidence"
        ]
    },
    "ECO:0001013": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001013"
        ],
        "is_a": [
            "ECO:0000040 ! immunological assay evidence"
        ],
        "name": [
            "blocking monoclonal antibody evidence"
        ]
    },
    "ECO:0001014": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001014"
        ],
        "is_a": [
            "ECO:0000040 ! immunological assay evidence"
        ],
        "name": [
            "blocking peptide evidence"
        ]
    },
    "ECO:0001015": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001015"
        ],
        "is_a": [
            "ECO:0000040 ! immunological assay evidence"
        ],
        "name": [
            "blocking polyclonal antibody evidence"
        ]
    },
    "ECO:0001016": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of direct assay evidence where a blood sample is extracted from an organism to analyze different blood components.\" [ECO:SN, url:https\\://www.nhlbi.nih.gov/health/health-topics/topics/bdt]"
        ],
        "id": [
            "ECO:0001016"
        ],
        "is_a": [
            "ECO:0000002 ! direct assay evidence"
        ],
        "name": [
            "blood test evidence"
        ]
    },
    "ECO:0001017": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001017"
        ],
        "is_a": [
            "ECO:0005021 ! chemotaxis assay evidence"
        ],
        "name": [
            "Boyden chamber assay evidence"
        ]
    },
    "ECO:0001018": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001018"
        ],
        "is_a": [
            "ECO:0001107 ! nucleotide analog incorporation evidence"
        ],
        "name": [
            "bromodeoxyuridine incorporation assay evidence"
        ],
        "synonym": [
            "\"5-bromo-2'-deoxyuridine incorporation assay evidence\" EXACT []",
            "\"BrdU incorporation assay evidence\" EXACT []",
            "\"BrdUrd incorporation assay evidence\" EXACT []",
            "\"BUdR incorporation assay evidence\" EXACT []"
        ]
    },
    "ECO:0001019": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001019"
        ],
        "is_a": [
            "ECO:0005034 ! apoptotic assay evidence"
        ],
        "name": [
            "caspase assay evidence"
        ]
    },
    "ECO:0001020": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001020"
        ],
        "is_a": [
            "ECO:0005014 ! in vitro cell based assay evidence"
        ],
        "name": [
            "cell counting evidence"
        ]
    },
    "ECO:0001021": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001021"
        ],
        "is_a": [
            "ECO:0005014 ! in vitro cell based assay evidence"
        ],
        "name": [
            "cell permeability assay evidence"
        ]
    },
    "ECO:0001022": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001022"
        ],
        "is_a": [
            "ECO:0005019 ! staining evidence"
        ],
        "name": [
            "carboxyfluorescein diacetate succinimidyl ester staining evidence"
        ],
        "synonym": [
            "\"CFDA-SE staining evidence\" EXACT []",
            "\"CFSE-staining evidence\" EXACT []"
        ]
    },
    "ECO:0001023": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001023"
        ],
        "is_a": [
            "ECO:0005517 ! protein detection assay evidence"
        ],
        "name": [
            "chemiluminescence-linked immunoassay evidence"
        ],
        "synonym": [
            "\"CLIA evidence\" EXACT []"
        ]
    },
    "ECO:0001024": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001024"
        ],
        "is_a": [
            "ECO:0000015 ! mutant phenotype evidence"
        ],
        "name": [
            "chimeric protein evidence"
        ]
    },
    "ECO:0001025": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001025"
        ],
        "is_a": [
            "ECO:0000337 ! gel electrophoresis evidence"
        ],
        "name": [
            "co-electrophoresis evidence"
        ]
    },
    "ECO:0001026": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001026"
        ],
        "is_a": [
            "ECO:0000324 ! imaging assay evidence"
        ],
        "name": [
            "co-localization evidence"
        ]
    },
    "ECO:0001027": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001027"
        ],
        "is_a": [
            "ECO:0000002 ! direct assay evidence"
        ],
        "name": [
            "colony counting evidence"
        ]
    },
    "ECO:0001028": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001028"
        ],
        "is_a": [
            "ECO:0000021 ! physical interaction evidence"
        ],
        "name": [
            "co-sedimentation assay evidence"
        ]
    },
    "ECO:0001029": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001029"
        ],
        "is_a": [
            "ECO:0000337 ! gel electrophoresis evidence"
        ],
        "name": [
            "comet assay evidence"
        ]
    },
    "ECO:0001030": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001030"
        ],
        "is_a": [
            "ECO:0001091 ! knockout evidence"
        ],
        "name": [
            "conditional knockout evidence"
        ]
    },
    "ECO:0001031": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001031"
        ],
        "is_a": [
            "ECO:0001090 ! knockin evidence"
        ],
        "name": [
            "conditional knockin evidence"
        ]
    },
    "ECO:0001032": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001032"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "constitutively active mutant evidence"
        ]
    },
    "ECO:0001033": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001033"
        ],
        "is_a": [
            "ECO:0000024 ! protein binding evidence"
        ],
        "name": [
            "cross-linking evidence"
        ]
    },
    "ECO:0001034": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001034"
        ],
        "is_a": [
            "ECO:0005031 ! structure determination evidence"
        ],
        "name": [
            "crystallography evidence"
        ]
    },
    "ECO:0001035": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001035"
        ],
        "is_a": [
            "ECO:0000008 ! expression pattern evidence"
        ],
        "name": [
            "cytochemistry evidence"
        ]
    },
    "ECO:0001036": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001036"
        ],
        "is_a": [
            "ECO:0005034 ! apoptotic assay evidence"
        ],
        "name": [
            "cytochrome C release assay evidence"
        ]
    },
    "ECO:0001037": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001037"
        ],
        "is_a": [
            "ECO:0005019 ! staining evidence"
        ],
        "name": [
            "4',6-diamidino-2-phenylindole staining evidence"
        ],
        "synonym": [
            "\"DAPI staining evidence\" EXACT []"
        ]
    },
    "ECO:0001038": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001038"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "deletion evidence"
        ]
    },
    "ECO:0001039": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001039"
        ],
        "is_a": [
            "ECO:0005034 ! apoptotic assay evidence"
        ],
        "name": [
            "DNA laddering assay evidence"
        ]
    },
    "ECO:0001040": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of RNA detection assay evidence based on the direct application of a RNA mixture in a circular motion on a matrix for hybridization with labeled DNA fragments.\" [ECO:SW, PMID:21424648]"
        ],
        "id": [
            "ECO:0001040"
        ],
        "is_a": [
            "ECO:0005518 ! RNA detection assay evidence"
        ],
        "name": [
            "RNA dot blot assay evidence"
        ],
        "synonym": [
            "\"RNA dot blot\" RELATED []"
        ]
    },
    "ECO:0001041": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001041"
        ],
        "is_a": [
            "ECO:0005518 ! RNA detection assay evidence"
        ],
        "name": [
            "RNA microarray evidence"
        ]
    },
    "ECO:0001042": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001042"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "dominant-negative mutant evidence"
        ]
    },
    "ECO:0001043": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T16:59:00Z"
        ],
        "def": [
            "\"A type of Edman degradation evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001043"
        ],
        "intersection_of": [
            "ECO:0001044 ! Edman degradation evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001044 ! Edman degradation evidence"
        ],
        "name": [
            "Edman degradation evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001044": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001044"
        ],
        "is_a": [
            "ECO:0005517 ! protein detection assay evidence"
        ],
        "name": [
            "Edman degradation evidence"
        ]
    },
    "ECO:0001045": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001045"
        ],
        "is_a": [
            "ECO:0000023 ! affinity evidence"
        ],
        "name": [
            "eTag assay evidence"
        ]
    },
    "ECO:0001046": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001046"
        ],
        "is_a": [
            "ECO:0000021 ! physical interaction evidence"
        ],
        "name": [
            "filter binding assay evidence"
        ]
    },
    "ECO:0001047": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001047"
        ],
        "is_a": [
            "ECO:0000008 ! expression pattern evidence"
        ],
        "name": [
            "fluorescence in situ hybridization evidence"
        ]
    },
    "ECO:0001048": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001048"
        ],
        "is_a": [
            "ECO:0000021 ! physical interaction evidence"
        ],
        "name": [
            "fluorescence resonance energy transfer evidence"
        ],
        "synonym": [
            "\"FRET\" EXACT []"
        ]
    },
    "ECO:0001049": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001049"
        ],
        "is_a": [
            "ECO:0000325 ! chromatography evidence"
        ],
        "name": [
            "gel-filtration evidence"
        ]
    },
    "ECO:0001050": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001050"
        ],
        "is_a": [
            "ECO:0000008 ! expression pattern evidence"
        ],
        "name": [
            "histochemistry evidence"
        ]
    },
    "ECO:0001051": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001051"
        ],
        "is_a": [
            "ECO:0000324 ! imaging assay evidence"
        ],
        "name": [
            "histology evidence"
        ]
    },
    "ECO:0001052": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001052"
        ],
        "is_a": [
            "ECO:0000325 ! chromatography evidence"
        ],
        "name": [
            "high-performance liquid chromatography evidence"
        ],
        "synonym": [
            "\"HPLC evidence\" EXACT []"
        ]
    },
    "ECO:0001053": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001053"
        ],
        "is_a": [
            "ECO:0005517 ! protein detection assay evidence"
        ],
        "name": [
            "immunocytochemistry evidence"
        ]
    },
    "ECO:0001054": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001054"
        ],
        "is_a": [
            "ECO:0000040 ! immunological assay evidence"
        ],
        "name": [
            "immunodepletion evidence"
        ]
    },
    "ECO:0001055": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001055"
        ],
        "is_a": [
            "ECO:0005517 ! protein detection assay evidence"
        ],
        "name": [
            "immunohistochemistry evidence"
        ]
    },
    "ECO:0001056": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001056"
        ],
        "is_a": [
            "ECO:0005027 ! genetic transformation evidence"
        ],
        "name": [
            "induced mutation evidence"
        ]
    },
    "ECO:0001057": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vitro assay evidence where acetylated residues are detected in a compound (such as a protein or a nucleic acid) in an in vitro experiment.\" [ECO:SN, SIB:PG, url:http\\://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml]"
        ],
        "id": [
            "ECO:0001057"
        ],
        "is_a": [
            "ECO:0000181 ! in vitro assay evidence"
        ],
        "name": [
            "in vitro acetylation assay evidence"
        ]
    },
    "ECO:0001058": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vitro assay evidence where the cleavage of a protein by a protease is detected in an in vitro experiment. The cleavage gives rise to protein fragments.\" [ECO:SN, PMID:21121091, SIB:PG]"
        ],
        "id": [
            "ECO:0001058"
        ],
        "is_a": [
            "ECO:0000181 ! in vitro assay evidence"
        ],
        "name": [
            "in vitro cleavage assay evidence"
        ]
    },
    "ECO:0001059": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vitro assay evidence where the removal of ubiquitin groups on a protein is detected in vitro.\" [ECO:SN, PMID:19692941, SIB:PG]"
        ],
        "id": [
            "ECO:0001059"
        ],
        "is_a": [
            "ECO:0000181 ! in vitro assay evidence"
        ],
        "name": [
            "in vitro deubiquitination assay evidence"
        ]
    },
    "ECO:0001060": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vitro assay evidence where the removal of acetyl groups from a compound is detected in an in vitro experiment.\" [ECO:SN, SIB:PG, url:http\\://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml]"
        ],
        "id": [
            "ECO:0001060"
        ],
        "is_a": [
            "ECO:0000181 ! in vitro assay evidence"
        ],
        "name": [
            "in vitro deacetylation assay evidence"
        ]
    },
    "ECO:0001061": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vitro assay evidence where the removal of farnesyl groups from a protein is detected in an in vitro experiment.\" [ECO:SN, PMID:16126733, SIB:PG]"
        ],
        "id": [
            "ECO:0001061"
        ],
        "is_a": [
            "ECO:0000181 ! in vitro assay evidence"
        ],
        "name": [
            "in vitro defarnesylation assay evidence"
        ]
    },
    "ECO:0001062": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vitro assay evidence where the removal of methyl groups from a compound is detected in an in vitro experiment.\" [ECO:SN, SIB:PG, url:http\\://en.wikipedia.org/wiki/Demethylation]"
        ],
        "id": [
            "ECO:0001062"
        ],
        "is_a": [
            "ECO:0000181 ! in vitro assay evidence"
        ],
        "name": [
            "in vitro demethylation assay evidence"
        ]
    },
    "ECO:0001063": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vitro assay evidence where the removal of sumo groups is detected on a protein in vitro.\" [ECO:SN, SIB:PG, url:http\\://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/]"
        ],
        "id": [
            "ECO:0001063"
        ],
        "is_a": [
            "ECO:0000181 ! in vitro assay evidence"
        ],
        "name": [
            "in vitro desumoylation assay evidence"
        ]
    },
    "ECO:0001064": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vitro assay evidence where farnesylated residues in proteins are detected in an in vitro  experiment.\" [ECO:SN, SIB:PG, url:http\\://en.wikipedia.org/wiki/Farnesyltransferase]"
        ],
        "id": [
            "ECO:0001064"
        ],
        "is_a": [
            "ECO:0000181 ! in vitro assay evidence"
        ],
        "name": [
            "in vitro farnesylation assay evidence"
        ]
    },
    "ECO:0001065": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vitro assay evidence where methylated residues are detected in a compound (such as a protein or a nucleic acid) in an in vitro experiment.\" [ECO:SN, SIB:PG, url:http\\://en.wikipedia.org/wiki/Methylation]"
        ],
        "id": [
            "ECO:0001065"
        ],
        "is_a": [
            "ECO:0000181 ! in vitro assay evidence"
        ],
        "name": [
            "in vitro methylation assay evidence"
        ]
    },
    "ECO:0001066": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vitro assay evidence where palmitoylated residues in proteins are detected in an in vitro experiment.\" [ECO:SN, PMID:17077383, SIB:PG]"
        ],
        "id": [
            "ECO:0001066"
        ],
        "is_a": [
            "ECO:0000181 ! in vitro assay evidence"
        ],
        "name": [
            "in vitro palmitoylation assay evidence"
        ]
    },
    "ECO:0001067": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vitro assay evidence where the removal of phosphatase groups on a protein is detected in an in vitro experiment.\" [ECO:SN, SIB:PG, url:http\\://en.wikipedia.org/wiki/Phosphatase]"
        ],
        "id": [
            "ECO:0001067"
        ],
        "is_a": [
            "ECO:0000181 ! in vitro assay evidence"
        ],
        "name": [
            "in vitro phosphatase assay evidence"
        ]
    },
    "ECO:0001068": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vitro assay evidence where phosphorylated residues in a protein are detected in an in vitro experiment.\" [ECO:SN, SIB:PG, url:http\\://en.wikipedia.org/wiki/Protein_kinase, url:http\\://en.wikipedia.org/wiki/Protein_phosphorylation]"
        ],
        "id": [
            "ECO:0001068"
        ],
        "is_a": [
            "ECO:0000181 ! in vitro assay evidence"
        ],
        "name": [
            "in vitro protein kinase assay evidence"
        ]
    },
    "ECO:0001069": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vitro assay evidence where ADP-ribosylated residues in proteins are detected in an in vitro experiment.\" [ECO:SN, PMID:21870253, SIB:PG]"
        ],
        "id": [
            "ECO:0001069"
        ],
        "is_a": [
            "ECO:0000181 ! in vitro assay evidence"
        ],
        "name": [
            "in vitro polyADP-ribosylation assay evidence"
        ]
    },
    "ECO:0001070": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vitro assay evidence where sumoylated residues are detected on a protein in an in vitro experiment.\" [ECO:SN, SIB:PG, url:http\\://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/]"
        ],
        "id": [
            "ECO:0001070"
        ],
        "is_a": [
            "ECO:0000181 ! in vitro assay evidence"
        ],
        "name": [
            "in vitro sumoylation assay evidence"
        ]
    },
    "ECO:0001071": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vitro assay evidence where transcription is detected in vitro to validate the activation or repression ablity of a transcription factor in the absence of other proteins present in in vivo conditions.\" [ECO:SN, ECO:SW, PMID:21125481, SIB:PG]"
        ],
        "id": [
            "ECO:0001071"
        ],
        "is_a": [
            "ECO:0000181 ! in vitro assay evidence"
        ],
        "name": [
            "in vitro transcription assay evidence"
        ]
    },
    "ECO:0001072": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vitro assay evidence where de novo protein synthesis is detected in an in vitro experiment.\" [ECO:SN, PMID:18230759, SIB:PG]"
        ],
        "id": [
            "ECO:0001072"
        ],
        "is_a": [
            "ECO:0000181 ! in vitro assay evidence"
        ],
        "name": [
            "in vitro translation assay evidence"
        ]
    },
    "ECO:0001073": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vitro assay evidence where ubiquitinated residues are detected on a protein in an in vitro experiment.\" [ECO:SN, PMID:19692941, SIB:PG]"
        ],
        "id": [
            "ECO:0001073"
        ],
        "is_a": [
            "ECO:0000181 ! in vitro assay evidence"
        ],
        "name": [
            "in vitro ubiquitination assay evidence"
        ]
    },
    "ECO:0001074": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vivo assay evidence where acetylated residues are detected in a compound (such as a protein or a nucleic acid) in cells in vivo.\" [ECO:SN, SIB:PG, url:http\\://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml]"
        ],
        "id": [
            "ECO:0001074"
        ],
        "is_a": [
            "ECO:0000178 ! in vivo assay evidence"
        ],
        "name": [
            "in vivo acetylation assay evidence"
        ]
    },
    "ECO:0001075": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vivo assay evidence where the cleavage of a protein by a protease is detected in cells in vivo. The cleavage gives rise to protein fragments.\" [ECO:SN, PMID:22154596, SIB:PG]"
        ],
        "id": [
            "ECO:0001075"
        ],
        "is_a": [
            "ECO:0000178 ! in vivo assay evidence"
        ],
        "name": [
            "in vivo cleavage assay evidence"
        ]
    },
    "ECO:0001076": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vivo assay evidence where the removal of acetyl groups from a compound is detected in cells in vivo.\" [ECO:SN, SIB:PG, url:http\\://www.perkinelmer.com/resources/technicalresources/applicationsupportknowledgebase/radiometric/acetylation.xhtml]"
        ],
        "id": [
            "ECO:0001076"
        ],
        "is_a": [
            "ECO:0000178 ! in vivo assay evidence"
        ],
        "name": [
            "in vivo deacetylation assay evidence"
        ]
    },
    "ECO:0001077": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vivo assay evidence where removal of farnesyl groups from a protein is detected in cells in vivo.\" [ECO:SN, PMID:15556768, PMID:16507103, SIB:PG]"
        ],
        "id": [
            "ECO:0001077"
        ],
        "is_a": [
            "ECO:0000178 ! in vivo assay evidence"
        ],
        "name": [
            "in vivo defarnesylation assay evidence"
        ]
    },
    "ECO:0001078": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vivo assay evidence where the removal of methyl groups from a compound is detected in cells in vivo.\" [ECO:SN, SIB:PG, url:http\\://en.wikipedia.org/wiki/Demethylation]"
        ],
        "id": [
            "ECO:0001078"
        ],
        "is_a": [
            "ECO:0000178 ! in vivo assay evidence"
        ],
        "name": [
            "in vivo demethylation assay evidence"
        ]
    },
    "ECO:0001079": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vivo assay evidence where the removal of ubiquitin groups on a protein is detected in cells in vivo.\" [ECO:SN, PMID:19692941, SIB:PG]"
        ],
        "id": [
            "ECO:0001079"
        ],
        "is_a": [
            "ECO:0000178 ! in vivo assay evidence"
        ],
        "name": [
            "in vivo deubiquitination assay evidence"
        ]
    },
    "ECO:0001080": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vivo assay evidence where the removal of sumo groups on a protein is detected in cells in vivo.\" [ECO:SN, SIB:PG, url:http\\://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/]"
        ],
        "id": [
            "ECO:0001080"
        ],
        "is_a": [
            "ECO:0000178 ! in vivo assay evidence"
        ],
        "name": [
            "in vivo desumoylation assay evidence"
        ]
    },
    "ECO:0001081": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vivo assay evidence where farnesylated residues in proteins are detected in cells in vivo.\" [ECO:SN, PMID:9030603, SIB:PG]"
        ],
        "id": [
            "ECO:0001081"
        ],
        "is_a": [
            "ECO:0000178 ! in vivo assay evidence"
        ],
        "name": [
            "in vivo farnesylation assay evidence"
        ]
    },
    "ECO:0001082": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vivo assay evidence where methylated residues are detected in a compound (such as a protein or a nucleic acid) in vivo.\" [ECO:SN, SIB:PG, url:http\\://en.wikipedia.org/wiki/Methylation]"
        ],
        "id": [
            "ECO:0001082"
        ],
        "is_a": [
            "ECO:0000178 ! in vivo assay evidence"
        ],
        "name": [
            "in vivo methylation assay evidence"
        ]
    },
    "ECO:0001083": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vivo assay evidence where palmitoylated residues in proteins are detected in cells in vivo.\" [ECO:SN, PMID:10329400, SIB:PG]"
        ],
        "id": [
            "ECO:0001083"
        ],
        "is_a": [
            "ECO:0000178 ! in vivo assay evidence"
        ],
        "name": [
            "in vivo palmitoylation assay evidence"
        ]
    },
    "ECO:0001084": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vivo assay evidence where the removal of phosphate groups on a protein is detected in cells in vivo.\" [ECO:SN, SIB:PG, url:http\\://en.wikipedia.org/wiki/Phosphatase]"
        ],
        "id": [
            "ECO:0001084"
        ],
        "is_a": [
            "ECO:0000178 ! in vivo assay evidence"
        ],
        "name": [
            "in vivo phosphatase assay evidence"
        ]
    },
    "ECO:0001085": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vivo assay evidence where phosphorylated residues in a protein are detected in cells in vivo.\" [ECO:SN, SIB:PG, url:http\\://en.wikipedia.org/wiki/Protein_kinase, url:http\\://en.wikipedia.org/wiki/Protein_phosphorylation]"
        ],
        "id": [
            "ECO:0001085"
        ],
        "is_a": [
            "ECO:0000178 ! in vivo assay evidence"
        ],
        "name": [
            "in vivo protein kinase assay evidence"
        ]
    },
    "ECO:0001086": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vivo assay evidence where sumoylated residues are detected on a protein in cells in vivo.\" [ECO:SN, SIB:PG, url:http\\://www.enzolifesciences.com/BML-UW8955/sumoylation-kit/]"
        ],
        "id": [
            "ECO:0001086"
        ],
        "is_a": [
            "ECO:0000178 ! in vivo assay evidence"
        ],
        "name": [
            "in vivo sumoylation assay evidence"
        ]
    },
    "ECO:0001087": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vivo assay evidence where transcription is detected in cells in vivo.\" [ECO:SN, PMID:12181418, SIB:PG]"
        ],
        "id": [
            "ECO:0001087"
        ],
        "is_a": [
            "ECO:0000178 ! in vivo assay evidence"
        ],
        "name": [
            "in vivo transcription assay evidence"
        ]
    },
    "ECO:0001088": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vivo assay evidence where de novo protein synthesis is detected in cells in vivo.\" [ECO:SN, PMID:24901308, SIB:PG]"
        ],
        "id": [
            "ECO:0001088"
        ],
        "is_a": [
            "ECO:0000178 ! in vivo assay evidence"
        ],
        "name": [
            "in vivo translation assay evidence"
        ]
    },
    "ECO:0001089": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of in vivo assay evidence where ubiquitinated residues on a protein are detected in cells in vivo.\" [ECO:SN, PMID:19692941, SIB:PG]"
        ],
        "id": [
            "ECO:0001089"
        ],
        "is_a": [
            "ECO:0000178 ! in vivo assay evidence"
        ],
        "name": [
            "in vivo ubiquitination assay evidence"
        ]
    },
    "ECO:0001090": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001090"
        ],
        "is_a": [
            "ECO:0005027 ! genetic transformation evidence"
        ],
        "name": [
            "knockin evidence"
        ]
    },
    "ECO:0001091": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001091"
        ],
        "is_a": [
            "ECO:0005027 ! genetic transformation evidence"
        ],
        "name": [
            "knockout evidence"
        ]
    },
    "ECO:0001092": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001092"
        ],
        "is_a": [
            "ECO:0000021 ! physical interaction evidence"
        ],
        "name": [
            "lipid binding assay evidence"
        ]
    },
    "ECO:0001093": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001093"
        ],
        "is_a": [
            "ECO:0000021 ! physical interaction evidence"
        ],
        "name": [
            "luminescence-based mammalian interactome mapping assay evidence"
        ],
        "synonym": [
            "\"LUMIER assay\" EXACT []"
        ]
    },
    "ECO:0001094": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001094"
        ],
        "is_a": [
            "ECO:0000006 ! experimental evidence"
        ],
        "name": [
            "macroscopy evidence"
        ]
    },
    "ECO:0001095": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001095"
        ],
        "is_a": [
            "ECO:0000025 ! hybrid interaction evidence"
        ],
        "name": [
            "mammalian 2-hybrid assay evidence"
        ]
    },
    "ECO:0001096": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001096"
        ],
        "is_a": [
            "ECO:0005504 ! spectrometry evidence"
        ],
        "name": [
            "mass spectrometry evidence"
        ]
    },
    "ECO:0001097": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001097"
        ],
        "is_a": [
            "ECO:0000324 ! imaging assay evidence"
        ],
        "name": [
            "medical imaging evidence"
        ]
    },
    "ECO:0001098": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of direct assay evidence where a microscope was used to view a sample.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001098"
        ],
        "is_a": [
            "ECO:0000324 ! imaging assay evidence"
        ],
        "name": [
            "microscopy evidence"
        ]
    },
    "ECO:0001099": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001099"
        ],
        "is_a": [
            "ECO:0000181 ! in vitro assay evidence"
        ],
        "name": [
            "motility wound healing assay evidence"
        ]
    },
    "ECO:0001100": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001100"
        ],
        "is_a": [
            "ECO:0005034 ! apoptotic assay evidence"
        ],
        "name": [
            "MTS assay evidence"
        ],
        "synonym": [
            "\"3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium\" NARROW []"
        ]
