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I am running some tests and I think there is an issue with the ITS gene.
If you see in the output file (PEMA_test_ITS.output.txt), the job stops because the all_samples.fasta file is missing
All the first steps are done! clustering is about to start!
/home1/christina/pema_latest.bds.20250114_122801_867/task.clustering.line_10.id_36.sh: line 4: /mnt/analysis/TEST_ITS_FASTP_VSEARCH/mainOutput/gene_ITS/vsearch/all_samples.fasta: No such file or directory
Task failed:
Program & line : '/home/modules/clustering.bds', line 10
Task Name : ''
Task ID : 'pema_latest.bds.20250114_122801_867/task.clustering.line_10.id_36'
Task PID : '3896'
Task hint : 'sed s/_/;size=/g final_all_samples.fasta > /mnt/analysis/TEST_ITS_FASTP_VSEARCH/mainOutput/gene_ITS/vsearch/all_samples.fasta'
Task resources : 'cpus: 1 mem: -1.0 B timeout: 86400 wall-timeout: 86400'
State : 'ERROR'
Dependency state : 'ERROR'
Retries available : '1'
Input files : '[]'
Output files : '[]'
Script file : '/home1/christina/pema_latest.bds.20250114_122801_867/task.clustering.line_10.id_36.sh'
Exit status : '1'
StdErr (10 lines) :
/home1/christina/pema_latest.bds.20250114_122801_867/task.clustering.line_10.id_36.sh: line 4: /mnt/analysis/TEST_ITS_FASTP_VSEARCH/mainOutput/gene_ITS/vsearch/all_samples.fasta: No such file or directory
Fatal error: /home/modules/clustering.bds, line 12, pos 4. Task/s failed.
pema_latest.bds, line 148 : if ( paramsDereplication{'clusteringAlgo'} == 'algo_vsearch' ) {
pema_latest.bds, line 150 : clusteringVsearch(paramsOfTable, globalVars)
clustering.bds, line 4 : string clusteringVsearch(string{} params, string{} globalVars) {
clustering.bds, line 12 : wait
However, the all_samples.fasta file exists but it is in a different directory, it is in the main output folder /home1/christina/PEMA_215_tests/PEMA_215_ITS_tests/TEST_ITS_FASTP_VSEARCH
I am running some tests and I think there is an issue with the ITS gene.
If you see in the output file (PEMA_test_ITS.output.txt), the job stops because the
all_samples.fasta
file is missingHowever, the

all_samples.fasta
file exists but it is in a different directory, it is in the main output folder/home1/christina/PEMA_215_tests/PEMA_215_ITS_tests/TEST_ITS_FASTP_VSEARCH
So I am assumming there is a copy error?
Here is the parameters file
parameters.txt
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