Only limited number of characters in CURRENT-POP module allowed?

[THccaa]

When I run GPhoCS I get the following output:

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G-Phocs version 1.3.2,  Oct. 2017

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Setting Thread Count to: 4
Reading control settings from file gphocs.ctr...
Error: uneven terms in sample line for pop 1.
Error: expecting to see 110 samples in pop 1, but was able to read only 111.
Error: argument 'Kv29' is not accepted in POP module of CURRENT-POPS.
Error: argument 'PfTa22' is not accepted in POP module of CURRENT-POPS.
Error: uneven terms in sample line for pop 2.
Error: expecting to see 110 samples in pop 2, but was able to read only 111.
Error: argument 'eSk15' is not accepted in POP module of CURRENT-POPS.
Found 7 errors when parsing CURRENT-POPS in control file gphocs.ctr.

My CURRENT-POP module looks like this (first two species only):

CURRENT-POPS-START

		POP-START
				name		Pf
				samples	PfAd16 d PfAd17 d PfAd18 d PfAd19 d PfAd20 d PfAd21 d PfAd22 d PfAd23 d PfAd24 d PfAd25 d PfAd26 d PfAd27 d PfAd28 d PfAd29 d PfAd30 d PfBi11 d PfBi12 d PfBi13 d PfBi14 d PfBi15 d PfBi16 d PfBi17 d PfBi18 d PfBi19 d PfBi20 d PfBi21 d PfBi22 d PfBi23 d PfIh15 d PfIh16 d PfIh17 d PfIh18 d PfIh19 d PfIh20 d PfIh21 d PfIh22 d PfIh23 d PfIh24 d PfIh25 d PfIh26 d PfIh27 d PfIh28 d PfKv16 d PfKv17 d PfKv18 d PfKv19 d PfKv20 d PfKv21 d PfKv22 d PfKv23 d PfKv24 d PfKv25 d PfKv26 d PfKv27 d PfKv28 d PfKv29 d PfKv30 d PfMo15 d PfMo16 d PfMo17 d PfMo18 d PfMo19 d PfMo20 d PfMo21 d PfMo22 d PfMo23 d PfMo24 d PfMo25 d PfMo26 d PfMo27 d PfMo28 d PfMo29 d PfNa16 d PfNa17 d PfNa18 d PfNa19 d PfNa20 d PfNa21 d PfNa22 d PfNa23 d PfNa24 d PfNa25 d PfNa26 d PfNa27 d PfNa28 d PfNa29 d PfNa30 d PfSk16 d PfSk17 d PfSk18 d PfSk19 d PfSk20 d PfSk21 d PfSk22 d PfSk23 d PfSk24 d PfSk25 d PfSk26 d PfSk27 d PfSk28 d PfSk29 d PfSk30 d PfTa13 d PfTa14 d PfTa15 d PfTa16 d PfTa17 d PfTa18 d PfTa19 d PfTa20 d PfTa21 d PfTa22 d PfTa23 d PfTa24 d PfTa25 d PfTa26 d PfTa27 d PfZm14 d PfZm15 d PfZm16 d PfZm17 d PfZm18 d PfZm19 d PfZm20 d PfZm21 d PfZm22 d PfZm23 d PfZm24 d PfZm25 d PfZm26 d PfZm28 d 
		POP-END

		POP-START
				name		Pse
				samples		PeAd01 d PeAd02 d PeAd03 d PeAd05 d PeAd06 d PeAd08 d PeAd09 d PeAd10 d PeAd11 d PeAd12 d PeAd13 d PeAd14 d PeAd15 d PeIh01 d PeIh02 d PeIh03 d PeIh04 d PeIh05 d PeIh06 d PeIh07 d PeIh08 d PeIh09 d PeIh10 d PeIh11 d PeIh12 d PeIh13 d PeIh14 d PeKv01 d PeKv03 d PeKv04 d PeKv05 d PeKv06 d PeKv07 d PeKv08 d PeKv09 d PeKv10 d PeKv11 d PeKv12 d PeKv13 d PeKv14 d PeKv15 d PeSk01 d PeSk02 d PeSk03 d PeSk04 d PeSk05 d PeSk06 d PeSk07 d PeSk08 d PeSk09 d PeSk10 d PeSk11 d PeSk12 d PeSk13 d PeSk14 d PeSk15 d 
		POP-END

I was surprised about the '110 samples' because in pop 1 (Pf) there are more and in pop 2 (Pe) less than 110 samples. Also in pop 1 there is no sample called 'Kv29', it is 'PKv29'. So I counted the number of characters until 'Kv29' and 'PfTa22' appears in pop 1 and until 'eSk15' appears in pop 2 and it is always 111 characters.

Now I wonder if there is actually a character limit or do I have to alter my CURRENT-POPS module some how?

[igronau]

Yes, there is a character limit for the "samples" line. I don't recall now what it is, but regardless of this limit, you should prune your sample set. G-PhoCS is specifically designed to make use of thousands of loci from a few samples per population. Having more than 15 samples per populations is probably excessive and will only bog down the computation without adding much statistical signal. What I suggest is running parallel analyses on subsets of your samples for validation, and using ~10 samples per population in each analysis.