    },
    "ECO:0001101": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001101"
        ],
        "is_a": [
            "ECO:0005034 ! apoptotic assay evidence"
        ],
        "name": [
            "MTT assay evidence"
        ],
        "synonym": [
            "\"3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide\" NARROW []"
        ]
    },
    "ECO:0001102": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001102"
        ],
        "is_a": [
            "ECO:0005517 ! protein detection assay evidence"
        ],
        "name": [
            "multiplex bead-based immunoassay evidence"
        ]
    },
    "ECO:0001103": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001103"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "natural variation mutant evidence"
        ]
    },
    "ECO:0001104": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001104"
        ],
        "is_a": [
            "ECO:0005034 ! apoptotic assay evidence"
        ],
        "name": [
            "nuclear fragmentation evidence"
        ]
    },
    "ECO:0001105": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001105"
        ],
        "is_a": [
            "ECO:0005031 ! structure determination evidence"
        ],
        "name": [
            "nuclear magnetic resonance spectroscopy evidence"
        ],
        "synonym": [
            "\"NMR spectroscopy\" EXACT []"
        ]
    },
    "ECO:0001106": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001106"
        ],
        "is_a": [
            "ECO:0005518 ! RNA detection assay evidence"
        ],
        "name": [
            "nuclease protection assay evidence"
        ]
    },
    "ECO:0001107": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001107"
        ],
        "is_a": [
            "ECO:0005008 ! DNA synthesis cell proliferation assay evidence"
        ],
        "name": [
            "nucleotide analog incorporation evidence"
        ]
    },
    "ECO:0001108": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001108"
        ],
        "is_a": [
            "ECO:0000024 ! protein binding evidence"
        ],
        "name": [
            "phage display evidence"
        ]
    },
    "ECO:0001109": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001109"
        ],
        "is_a": [
            "ECO:0000039 ! protein assay evidence"
        ],
        "name": [
            "phosphoamino acid analysis evidence"
        ]
    },
    "ECO:0001110": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001110"
        ],
        "is_a": [
            "ECO:0000023 ! affinity evidence"
        ],
        "name": [
            "peptide affinity enrichment evidence"
        ],
        "synonym": [
            "\"phosphopeptide affinity enrichment evidence\" NARROW []"
        ]
    },
    "ECO:0001111": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001111"
        ],
        "is_a": [
            "ECO:0000002 ! direct assay evidence"
        ],
        "name": [
            "physical examination evidence"
        ]
    },
    "ECO:0001112": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001112"
        ],
        "is_a": [
            "ECO:0000039 ! protein assay evidence"
        ],
        "name": [
            "peptide array evidence"
        ],
        "synonym": [
            "\"phosphopeptide array evidence\" NARROW []"
        ]
    },
    "ECO:0001113": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001113"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "point mutation evidence"
        ]
    },
    "ECO:0001114": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001114"
        ],
        "is_a": [
            "ECO:0005019 ! staining evidence"
        ],
        "name": [
            "propidium iodide staining evidence"
        ]
    },
    "ECO:0001115": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001115"
        ],
        "is_a": [
            "ECO:0000002 ! direct assay evidence"
        ],
        "name": [
            "fluorescence evidence"
        ]
    },
    "ECO:0001116": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001116"
        ],
        "is_a": [
            "ECO:0005517 ! protein detection assay evidence"
        ],
        "name": [
            "protein dot blot assay evidence"
        ]
    },
    "ECO:0001117": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001117"
        ],
        "is_a": [
            "ECO:0005517 ! protein detection assay evidence"
        ],
        "name": [
            "protein microarray evidence"
        ]
    },
    "ECO:0001118": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001118"
        ],
        "is_a": [
            "ECO:0000220 ! sequencing assay evidence"
        ],
        "name": [
            "protein sequencing assay evidence"
        ]
    },
    "ECO:0001119": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001119"
        ],
        "is_a": [
            "ECO:0001096 ! mass spectrometry evidence"
        ],
        "name": [
            "quantitative mass spectrometry evidence"
        ]
    },
    "ECO:0001120": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001120"
        ],
        "is_a": [
            "ECO:0000002 ! direct assay evidence"
        ],
        "name": [
            "radioisotope assay evidence"
        ],
        "synonym": [
            "\"radioassay\" EXACT []",
            "\"radioisotopic assay\" EXACT []"
        ]
    },
    "ECO:0001121": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001121"
        ],
        "is_a": [
            "ECO:0005517 ! protein detection assay evidence"
        ],
        "name": [
            "radioimmunoassay evidence"
        ]
    },
    "ECO:0001123": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001123"
        ],
        "is_a": [
            "ECO:0000002 ! direct assay evidence"
        ],
        "name": [
            "resonant mirror biosensor evidence"
        ]
    },
    "ECO:0001124": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001124"
        ],
        "is_a": [
            "ECO:0005519 ! DNA detection assay evidence"
        ],
        "name": [
            "restriction fragment detection evidence"
        ]
    },
    "ECO:0001126": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001126"
        ],
        "is_a": [
            "ECO:0005504 ! spectrometry evidence"
        ],
        "name": [
            "spectrophotometry evidence"
        ]
    },
    "ECO:0001127": {
        "comment": [
            "Plasmons are generated by an incident light beam."
        ],
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of physical interaction evidence based on real-time, rapid plasmon generation on the interface between a planar surface and vacuum that measures changes in refractive index close to the sensor surface when an analyte and its immobilized ligand bind to observe and characterize molecular interaction.\" [ECO:SW, PMID:11578932, PMID:19151937, PMID:8574707]"
        ],
        "id": [
            "ECO:0001127"
        ],
        "is_a": [
            "ECO:0000021 ! physical interaction evidence"
        ],
        "name": [
            "surface plasmon resonance evidence"
        ],
        "synonym": [
            "\"SPR evidence\" EXACT [PMID:11578932]"
        ]
    },
    "ECO:0001128": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001128"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "syngeneic transplantation experiment evidence"
        ]
    },
    "ECO:0001129": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001129"
        ],
        "is_a": [
            "ECO:0000005 ! enzyme assay evidence"
        ],
        "name": [
            "TACE activity assay evidence"
        ],
        "synonym": [
            "\"Tumor necrosis factor - alpha converting enzyme activity assay\" EXACT []"
        ]
    },
    "ECO:0001130": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001130"
        ],
        "is_a": [
            "ECO:0000055 ! array experiment evidence"
        ],
        "name": [
            "tissue microarray evidence"
        ]
    },
    "ECO:0001131": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001131"
        ],
        "is_a": [
            "ECO:0005027 ! genetic transformation evidence"
        ],
        "name": [
            "transgenic organism evidence"
        ]
    },
    "ECO:0001132": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001132"
        ],
        "is_a": [
            "ECO:0001096 ! mass spectrometry evidence"
        ],
        "name": [
            "tryptic phosphopeptide mapping assay evidence"
        ]
    },
    "ECO:0001133": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001133"
        ],
        "is_a": [
            "ECO:0005034 ! apoptotic assay evidence"
        ],
        "name": [
            "terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence"
        ],
        "synonym": [
            "\"TdT-mediated dUTP-biotin nick end labeling assay\" EXACT []",
            "\"terminal deoxynucleotidyl transferase-dUTP nick end labeling assay evidence\" EXACT []",
            "\"TUNEL assay evidence\" EXACT []"
        ]
    },
    "ECO:0001134": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001134"
        ],
        "is_a": [
            "ECO:0000002 ! direct assay evidence"
        ],
        "name": [
            "urine test evidence"
        ]
    },
    "ECO:0001135": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001135"
        ],
        "is_a": [
            "ECO:0005014 ! in vitro cell based assay evidence"
        ],
        "name": [
            "wound healing assay evidence"
        ]
    },
    "ECO:0001136": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "id": [
            "ECO:0001136"
        ],
        "is_a": [
            "ECO:0005007 ! cell proliferation assay evidence"
        ],
        "name": [
            "WST-1 assay evidence"
        ]
    },
    "ECO:0001137": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T11:30:54Z"
        ],
        "def": [
            "\"A type of anatomical perturbation evidence where live cells, tissues, or organs from a non-human animal source are transplanted, implanted or infused into a human recipient.\" [ECO:SN, url:http\\://ilarjournal.oxfordjournals.org/content/37/1/16.full.pdf+html, url:http\\://www.fda.gov/BiologicsBloodVaccines/Xenotransplantation/]"
        ],
        "id": [
            "ECO:0001137"
        ],
        "is_a": [
            "ECO:0000240 ! anatomical perturbation evidence"
        ],
        "name": [
            "xenotransplantation experiment evidence"
        ],
        "synonym": [
            "\"tissue grafting\" NARROW []",
            "\"Xenograft transplantation\" EXACT []",
            "\"Xenografting\" EXACT []",
            "\"Xenotransplantation\" EXACT []"
        ]
    },
    "ECO:0001138": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T15:18:35Z"
        ],
        "def": [
            "\"A type of 3D cell culture evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001138"
        ],
        "intersection_of": [
            "ECO:0001001 ! 3D cell culture evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001001 ! 3D cell culture evidence"
        ],
        "name": [
            "3D cell culture evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001139": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T15:20:55Z"
        ],
        "def": [
            "\"A type of 51Cr release assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001139"
        ],
        "intersection_of": [
            "ECO:0001004 ! 51Cr release assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001004 ! 51Cr release assay evidence"
        ],
        "name": [
            "51Cr release assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001140": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T15:22:01Z"
        ],
        "def": [
            "\"A type of 7-aminoactinomycin staining evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001140"
        ],
        "intersection_of": [
            "ECO:0001005 ! 7-aminoactinomycin staining evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001005 ! 7-aminoactinomycin staining evidence"
        ],
        "name": [
            "7-aminoactinomycin staining evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001141": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T15:31:52Z"
        ],
        "def": [
            "\"A type of [3H]-thymidine incorporation assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001141"
        ],
        "intersection_of": [
            "ECO:0001003 ! [3H]-thymidine incorporation assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001003 ! [3H]-thymidine incorporation assay evidence"
        ],
        "name": [
            "[3H]-thymidine incorporation assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001142": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T15:32:44Z"
        ],
        "def": [
            "\"A type of [3H]arachidonic acid release assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001142"
        ],
        "intersection_of": [
            "ECO:0001002 ! [3H]arachidonic acid release assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001002 ! [3H]arachidonic acid release assay evidence"
        ],
        "name": [
            "[3H]arachidonic acid release assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001143": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T15:33:38Z"
        ],
        "def": [
            "\"A type of adhesion assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001143"
        ],
        "intersection_of": [
            "ECO:0001006 ! adhesion assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001006 ! adhesion assay evidence"
        ],
        "name": [
            "adhesion assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001144": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T15:36:19Z"
        ],
        "def": [
            "\"A type of adoptive cell transfer evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001144"
        ],
        "intersection_of": [
            "ECO:0001007 ! adoptive cell transfer evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001007 ! adoptive cell transfer evidence"
        ],
        "name": [
            "adoptive cell transfer evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001145": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T15:37:21Z"
        ],
        "def": [
            "\"A type of Alamar blue assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001145"
        ],
        "intersection_of": [
            "ECO:0001008 ! Alamar blue assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001008 ! Alamar blue assay evidence"
        ],
        "name": [
            "Alamar blue assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001146": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T15:38:22Z"
        ],
        "def": [
            "\"A type of allograft transplantation experiment evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001146"
        ],
        "intersection_of": [
            "ECO:0001009 ! allograft transplantation experiment evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001009 ! allograft transplantation experiment evidence"
        ],
        "name": [
            "allograft transplantation experiment evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001147": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T15:44:30Z"
        ],
        "def": [
            "\"A type of anion-exchange chromatography evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001147"
        ],
        "intersection_of": [
            "ECO:0001010 ! anion-exchange chromatography evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001010 ! anion-exchange chromatography evidence"
        ],
        "name": [
            "anion-exchange chromatography evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001148": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T15:52:01Z"
        ],
        "def": [
            "\"A type of annexin-V staining evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001148"
        ],
        "intersection_of": [
            "ECO:0001011 ! annexin-V staining evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001011 ! annexin-V staining evidence"
        ],
        "name": [
            "annexin-V staining evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001149": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T15:53:38Z"
        ],
        "def": [
            "\"A type of behavioral assay evidence used that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001149"
        ],
        "intersection_of": [
            "ECO:0001012 ! behavioral assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001012 ! behavioral assay evidence"
        ],
        "name": [
            "behavioral assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001150": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T15:54:44Z"
        ],
        "def": [
            "\"A type of blocking monoclonal antibody evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001150"
        ],
        "intersection_of": [
            "ECO:0001013 ! blocking monoclonal antibody evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000353 {is_inferred=\"true\"} ! physical interaction evidence used in manual assertion",
            "ECO:0001013 ! blocking monoclonal antibody evidence"
        ],
        "name": [
            "blocking monoclonal antibody evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001151": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T15:55:33Z"
        ],
        "def": [
            "\"A type of blocking peptide evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001151"
        ],
        "intersection_of": [
            "ECO:0001014 ! blocking peptide evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000353 {is_inferred=\"true\"} ! physical interaction evidence used in manual assertion",
            "ECO:0001014 ! blocking peptide evidence"
        ],
        "name": [
            "blocking peptide evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001152": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T16:01:39Z"
        ],
        "def": [
            "\"A type of blocking polyclonal antibody evidence tthat is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001152"
        ],
        "intersection_of": [
            "ECO:0001015 ! blocking polyclonal antibody evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000353 {is_inferred=\"true\"} ! physical interaction evidence used in manual assertion",
            "ECO:0001015 ! blocking polyclonal antibody evidence"
        ],
        "name": [
            "blocking polyclonal antibody evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001153": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T16:02:47Z"
        ],
        "def": [
            "\"A type of blood test evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001153"
        ],
        "intersection_of": [
            "ECO:0001016 ! blood test evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001016 ! blood test evidence"
        ],
        "name": [
            "blood test evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001154": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T16:03:53Z"
        ],
        "def": [
            "\"A type of Boyden chamber assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001154"
        ],
        "intersection_of": [
            "ECO:0001017 ! Boyden chamber assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001017 ! Boyden chamber assay evidence"
        ],
        "name": [
            "Boyden chamber assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001155": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T16:04:49Z"
        ],
        "def": [
            "\"A type of BrdU incorporation assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001155"
        ],
        "intersection_of": [
            "ECO:0001018 ! bromodeoxyuridine incorporation assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0001018 ! bromodeoxyuridine incorporation assay evidence",
            "ECO:0001241 {is_inferred=\"true\"} ! nucleotide analog incorporation evidence used in manual assertion"
        ],
        "name": [
            "bromodeoxyuridine incorporation assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001156": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T16:06:06Z"
        ],
        "def": [
            "\"A type of caspase assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001156"
        ],
        "intersection_of": [
            "ECO:0001019 ! caspase assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001019 ! caspase assay evidence"
        ],
        "name": [
            "caspase assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001157": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T16:07:13Z"
        ],
        "def": [
            "\"A type of cell counting evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001157"
        ],
        "intersection_of": [
            "ECO:0001020 ! cell counting evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001020 ! cell counting evidence"
        ],
        "name": [
            "cell counting evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001158": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T16:07:58Z"
        ],
        "def": [
            "\"A type of cell permeability assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001158"
        ],
        "intersection_of": [
            "ECO:0001021 ! cell permeability assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001021 ! cell permeability assay evidence"
        ],
        "name": [
            "cell permeability assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001159": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T16:08:54Z"
        ],
        "def": [
            "\"A type of carboxyfluorescein diacetate succinimidyl ester staining evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001159"
        ],
        "intersection_of": [
            "ECO:0001022 ! carboxyfluorescein diacetate succinimidyl ester staining evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001022 ! carboxyfluorescein diacetate succinimidyl ester staining evidence"
        ],
        "name": [
            "carboxyfluorescein diacetate succinimidyl ester staining evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001160": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T16:09:55Z"
        ],
        "def": [
            "\"A type of chemiluminescence-linked immunoassay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001160"
        ],
        "intersection_of": [
            "ECO:0001023 ! chemiluminescence-linked immunoassay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001023 ! chemiluminescence-linked immunoassay evidence"
        ],
        "name": [
            "chemiluminescence-linked immunoassay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001161": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T16:10:40Z"
        ],
        "def": [
            "\"A type of chimeric protein evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001161"
        ],
        "intersection_of": [
            "ECO:0001024 ! chimeric protein evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000315 {is_inferred=\"true\"} ! mutant phenotype evidence used in manual assertion",
            "ECO:0001024 ! chimeric protein evidence"
        ],
        "name": [
            "chimeric protein evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001162": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T16:11:22Z"
        ],
        "def": [
            "\"A type of co-electrophoresis evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001162"
        ],
        "intersection_of": [
            "ECO:0001025 ! co-electrophoresis evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001025 ! co-electrophoresis evidence"
        ],
        "name": [
            "co-electrophoresis evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001163": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T16:12:04Z"
        ],
        "def": [
            "\"A type of co-localization evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001163"
        ],
        "intersection_of": [
            "ECO:0001026 ! co-localization evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0001026 ! co-localization evidence",
            "ECO:0001256 {is_inferred=\"true\"} ! imaging assay evidence used in manual assertion"
        ],
        "name": [
            "co-localization evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001164": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T16:12:55Z"
        ],
        "def": [
            "\"A type of co-sedimentation assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001164"
        ],
        "intersection_of": [
            "ECO:0001028 ! co-sedimentation assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000353 {is_inferred=\"true\"} ! physical interaction evidence used in manual assertion",
            "ECO:0001028 ! co-sedimentation assay evidence"
        ],
        "name": [
            "co-sedimentation assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001165": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T16:14:57Z"
        ],
        "def": [
            "\"A type of colony counting evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001165"
        ],
        "intersection_of": [
            "ECO:0001027 ! colony counting evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001027 ! colony counting evidence"
        ],
        "name": [
            "colony counting evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001166": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T16:16:01Z"
        ],
        "def": [
            "\"A type of comet assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001166"
        ],
        "intersection_of": [
            "ECO:0001029 ! comet assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001029 ! comet assay evidence"
        ],
        "name": [
            "comet assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001167": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T16:17:22Z"
        ],
        "def": [
            "\"A type of conditional knockin evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001167"
        ],
        "intersection_of": [
            "ECO:0001031 ! conditional knockin evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0001031 ! conditional knockin evidence",
            "ECO:0001224 {is_inferred=\"true\"} ! knockin evidence used in manual assertion"
        ],
        "name": [
            "conditional knockin evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001168": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T16:17:58Z"
        ],
        "def": [
            "\"A type of conditional knockout evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001168"
        ],
        "intersection_of": [
            "ECO:0001030 ! conditional knockout evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0001030 ! conditional knockout evidence",
            "ECO:0001225 {is_inferred=\"true\"} ! knockout evidence used in manual assertion"
        ],
        "name": [
            "conditional knockout evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001169": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T16:20:23Z"
        ],
        "def": [
            "\"A type of constitutively active mutant evidence that is used a in manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001169"
        ],
        "intersection_of": [
            "ECO:0001032 ! constitutively active mutant evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001032 ! constitutively active mutant evidence"
        ],
        "name": [
            "constitutively active mutant evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001170": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T16:21:29Z"
        ],
        "def": [
            "\"A type of cross-linking evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001170"
        ],
        "intersection_of": [
            "ECO:0001033 ! cross-linking evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0000353 {is_inferred=\"true\"} ! physical interaction evidence used in manual assertion",
            "ECO:0001033 ! cross-linking evidence"
        ],
        "name": [
            "cross-linking evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001171": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T16:22:20Z"
        ],
        "def": [
            "\"A type of crystallography evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001171"
        ],
        "intersection_of": [
            "ECO:0001034 ! crystallography evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001034 ! crystallography evidence"
        ],
        "name": [
            "crystallography evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001172": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T16:23:10Z"
        ],
        "def": [
            "\"A type of cytochemistry evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001172"
        ],
        "intersection_of": [
            "ECO:0001035 ! cytochemistry evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000270 {is_inferred=\"true\"} ! expression evidence used in manual assertion",
            "ECO:0001035 ! cytochemistry evidence"
        ],
        "name": [
            "cytochemistry evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001173": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T16:23:53Z"
        ],
        "def": [
            "\"A type of cytochrome C release assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001173"
        ],
        "intersection_of": [
            "ECO:0001036 ! cytochrome C release assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001036 ! cytochrome C release assay evidence"
        ],
        "name": [
            "cytochrome C release assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001174": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T16:25:03Z"
        ],
        "def": [
            "\"A type of 4',6-diamidino-2-phenylindole staining evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001174"
        ],
        "intersection_of": [
            "ECO:0001037 ! 4',6-diamidino-2-phenylindole staining evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001037 ! 4',6-diamidino-2-phenylindole staining evidence"
        ],
        "name": [
            "4',6-diamidino-2-phenylindole staining evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001175": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T16:25:54Z"
