Unsupervised particle sorting for cryo-EM using probabilistic PCA

This repository contains Matlab implementation of our paper "Unsupervised particle sorting for cryo-EM using probabilistic PCA."

Requirements and Installation

We recommend the following dependencies.

• Matlab 2018b
• ASPIRE Matlab library (http://spr.math.princeton.edu/)

Data

The experimental data sorting used in our paper is on the following dataset.

• EMPIAR-10028 Cryo-EM structure of the Plasmodium falciparum 80S ribosome (https://empiar.pdbj.org/entry/10028/)
• In 'data' folder we have a tagged subset of the above.

Code

• To run our sorting algorithm, configure the model parameters at 'em_test_bench' and run.