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ITS in v2.1.5 version #70

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cpavloud opened this issue Jan 14, 2025 · 0 comments
Open

ITS in v2.1.5 version #70

cpavloud opened this issue Jan 14, 2025 · 0 comments
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@cpavloud
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cpavloud commented Jan 14, 2025

I am running some tests and I think there is an issue with the ITS gene.

If you see in the output file (PEMA_test_ITS.output.txt), the job stops because the all_samples.fasta file is missing

All the first steps are done! clustering is about to start!
/home1/christina/pema_latest.bds.20250114_122801_867/task.clustering.line_10.id_36.sh: line 4: /mnt/analysis/TEST_ITS_FASTP_VSEARCH/mainOutput/gene_ITS/vsearch/all_samples.fasta: No such file or directory
Task failed:
	Program & line     : '/home/modules/clustering.bds', line 10
	Task Name          : ''
	Task ID            : 'pema_latest.bds.20250114_122801_867/task.clustering.line_10.id_36'
	Task PID           : '3896'
	Task hint          : 'sed  s/_/;size=/g  final_all_samples.fasta > /mnt/analysis/TEST_ITS_FASTP_VSEARCH/mainOutput/gene_ITS/vsearch/all_samples.fasta'
	Task resources     : 'cpus: 1	mem: -1.0 B	timeout: 86400	wall-timeout: 86400'
	State              : 'ERROR'
	Dependency state   : 'ERROR'
	Retries available  : '1'
	Input files        : '[]'
	Output files       : '[]'
	Script file        : '/home1/christina/pema_latest.bds.20250114_122801_867/task.clustering.line_10.id_36.sh'
	Exit status        : '1'
	StdErr (10 lines)  :
		/home1/christina/pema_latest.bds.20250114_122801_867/task.clustering.line_10.id_36.sh: line 4: /mnt/analysis/TEST_ITS_FASTP_VSEARCH/mainOutput/gene_ITS/vsearch/all_samples.fasta: No such file or directory

Fatal error: /home/modules/clustering.bds, line 12, pos 4. Task/s failed.
pema_latest.bds, line 148 :	if ( paramsDereplication{'clusteringAlgo'} == 'algo_vsearch' ) {
pema_latest.bds, line 150 :	   clusteringVsearch(paramsOfTable, globalVars)
clustering.bds, line 4 :	string clusteringVsearch(string{} params, string{} globalVars) {
clustering.bds, line 12 :	   wait

However, the all_samples.fasta file exists but it is in a different directory, it is in the main output folder /home1/christina/PEMA_215_tests/PEMA_215_ITS_tests/TEST_ITS_FASTP_VSEARCH
image

So I am assumming there is a copy error?

Here is the parameters file
parameters.txt

@cpavloud cpavloud added the bug Something isn't working label Jan 14, 2025
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