        ],
        "def": [
            "\"A type of deletion evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001175"
        ],
        "intersection_of": [
            "ECO:0001038 ! deletion evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001038 ! deletion evidence"
        ],
        "name": [
            "deletion evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001176": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T16:26:46Z"
        ],
        "def": [
            "\"A type of DNA laddering assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001176"
        ],
        "intersection_of": [
            "ECO:0001039 ! DNA laddering assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001039 ! DNA laddering assay evidence"
        ],
        "name": [
            "DNA laddering assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001177": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T16:45:52Z"
        ],
        "def": [
            "\"A type of RNA dot blot assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001177"
        ],
        "intersection_of": [
            "ECO:0001040 ! RNA dot blot assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001040 ! RNA dot blot assay evidence"
        ],
        "name": [
            "RNA dot blot assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001178": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T16:46:50Z"
        ],
        "def": [
            "\"A type of RNA microarray evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001178"
        ],
        "intersection_of": [
            "ECO:0001041 ! RNA microarray evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001041 ! RNA microarray evidence"
        ],
        "name": [
            "RNA microarray evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001179": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T16:47:44Z"
        ],
        "def": [
            "\"A type of dominant-negative mutant evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001179"
        ],
        "intersection_of": [
            "ECO:0001042 ! dominant-negative mutant evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001042 ! dominant-negative mutant evidence"
        ],
        "name": [
            "dominant-negative mutant evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001180": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T16:59:39Z"
        ],
        "def": [
            "\"A type of eTag assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001180"
        ],
        "intersection_of": [
            "ECO:0001045 ! eTag assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000353 {is_inferred=\"true\"} ! physical interaction evidence used in manual assertion",
            "ECO:0001045 ! eTag assay evidence"
        ],
        "name": [
            "eTag assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001181": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:03:28Z"
        ],
        "def": [
            "\"A type of filter binding assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001181"
        ],
        "intersection_of": [
            "ECO:0001046 ! filter binding assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000353 {is_inferred=\"true\"} ! physical interaction evidence used in manual assertion",
            "ECO:0001046 ! filter binding assay evidence"
        ],
        "name": [
            "filter binding assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001182": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:04:20Z"
        ],
        "def": [
            "\"A type of fluorescence in situ hybridization evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001182"
        ],
        "intersection_of": [
            "ECO:0001047 ! fluorescence in situ hybridization evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000270 {is_inferred=\"true\"} ! expression evidence used in manual assertion",
            "ECO:0001047 ! fluorescence in situ hybridization evidence"
        ],
        "name": [
            "fluorescence in situ hybridization evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001183": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:05:22Z"
        ],
        "def": [
            "\"A type of fluorescence resonance energy transfer evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001183"
        ],
        "intersection_of": [
            "ECO:0001048 ! fluorescence resonance energy transfer evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000353 {is_inferred=\"true\"} ! physical interaction evidence used in manual assertion",
            "ECO:0001048 ! fluorescence resonance energy transfer evidence"
        ],
        "name": [
            "fluorescence resonance energy transfer evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001184": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:06:14Z"
        ],
        "def": [
            "\"A type of gel-filtration evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001184"
        ],
        "intersection_of": [
            "ECO:0001049 ! gel-filtration evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001049 ! gel-filtration evidence"
        ],
        "name": [
            "gel-filtration evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001185": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:07:19Z"
        ],
        "def": [
            "\"A type of histochemistry evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001185"
        ],
        "intersection_of": [
            "ECO:0001050 ! histochemistry evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000270 {is_inferred=\"true\"} ! expression evidence used in manual assertion",
            "ECO:0001050 ! histochemistry evidence"
        ],
        "name": [
            "histochemistry evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001186": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:12:39Z"
        ],
        "def": [
            "\"A type of immunocytochemistry evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001186"
        ],
        "intersection_of": [
            "ECO:0001053 ! immunocytochemistry evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001053 ! immunocytochemistry evidence"
        ],
        "name": [
            "immunocytochemistry evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001187": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:09:50Z"
        ],
        "def": [
            "\"A type of histology evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001187"
        ],
        "intersection_of": [
            "ECO:0001051 ! histology evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0001051 ! histology evidence",
            "ECO:0001256 {is_inferred=\"true\"} ! imaging assay evidence used in manual assertion"
        ],
        "name": [
            "histology evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001188": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:16:19Z"
        ],
        "def": [
            "\"A type of immunodepletion evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001188"
        ],
        "intersection_of": [
            "ECO:0001054 ! immunodepletion evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000353 {is_inferred=\"true\"} ! physical interaction evidence used in manual assertion",
            "ECO:0001054 ! immunodepletion evidence"
        ],
        "name": [
            "immunodepletion evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001189": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:17:22Z"
        ],
        "def": [
            "\"A type of immunohistochemistry evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001189"
        ],
        "intersection_of": [
            "ECO:0001055 ! immunohistochemistry evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001055 ! immunohistochemistry evidence"
        ],
        "name": [
            "immunohistochemistry evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001190": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:19:24Z"
        ],
        "def": [
            "\"A type of in vitro acetylation assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001190"
        ],
        "intersection_of": [
            "ECO:0001057 ! in vitro acetylation assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001057 ! in vitro acetylation assay evidence"
        ],
        "name": [
            "in vitro acetylation assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001191": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:21:22Z"
        ],
        "def": [
            "\"A type of in vitro cleavage assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001191"
        ],
        "intersection_of": [
            "ECO:0001058 ! in vitro cleavage assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001058 ! in vitro cleavage assay evidence"
        ],
        "name": [
            "in vitro cleavage assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001192": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:23:42Z"
        ],
        "def": [
            "\"A type of in vitro deacetylation assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001192"
        ],
        "intersection_of": [
            "ECO:0001060 ! in vitro deacetylation assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001060 ! in vitro deacetylation assay evidence"
        ],
        "name": [
            "in vitro deacetylation assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001193": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:25:45Z"
        ],
        "def": [
            "\"A type of in vitro defarnesylation assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001193"
        ],
        "intersection_of": [
            "ECO:0001061 ! in vitro defarnesylation assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001061 ! in vitro defarnesylation assay evidence"
        ],
        "name": [
            "in vitro defarnesylation assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001194": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:27:00Z"
        ],
        "def": [
            "\"A type of in vitro demethylation assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001194"
        ],
        "intersection_of": [
            "ECO:0001062 ! in vitro demethylation assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001062 ! in vitro demethylation assay evidence"
        ],
        "name": [
            "in vitro demethylation assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001195": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:29:01Z"
        ],
        "def": [
            "\"A type of in vitro desumoylation assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001195"
        ],
        "intersection_of": [
            "ECO:0001063 ! in vitro desumoylation assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001063 ! in vitro desumoylation assay evidence"
        ],
        "name": [
            "in vitro desumoylation assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001196": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:29:48Z"
        ],
        "def": [
            "\"A type of in vitro deubiquitination assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001196"
        ],
        "intersection_of": [
            "ECO:0001059 ! in vitro deubiquitination assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001059 ! in vitro deubiquitination assay evidence"
        ],
        "name": [
            "in vitro deubiquitination assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001197": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:30:50Z"
        ],
        "def": [
            "\"A type of in vitro farnesylation assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001197"
        ],
        "intersection_of": [
            "ECO:0001064 ! in vitro farnesylation assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001064 ! in vitro farnesylation assay evidence"
        ],
        "name": [
            "in vitro farnesylation assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001198": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:31:54Z"
        ],
        "def": [
            "\"A type of in vitro methylation assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001198"
        ],
        "intersection_of": [
            "ECO:0001065 ! in vitro methylation assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001065 ! in vitro methylation assay evidence"
        ],
        "name": [
            "in vitro methylation assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001199": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:32:57Z"
        ],
        "def": [
            "\"A type of in vitro palmitoylation assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001199"
        ],
        "intersection_of": [
            "ECO:0001066 ! in vitro palmitoylation assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001066 ! in vitro palmitoylation assay evidence"
        ],
        "name": [
            "in vitro palmitoylation assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001200": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:34:16Z"
        ],
        "def": [
            "\"A type of in vitro phosphatase assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001200"
        ],
        "intersection_of": [
            "ECO:0001067 ! in vitro phosphatase assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001067 ! in vitro phosphatase assay evidence"
        ],
        "name": [
            "in vitro phosphatase assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001201": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:34:57Z"
        ],
        "def": [
            "\"A type of in vitro polyADP-ribosylation assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001201"
        ],
        "intersection_of": [
            "ECO:0001069 ! in vitro polyADP-ribosylation assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001069 ! in vitro polyADP-ribosylation assay evidence"
        ],
        "name": [
            "in vitro polyADP-ribosylation assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001202": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:39:57Z"
        ],
        "def": [
            "\"A type of in vitro protein kinase assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001202"
        ],
        "intersection_of": [
            "ECO:0001068 ! in vitro protein kinase assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001068 ! in vitro protein kinase assay evidence"
        ],
        "name": [
            "in vitro protein kinase assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001203": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:41:25Z"
        ],
        "def": [
            "\"A type of in vitro sumoylation assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001203"
        ],
        "intersection_of": [
            "ECO:0001070 ! in vitro sumoylation assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001070 ! in vitro sumoylation assay evidence"
        ],
        "name": [
            "in vitro sumoylation assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001204": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:44:40Z"
        ],
        "def": [
            "\"A type of in vitro transcription assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001204"
        ],
        "intersection_of": [
            "ECO:0001071 ! in vitro transcription assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001071 ! in vitro transcription assay evidence"
        ],
        "name": [
            "in vitro transcription assay used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001205": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:46:30Z"
        ],
        "def": [
            "\"A type of in vitro translation assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001205"
        ],
        "intersection_of": [
            "ECO:0001072 ! in vitro translation assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001072 ! in vitro translation assay evidence"
        ],
        "name": [
            "in vitro translation assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001206": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:47:18Z"
        ],
        "def": [
            "\"A type of in vitro ubiquitination assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001206"
        ],
        "intersection_of": [
            "ECO:0001073 ! in vitro ubiquitination assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001073 ! in vitro ubiquitination assay evidence"
        ],
        "name": [
            "in vitro ubiquitination assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001207": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:48:56Z"
        ],
        "def": [
            "\"A type of in vivo acetylation assay evidence that is used in manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001207"
        ],
        "intersection_of": [
            "ECO:0001074 ! in vivo acetylation assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001074 ! in vivo acetylation assay evidence"
        ],
        "name": [
            "in vivo acetylation assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001208": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:49:43Z"
        ],
        "def": [
            "\"A type of in vivo cleavage assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001208"
        ],
        "intersection_of": [
            "ECO:0001075 ! in vivo cleavage assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001075 ! in vivo cleavage assay evidence"
        ],
        "name": [
            "in vivo cleavage assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001209": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:50:47Z"
        ],
        "def": [
            "\"A type of in vivo deacetylation assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001209"
        ],
        "intersection_of": [
            "ECO:0001076 ! in vivo deacetylation assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001076 ! in vivo deacetylation assay evidence"
        ],
        "name": [
            "in vivo deacetylation assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001210": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:52:41Z"
        ],
        "def": [
            "\"A type of in vivo defarnesylation assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001210"
        ],
        "intersection_of": [
            "ECO:0001077 ! in vivo defarnesylation assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001077 ! in vivo defarnesylation assay evidence"
        ],
        "name": [
            "in vivo defarnesylation assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001211": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:53:40Z"
        ],
        "def": [
            "\"A type of in vivo demethylation assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001211"
        ],
        "intersection_of": [
            "ECO:0001078 ! in vivo demethylation assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001078 ! in vivo demethylation assay evidence"
        ],
        "name": [
            "in vivo demethylation assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001212": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:54:36Z"
        ],
        "def": [
            "\"A type of in vivo desumoylation assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001212"
        ],
        "intersection_of": [
            "ECO:0001080 ! in vivo desumoylation assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001080 ! in vivo desumoylation assay evidence"
        ],
        "name": [
            "in vivo desumoylation assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001213": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:56:28Z"
        ],
        "def": [
            "\"A type of in vivo deubiquitination assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001213"
        ],
        "intersection_of": [
            "ECO:0001079 ! in vivo deubiquitination assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001079 ! in vivo deubiquitination assay evidence"
        ],
        "name": [
            "in vivo deubiquitination assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001214": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T17:57:18Z"
        ],
        "def": [
            "\"A type of in vivo farnesylation assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001214"
        ],
        "intersection_of": [
            "ECO:0001081 ! in vivo farnesylation assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001081 ! in vivo farnesylation assay evidence"
        ],
        "name": [
            "in vivo farnesylation assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001215": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T18:03:16Z"
        ],
        "def": [
            "\"A type of in vivo methylation assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001215"
        ],
        "intersection_of": [
            "ECO:0001082 ! in vivo methylation assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001082 ! in vivo methylation assay evidence"
        ],
        "name": [
            "in vivo methylation assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001216": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T18:04:07Z"
        ],
        "def": [
            "\"A type of in vivo palmitoylation assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001216"
        ],
        "intersection_of": [
            "ECO:0001083 ! in vivo palmitoylation assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001083 ! in vivo palmitoylation assay evidence"
        ],
        "name": [
            "in vivo palmitoylation assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001217": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T18:06:08Z"
        ],
        "def": [
            "\"A type of in vivo phosphatase assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001217"
        ],
        "intersection_of": [
            "ECO:0001084 ! in vivo phosphatase assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001084 ! in vivo phosphatase assay evidence"
        ],
        "name": [
            "in vivo phosphatase assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001218": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T18:07:58Z"
        ],
        "def": [
            "\"A type of in vivo protein kinase assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001218"
        ],
        "intersection_of": [
            "ECO:0001085 ! in vivo protein kinase assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001085 ! in vivo protein kinase assay evidence"
        ],
        "name": [
            "in vivo protein kinase assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001219": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T18:09:49Z"
        ],
        "def": [
            "\"A type of in vivo sumoylation assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001219"
        ],
        "intersection_of": [
            "ECO:0001086 ! in vivo sumoylation assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001086 ! in vivo sumoylation assay evidence"
        ],
        "name": [
            "in vivo sumoylation assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001220": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T18:11:20Z"
        ],
        "def": [
            "\"A type of in vivo transcription assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001220"
        ],
        "intersection_of": [
            "ECO:0001087 ! in vivo transcription assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001087 ! in vivo transcription assay evidence"
        ],
        "name": [
            "in vivo transcription assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001221": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T18:42:50Z"
        ],
        "def": [
            "\"A type of in vivo translation assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001221"
        ],
        "intersection_of": [
            "ECO:0001088 ! in vivo translation assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001088 ! in vivo translation assay evidence"
        ],
        "name": [
            "in vivo translation assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001222": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T18:44:11Z"
        ],
        "def": [
            "\"A type of in vivo ubiquitination assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001222"
        ],
        "intersection_of": [
            "ECO:0001089 ! in vivo ubiquitination assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001089 ! in vivo ubiquitination assay evidence"
        ],
        "name": [
            "in vivo ubiquitination assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001223": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T18:45:56Z"
        ],
        "def": [
            "\"A type of induced mutation evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001223"
        ],
        "intersection_of": [
            "ECO:0001056 ! induced mutation evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001056 ! induced mutation evidence"
        ],
        "name": [
            "induced mutation evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001224": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T18:49:35Z"
        ],
        "def": [
            "\"A type of knockin evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001224"
        ],
        "intersection_of": [
            "ECO:0001090 ! knockin evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001090 ! knockin evidence"
        ],
        "name": [
            "knockin evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001225": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T18:51:31Z"
        ],
        "def": [
            "\"A type of knockout evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001225"
        ],
        "intersection_of": [
            "ECO:0001091 ! knockout evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001091 ! knockout evidence"
        ],
        "name": [
            "knockout evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001226": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T18:52:19Z"
        ],
        "def": [
            "\"A type of lipid binding assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001226"
        ],
        "intersection_of": [
            "ECO:0001092 ! lipid binding assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000353 {is_inferred=\"true\"} ! physical interaction evidence used in manual assertion",
            "ECO:0001092 ! lipid binding assay evidence"
        ],
        "name": [
            "lipid binding assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001227": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T18:55:16Z"
        ],
        "def": [
            "\"A type of LUMIER assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001227"
        ],
        "intersection_of": [
            "ECO:0001093 ! luminescence-based mammalian interactome mapping assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000353 {is_inferred=\"true\"} ! physical interaction evidence used in manual assertion",
            "ECO:0001093 ! luminescence-based mammalian interactome mapping assay evidence"
        ],
        "name": [
            "luminescence-based mammalian interactome mapping assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001228": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T18:56:21Z"
        ],
        "def": [
            "\"A type of macroscopy evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001228"
        ],
        "intersection_of": [
            "ECO:0001094 ! macroscopy evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001094 ! macroscopy evidence"
        ],
        "name": [
            "macroscopy evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001229": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T18:57:25Z"
        ],
        "def": [
            "\"A type of mammalian 2-hybrid assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001229"
        ],
        "intersection_of": [
            "ECO:0001095 ! mammalian 2-hybrid assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000353 {is_inferred=\"true\"} ! physical interaction evidence used in manual assertion",
            "ECO:0001095 ! mammalian 2-hybrid assay evidence"
        ],
        "name": [
            "mammalian 2-hybrid assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001230": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T18:59:50Z"
        ],
        "def": [
            "\"A type of mass spectrometry evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001230"
        ],
        "intersection_of": [
            "ECO:0001096 ! mass spectrometry evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001096 ! mass spectrometry evidence"
        ],
        "name": [
            "mass spectrometry evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001231": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T19:04:43Z"
        ],
        "def": [
            "\"A type of medical imaging evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001231"
        ],
        "intersection_of": [
            "ECO:0001097 ! medical imaging evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0001097 ! medical imaging evidence",
            "ECO:0001256 {is_inferred=\"true\"} ! imaging assay evidence used in manual assertion"
        ],
        "name": [
            "medical imaging evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001232": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T19:05:37Z"
        ],
        "def": [
            "\"A type of microscopy evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001232"
        ],
        "intersection_of": [
            "ECO:0001098 ! microscopy evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0001098 ! microscopy evidence",
            "ECO:0001256 {is_inferred=\"true\"} ! imaging assay evidence used in manual assertion"
        ],
        "name": [
            "microscopy evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001233": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T19:08:13Z"
        ],
        "def": [
            "\"A type of motility wound healing assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001233"
        ],
        "intersection_of": [
