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help.md

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Iso-Seq Browser Help.

Visualization parameters

Parameter Description
Annotation Annotations file, in format specified by --format. Reload page to update e.g.example.gtf
Matches Pickle file from MatchAnnot. For multiple files, separate them with comma. Reload page to update. e.g. match1.pickle,match2.pickle
Format Format of annotation file: standard (gtf), alt, pickle
Fasta Folder name for fasta output files of exported data
Transcript height Height of each isoform/transcript
Plot width Width of the plot
Full Full-length read threshold, transcripts with lower full supports will not be displayed
Partial Partial-length read threshold, transcripts with lower partial supports will not be displayed
Group by file Group transcripts by different files (when there are more than one matches file)
Group by similarity Group the transcripts by similarity (using K-Means algorithm)
number of groups Assign transcripts into how many groups

Gene table parameters

Parameter Description
Enter from box / Select from geneTable / Select from marked genes Isoseq-browser allows three way to input gene. One way is to type the gene name into the text box, others are to select gene from the generated geneTable or marked geneTable, which has information of all genes and count of transcripts for that gene.
Gene to visualize The gene to visualize (required)
Go button update the visualization
Rank Transcript Sort the geneTable
Mark Mark genes and save their input parameters for future usage

Bokeh plot tools located to the upper right of the visualization

Tool Description
pan move around the plot
wheel zoom zoom in/out using mouse wheel or touchpad scrolling
tap select one transcript to see its exons
save save the plot as image
reset reset the plot by removing changes made using Bokeh tools such as zoom and move
hover tool hover on exon will display more information, it can be turned off