            "ECO:0001099 ! motility wound healing assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001099 ! motility wound healing assay evidence"
        ],
        "name": [
            "motility wound healing assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001234": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T19:08:51Z"
        ],
        "def": [
            "\"A type of MTS assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001234"
        ],
        "intersection_of": [
            "ECO:0001100 ! MTS assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001100 ! MTS assay evidence"
        ],
        "name": [
            "MTS assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001235": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T19:13:17Z"
        ],
        "def": [
            "\"A type of MTT assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001235"
        ],
        "intersection_of": [
            "ECO:0001101 ! MTT assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001101 ! MTT assay evidence"
        ],
        "name": [
            "MTT assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001236": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T19:14:03Z"
        ],
        "def": [
            "\"A type of multiplex bead-based immunoassay evidence that is  used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001236"
        ],
        "intersection_of": [
            "ECO:0001102 ! multiplex bead-based immunoassay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001102 ! multiplex bead-based immunoassay evidence"
        ],
        "name": [
            "multiplex bead-based immunoassay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001237": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T19:15:48Z"
        ],
        "def": [
            "\"A type of natural variation mutant evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001237"
        ],
        "intersection_of": [
            "ECO:0001103 ! natural variation mutant evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001103 ! natural variation mutant evidence"
        ],
        "name": [
            "natural variation mutant evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001238": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T19:18:50Z"
        ],
        "def": [
            "\"A type of nuclear magnetic resonance spectroscopy evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001238"
        ],
        "intersection_of": [
            "ECO:0001105 ! nuclear magnetic resonance spectroscopy evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001105 ! nuclear magnetic resonance spectroscopy evidence"
        ],
        "name": [
            "nuclear magnetic resonance spectroscopy evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001239": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T19:19:55Z"
        ],
        "def": [
            "\"A type of nuclear fragmentation evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001239"
        ],
        "intersection_of": [
            "ECO:0001104 ! nuclear fragmentation evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001104 ! nuclear fragmentation evidence"
        ],
        "name": [
            "nuclear fragmentation evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001240": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T19:23:41Z"
        ],
        "def": [
            "\"A type of nuclease protection assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001240"
        ],
        "intersection_of": [
            "ECO:0001106 ! nuclease protection assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001106 ! nuclease protection assay evidence"
        ],
        "name": [
            "nuclease protection assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001241": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T19:25:17Z"
        ],
        "def": [
            "\"A type of nucleotide analog incorporation evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001241"
        ],
        "intersection_of": [
            "ECO:0001107 ! nucleotide analog incorporation evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001107 ! nucleotide analog incorporation evidence"
        ],
        "name": [
            "nucleotide analog incorporation evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001242": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T19:27:17Z"
        ],
        "def": [
            "\"A type of phage display evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001242"
        ],
        "intersection_of": [
            "ECO:0001108 ! phage display evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0000353 {is_inferred=\"true\"} ! physical interaction evidence used in manual assertion",
            "ECO:0001108 ! phage display evidence"
        ],
        "name": [
            "phage display evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001243": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T19:28:59Z"
        ],
        "def": [
            "\"A type of phosphoamino acid analysis evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001243"
        ],
        "intersection_of": [
            "ECO:0001109 ! phosphoamino acid analysis evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001109 ! phosphoamino acid analysis evidence"
        ],
        "name": [
            "phosphoamino acid analysis evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001244": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T19:30:13Z"
        ],
        "def": [
            "\"A type of peptide affinity enrichment evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001244"
        ],
        "intersection_of": [
            "ECO:0001110 ! peptide affinity enrichment evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000353 {is_inferred=\"true\"} ! physical interaction evidence used in manual assertion",
            "ECO:0001110 ! peptide affinity enrichment evidence"
        ],
        "name": [
            "peptide affinity enrichment evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001245": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T20:16:45Z"
        ],
        "def": [
            "\"A type of peptide array evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001245"
        ],
        "intersection_of": [
            "ECO:0001112 ! peptide array evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001112 ! peptide array evidence"
        ],
        "name": [
            "peptide array evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001246": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T20:17:27Z"
        ],
        "def": [
            "\"A type of physical examination evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001246"
        ],
        "intersection_of": [
            "ECO:0001111 ! physical examination evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001111 ! physical examination evidence"
        ],
        "name": [
            "physical examination evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001247": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T20:18:10Z"
        ],
        "def": [
            "\"A type of point mutation evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001247"
        ],
        "intersection_of": [
            "ECO:0001113 ! point mutation evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001113 ! point mutation evidence"
        ],
        "name": [
            "point mutation evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001248": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T20:18:58Z"
        ],
        "def": [
            "\"A type of propidium iodide staining evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001248"
        ],
        "intersection_of": [
            "ECO:0001114 ! propidium iodide staining evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001114 ! propidium iodide staining evidence"
        ],
        "name": [
            "propidium iodide staining evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001249": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T20:19:35Z"
        ],
        "def": [
            "\"A type of protein detection by fluorescence evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001249"
        ],
        "intersection_of": [
            "ECO:0001115 ! fluorescence evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001115 ! fluorescence evidence"
        ],
        "name": [
            "fluorescence evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001250": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T20:20:30Z"
        ],
        "def": [
            "\"A type of protein dot blot assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001250"
        ],
        "intersection_of": [
            "ECO:0001116 ! protein dot blot assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001116 ! protein dot blot assay evidence"
        ],
        "name": [
            "protein dot blot assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001251": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T20:21:11Z"
        ],
        "def": [
            "\"A type of protein microarray evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001251"
        ],
        "intersection_of": [
            "ECO:0001117 ! protein microarray evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001117 ! protein microarray evidence"
        ],
        "name": [
            "protein microarray evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001252": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T20:22:08Z"
        ],
        "def": [
            "\"A type of protein sequencing assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001252"
        ],
        "intersection_of": [
            "ECO:0001118 ! protein sequencing assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001118 ! protein sequencing assay evidence"
        ],
        "name": [
            "protein sequencing assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001253": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T20:23:15Z"
        ],
        "def": [
            "\"A type of quantitative mass spectrometry evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001253"
        ],
        "intersection_of": [
            "ECO:0001119 ! quantitative mass spectrometry evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0001119 ! quantitative mass spectrometry evidence",
            "ECO:0001230 {is_inferred=\"true\"} ! mass spectrometry evidence used in manual assertion"
        ],
        "name": [
            "quantitative mass spectrometry evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001254": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T20:27:05Z"
        ],
        "def": [
            "\"A type of radioisotope assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001254"
        ],
        "intersection_of": [
            "ECO:0001120 ! radioisotope assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001120 ! radioisotope assay evidence"
        ],
        "name": [
            "radioisotope assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001255": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T20:28:04Z"
        ],
        "def": [
            "\"A type of radioimmunoassay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001255"
        ],
        "intersection_of": [
            "ECO:0001121 ! radioimmunoassay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001121 ! radioimmunoassay evidence"
        ],
        "name": [
            "radioimmunoassay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001256": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T20:29:00Z"
        ],
        "def": [
            "\"A type of imaging assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001256"
        ],
        "intersection_of": [
            "ECO:0000324 ! imaging assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0000324 ! imaging assay evidence"
        ],
        "name": [
            "imaging assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001257": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T20:29:05Z"
        ],
        "def": [
            "\"A type of restriction fragment detection evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001257"
        ],
        "intersection_of": [
            "ECO:0001124 ! restriction fragment detection evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001124 ! restriction fragment detection evidence"
        ],
        "name": [
            "restriction fragment detection evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001258": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T20:29:09Z"
        ],
        "def": [
            "\"A type of spectrophotometry evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001258"
        ],
        "intersection_of": [
            "ECO:0001126 ! spectrophotometry evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001126 ! spectrophotometry evidence"
        ],
        "name": [
            "spectrophotometry evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001259": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T20:29:13Z"
        ],
        "def": [
            "\"A type of syngeneic transplantation experiment evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001259"
        ],
        "intersection_of": [
            "ECO:0001128 ! syngeneic transplantation experiment evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001128 ! syngeneic transplantation experiment evidence"
        ],
        "name": [
            "syngeneic transplantation experiment evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001260": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T20:29:18Z"
        ],
        "def": [
            "\"A type of xenotransplantation experiment evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001260"
        ],
        "intersection_of": [
            "ECO:0001137 ! xenotransplantation experiment evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001137 ! xenotransplantation experiment evidence"
        ],
        "name": [
            "xenotransplantation experiment evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001261": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T20:29:22Z"
        ],
        "def": [
            "\"A type of WST-1 assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001261"
        ],
        "intersection_of": [
            "ECO:0001136 ! WST-1 assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001136 ! WST-1 assay evidence"
        ],
        "name": [
            "WST-1 assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001262": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T20:29:26Z"
        ],
        "def": [
            "\"A type of wound healing assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001262"
        ],
        "intersection_of": [
            "ECO:0001135 ! wound healing assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001135 ! wound healing assay evidence"
        ],
        "name": [
            "wound healing assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001263": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T20:29:30Z"
        ],
        "def": [
            "\"A type of urine test evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001263"
        ],
        "intersection_of": [
            "ECO:0001134 ! urine test evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001134 ! urine test evidence"
        ],
        "name": [
            "urine test evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001264": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T20:29:34Z"
        ],
        "def": [
            "\"A type of TUNEL assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001264"
        ],
        "intersection_of": [
            "ECO:0001133 ! terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001133 ! terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence"
        ],
        "name": [
            "terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001265": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T20:29:38Z"
        ],
        "def": [
            "\"A type of tryptic phosphopeptide mapping assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001265"
        ],
        "intersection_of": [
            "ECO:0001132 ! tryptic phosphopeptide mapping assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0001132 ! tryptic phosphopeptide mapping assay evidence",
            "ECO:0001230 {is_inferred=\"true\"} ! mass spectrometry evidence used in manual assertion"
        ],
        "name": [
            "tryptic phosphopeptide mapping assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001266": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T20:29:41Z"
        ],
        "def": [
            "\"A type of transgenic organism evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001266"
        ],
        "intersection_of": [
            "ECO:0001131 ! transgenic organism evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001131 ! transgenic organism evidence"
        ],
        "name": [
            "transgenic organism evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001267": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T20:29:45Z"
        ],
        "def": [
            "\"A type of tissue microarray evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001267"
        ],
        "intersection_of": [
            "ECO:0001130 ! tissue microarray evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000271 {is_inferred=\"true\"} ! array experiment evidence used in manual assertion",
            "ECO:0001130 ! tissue microarray evidence"
        ],
        "name": [
            "tissue microarray evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001268": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T20:29:49Z"
        ],
        "def": [
            "\"A type of TACE activity assay evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001268"
        ],
        "intersection_of": [
            "ECO:0001129 ! TACE activity assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001129 ! TACE activity assay evidence"
        ],
        "name": [
            "TACE activity assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001269": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T20:29:54Z"
        ],
        "def": [
            "\"A type of surface plasmon resonance evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001269"
        ],
        "intersection_of": [
            "ECO:0001127 ! surface plasmon resonance evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000353 {is_inferred=\"true\"} ! physical interaction evidence used in manual assertion",
            "ECO:0001127 ! surface plasmon resonance evidence"
        ],
        "name": [
            "surface plasmon resonance evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001270": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T20:29:58Z"
        ],
        "def": [
            "\"A type of restriction landmark genome scanning evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001270"
        ],
        "intersection_of": [
            "ECO:0000089 ! restriction landmark genomic scanning evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000089 ! restriction landmark genomic scanning evidence",
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion"
        ],
        "name": [
            "restriction landmark genomic scanning evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001271": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T20:30:02Z"
        ],
        "def": [
            "\"A type of resonant mirror biosensor evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001271"
        ],
        "intersection_of": [
            "ECO:0001123 ! resonant mirror biosensor evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0001123 ! resonant mirror biosensor evidence"
        ],
        "name": [
            "resonant mirror biosensor evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001272": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-16T20:49:54Z"
        ],
        "def": [
            "\"A type of high-performance liquid chromatography evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001272"
        ],
        "intersection_of": [
            "ECO:0001052 ! high-performance liquid chromatography evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0001052 ! high-performance liquid chromatography evidence"
        ],
        "name": [
            "high-performance liquid chromatography evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001273": {
        "created_by": [
            "mchibucos"
        ],
        "creation_date": [
            "2014-03-17T22:34:56Z"
        ],
        "def": [
            "\"A type of ectopic expression evidence that is used in a manual assertion.\" [ECO:MCC]"
        ],
        "id": [
            "ECO:0001273"
        ],
        "intersection_of": [
            "ECO:0000017 ! ectopic expression evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000017 ! ectopic expression evidence",
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion"
        ],
        "name": [
            "ectopic expression evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001801": {
        "comment": [
            "Alkaline phosphatase reporter assay produces a hybrid protein with alkaline phosphatase activity following transportation across the cellular membrane."
        ],
        "created_by": [
            "swolfish"
        ],
        "creation_date": [
            "2015-10-29T15:53:45Z"
        ],
        "def": [
            "\"A type of reporter gene assay evidence based on the fusion of selected genes with the phoA gene to express alkaline phosphatase in periplasmic space for protein tracing.\" [ECO:SW, PMID:11823238]"
        ],
        "id": [
            "ECO:0001801"
        ],
        "is_a": [
            "ECO:0000049 ! reporter gene assay evidence"
        ],
        "name": [
            "alkaline phosphatase reporter gene assay evidence"
        ],
        "synonym": [
            "\"SEAP reporter assay\" RELATED []",
            "\"Secreted Embryonic Alkaline Phosphatase reporter assay\" RELATED []"
        ]
    },
    "ECO:0001802": {
        "comment": [
            "The assay is often performed using a plasmid borne construction on a lacZ strain."
        ],
        "created_by": [
            "swolfish"
        ],
        "creation_date": [
            "2015-10-29T15:57:10Z"
        ],
        "def": [
            "\"A type of reporter gene assay evidence based on the fusion of the lacZ gene to a specific promoter for the expression of beta-galactosidase which will appear blue when grown on a X-gal medium.\" [ECO:SW, PMID:20439410]"
        ],
        "id": [
            "ECO:0001802"
        ],
        "is_a": [
            "ECO:0000049 ! reporter gene assay evidence"
        ],
        "name": [
            "beta-galactosidase reporter gene assay evidence"
        ],
        "synonym": [
            "\"beta-gal reporter gene assay\" EXACT []",
            "\"lacZ reporter gene assay\" RELATED []"
        ]
    },
    "ECO:0001803": {
        "comment": [
            "The amount of acetylated chloramphenicol is directly proportional to the amount of CAT enzyme present."
        ],
        "created_by": [
            "swolfish"
        ],
        "creation_date": [
            "2015-10-29T16:06:42Z"
        ],
        "def": [
            "\"A type of reporter gene assay evidence based on the fusion of the CAT gene to a specific promoter for the expression of chloramphenicol acetyltransferase which confers resistance to the chloramphenicol antibiotic.\" [ECO:SW, PMID:1630936]"
        ],
        "id": [
            "ECO:0001803"
        ],
        "is_a": [
            "ECO:0000049 ! reporter gene assay evidence"
        ],
        "name": [
            "chloramphenicol acetyltransferase reporter gene assay evidence"
        ],
        "synonym": [
            "\"CAT reporter gene assay\" EXACT [PMID:1630936]"
        ]
    },
    "ECO:0001804": {
        "created_by": [
            "swolfish"
        ],
        "creation_date": [
            "2015-10-29T16:11:02Z"
        ],
        "def": [
            "\"A type of reporter gene assay evidence where the beta-glucuronidase enzyme from Escherichia coli is used as the reporter to transform non-fluorescent substrates into fluorescents for detection.\" [ECO:SW]"
        ],
        "id": [
            "ECO:0001804"
        ],
        "is_a": [
            "ECO:0000049 ! reporter gene assay evidence"
        ],
        "name": [
            "beta-glucuronidase reporter gene assay evidence"
        ],
        "synonym": [
            "\"GUS reporter gene assay\" EXACT []"
        ]
    },
    "ECO:0001805": {
        "created_by": [
            "swolfish"
        ],
        "creation_date": [
            "2015-10-29T16:15:39Z"
        ],
        "def": [
            "\"A type of reporter gene assay evidence where luciferase, an oxidative enzyme, is used as the reporter to detect a gene product with bioluminescence.\" [ECO:SW, PMID:17623934]"
        ],
        "id": [
            "ECO:0001805"
        ],
        "is_a": [
            "ECO:0000049 ! reporter gene assay evidence"
        ],
        "name": [
            "luciferase reporter gene assay evidence"
        ]
    },
    "ECO:0001806": {
        "created_by": [
            "swolfish"
        ],
        "creation_date": [
            "2015-10-29T16:37:36Z"
        ],
        "def": [
            "\"A type of chromatin immunoprecipitation evidence that uses gama-exonuclease to digest TF-unbound DNA after ChIP for the identification of transcription factor binding site locations with high-resolution data.\" [ECO:SW, PMID:25249628]"
        ],
        "id": [
            "ECO:0001806"
        ],
        "is_a": [
            "ECO:0000226 ! chromatin immunoprecipitation evidence"
        ],
        "name": [
            "chromatin immunoprecipitation- exonuclease evidence"
        ],
        "synonym": [
            "\"ChIP-exo\" EXACT [PMID:25249628]"
        ]
    },
    "ECO:0001807": {
        "created_by": [
            "swolfish"
        ],
        "creation_date": [
            "2015-11-03T12:13:17Z"
        ],
        "def": [
            "\"A type of electrophoretic mobility shift that is used in a manual assertion.\" [ECO:SW]"
        ],
        "id": [
            "ECO:0001807"
        ],
        "intersection_of": [
            "ECO:0000096 ! electrophoretic mobility shift assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000096 ! electrophoretic mobility shift assay evidence",
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion"
        ],
        "name": [
            "electrophoretic mobility shift assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001808": {
        "created_by": [
            "swolfish"
        ],
        "creation_date": [
            "2015-11-03T12:22:46Z"
        ],
        "def": [
            "\"A type of reverse transcription polymerase chain reaction evidence that is used in a manual assertion.\" [ECO:SW]"
        ],
        "id": [
            "ECO:0001808"
        ],
        "intersection_of": [
            "ECO:0000109 ! reverse transcription polymerase chain reaction evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000109 ! reverse transcription polymerase chain reaction evidence",
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion"
        ],
        "name": [
            "reverse transcription polymerase chain reaction evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0001809": {
        "comment": [
            "Biotinylation is commonly used for labeling and beads are commonly used as the matrix. Bound protein will remain attached to the beads. Results can be detected through gel electrophoresis and then sequenced by mass-spectrometry. This technique can be used to demonstrate binding of a purified protein, or to purify the binding protein from crude extract or protein mixture."
        ],
        "created_by": [
            "swolfish"
        ],
        "creation_date": [
            "2015-11-03T13:48:49Z"
        ],
        "def": [
            "\"A type of chromatography evidence where immobilized promoter DNA is labeled and bound to a matrix through which a protein mixture is poured and then eluted to purify specific DNA binding proteins.\" [ECO:SW, PMID:11694305, PMID:11725488]"
        ],
        "id": [
            "ECO:0001809"
        ],
        "is_a": [
            "ECO:0000325 ! chromatography evidence"
        ],
        "name": [
            "DNA affinity chromatography evidence"
        ],
        "synonym": [
            "\"DNA affinity purification\" RELATED []"
        ]
    },
    "ECO:0001810": {
        "comment": [
            "In DNAse footingprinting amplified DNA combined with TF and DNAse is electrophoresed for fragment comparison with a non-TF control sample. TF-bound fragments do not appear in the gel. Such fragments can be isolated, purified, and sequenced. This technique is often used to identify the binding motif of a TF resolving the protected region to 50-100 bp which can be further examined for TF-binding sites."
        ],
        "created_by": [
            "swolfish"
        ],
        "creation_date": [
            "2015-11-03T13:55:15Z"
        ],
        "def": [
            "\"A type of nucleic acid binding evidence where proteins that have been bound to DNA protect a binding site from enzymatic cleavage with DNAse, thereby detecting protein-DNA interactions.\" [ECO:SW, PMID:212715, PMID:22194258]"
        ],
        "id": [
            "ECO:0001810"
        ],
        "is_a": [
            "ECO:0000136 ! nucleic acid binding evidence"
        ],
        "name": [
            "DNAse footprinting evidence"
        ],
        "synonym": [
            "\"DNase protection\" RELATED []"
        ]
    },
    "ECO:0001811": {
        "comment": [
            "The degree of polarization of a fluorophore is inversely related to its molecular rotation. When the fluorophore is bound to a small molecule, the rate at which it tumbles can decrease significantly from when it is bound tightly to a large protein. If the fluorophore is attached to the larger protein in a binding pair, the difference in polarization between bound and unbound states will be smaller and less accurate. The degree of binding is calculated by using the difference in anisotropy of the partially bound, free, and fully bound states measured by titrating the two binding partners. Fluorescence polarixation assays are homogeneous."
        ],
        "created_by": [
            "swolfish"
        ],
        "creation_date": [
            "2015-11-03T14:01:36Z"
        ],
        "def": [
            "\"A type of fluorescence evidence based on rapid and quantitative analysis of diverse molecular interactions, enzyme activities, and nucleic acid hybridization which uses a fluorophore to measure the binding constants and kinetics of reactions that cause a change in the rotational time of the molecules.\" [ECO:SW, PMID:20232898]"
        ],
        "id": [
            "ECO:0001811"
        ],
        "is_a": [
            "ECO:0001115 ! fluorescence evidence"
        ],
        "name": [
            "fluorescence anisotropy evidence"
        ],
        "synonym": [
            "\"FA\" EXACT []",
            "\"Fluorescence polarization\" RELATED [PMCID:3277431, PMID:20232898]",
            "\"FP\" RELATED [PMCID:3277431, PMID:20232898]"
        ]
    },
    "ECO:0001812": {
        "comment": [
            "The reported sequences should always be verified for presence in the genome sequence."
        ],
        "created_by": [
            "swolfish"
        ],
        "creation_date": [
            "2015-11-03T14:05:37Z"
        ],
        "def": [
            "\"A type of systematic evolution of ligands by exponential amplification evidence based on a restricted genomic DNA library to identify naturally occurring genomic aptamers and RNA-protein interaction networks with RNA-binding protein as bait and high-throughput sequencing.\" [ECO:SW, PMID:20541015, PMID:21720957]"
        ],
        "id": [
            "ECO:0001812"
        ],
        "is_a": [
            "ECO:0000293 ! systematic evolution of ligands by exponential amplification evidence"
        ],
        "name": [
            "genomic systematic evolution of ligands by exponential amplification evidence"
        ],
        "synonym": [
            "\"genomic SELEX\" EXACT [PMID:20541015]"
        ]
    },
    "ECO:0001813": {
        "comment": [
            "Chemical shifts in the spectrum indicating binding can be detected from analysis of a protein spectrum in the presence or absence of its cognate DNA binding site."
        ],
        "created_by": [
            "swolfish"
        ],
        "creation_date": [
            "2015-11-03T14:11:06Z"
        ],
        "def": [
            "\"A type of nuclear magnetic resonance spectroscopy evidence based on two-dimensional NMR for elucidation of the chemical structure of an isolated or synthesized chemical compound where the characteristic transfer magnetization of a proton to a nitrogen or carbon isotope is monitored by NMR, generating a specific peak in the spectrum.\" [ECO:SW, PMID:19856946]"
        ],
        "id": [
            "ECO:0001813"
        ],
        "is_a": [
            "ECO:0001105 ! nuclear magnetic resonance spectroscopy evidence"
        ],
        "name": [
            "heteronuclear single quantum coherence spectroscopy evidence"
        ],
        "synonym": [
            "\"HSQC\" EXACT [PMID:19856946]"
        ]
    },
    "ECO:0001814": {
        "created_by": [
            "swolfish"
        ],
        "creation_date": [
            "2015-11-03T14:24:04Z"
        ],
        "def": [
            "\"A type of nucleic acid binding evidence where the synthetic molecule methidiumpropyl-EDTA (MPE) is used to cleave ligand-protected DNA, followed by analysis of the restriction fragments to generate a footprint (i.e. size and location) of small molecule binding sites on the DNA.\" [ECO:SW, PMID:6225070]"
        ],
        "id": [
            "ECO:0001814"
        ],
        "is_a": [
            "ECO:0000136 ! nucleic acid binding evidence"
        ],
        "name": [
            "methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence"
        ],
        "synonym": [
            "\"Methidiumpropyl-EDTA Fe(II) footprinting\" RELATED [PMID:6225070]",
            "\"MPE Fe(II) footprinting\" RELATED [PMID:6225070]",
            "\"MPE-EDTA Fe(II) footprinting\" RELATED [PMID:6225070]"
        ]
    },
    "ECO:0001815": {
        "created_by": [
            "swolfish"
        ],
        "creation_date": [
            "2015-11-04T08:37:51Z"
        ],
        "def": [
            "\"A type of nucleic acid binding evidence where nucleic acid that has been bound to protein is cleaved with 1,10-phenanthroline-copper complex resulting in a high-resolution footprint of sequence-specific protein-DNA contacts.\" [ECO:SW, PMID:11691942]"
        ],
        "id": [
            "ECO:0001815"
        ],
        "is_a": [
            "ECO:0000136 ! nucleic acid binding evidence"
        ],
        "name": [
            "copper-phenanthroline footprinting evidence"
        ],
        "synonym": [
            "\"1,10-Phenanthroline-copper footprinting\" EXACT [PMID:1384472]",
            "\"OP-Cu Complex\" RELATED [PMID:1384472]"
        ]
    },
    "ECO:0001816": {
        "created_by": [
            "swolfish"
        ],
        "creation_date": [
            "2015-11-04T08:58:42Z"
        ],
        "def": [
            "\"A type of reporter gene assay evidence based on the fusion of select genes with the green fluorescent protein (GFP) gene for detection with bioluminescence of the gene product when exposed to blue ultraviolet light.\" [ECO:SW, PMID:11989662]"
        ],
        "id": [
            "ECO:0001816"
        ],
        "is_a": [
            "ECO:0000049 ! reporter gene assay evidence"
        ],
        "name": [
            "green fluorescent protein reporter gene assay evidence"
        ],
        "synonym": [
            "\"GFP promoter fusion\" RELATED []",
            "\"GFP reporter gene assay\" EXACT [PMID:11989662]",
            "\"green fluorescent protein promoter fusion\" RELATED []"
        ]
    },
    "ECO:0001817": {
        "comment": [
            "The recombinant protein can also be eluted following the pull-down and analyzed further with qPCR or sequencing."
        ],
        "created_by": [
            "swolfish"
        ],
        "creation_date": [
            "2015-11-04T09:05:56Z"
        ],
        "def": [
            "\"A type of protein binding evidence where a gene is fused with the GST gene and the resulting recombinant bait protein is captured on an immobilized Glutathione affinity ligand and incubated with prey protein to identify and characterize protein-protein interactions.\" [ECO:SW, PMID:26096507]"
        ],
        "id": [
            "ECO:0001817"
        ],
        "is_a": [
            "ECO:0000024 ! protein binding evidence"
        ],
        "name": [
            "glutathione S-transferase pull-down assay evidence"
        ],
        "synonym": [
            "\"GST pull-down assay\" EXACT [PMID:26096507]"
        ]
    },
    "ECO:0001818": {
        "created_by": [
            "swolfish"
        ],
        "creation_date": [
            "2015-11-04T09:20:26Z"
        ],
        "def": [
            "\"A type of nucleic acid binding evidence used to identify protein-binding sites on the DNA molecule where DNA that has been bound to protein is digested with hydroxyl radical produced by reduction of hydrogen peroxide with iron (II), followed by separating the cleavage products on a denaturing electrophoresis gel.\" [ECO:SW, PMID:18546600, PMID:19378159, PMID:3090544]"
        ],
        "id": [
            "ECO:0001818"
        ],
        "is_a": [
            "ECO:0000136 ! nucleic acid binding evidence"
        ],
        "name": [
            "hydroxyl-radical footprinting evidence"
        ]
    },
    "ECO:0001819": {
        "comment": [
            "The most commonly used radiolabel is 32P."
        ],
        "created_by": [
            "swolfish"
        ],
        "creation_date": [
            "2015-11-04T09:33:18Z"
        ],
        "def": [
            "\"A type of transcript expression level evidence used to determine expression levels of mRNA where a radiolabeled synthetic oligonucleotide primer is annealed to mRNA at the transcription start site of a gene and the mRNA is extended with reverse transcriptase producing cDNA which is electrophoresed on a polyacrylamide gel and autoradiographed, and the band size is proportional to the initial RNA.\" [ECO:SW, PMID:23378648]"
        ],
        "id": [
            "ECO:0001819"
        ],
        "is_a": [
            "ECO:0000048 ! transcript expression level evidence"
        ],
        "name": [
            "primer extension assay evidence"
        ]
    },
    "ECO:0001820": {
        "comment": [
            "RACE PCR is frequently used to verify transcription start sites relevant to the function of transcription factor binding sites such as repression."
        ],
        "created_by": [
            "swolfish"
        ],
        "creation_date": [
            "2015-11-04T09:48:38Z"
        ],
        "def": [
            "\"A type of polymerase chain reaction evidence where a mRNA primer is used for RT-PCR and terminal deoxynucleotidyl transferase (TdT) is used to add identical nucleotides to the 3' end of the cDNA after which PCR with a different primer and TdT-oligomer are performed in the other sense to obtain the full length sequence of a RNA transcript.\" [ECO:SW, PMID:17498297]"
        ],
        "id": [
            "ECO:0001820"
        ],
        "is_a": [
            "ECO:0000082 ! polymerase chain reaction evidence"
        ],
        "name": [
            "rapid amplification of cDNA ends polymerase chain reaction evidence"
        ],
        "synonym": [
            "\"RACE PCR\" EXACT [PMI:17498297]"
        ]
    },
    "ECO:0001821": {
        "created_by": [
            "swolfish"
        ],
        "creation_date": [
            "2015-11-04T10:15:57Z"
        ],
        "def": [
            "\"A type of nucleotide sequencing assay evidence where RNA is extracted from a cell, reverse transcribed to cDNA for a snapshot of an organism's transcriptome with precise measurement of transcript levels and their isoforms.\" [ECO:SW, PMID:19015660]"
        ],
        "id": [
            "ECO:0001821"
        ],
        "is_a": [
            "ECO:0000219 ! nucleotide sequencing assay evidence"
        ],
        "name": [
            "RNA sequencing assay evidence"
        ],
        "synonym": [
            "\"RNA-Seq\" EXACT [PMID:23851085]",
            "\"Whole Transcriptome Shotgun Sequencing\" EXACT [PMID:23851085]",
            "\"WTTS\" EXACT []"
        ]
    },
    "ECO:0001822": {
        "created_by": [
            "swolfish"
        ],
        "creation_date": [
            "2015-11-04T10:48:59Z"
        ],
        "def": [
            "\"A type of knockout evidence based on the survival of an organism in a particular environment where a gene for an enzyme or regulator is knocked out and results are used as a natural reporter.\" [ECO:SW]"
        ],
        "id": [
            "ECO:0001822"
        ],
        "is_a": [
            "ECO:0001091 ! knockout evidence"
        ],
        "name": [
            "survival analysis evidence"
        ]
    },
    "ECO:0001823": {
        "comment": [
            "X-ray crystallography can be used for a crystal used to probe at the tridimensional structure of proteins. In some cases, it is possible to co-crystallize the protein bound to its DNA binding site, providing detail on the particular arrangement of the two components."
        ],
        "created_by": [
            "swolfish"
        ],
        "creation_date": [
            "2015-11-04T10:56:22Z"
        ],
        "def": [
            "\"A type of crystallography evidence where a purified sample at high concentration is crystallised and the crystals are exposed to an x ray beam to obtain three dimensional molecular structure of proteins and biological macromolecules.\" [ECO:SW, PMID:23135450, PMID:24648090]"
        ],
        "id": [
            "ECO:0001823"
        ],
        "is_a": [
            "ECO:0001034 ! crystallography evidence"
        ],
        "name": [
            "x-ray crystallography evidence"
        ]
    },
    "ECO:0001824": {
        "comment": [
            "The methylation tag is detected with methylation-sensitive restriction enzymes, DpnI and DpnII."
        ],
        "created_by": [
            "swolfish"
        ],
        "creation_date": [
            "2015-11-05T15:56:13Z"
        ],
        "def": [
            "\"A type of affinity evidence used to identify DNA binding sites in eukaryotes where Escherichia coli DNA adenine methyltransferase (Dam) is fused to a transcription factor, co-factor, chromatin-associated protein, or nuclear-associated protein, followed by a methyl-dependent PCR to localize methyltransferase in the region of the binding site.\" [ECO:SW, PMID:16938559, PMID:17545983, PMID:19588092]"
        ],
        "id": [
            "ECO:0001824"
        ],
        "is_a": [
            "ECO:0000023 ! affinity evidence"
        ],
        "name": [
            "DNA adenine methyltransferase identification evidence"
        ],
        "synonym": [
            "\"DamID\" EXACT [PMID:19588092]"
        ]
    },
    "ECO:0001825": {
        "comment": [
            "When ITC is used to study TF, the temperature of two identical cells containing a known concentration of TF is monitored. After the ligand is added to the cells in precisely measured aliquots, the temperature difference in each cell is observed to determine target binding. This value can be used to compute the energetics of the reaction and hence, binding affinity of the TF for the DNA fragment. The thermodynamic profile that is measured includes the values of binding constant (K(a)), stoichiometry (n), and the enthalpy of binding (DeltaH(b))."
        ],
        "created_by": [
            "swolfish"
        ],
        "creation_date": [
            "2015-11-05T16:06:20Z"
        ],
        "def": [
            "\"A type of affinity evidence where the absorbed or released heat of a biomolecular binding event is directly measured in a reference cell and sample cell after the addition of a ligand using a microcalorimeter for a complete thermodynamic profile of the molecular interaction.\" [ECO:SW, PMID:10527727]"
        ],
        "id": [
            "ECO:0001825"
        ],
        "is_a": [
            "ECO:0000023 ! affinity evidence"
        ],
        "name": [
            "isothermal titration calorimetry evidence"
        ],
        "synonym": [
            "\"ITC\" EXACT [PMID:10527727]"
        ]
    },
    "ECO:0001826": {
        "created_by": [
            "swolfish"
        ],
        "creation_date": [
            "2015-11-05T16:13:36Z"
        ],
        "def": [
            "\"A type of nucleic acid binding evidence where DNA is non-specifically fragmented with ultraviolet light while protein-bound regions are protected from UV damage and strand breakage patterns are analyzed by PAGE and sequenced to detect protein-DNA contacts.\" [ECO:SW, PMID:2842760, PMID:7602584]"
        ],
        "id": [
            "ECO:0001826"
        ],
        "is_a": [
            "ECO:0000136 ! nucleic acid binding evidence"
        ],
        "name": [
            "ultraviolet light footprinting evidence"
        ],
        "synonym": [
            "\"ultraviolet footprinting \" RELATED []",
            "\"UV footprinting\" RELATED []"
        ]
    },
    "ECO:0001827": {
        "created_by": [
            "swolfish"
        ],
        "creation_date": [
            "2015-11-05T16:16:27Z"
        ],
        "def": [
            "\"A type of nucleic acid binding evidence used to identify protein binding sites on the DNA molecule where multiple copies of a DNA fragment containing a putative TF-binding site are randomly methylated with dimethyl sulfate (DMS) and cleaved at the methyl group, followed by separating the cleavage products on a denaturing electrophoresis gel.\" [ECO:SW, PMID:1583685]"
        ],
        "id": [
            "ECO:0001827"
        ],
        "is_a": [
            "ECO:0000136 ! nucleic acid binding evidence"
        ],
        "name": [
            "premethylation interference footprinting evidence"
        ],
        "synonym": [
            "\"methylation interference footprinting\" RELATED []"
        ]
    },
    "ECO:0001828": {
        "created_by": [
            "swolfish"
        ],
        "creation_date": [
            "2015-11-10T14:40:16Z"
        ],
        "def": [
            "\"A type of inference from background scientific knowledge used in manual assertion where a visual inspection of promoter region is made to identify putative binding sites.\" [ECO:SW]"
        ],
        "id": [
            "ECO:0001828"
        ],
        "is_a": [
            "ECO:0000306 ! inference from background scientific knowledge used in manual assertion"
        ],
        "name": [
            "visual sequence inspection evidence"
        ]
    },
    "ECO:0001829": {
        "created_by": [
            "swolfish"
        ],
        "creation_date": [
            "2015-11-10T14:55:36Z"
        ],
        "def": [
            "\"A type of reporter gene assay evidence used to locate and isolate Fur sites where a plasmid library is created and cloned into a cell line with a ferric uptake regulator (Fur)-repressed reporter and the Fur protein is titrated away from its binding site on the reporter, after which the cell is isolated and plasmid extracted for further sequence analysis.\" [ECO:SW, PMID:10713425, PMID:7642488, PMID:8107138]"
        ],
        "id": [
            "ECO:0001829"
        ],
        "is_a": [
            "ECO:0000049 ! reporter gene assay evidence"
        ],
        "name": [
            "ferric uptake regulator titration assay evidence"
        ],
        "synonym": [
            "\"Fluorescence polarization\" RELATED [PMID:15689115]",
            "\"FURTA\" EXACT [PMID:10713425]"
        ]
    },
    "ECO:0005004": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-02-13T12:08:01Z"
        ],
        "id": [
            "ECO:0005004"
        ],
        "is_a": [
            "ECO:0005014 ! in vitro cell based assay evidence"
        ],
        "name": [
            "cell viability assay evidence"
        ]
    },
    "ECO:0005007": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-02-11T16:27:13Z"
        ],
        "id": [
            "ECO:0005007"
        ],
        "is_a": [
            "ECO:0005014 ! in vitro cell based assay evidence"
        ],
        "name": [
            "cell proliferation assay evidence"
        ]
    },
    "ECO:0005008": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-02-11T16:30:10Z"
        ],
        "id": [
            "ECO:0005008"
        ],
        "is_a": [
            "ECO:0005007 ! cell proliferation assay evidence"
        ],
        "name": [
            "DNA synthesis cell proliferation assay evidence"
        ]
    },
    "ECO:0005009": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-02-11T16:36:33Z"
        ],
        "id": [
            "ECO:0005009"
        ],
        "is_a": [
            "ECO:0005007 ! cell proliferation assay evidence"
        ],
        "name": [
            "metabolic cell proliferation assay evidence"
        ]
    },
    "ECO:0005010": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-02-11T16:37:23Z"
        ],
        "id": [
            "ECO:0005010"
        ],
        "is_a": [
            "ECO:0005007 ! cell proliferation assay evidence"
        ],
        "name": [
            "cell proliferation marker detection assay evidence"
        ]
    },
    "ECO:0005011": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-02-11T16:47:30Z"
        ],
        "id": [
            "ECO:0005011"
        ],
        "is_a": [
            "ECO:0005007 ! cell proliferation assay evidence"
        ],
        "name": [
            "ATP bioluminescence assay evidence"
        ]
    },
    "ECO:0005012": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-02-12T11:39:35Z"
        ],
        "id": [
            "ECO:0005012"
        ],
        "is_a": [
            "ECO:0005014 ! in vitro cell based assay evidence"
        ],
        "name": [
            "cytotoxicity assay evidence"
        ]
    },
    "ECO:0005014": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-02-12T13:26:55Z"
        ],
        "id": [
            "ECO:0005014"
        ],
        "is_a": [
            "ECO:0000181 ! in vitro assay evidence"
        ],
        "name": [
            "in vitro cell based assay evidence"
        ]
    },
    "ECO:0005019": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-02-19T11:48:57Z"
        ],
        "id": [
            "ECO:0005019"
        ],
        "is_a": [
            "ECO:0000002 ! direct assay evidence"
        ],
        "name": [
            "staining evidence"
        ]
    },
    "ECO:0005021": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-02-19T13:10:20Z"
        ],
        "id": [
            "ECO:0005021"
        ],
        "is_a": [
            "ECO:0005014 ! in vitro cell based assay evidence"
        ],
        "name": [
            "chemotaxis assay evidence"
        ]
    },
    "ECO:0005027": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-03-16T15:33:50Z"
        ],
        "id": [
            "ECO:0005027"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "genetic transformation evidence"
        ]
    },
    "ECO:0005031": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-03-20T12:16:17Z"
        ],
        "id": [
            "ECO:0005031"
        ],
        "is_a": [
            "ECO:0000006 ! experimental evidence"
        ],
        "name": [
            "structure determination evidence"
        ]
    },
    "ECO:0005033": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-03-20T12:21:48Z"
        ],
        "id": [
            "ECO:0005033"
        ],
        "is_a": [
            "ECO:0001098 ! microscopy evidence",
            "ECO:0005031 ! structure determination evidence"
        ],
        "name": [
            "electron microscopy evidence"
        ]
    },
    "ECO:0005034": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-04-07T21:58:24Z"
        ],
        "id": [
            "ECO:0005034"
        ],
        "is_a": [
            "ECO:0005014 ! in vitro cell based assay evidence"
        ],
        "name": [
            "apoptotic assay evidence"
        ]
    },
    "ECO:0005500": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-06-15T15:35:05Z"
        ],
        "def": [
            "\"A type of in vivo assay evidence where ADP-ribosylated residues in proteins are detected in cells in vivo.\" [ECO:SN, PMID:2820766, SIB:PG]"
        ],
        "id": [
            "ECO:0005500"
        ],
        "is_a": [
            "ECO:0000178 ! in vivo assay evidence"
        ],
        "name": [
            "in vivo polyADP-ribosylation assay evidence"
        ]
    },
    "ECO:0005501": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-06-15T15:37:23Z"
        ],
        "def": [
            "\"A type of in vivo polyADP-ribosylation assay evidence that is used in a manual assertion.\" [ECO:SN]"
        ],
        "id": [
            "ECO:0005501"
        ],
        "intersection_of": [
            "ECO:0005500 ! in vivo polyADP-ribosylation assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion",
            "ECO:0005500 ! in vivo polyADP-ribosylation assay evidence"
        ],
        "name": [
            "in vivo polyADP-ribosylation assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0005502": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-06-26T11:46:15Z"
        ],
        "def": [
            "\"A type of biological assay evidence derived by studying an organ, tissues, or cells taken from an organism in an external environment with the minimum alteration of natural conditions.\" [ECO:SN, PMID:16305312, url:http\\://www.ncbi.nlm.nih.gov/pmc/articles/PMC2305722/pdf/nihms42807.pdf]"
        ],
        "id": [
            "ECO:0005502"
        ],
        "is_a": [
            "ECO:0000094 ! biological assay evidence"
        ],
        "name": [
            "ex vivo assay evidence"
        ]
    },
    "ECO:0005503": {
        "comment": [
            "Two-dimensional polyacrylamide gel electrophoresis is commonly employed to establish a proteome for an organism and demonstrate expression changes."
        ],
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-10-01T16:23:20Z"
        ],
        "def": [
            "\"A type of gel electrophoresis evidence that involves one dimension electrophoresis where biomolecules are electrophoresed on a low percentage polyacrylamide gel  to be separated in proportion to their mass or isoelectric point, followed by high voltage electrophoresis on a higher percentage gel in the presence of ethidium bromide to alter non-linear molecular shape.\" [ECO:SW, url:http\\://www.nature.com/nmeth/journal/v2/n1/full/nmeth0105-83.html]"
        ],
        "id": [
            "ECO:0005503"
        ],
        "is_a": [
            "ECO:0000337 ! gel electrophoresis evidence"
        ],
        "name": [
            "two-dimensional polyacrylamide gel electrophoresis evidence"
        ],
        "synonym": [
            "\"2D PAGE\" EXACT []",
            "\"2D-PAGE\" EXACT []"
        ]
    },
    "ECO:0005504": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-08-14T15:48:08Z"
        ],
        "id": [
            "ECO:0005504"
        ],
        "is_a": [
            "ECO:0000006 ! experimental evidence"
        ],
        "name": [
            "spectrometry evidence"
        ]
    },
    "ECO:0005505": {
        "comment": [
            "Regular expression is a computational tool used for determining whether a given genome contains a DNA region resembling a transcription factor binding motif. For example A/T=W indicates A or T may be present in a column of the alignment. (PMID: 20708667)."
        ],
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-10-09T13:51:52Z"
        ],
        "def": [
            "\"A type of motif similarity evidence where the motif, a local alignment of sequences, is represented by a regular expression, which captures the variability of nucleotides at each position in a discrete form and can then be used to search genetic sequences by identifying matches to the regular expression.\" [ECO:SW, PMID:17337630]"
        ],
        "id": [
            "ECO:0005505"
        ],
        "is_a": [
            "ECO:0000028 ! motif similarity evidence"
        ],
        "name": [
            "regular expression motif search evidence"
        ]
    },
    "ECO:0005506": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-09-18T16:52:34Z"
        ],
        "id": [
            "ECO:0005506"
        ],
        "is_a": [
            "ECO:0001113 ! point mutation evidence"
        ],
        "name": [
            "missense mutation evidence"
        ]
    },
    "ECO:0005507": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-09-18T16:55:32Z"
        ],
        "id": [
            "ECO:0005507"
        ],
        "is_a": [
            "ECO:0001113 ! point mutation evidence"
        ],
        "name": [
            "nonsense mutation evidence"
        ]
    },
    "ECO:0005508": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-09-18T17:01:12Z"
        ],
        "id": [
            "ECO:0005508"
        ],
        "is_a": [
            "ECO:0001113 ! point mutation evidence"
        ],
        "name": [
            "silent mutation evidence"
        ]
    },
    "ECO:0005509": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-09-18T17:02:03Z"
        ],
        "id": [
            "ECO:0005509"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "insertion mutation evidence"
        ]
    },
    "ECO:0005510": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-09-18T17:02:45Z"
        ],
        "id": [
            "ECO:0005510"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "deletion mutation evidence"
        ]
    },
    "ECO:0005511": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-09-18T17:03:25Z"
        ],
        "id": [
            "ECO:0005511"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "duplication mutation evidence"
        ]
    },
    "ECO:0005512": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-09-18T17:05:49Z"
        ],
        "id": [
            "ECO:0005512"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "frameshift mutation evidence"
        ]
    },
    "ECO:0005513": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-09-18T17:06:34Z"
        ],
        "id": [
            "ECO:0005513"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "repeat expansion mutation evidence"
        ]
    },
    "ECO:0005514": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-09-18T17:07:29Z"
        ],
        "id": [
            "ECO:0005514"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "splice site mutation evidence"
        ]
    },
    "ECO:0005515": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-09-18T17:08:01Z"
        ],
        "id": [
            "ECO:0005515"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "translocation mutation evidence"
        ]
    },
    "ECO:0005516": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-09-24T15:54:21Z"
        ],
        "id": [
            "ECO:0005516"
        ],
        "is_a": [
            "ECO:0000006 ! experimental evidence"
        ],
        "name": [
            "molecule detection assay evidence"
        ]
    },
    "ECO:0005517": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-09-25T14:27:40Z"
        ],
        "id": [
            "ECO:0005517"
        ],
        "is_a": [
            "ECO:0005516 ! molecule detection assay evidence"
        ],
        "name": [
            "protein detection assay evidence"
        ]
    },
    "ECO:0005518": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-09-25T14:43:53Z"
        ],
        "id": [
            "ECO:0005518"
        ],
        "is_a": [
            "ECO:0005516 ! molecule detection assay evidence"
        ],
        "name": [
            "RNA detection assay evidence"
        ]
    },
    "ECO:0005519": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-09-25T19:33:25Z"
        ],
        "id": [
            "ECO:0005519"
        ],
        "is_a": [
            "ECO:0005516 ! molecule detection assay evidence"
        ],
        "name": [
            "DNA detection assay evidence"
        ]
    },
    "ECO:0005520": {
        "comment": [
            "Differences in the optical path lengths between a surface layer and a buried silica layer can be measured very precisely allowing optical height information to be converted to accumulated mass on the surface. For the purpose of TF-binding site identification, IRIS is coupled with DNA-arrays with immobilized potential binding regions exposed to the protein of interest and eluted. Protein-bound fragments exhibit changes in reflectance that can be used to gauge binding quantitatively."
        ],
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-09-28T16:01:01Z"
        ],
        "def": [
            "\"A type of molecule detection assay evidence based on changes in surface-reflected light due to ligand binding on the surface of a semi-transparent substrate to detect proteins, DNA, and other biological material in a multiplexed, high-throughput microarray format without labels.\" [ECO:SW, PMID:21587155, PMID:24271115]"
        ],
        "id": [
            "ECO:0005520"
        ],
        "is_a": [
            "ECO:0005516 ! molecule detection assay evidence"
        ],
        "name": [
            "interferometric reflectance imaging sensor evidence"
        ],
        "synonym": [
            "\"IRIS\" EXACT []",
            "\"Spectral Reflectance Imaging Biosensor\" EXACT []",
            "\"SRIB\" EXACT []"
        ]
    },
    "ECO:0005521": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-09-28T17:16:56Z"
        ],
        "def": [
            "\"A type of nuclease protection assay evidence where RNA is hybridized with complementary DNA probes and exposed to S1 nuclease for unbound RNA degradation after which the intact RNA is run on a gel for probe size determination and RNA identification to detect and map specific RNAs in a complex mixture of total cellular RNA.\" [ECO:SW, PMID:21390683]"
        ],
        "id": [
            "ECO:0005521"
        ],
        "is_a": [
            "ECO:0001106 ! nuclease protection assay evidence"
        ],
        "name": [
            "S1 nuclease protection assay evidence"
        ]
    },
    "ECO:0005522": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-09-30T10:03:03Z"
        ],
        "id": [
            "ECO:0005522"
        ],
        "is_a": [
            "ECO:0005519 ! DNA detection assay evidence"
        ],
        "name": [
            "DNA dot blot assay evidence"
        ]
    },
    "ECO:0005523": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-09-30T10:04:00Z"
        ],
        "def": [
            "\"A type of DNA dot blot assay evidence that is used in a manual assertion.\" [ECO:SN, ECO:SW]"
        ],
        "id": [
            "ECO:0005523"
        ],
        "intersection_of": [
            "ECO:0005522 ! DNA dot blot assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0005522 ! DNA dot blot assay evidence"
        ],
        "name": [
            "DNA dot blot assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0005524": {
        "comment": [
            "The mRNA extraction is performed in induced and non-induced cells and reverse transcribed with different fluorophore labeling for controls and experiment. Fluorescence is measured at two different wavelengths and the ratio between the two fluorescence intensities corresponds to the induction level."
        ],
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-09-30T10:12:48Z"
        ],
        "def": [
            "\"A type of DNA detection assay evidence where complimentary ssDNA is attached to a solid glass, silicon, or plastic surface and is hybridized with a labeled sample of extracted mRNA or amplified cDNA and stimulated with a laser for fluorescence detected with a scanner.\" [ECO:SW, PMID:17660860]"
        ],
        "id": [
            "ECO:0005524"
        ],
        "is_a": [
            "ECO:0005519 ! DNA detection assay evidence"
        ],
        "name": [
            "DNA microarray evidence"
        ],
        "synonym": [
            "\"DNA chip\" RELATED [PMID:10398077]",
            "\"DNA-array expression analysis \" RELATED []"
        ]
    },
    "ECO:0005525": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-09-30T10:13:09Z"
        ],
        "def": [
            "\"A type of DNA microarray evidence that is used in a manual assertion.\" [ECO:SN, ECO:SW]"
        ],
        "id": [
            "ECO:0005525"
        ],
        "intersection_of": [
            "ECO:0005524 ! DNA microarray evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0005524 ! DNA microarray evidence"
        ],
        "name": [
            "DNA microarray evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0005526": {
        "comment": [
            "MALDI-TOF MS is commonly used for protein identification in constructing a proteome where protein fingerprints obtained by microbial cells are compared with a database of reference spectra by means of various algorithms integrated in systems recently made commercially available."
        ],
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-09-30T10:51:56Z"
        ],
        "def": [
            "\"A type of protein detection assay evidence where soft ionization is used for the analysis of biomolecules for mass determination in a three step process of plate preparation, radiation, and ionization with high throughput technology.\" [ECO:SW, PMID:21964792, PMID:25354905]"
        ],
        "id": [
            "ECO:0005526"
        ],
        "is_a": [
            "ECO:0005517 ! protein detection assay evidence"
        ],
        "name": [
            "matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence"
        ],
        "synonym": [
            "\"MALDI-TOF Mass Spectrometry\" EXACT []",
            "\"MALDI-TOF-MS\" EXACT []"
        ]
    },
    "ECO:0005527": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-09-30T10:58:51Z"
        ],
        "def": [
            "\"A type of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence that is  used in a manual assertion.\" [ECO:SN, ECO:SW]"
        ],
        "id": [
            "ECO:0005527"
        ],
        "intersection_of": [
            "ECO:0005526 ! matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0005526 ! matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence"
        ],
        "name": [
            "matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0005528": {
        "comment": [
            "Site-directed mutagenesis is often performed in tandem with EMSA. It is often implemented only in conserved motif positions or serially through all positions of a site."
        ],
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-09-30T11:34:41Z"
        ],
        "def": [
            "\"A type of experimental phenotypic evidence based on target-specified mutagenesis for the characterization of various gene and protein interactions, structures, and functions where oligonucleotide primers are used to alter a nucleotide sequence.\" [ECO:SW, PMID:21204030, PMID:24011050, PMID:3541892]"
        ],
        "id": [
            "ECO:0005528"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "site-directed mutagenesis evidence"
        ],
        "synonym": [
            "\"site directed mutagenesis\" EXACT []"
        ]
    },
    "ECO:0005529": {
        "comment": [
            "Random mutagenesis is not often used in TF-binding site determination; however, it is sometimes used to investigate the effect of mutations in TF binding domains in conjunction with expression assays. Random mutations can also be introduced via chemicals, error-prone PCR (EP-PCR), degenerate oligonucleotides-Pfu(DOP), UV irradiation, mutator strains, nucleotide analogs, or DNA recombination."
        ],
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-09-30T11:55:03Z"
        ],
        "def": [
            "\"A type of experimental phenotypic evidence where radiation or a DNA-damaging agent in vivo, or PCR with degenerate primers in vitro are used for non-targeted mutagenesis.\" [ECO:SW, PMID:15153637, PMID:18265275]"
        ],
        "id": [
            "ECO:0005529"
        ],
        "is_a": [
            "ECO:0000059 ! experimental phenotypic evidence"
        ],
        "name": [
            "random mutagenesis evidence"
        ]
    },
    "ECO:0005530": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-09-30T11:57:00Z"
        ],
        "def": [
            "\"A type of random mutagenesis evidence that is used in a manual assertion.\" []"
        ],
        "id": [
            "ECO:0005530"
        ],
        "intersection_of": [
            "ECO:0005529 ! random mutagenesis evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion",
            "ECO:0005529 ! random mutagenesis evidence"
        ],
        "name": [
            "random mutagenesis evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0005531": {
        "comment": [
            "Relationship type between sequences (e.g. orthologs from multiple genomes or co-expressed genes in the same genome) is not necessarily assumed. The local multiple sequence alignment is typically ungapped. A common application of motif discovery is to infer the binding motif of a given transcription factor (TF). This can be based on the results of a whole-genome expression analysis, assuming that co-expressed genes are co-regulated by the same TF (PMID: 19892760), from peaks in ChIP-seq experiments with the TF (PMID: 16264194), or from the comparative genomics analysis of the promoter regions of orthologs (PMID: 23032607)."
        ],
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-09-30T15:04:45Z"
        ],
        "def": [
            "\"A type of multiple sequence alignment evidence based on a set of algorithms that infer over-represented motifs from a set of biological sequences returning one or more local multiple sequence alignment defining the inferred motifs.\" [ECO:SW, PMID:10812473, PMID:16845028, PMID:8211139]"
        ],
        "id": [
            "ECO:0005531"
        ],
        "is_a": [
            "ECO:0005555 ! multiple sequence alignment evidence"
        ],
        "name": [
            "motif discovery evidence"
        ]
    },
    "ECO:0005532": {
        "comment": [
            "This technique is used to identify transcription factor binding sites by searching a bacterial genome for DNA regions resembling a given TF-binding motif. PMID: 17085555."
        ],
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-10-09T14:12:54Z"
        ],
        "def": [
            "\"A type of motif similarity evidence where the motif is represented by a consensus sequence which incorporates the most frequent nucleotide at each position acting as a generic representative for the sequences in the alignment, and is compared to the genetic sequence  for a perfect or imperfect match.\" [ECO:SW, PMID:15130839]"
        ],
        "id": [
            "ECO:0005532"
        ],
        "is_a": [
            "ECO:0000028 ! motif similarity evidence"
        ],
        "name": [
            "consensus search evidence"
        ]
    },
    "ECO:0005533": {
        "comment": [
            "The name derives to experimental footprinting techniques where transcription factor (TF)-binding sites are identified by sequencing the region of DNA protected from biochemical agents by the bound TF. This technique is used to identify or verify transcription factor-binding motifs, most commonly in the Eukaryota (PMID: 11997340)."
        ],
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-09-30T16:23:02Z"
        ],
        "def": [
            "\"A type of motif discovery evidence based on a set of algorithms that infer motifs in non-coding DNA sequences from the conservation of functional regulatory sites through evolution at a higher rate than their local surroundings for the identification of an evolutionary 'footprint'.\" [ECO:SW, PMID:16477324, PMID:3199442]"
        ],
        "id": [
            "ECO:0005533"
        ],
        "is_a": [
            "ECO:0005531 ! motif discovery evidence"
        ],
        "name": [
            "phylogenetic footprinting evidence"
        ]
    },
    "ECO:0005534": {
        "comment": [
            "This technique is used to predict transcription factor binding sites in gene promoter regions by identifying conserved DNA regions across multiple genomes. PMID: 22305460. Orthology is a typical comparative criterion used."
        ],
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-10-09T14:30:38Z"
        ],
        "def": [
            "\"A type of motif similarity evidence based on comparative genomics which uses a search method to scan a set of genomes for instances of a specific motif, and then applies a comparative criterion to analyze the collective strength of these predictions, based on the assumption that functional instances of the motif will be preserved by natural selection and their prediction will therefore be consistent across genomes.\" [ECO:SW, PMID:10854408]"
        ],
        "id": [
            "ECO:0005534"
        ],
        "is_a": [
            "ECO:0000028 ! motif similarity evidence"
        ],
        "name": [
            "comparative genomics motif search evidence"
        ]
    },
    "ECO:0005535": {
        "comment": [
            "Machine learning algorithms include, but are not limited to artificial neural networks, random-forest, k-means clustering, hidden Markov models. This suite of methods can be used to identify or verify transcription factor-binding sites in genomic sequences (PMID: 15703297)."
        ],
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-10-01T11:58:36Z"
        ],
        "def": [
            "\"A type of match to sequence model evidence which captures the application of a broad suite of supervised and unsupervised machine learning algorithms trained on known motif instances and applied to the prediction of novel putative instances of a motif in a set of biological sequences.\" [ECO:SW, PMID:17117497, PMID:2014171]"
        ],
        "id": [
            "ECO:0005535"
        ],
        "is_a": [
            "ECO:0000202 ! match to sequence model evidence"
        ],
        "name": [
            "machine learning prediction of motif instance evidence"
        ]
    },
    "ECO:0005536": {
        "comment": [
            "This technique is used to predict transcription factor binding sites in gene promoter regions by using a position weight matrix approach. (PMID: 15604457)."
        ],
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-10-09T15:04:35Z"
        ],
        "def": [
            "\"A type of motif similarity evidence where the motif is represented by a position-specific frequency matrix (PSFM) that specifies the frequency of each base at each position of the motif, from which a position-specific scoring matrix (PSSM) can be derived under the framework of a log-likelihood ratio providing a scoring system based on the likelihood of a sequence given the motif, versus a genomic-frequency based null hypothesis.\" [ECO:SW, PMID:10812473]"
        ],
        "id": [
            "ECO:0005536"
        ],
        "is_a": [
            "ECO:0000028 ! motif similarity evidence"
        ],
        "name": [
            "position-specific scoring matrix motif search evidence"
        ],
        "synonym": [
            "\"PSSM\" NARROW []",
            "\"PSSM motif search\" EXACT []"
        ]
    },
    "ECO:0005537": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-10-28T15:31:28Z"
        ],
        "def": [
            "\"A type of reporter gene assay evidence based on the fusion of the xylE gene to a specific promoter for the expression of catechol 2,3-dioxygenase that converts the colorless catechol substrate to yellow 2-hydroxymuconic semialdehyde for identification of the expression of a particular gene.\" [ECO:SW, PMID:2592344]"
        ],
        "id": [
            "ECO:0005537"
        ],
        "is_a": [
            "ECO:0000049 ! reporter gene assay evidence"
        ],
        "name": [
            "xylE reporter gene assay evidence"
        ]
    },
    "ECO:0005538": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-11-17T15:15:50Z"
        ],
        "id": [
            "ECO:0005538"
        ],
        "is_a": [],
        "is_obsolete": [
            "true"
        ],
        "name": [
            "computationally derived logical inference"
        ]
    },
    "ECO:0005539": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-11-17T15:17:47Z"
        ],
        "id": [
            "ECO:0005539"
        ],
        "is_a": [],
        "is_obsolete": [
            "true"
        ],
        "name": [
            "computationally derived logical inference used in automatic assertion"
        ]
    },
    "ECO:0005540": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-11-17T15:24:42Z"
        ],
        "id": [
            "ECO:0005540"
        ],
        "is_a": [],
        "is_obsolete": [
            "true"
        ],
        "name": [
            "computationally derived logical inference from automatic assertion used in automatic assertion"
        ]
    },
    "ECO:0005541": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-11-17T15:32:03Z"
        ],
        "id": [
            "ECO:0005541"
        ],
        "is_a": [],
        "is_obsolete": [
            "true"
        ],
        "name": [
            "computationally derived logical inference from manual assertion used in automatic assertion"
        ]
    },
    "ECO:0005542": {
        "comment": [
            "Processes, pathways and complexes shown by a single experiment should not be annotated with this term but with ECO:0000353 or a child thereof."
        ],
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-12-16T15:32:41Z"
        ],
        "def": [
            "\"A type of biological system reconstruction evidence by experimental evidence from single species that is used in a manual assertion.\" [ECO:SN, PMID:19450514]"
        ],
        "id": [
            "ECO:0005542"
        ],
        "intersection_of": [
            "ECO:0005553 ! biological system reconstruction evidence by experimental evidence from single species",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000352 {is_inferred=\"true\"} ! evidence used in manual assertion",
            "ECO:0005553 ! biological system reconstruction evidence by experimental evidence from single species"
        ],
        "name": [
            "biological system reconstruction evidence by experimental evidence from single species used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0005543": {
        "comment": [
            "Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. The evidence may originate from a combination of several experiments."
        ],
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-12-16T15:45:01Z"
        ],
        "def": [
            "\"A type of biological system reconstruction evidence by experimental evidence from mixed species that is used in a manual assertion.\" [ECO:SN, PMID:22232657]"
        ],
        "id": [
            "ECO:0005543"
        ],
        "intersection_of": [
            "ECO:0005552 ! biological system reconstruction evidence by experimental evidence from mixed species",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000352 {is_inferred=\"true\"} ! evidence used in manual assertion",
            "ECO:0005552 ! biological system reconstruction evidence by experimental evidence from mixed species"
        ],
        "name": [
            "biological system reconstruction evidence by experimental evidence from mixed species used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0005544": {
        "comment": [
            "Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments."
        ],
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-12-18T16:20:49Z"
        ],
        "def": [
            "\"A type of biological system reconstruction evidence based on orthology evidence that is used in a manual assertion.\" [ECO:SN]"
        ],
        "id": [
            "ECO:0005544"
        ],
        "intersection_of": [
            "ECO:0005545 ! biological system reconstruction evidence based on orthology evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0005545 ! biological system reconstruction evidence based on orthology evidence",
            "ECO:0005610 ! biological system reconstruction evidence based on homology evidence used in manual assertion"
        ],
        "name": [
            "biological system reconstruction evidence based on orthology evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0005545": {
        "comment": [
            "Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments."
        ],
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-12-16T15:57:37Z"
        ],
        "def": [
            "\"A type of biological system reconstruction evidence based on homology evidence where the evidence is inferred by orthology from an existing experimentally supported model to a process, pathway or complex in another species.\" [ECO:SN, PMID:15660128]"
        ],
        "id": [
            "ECO:0005545"
        ],
        "is_a": [
            "ECO:0005549 ! biological system reconstruction evidence based on homology evidence"
        ],
        "name": [
            "biological system reconstruction evidence based on orthology evidence"
        ]
    },
    "ECO:0005546": {
        "comment": [
            "Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments."
        ],
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-12-16T16:05:15Z"
        ],
        "def": [
            "\"A type of  biological system reconstruction evidence based on paralogy evidence that is used in a manual assertion.\" [ECO:SN, PMID:15660128]"
        ],
        "id": [
            "ECO:0005546"
        ],
        "intersection_of": [
            "ECO:0005550 ! biological system reconstruction evidence based on paralogy evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0005550 ! biological system reconstruction evidence based on paralogy evidence",
            "ECO:0005610 ! biological system reconstruction evidence based on homology evidence used in manual assertion"
        ],
        "name": [
            "biological system reconstruction evidence based on paralogy evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0005547": {
        "comment": [
            "Functional studies or ligand binding evidence are often used for the reconstruction of biological systems. It does not provide physical interaction evidence but uses proxies such a ligand binding evidences to infer the presence or absence of the complex components."
        ],
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-12-16T16:07:42Z"
        ],
        "def": [
            "\"A type of biological system reconstruction evidence based on inference from background scientific knowledge that is used in a manual assertion.\" [ECO:SN, PMID:17876790]"
        ],
        "id": [
            "ECO:0005547"
        ],
        "intersection_of": [
            "ECO:0005548 ! biological system reconstruction evidence based on inference from background scientific knowledge",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000352 {is_inferred=\"true\"} ! evidence used in manual assertion",
            "ECO:0005548 ! biological system reconstruction evidence based on inference from background scientific knowledge"
        ],
        "name": [
            "biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0005548": {
        "comment": [
            "The available knowledge is usually a combination of partial or weak experimental evidence where either some components are missing or no physical interaction evidence can be found but the system is inferred by similarity to related systems in taxonomically-disparate organisms with experimental evidence. Functional studies or ligand binding evidence are often used for the reconstruction of biological systems. It does not provide physical interaction evidence but uses proxies such a ligand binding evidences to infer the presence or absence of the complex components."
        ],
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-12-18T15:32:15Z"
        ],
        "def": [
            "\"A type of biological system reconstruction based solely on background scientific knowledge of that biological system.\" [ECO:SN, PMID:17876790]"
        ],
        "id": [
            "ECO:0005548"
        ],
        "is_a": [
            "ECO:0000088 ! biological system reconstruction evidence"
        ],
        "name": [
            "biological system reconstruction evidence based on inference from background scientific knowledge"
        ]
    },
    "ECO:0005549": {
        "comment": [
            "Inference may be based on paralogy or orthology of the genome-encoded components and is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments in the same or another species."
        ],
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-12-18T15:49:46Z"
        ],
        "def": [
            "\"A type of biological system reconstruction where the evidence is inferred by homology based on conservation of sequence, function, and composition from an existing experimentally supported model to a process, pathway, or complex.\" [ECO:SN, PMID:15660128]"
        ],
        "id": [
            "ECO:0005549"
        ],
        "is_a": [
            "ECO:0000088 ! biological system reconstruction evidence"
        ],
        "name": [
            "biological system reconstruction evidence based on homology evidence"
        ]
    },
    "ECO:0005550": {
        "comment": [
            "Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments."
        ],
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-12-18T16:24:08Z"
        ],
        "def": [
            "\"A type of  biological system reconstruction evidence based on homology evidence where the evidence is inferred by paralogy from an existing experimentally supported model to a process, pathway, or complex in the same species.\" [ECO:SN, PMID:15660128]"
        ],
        "id": [
            "ECO:0005550"
        ],
        "is_a": [
            "ECO:0005549 ! biological system reconstruction evidence based on homology evidence"
        ],
        "name": [
            "biological system reconstruction evidence based on paralogy evidence"
        ]
    },
    "ECO:0005551": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-12-18T16:34:13Z"
        ],
        "def": [
            "\"A type of biological system reconstruction evidence that uses experimental evidence as support.\" [ECO:SN, PMID:19450514, PMID:22232657]"
        ],
        "id": [
            "ECO:0005551"
        ],
        "is_a": [
            "ECO:0000088 ! biological system reconstruction evidence"
        ],
        "name": [
            "biological system reconstruction evidence by experimental evidence"
        ]
    },
    "ECO:0005552": {
        "comment": [
            "Inference is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. The evidence may originate from a combination of several experiments."
        ],
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-12-18T16:35:13Z"
        ],
        "def": [
            "\"A type of biological system reconstruction evidence where the experimental evidence is derived by using a mix of species used in the same experiment.\" [ECO:SN, PMID:22232657]"
        ],
        "id": [
            "ECO:0005552"
        ],
        "is_a": [
            "ECO:0005551 ! biological system reconstruction evidence by experimental evidence"
        ],
        "name": [
            "biological system reconstruction evidence by experimental evidence from mixed species"
        ]
    },
    "ECO:0005553": {
        "comment": [
            "Processes, pathways and complexes shown by a single experiment should not be annotated with this term but with ECO:0000353 or a child thereof."
        ],
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2015-12-18T16:36:44Z"
        ],
        "def": [
            "\"A type of biological system reconstruction where the experimental evidence is derived by using multiple experiments in a single species.\" [ECO:SN]"
        ],
        "id": [
            "ECO:0005553"
        ],
        "is_a": [
            "ECO:0005551 ! biological system reconstruction evidence by experimental evidence"
        ],
        "name": [
            "biological system reconstruction evidence by experimental evidence from single species"
        ]
    },
    "ECO:0005554": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-02-08T14:30:46Z"
        ],
        "def": [
            "\"A type of sequence alignment evidence where two sequences are aligned to identify regions of similarity that may indicate functional, structural and/or evolutionary relationships between two biological sequences (protein or nucleic acid).\" [ECO:SN, url:http\\://www.ebi.ac.uk/Tools/psa/]"
        ],
        "id": [
            "ECO:0005554"
        ],
        "is_a": [
            "ECO:0000200 ! sequence alignment evidence"
        ],
        "name": [
            "pairwise sequence alignment evidence"
        ]
    },
    "ECO:0005555": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-02-08T14:31:24Z"
        ],
        "def": [
            "\"A type of sequence alignment evidence where three or more biological sequences (protein or nucleic acid) of similar length are aligned to infer homology and the evolutionary relationships between the sequences studied.\" [ECO:SN, url:http\\://www.ebi.ac.uk/Tools/msa/]"
        ],
        "id": [
            "ECO:0005555"
        ],
        "is_a": [
            "ECO:0000200 ! sequence alignment evidence"
        ],
        "name": [
            "multiple sequence alignment evidence"
        ]
    },
    "ECO:0005556": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-02-08T14:59:18Z"
        ],
        "def": [
            "\"A type of multiple sequence alignment evidence that is used in a manual assertion.\" [ECO:SN]"
        ],
        "id": [
            "ECO:0005556"
        ],
        "intersection_of": [
            "ECO:0005555 ! multiple sequence alignment evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000247 {is_inferred=\"true\"} ! sequence alignment evidence used in manual assertion",
            "ECO:0005555 ! multiple sequence alignment evidence"
        ],
        "name": [
            "multiple sequence alignment evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0005557": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-02-08T15:00:50Z"
        ],
        "def": [
            "\"A type of multiple sequence alignment evidence that is used in an automatic assertion.\" [ECO:SN]"
        ],
        "id": [
            "ECO:0005557"
        ],
        "intersection_of": [
            "ECO:0005555 ! multiple sequence alignment evidence",
            "used_in ECO:0000203 ! automatic assertion"
        ],
        "is_a": [
            "ECO:0000248 {is_inferred=\"true\"} ! sequence alignment evidence used in automatic assertion",
            "ECO:0005555 ! multiple sequence alignment evidence"
        ],
        "name": [
            "multiple sequence alignment evidence used in automatic assertion"
        ],
        "relationship": [
            "used_in ECO:0000203 {is_inferred=\"true\"} ! automatic assertion"
        ]
    },
    "ECO:0005558": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-02-08T15:07:47Z"
        ],
        "def": [
            "\"A type of motif discovery evidence that is used in a manual assertion.\" [ECO:SN]"
        ],
        "id": [
            "ECO:0005558"
        ],
        "intersection_of": [
            "ECO:0005531 ! motif discovery evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0005531 ! motif discovery evidence",
            "ECO:0005556 {is_inferred=\"true\"} ! multiple sequence alignment evidence used in manual assertion"
        ],
        "name": [
            "motif discovery evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0005559": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-02-08T15:08:19Z"
        ],
        "def": [
            "\"A type of motif discovery evidence that is used in an automatic assertion.\" [ECO:SN]"
        ],
        "id": [
            "ECO:0005559"
        ],
        "intersection_of": [
            "ECO:0005531 ! motif discovery evidence",
            "used_in ECO:0000203 ! automatic assertion"
        ],
        "is_a": [
            "ECO:0005531 ! motif discovery evidence",
            "ECO:0005557 {is_inferred=\"true\"} ! multiple sequence alignment evidence used in automatic assertion"
        ],
        "name": [
            "motif discovery evidence used in automatic assertion"
        ],
        "relationship": [
            "used_in ECO:0000203 {is_inferred=\"true\"} ! automatic assertion"
        ]
    },
    "ECO:0005560": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-02-08T15:17:05Z"
        ],
        "def": [
            "\"A type of pairwise sequence alignment evidence that is used in a manual assertion.\" [ECO:SN]"
        ],
        "id": [
            "ECO:0005560"
        ],
        "intersection_of": [
            "ECO:0005554 ! pairwise sequence alignment evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000247 {is_inferred=\"true\"} ! sequence alignment evidence used in manual assertion",
            "ECO:0005554 ! pairwise sequence alignment evidence"
        ],
        "name": [
            "pairwise sequence alignment evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0005561": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-02-08T15:17:52Z"
        ],
        "def": [
            "\"A type of pairwise sequence alignment evidence that is used in an automatic evidence.\" [ECO:SN]"
        ],
        "id": [
            "ECO:0005561"
        ],
        "intersection_of": [
            "ECO:0005554 ! pairwise sequence alignment evidence",
            "used_in ECO:0000203 ! automatic assertion"
        ],
        "is_a": [
            "ECO:0000248 {is_inferred=\"true\"} ! sequence alignment evidence used in automatic assertion",
            "ECO:0005554 ! pairwise sequence alignment evidence"
        ],
        "name": [
            "pairwise sequence alignment evidence used in automatic assertion"
        ],
        "relationship": [
            "used_in ECO:0000203 {is_inferred=\"true\"} ! automatic assertion"
        ]
    },
    "ECO:0005562": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-02-09T12:05:29Z"
        ],
        "id": [
            "ECO:0005562"
        ],
        "is_a": [
            "ECO:0000181 ! in vitro assay evidence"
        ],
        "name": [
            "in gel protein kinase assay evidence"
        ]
    },
    "ECO:0005563": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-02-09T12:40:03Z"
        ],
        "def": [
            "\"A type of electrophysiology assay evidence that involves recording trace of the current (pA) going through the analyzed ion channels determined from voltage clamp current recording.\" [ECO:SN, PMID:17938230, url:http\\://www.utdallas.edu/~tres/microelectrode/microelectrodes_ch03.pdf]"
        ],
        "id": [
            "ECO:0005563"
        ],
        "is_a": [
            "ECO:0000164 ! electrophysiology assay evidence"
        ],
        "name": [
            "macroscopic current trace evidence"
        ]
    },
    "ECO:0005564": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-02-09T12:43:07Z"
        ],
        "def": [
            "\"A type of electrophysiology assay evidence that involves measuring the amount of current (pA) going through the ion channels per unit  of membrane.\" [ECO:SN, url:http\\://circres.ahajournals.org/content/102/11/1298.full, url:http\\://www.bem.fi/book/04/04.htm]"
        ],
        "id": [
            "ECO:0005564"
        ],
        "is_a": [
            "ECO:0000164 ! electrophysiology assay evidence"
        ],
        "name": [
            "current density evidence"
        ]
    },
    "ECO:0005565": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-02-09T12:45:42Z"
        ],
        "id": [
            "ECO:0005565"
        ],
        "is_a": [],
        "is_obsolete": [
            "true"
        ],
        "name": [
            "single channel conductance evidence"
        ]
    },
    "ECO:0005566": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-02-09T12:46:54Z"
        ],
        "def": [
            "\"A type of electrophysiology assay evidence that involves the measurement of the current that persists after the fast and slow inactivation of the analyzed channels.\" [ECO:SN, url:http\\://www.nature.com/ncomms/journal/v3/n2/full/ncomms1717.html]"
        ],
        "id": [
            "ECO:0005566"
        ],
        "is_a": [
            "ECO:0000164 ! electrophysiology assay evidence"
        ],
        "name": [
            "sustained current evidence"
        ]
    },
    "ECO:0005567": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-02-09T12:48:17Z"
        ],
        "id": [
            "ECO:0005567"
        ],
        "is_a": [],
        "is_obsolete": [
            "true"
        ],
        "name": [
            "steady state activation curve evidence"
        ]
    },
    "ECO:0005568": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-02-09T12:50:45Z"
        ],
        "id": [
            "ECO:0005568"
        ],
        "is_a": [],
        "is_obsolete": [
            "true"
        ],
        "name": [
            "steady state inactivation curve evidence"
        ]
    },
    "ECO:0005569": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-02-09T12:57:16Z"
        ],
        "id": [
            "ECO:0005569"
        ],
        "is_a": [],
        "is_obsolete": [
            "true"
        ],
        "name": [
            "window current trace evidence"
        ]
    },
    "ECO:0005570": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-02-09T12:58:18Z"
        ],
        "def": [
            "\"A type of electrophysiology assay evidence in which the amount of current remaining after a series of depolarization at different frequencies described as the normalized current related to the time course.\" [ECO:SN, url:http\\://circres.ahajournals.org/content/89/8/700.full]"
        ],
        "id": [
            "ECO:0005570"
        ],
        "is_a": [
            "ECO:0000164 ! electrophysiology assay evidence"
        ],
        "name": [
            "use dependence of inactivation evidence"
        ]
    },
    "ECO:0005571": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-02-12T13:58:32Z"
        ],
        "def": [
            "\"A type of electrophysiology assay evidence that involves recording the change in membrane potential caused by applying specific current to the cell.\" [ECO:SN, url:http\\://www.nature.com/nprot/journal/v4/n8/full/nprot.2009.91.html]"
        ],
        "id": [
            "ECO:0005571"
        ],
        "is_a": [
            "ECO:0000164 ! electrophysiology assay evidence"
        ],
        "name": [
            "current clamp voltage recording evidence"
        ],
        "xref": [
            "birnlex:2278 \"current clamp voltage recording protocol\""
        ]
    },
    "ECO:0005572": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-02-12T14:06:40Z"
        ],
        "def": [
            "\"A type of electrophysiology assay evidence that involves direct measurement of ionic current across a membrane while controlling the membrane potential by maintaining the voltage.\" [ECO:SN, url:http\\://circres.ahajournals.org/content/72/1/91.full.pdf]"
        ],
        "id": [
            "ECO:0005572"
        ],
        "is_a": [
            "ECO:0000164 ! electrophysiology assay evidence"
        ],
        "name": [
            "whole-cell voltage clamp recording evidence"
        ],
        "xref": [
            "birnlex:2281 \"whole-cell voltage clamp recording protocol\""
        ]
    },
    "ECO:0005573": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-02-12T14:07:39Z"
        ],
        "def": [
            "\"A type of electrophysiology assay evidence that involves isolation of a patch of membrane electrically from the external solution and to record current flowing into the patch and this is achieved by pressing a fire-polished glass pipette, which has been filled with a suitable electrolyte solution, against the surface of a cell and applying light suction.\" [ECO:SN, url:http\\://www.utdallas.edu/~tres/microelectrode/microelectrodes_ch04.pdf]"
        ],
        "id": [
            "ECO:0005573"
        ],
        "is_a": [
            "ECO:0000164 ! electrophysiology assay evidence"
        ],
        "name": [
            "cell-attached single-channel recording evidence"
        ],
        "xref": [
            "birnlex:2283 \"cell-attached single-channel recording protocol\""
        ]
    },
    "ECO:0005574": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-02-12T14:16:53Z"
        ],
        "def": [
            "\"A type of electrophysiology assay evidence that involves study of individual ion channels by gently pulling the patch of membrane away from the cell, and the patch remains attached to the pipette with its cytoplasmic surface exposed to the bathing solution.\" [ECO:SN, url:http\\://www.acnp.org/g4/GN401000005/CH005.html]"
        ],
        "id": [
            "ECO:0005574"
        ],
        "is_a": [
            "ECO:0000164 ! electrophysiology assay evidence"
        ],
        "name": [
            "cell-detached inside-out single-channel recording evidence"
        ],
        "xref": [
            "birnlex:2286 \"cell-detached inside-out single-channel recording protocol\""
        ]
    },
    "ECO:0005575": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-02-12T14:17:46Z"
        ],
        "def": [
            "\"A type of electrophysiology assay evidence that involves ion channel analysis by incorporating the ion channels into liposomes suitable for patch-clamping which allows isolation and study of a specific population of channels into that bilayer membrane.\" [ECO:SN, url:http\\://nanion.de/images/stories/papers/Bilayers_lab-on-a-chip08.pdf]"
        ],
        "id": [
            "ECO:0005575"
        ],
        "is_a": [
            "ECO:0000164 ! electrophysiology assay evidence"
        ],
        "name": [
            "reconstituted bilayer single-channel patch recording evidence"
        ],
        "xref": [
            "birnlex:2287 \"reconstituted bilayer single-channel patch recording protocol\""
        ]
    },
    "ECO:0005576": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-02-12T14:18:36Z"
        ],
        "def": [
            "\"A type of electrophysiology assay evidence that involves recording membrane potential by controlling the membrane voltage and measuring the transmembrane current required to maintain that voltage.\" [ECO:SN, url:http\\://neurobio.drexelmed.edu/GaoWeb/Resources/Recording%20_Techniques.pdf, url:http\\://www.acnp.org/g4/GN401000005/CH005.html]"
        ],
        "id": [
            "ECO:0005576"
        ],
        "is_a": [
            "ECO:0000164 ! electrophysiology assay evidence"
        ],
        "name": [
            "voltage clamp current recording evidence"
        ],
        "xref": [
            "birnlex:2279 \"voltage clamp current recording protocol\""
        ]
    },
    "ECO:0005577": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-02-12T14:19:21Z"
        ],
        "def": [
            "\"A type of electrophysiology assay evidence that involves recording the electrical activity of the brain by means of electrodes placed on the surface of the head.\" [ECO:SN, url:http\\://www.jove.com/science-education/5420/electro-encephalography-eeg]"
        ],
        "id": [
            "ECO:0005577"
        ],
        "is_a": [
            "ECO:0000164 ! electrophysiology assay evidence"
        ],
        "name": [
            "electroencephalography recording evidence"
        ],
        "xref": [
            "birnlex:2293 \"electroencephalography recording protocol\""
        ]
    },
    "ECO:0005578": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-02-12T15:20:47Z"
        ],
        "def": [
            "\"A type of microscopy evidence where the images are taken with a higher resolution than the diffraction limit that help in studying nanoscopic sub-cellular structures.\" [ECO:SN, url:http\\://journal.frontiersin.org/article/10.3389/fncel.2015.00007/full]"
        ],
        "id": [
            "ECO:0005578"
        ],
        "is_a": [
            "ECO:0001098 ! microscopy evidence"
        ],
        "name": [
            "super-resolution microscopy evidence"
        ]
    },
    "ECO:0005579": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-02-12T15:38:03Z"
        ],
        "def": [
            "\"A type of immunogold labelling evidence that is used in a manual assertion.\" [ECO:SN]"
        ],
        "id": [
            "ECO:0005579"
        ],
        "intersection_of": [
            "ECO:0000090 ! immunogold labelling evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000090 ! immunogold labelling evidence",
            "ECO:0001232 {is_inferred=\"true\"} ! microscopy evidence used in manual assertion"
        ],
        "name": [
            "immunogold labelling evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0005580": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-02-12T16:04:23Z"
        ],
        "def": [
            "\"A type of flow cytometry evidence that is used in a manual assertion.\" [ECO:SN]"
        ],
        "id": [
            "ECO:0005580"
        ],
        "intersection_of": [
            "ECO:0000268 ! flow cytometry evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000268 ! flow cytometry evidence",
            "ECO:0000314 {is_inferred=\"true\"} ! direct assay evidence used in manual assertion"
        ],
        "name": [
            "flow cytometry evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0005581": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-02-12T16:23:38Z"
        ],
        "def": [
            "\"A type of enzyme-linked immunoabsorbent assay evidence that is used in a manual assertion.\" [ECO:SN]"
        ],
        "id": [
            "ECO:0005581"
        ],
        "intersection_of": [
            "ECO:0000267 ! enzyme-linked immunoabsorbent assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000267 ! enzyme-linked immunoabsorbent assay evidence",
            "ECO:0000269 {is_inferred=\"true\"} ! experimental evidence used in manual assertion"
        ],
        "name": [
            "enzyme-linked immunoabsorbent assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0005582": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-03-02T10:47:25Z"
        ],
        "def": [
            "\"A type of electrophysiology assay evidence where a part of cellular membrane is detached from the cell and whose external face is exposed to the extracellular medium which allows isolating and studying a single or at least a small number of channels into that fragment (patch).\" [ECO:SN, url:http\\://www.nature.com/nprot/journal/v2/n11/full/nprot.2007.403.html]"
        ],
        "id": [
            "ECO:0005582"
        ],
        "is_a": [
            "ECO:0000164 ! electrophysiology assay evidence"
        ],
        "name": [
            "cell-detached outside-out single-channel recording evidence"
        ]
    },
    "ECO:0005583": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-03-02T11:29:32Z"
        ],
        "def": [
            "\"A type of electrophysiology assay evidence that involves inserting the oocyte in a chamber that separates the surface into 3 regions where the top portion of the oocyte membrane is the region that is clamped from which currents are actually recorded while the bottom portion is the region of the oocyte that is cut-open, making it possible to inject current intracellularly through a low resistance pathway.\" [ECO:SN, PMID:9711615]"
        ],
        "id": [
            "ECO:0005583"
        ],
        "is_a": [
            "ECO:0000164 ! electrophysiology assay evidence"
        ],
        "name": [
            "cut-open oocyte voltage clamp recording evidence"
        ]
    },
    "ECO:0005584": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-03-02T11:33:48Z"
        ],
        "def": [
            "\"A type of electrophysiology assay evidence where recording is done on a fragment of the cell membrane (attached to or detached from the cell) using a large tip diameter pipette that allows isolating and studying a small number of channels into that fragment (patch).\" [ECO:SN, url:http\\://jxb.oxfordjournals.org/content/50/Special_Issue/1037.full.pdf]"
        ],
        "id": [
            "ECO:0005584"
        ],
        "is_a": [
            "ECO:0000164 ! electrophysiology assay evidence"
        ],
        "name": [
            "macropatch voltage clamp recording evidence"
        ]
    },
    "ECO:0005585": {
        "comment": [
            "There are significant differences in data analysis and data evaluation if to compare a HTP mass-spec and a regular mass-spec. The regular mass spec method is quite a reliable technique for identifying a protein molecular weight and analysing its post-modifications, other mass-spec applications might require the additional quality controls and more stringent data evaluation. More complex sample mixture is processed, more quality questions should be addressed, such as a sample contamination and sensitivity of the mass spectrometer while using HTP mass-spec.\\nFor HTP mass-spec, the analysis of the small data sets allows to manually assign the spectra to proteins or peptides, but for large data sets only the statistical tools can be used instead."
        ],
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-03-02T12:33:07Z"
        ],
        "def": [
            "\"A type of mass spectrometry evidence where mass-to-charge ratio (m/z) of gas-phase ions is measured which is used to identify and quantify molecules in complex solutions within the framework of high throughput studies.\" [ECO:SN, PMID:20805795]"
        ],
        "id": [
            "ECO:0005585"
        ],
        "is_a": [
            "ECO:0001096 ! mass spectrometry evidence"
        ],
        "name": [
            "high throughput mass spectrometry evidence"
        ]
    },
    "ECO:0005586": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-03-02T12:50:02Z"
        ],
        "def": [
            "\"A type of high throughput mass spectrometry evidence that is used in a manual assertion.\" [ECO:SN]"
        ],
        "id": [
            "ECO:0005586"
        ],
        "is_a": [
            "ECO:0005585 ! high throughput mass spectrometry evidence"
        ],
        "name": [
            "high throughput mass spectrometry evidence used in manual assertion"
        ]
    },
    "ECO:0005587": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-03-17T12:29:45Z"
        ],
        "def": [
            "\"A type of microscopy evidence where sharp images are taken by excluding most of the light from the specimen that is not from the microscope's focal plane, thus allowing better contrast and less haze, which makes it possible to build three-dimensional reconstructions of a volume of the specimen by assembling a series of thin slices taken along the vertical axis.\" [ECO:SN, url:http\\://www.physics.emory.edu/faculty/weeks//lab/papers/ebbe05.pdf]"
        ],
        "id": [
            "ECO:0005587"
        ],
        "is_a": [
            "ECO:0001098 ! microscopy evidence"
        ],
        "name": [
            "confocal microscopy evidence"
        ]
    },
    "ECO:0005588": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-03-17T12:30:27Z"
        ],
        "def": [
            "\"A type of microscopy evidence where imaging of a thin cell or a tissue specimen is performed by full aperture emission of light gathered by the microscope's objective, which maximizes the recorded signal and simultaneously minimizes the required exposure times.\" [ECO:SN, url:http\\://jcb.rupress.org/content/172/1/9.full, url:http\\://www.ncbi.nlm.nih.gov/pmc/articles/PMC3048960/, url:http\\://zeiss-campus.magnet.fsu.edu/articles/livecellimaging/techniques.html]"
        ],
        "id": [
            "ECO:0005588"
        ],
        "is_a": [
            "ECO:0001098 ! microscopy evidence"
        ],
        "name": [
            "wide-field microscopy evidence"
        ]
    },
    "ECO:0005589": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-03-17T12:32:24Z"
        ],
        "def": [
            "\"A type confocal microscopy evidence that is used in a manual assertion.\" [ECO:SN]"
        ],
        "id": [
            "ECO:0005589"
        ],
        "intersection_of": [
            "ECO:0005587 ! confocal microscopy evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0001232 {is_inferred=\"true\"} ! microscopy evidence used in manual assertion",
            "ECO:0005587 ! confocal microscopy evidence"
        ],
        "name": [
            "confocal microscopy evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0005590": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-03-17T12:34:42Z"
        ],
        "def": [
            "\"A type of wide-field microscopy evidence that is used in a manual assertion.\" [ECO:SN]"
        ],
        "id": [
            "ECO:0005590"
        ],
        "intersection_of": [
            "ECO:0005588 ! wide-field microscopy evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0001232 {is_inferred=\"true\"} ! microscopy evidence used in manual assertion",
            "ECO:0005588 ! wide-field microscopy evidence"
        ],
        "name": [
            "wide-field microscopy evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0005591": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-03-17T15:43:00Z"
        ],
        "def": [
            "\"A type of immunogold labelling evidence where electron microscopy technique is used to detect the location of the antibodies labeled with colloidal gold particles.\" [ECO:SN, PMID:4110101]"
        ],
        "id": [
            "ECO:0005591"
        ],
        "is_a": [
            "ECO:0000090 ! immunogold labelling evidence",
            "ECO:0005033 ! electron microscopy evidence"
        ],
        "name": [
            "immunogold labelling electron microscopy assay evidence"
        ]
    },
    "ECO:0005592": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-03-17T16:39:37Z"
        ],
        "def": [
            "\"A type of immunogold labelling electron microscopy assay evidence that is used in a manual assertion.\" [ECO:SN]"
        ],
        "id": [
            "ECO:0005592"
        ],
        "intersection_of": [
            "ECO:0005591 ! immunogold labelling electron microscopy assay evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0005579 {is_inferred=\"true\"} ! immunogold labelling evidence used in manual assertion",
            "ECO:0005591 ! immunogold labelling electron microscopy assay evidence"
        ],
        "name": [
            "immunogold labelling electron microscopy assay evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0005593": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-03-17T17:07:33Z"
        ],
        "def": [
            "\"A type of direct assay evidence that involves the use of antibodies to detect biomolecules.\" [ECO:SN]"
        ],
        "id": [
            "ECO:0005593"
        ],
        "is_a": [
            "ECO:0000002 ! direct assay evidence"
        ],
        "name": [
            "immunodetection assay evidence"
        ]
    },
    "ECO:0005594": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-03-17T17:11:34Z"
        ],
        "def": [
            "\"A type of immunolocalization evidence involving enzymatic detection of a peroxidase tagged antibody.\" [ECO:SN]"
        ],
        "id": [
            "ECO:0005594"
        ],
        "is_a": [
            "ECO:0000087 ! immunolocalization evidence"
        ],
        "name": [
            "immunoperoxidase immunolocalization evidence"
        ]
    },
    "ECO:0005595": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-03-17T17:14:05Z"
        ],
        "def": [
            "\"A type of immunoperoxidase immunolocalization evidence that is used in a manual assertion.\" [ECO:SN]"
        ],
        "id": [
            "ECO:0005595"
        ],
        "intersection_of": [
            "ECO:0005594 ! immunoperoxidase immunolocalization evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0001232 {is_inferred=\"true\"} ! microscopy evidence used in manual assertion",
            "ECO:0005594 ! immunoperoxidase immunolocalization evidence"
        ],
        "name": [
            "immunoperoxidase immunolocalization evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0005596": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-03-17T17:16:38Z"
        ],
        "def": [
            "\"A type of immunoperoxidase immunolocalization evidence where electron microscopy technique is used for enzymatic detection of a peroxidase tagged antibody.\" [ECO:SN]"
        ],
        "id": [
            "ECO:0005596"
        ],
        "is_a": [
            "ECO:0005033 ! electron microscopy evidence",
            "ECO:0005594 ! immunoperoxidase immunolocalization evidence"
        ],
        "name": [
            "immunoperoxidase immunolocalization electron microscopy evidence"
        ],
        "synonym": [
            "\"Immunoperoxidase labelling electron microscopy\" EXACT []"
        ]
    },
    "ECO:0005597": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-03-17T17:23:56Z"
        ],
        "def": [
            "\"A type of immunoperoxidase immunolocalization electron microscopy evidence that is used in a manual assertion.\" [ECO:SN]"
        ],
        "id": [
            "ECO:0005597"
        ],
        "intersection_of": [
            "ECO:0005596 ! immunoperoxidase immunolocalization electron microscopy evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0005595 {is_inferred=\"true\"} ! immunoperoxidase immunolocalization evidence used in manual assertion",
            "ECO:0005596 ! immunoperoxidase immunolocalization electron microscopy evidence"
        ],
        "name": [
            "immunoperoxidase immunolocalization electron microscopy evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0005598": {
        "comment": [
            "The optical filter is used to select the wavelength of excitation light. The detection camera is typically a CCD camera. Both topographical and dynamic information of a sample are obtained."
        ],
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-03-18T11:53:56Z"
        ],
        "def": [
            "\"A type of wide-field microscopy evidence where pure white and ultraviolet light are produced by a mercury lamp, passed through an optical filter (excitation filter), and directed to a sample via a dichroic mirror, followed by detection of the fluorescent light by a camera after it passes through an emission filter.\" [ECO:SN, url:http\\://www.bristol.ac.uk/synaptic/research/techniques/widefield.html]"
        ],
        "id": [
            "ECO:0005598"
        ],
        "is_a": [
            "ECO:0005588 ! wide-field microscopy evidence"
        ],
        "name": [
            "wide-field fluorescence microscopy evidence"
        ]
    },
    "ECO:0005599": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-03-18T11:55:40Z"
        ],
        "def": [
            "\"A type of wide-field fluorescence microscopy evidence where fluorescently tagged antibodies are imaged that are used to bind to their antigens.\" [ECO:SN, url:http\\://journals.plos.org/plosone/article?id=10.1371/journal.pone.0057135]"
        ],
        "id": [
            "ECO:0005599"
        ],
        "is_a": [
            "ECO:0000007 ! immunofluorescence evidence",
            "ECO:0005598 ! wide-field fluorescence microscopy evidence"
        ],
        "name": [
            "immunofluorescence wide-field microscopy evidence"
        ],
        "synonym": [
            "\"wide-field epifluorescence microscopy\" EXACT []"
        ]
    },
    "ECO:0005600": {
        "comment": [
            "This allows visualization of sub cellular distribution of biomolecules of interest."
        ],
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-03-18T12:48:42Z"
        ],
        "def": [
            "\"A type of confocal microscopy evidence where light passes through immunofluorescent-stained tissue sections or cells and then the emitted light passes through the dichroic mirror and is focused onto the pinhole for subsequent detection.\" [ECO:SN, url:http\\://www.physics.emory.edu/faculty/weeks/confocal/, url:https\\://www.hycultbiotech.com/media/wysiwyg/protocol_Immunofluorescence.pdf]"
        ],
        "id": [
            "ECO:0005600"
        ],
        "is_a": [
            "ECO:0000007 ! immunofluorescence evidence",
            "ECO:0005587 ! confocal microscopy evidence"
        ],
        "name": [
            "immunofluorescence confocal microscopy evidence"
        ]
    },
    "ECO:0005601": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-03-18T12:52:04Z"
        ],
        "def": [
            "\"A type of immunofluorescence confocal microscopy evidence that is used in a manual assertion.\" [ECO:SN]"
        ],
        "id": [
            "ECO:0005601"
        ],
        "intersection_of": [
            "ECO:0005600 ! immunofluorescence confocal microscopy evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0005589 {is_inferred=\"true\"} ! confocal microscopy evidence used in manual assertion",
            "ECO:0005600 ! immunofluorescence confocal microscopy evidence"
        ],
        "name": [
            "immunofluorescence confocal microscopy evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ECO:0005602": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-03-18T13:02:29Z"
        ],
        "def": [
            "\"A type of protein expression level evidence where the level of expression of a protein is determined in a cell or tissue extract by western blot technique.\" [ECO:SN]"
        ],
        "id": [
            "ECO:0005602"
        ],
        "is_a": [
            "ECO:0000046 ! protein expression level evidence",
            "ECO:0000112 ! western blot evidence"
        ],
        "name": [
            "protein expression level evidence based on western blot"
        ]
    },
    "ECO:0005603": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-03-18T13:11:37Z"
        ],
        "def": [
            "\"A type of mass spectrometry evidence where one or more protein analyses are performed.\" [ECO:SN]"
        ],
        "id": [
            "ECO:0005603"
        ],
        "is_a": [
            "ECO:0000039 ! protein assay evidence",
            "ECO:0001096 ! mass spectrometry evidence"
        ],
        "name": [
            "protein mass spectrometry evidence"
        ]
    },
    "ECO:0005604": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-03-29T15:40:54Z"
        ],
        "def": [
            "\"A type of experimental evidence where the investigated organism or a potential antagonist is inoculated so as to grow along the diameter line of the agar medium in a Petri dish, then, potentially susceptible microbes (usually bacteria) are streaked perpendicularly to the growth line and inhibition of growth of a test microbe in the vicinity of that line suggests that the antagonist secretes one or more diffusible antibiotics.\" [ECO:SN, url:www.jstor.org/stable/3792785]"
        ],
        "id": [
            "ECO:0005604"
        ],
        "is_a": [
            "ECO:0000006 ! experimental evidence"
        ],
        "name": [
            "cross-streak test evidence"
        ]
    },
    "ECO:0005605": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-03-29T16:02:22Z"
        ],
        "def": [
            "\"A type of experimental evidence where sensitivity/resistance to an antimicrobial agent is tested by impregnating small filter disks with a known concentration of antimicrobial agent which is then placed on a Mueller-Hinton agar plate inoculated with the test organism.\" [ECO:SN, url:http\\://www.cdc.gov/nczved/resources/cholera/ch9.pdf]"
        ],
        "id": [
            "ECO:0005605"
        ],
        "is_a": [
            "ECO:0000006 ! experimental evidence"
        ],
        "name": [
            "disk diffusion test evidence"
        ],
        "synonym": [
            "\"zone of inhibition evidence\" EXACT []"
        ]
    },
    "ECO:0005606": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-03-29T16:59:17Z"
        ],
        "def": [
            "\"A type of experimental evidence where a foreign genetic material is introduced into the cell for study of gene function and regulation and protein function.\" [ECO:SN, url:http\\://ch.promega.com/resources/product-guides-and-selectors/protocols-and-applications-guide/transfection/, url:http\\://www.ncbi.nlm.nih.gov/pmc/articles/PMC2911531/]"
        ],
        "id": [
            "ECO:0005606"
        ],
        "is_a": [
            "ECO:0000006 ! experimental evidence"
        ],
        "name": [
            "cell transfection experiment evidence"
        ]
    },
    "ECO:0005607": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-03-31T15:51:10Z"
        ],
        "def": [
            "\"A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis in swimming organisms is determined by observing the rotational behavior of the organism tethered to a coverslip by their flagella.\" [ECO:SN, PMID:1103143]"
        ],
        "id": [
            "ECO:0005607"
        ],
        "is_a": [
            "ECO:0005021 ! chemotaxis assay evidence"
        ],
        "name": [
            "tethered cell assay evidence"
        ]
    },
    "ECO:0005608": {
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-03-31T16:42:35Z"
        ],
        "def": [
            "\"A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis in swimming organisms is determined by measuring chemotaxis in response to sudden concentration changes from one uniform spatial environment to another.\" [ECO:SN, PMID:4576832]"
        ],
        "id": [
            "ECO:0005608"
        ],
        "is_a": [
            "ECO:0005021 ! chemotaxis assay evidence"
        ],
        "name": [
            "tumble frequency assay evidence"
        ],
        "synonym": [
            "\"Temporal assay\" EXACT []"
        ]
    },
    "ECO:0005609": {
        "comment": [
            "To determine the presence of positive chemotaxis, an attractant is placed in the capillary and the presence of positive chemotaxis is determined based on the amount of organism that accumulated in the capillary.\\nTo determine the presence of negative chemotaxis, a repellent is added to the organismal suspension (but not in the capillary) and the organisms that fled into the capillary are measured."
        ],
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-03-31T16:45:29Z"
        ],
        "def": [
            "\"A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis in swimming organisms is determined (typically bacteria or archaea) by measuring the rate of accumulation of the organism into a capillary.\" [ECO:SN, PMID:4632978]"
        ],
        "id": [
            "ECO:0005609"
        ],
        "is_a": [
            "ECO:0005021 ! chemotaxis assay evidence"
        ],
        "name": [
            "capillary assay evidence"
        ]
    },
    "ECO:0005610": {
        "comment": [
            "Inference may be based on paralogy and/or orthology of the genome-encoded components and is made primarily on functional conservation between the two systems. The sequences and number of genome-encoded components are fairly conserved but some divergence is observed. Evidence may originate from a combination of several experiments."
        ],
        "created_by": [
            "snadendla"
        ],
        "creation_date": [
            "2016-03-31T17:00:03Z"
        ],
        "def": [
            "\"A type of biological system reconstruction evidence based on homology evidence that is used in a manual assertion.\" [ECO:SN]"
        ],
        "id": [
            "ECO:0005610"
        ],
        "intersection_of": [
            "ECO:0005549 ! biological system reconstruction evidence based on homology evidence",
            "used_in ECO:0000218 ! manual assertion"
        ],
        "is_a": [
            "ECO:0000352 {is_inferred=\"true\"} ! evidence used in manual assertion",
            "ECO:0005549 ! biological system reconstruction evidence based on homology evidence"
        ],
        "name": [
            "biological system reconstruction evidence based on homology evidence used in manual assertion"
        ],
        "relationship": [
            "used_in ECO:0000218 {is_inferred=\"true\"} ! manual assertion"
        ]
    },
    "ID:0001601": {
        "created_by": [
            "swolfish"
        ],
        "creation_date": [
            "2015-06-05T14:36:00Z"
        ],
        "def": [
            "\"A type of oligonucleotide microarray evidence that detects binding of a tagged protein to an array of oligonucleotide probes representing potential binding sites.\" [PMID:22146299]"
        ],
        "id": [
            "ID:0001601"
        ],
        "is_a": [
            "ECO:0000093 ! oligonucleotide microarray evidence",
            "ECO:0000136 ! nucleic acid binding evidence"
        ],
        "name": [
            "protein-binding microarray evidence"
        ],
        "synonym": [
            "\"PBM evidence\" EXACT []"
        ]
    },
    "ID:0001603": {
        "created_by": [
            "swolfish"
        ],
        "creation_date": [
            "2015-06-08T11:51:37Z"
        ],
        "def": [
            "\"A type of in vivo assay evidence in which living cells are stained with a vital stain, i.e. dye that can be used on living cells without causing cell death.\" [GOC:MAH]"
        ],
        "id": [
            "ID:0001603"
        ],
        "is_a": [
            "ECO:0000178 ! in vivo assay evidence"
        ],
        "name": [
            "in vivo cell staining evidence"
        ],
        "synonym": [
            "\"vital staining evidence\" RELATED [GOC:MAH]"
        ]
    },
    "ID:0001706": {
        "[Typedef]": [
            "",
            ""
        ],
        "comment": [
            "In the future we may use a more generic relation with weaker domain and range constraints taken from IAO, RO or OBI."
        ],
        "created_by": [
            "kvillanova",
            "mchibucos"
        ],
        "creation_date": [
            "2015-06-09T16:13:20Z",
            "2010-12-09T05:00:20Z"
        ],
        "def": [
            "\"A type of phenotypic evidence based on unsupervised computational examination of a visual mutant phenotype such as abnormalities in organism morphology.\" [ECO:KIM, PMID:25373780]",
            "\"A relation connecting a piece of evidence to an assertion method, where that assertion method is supported by the evidence.\" [GOC:cjm]"
        ],
        "domain": [
            "ECO:0000000 ! evidence"
        ],
        "id": [
            "ID:0001706",
            "used_in",
            "uses"
        ],
        "inverse_of": [
            "used_in ! used_in"
        ],
        "is_a": [],
        "is_obsolete": [
            "true"
        ],
        "name": [
            "computational image-based phenotypic evidence",
            "used_in",
            "uses"
        ],
        "range": [
            "ECO:0000217 ! assertion method"
        ],
        "xref": [
            "ECO:9000000",
            "ECO:9000001"
        ]
    }
}