Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Not doing what it says on the tin #1

Open
rmcolq opened this issue Dec 10, 2015 · 1 comment
Open

Not doing what it says on the tin #1

rmcolq opened this issue Dec 10, 2015 · 1 comment

Comments

@rmcolq
Copy link
Collaborator

rmcolq commented Dec 10, 2015

OK, so the Readme (which I know is a guideline) says:
Take as input the nanopore assembly, and sam/bam of mapping of illumina reads to the assembly
Take 50kb chunks of the assembly, and pull out the relevant reads
For each chunk, call variants against the reference, and fix up the ref.
Then take the fixed up ref and run standard WG Cortex calling, and again fix up.

At the moment, I believe the cortex_correction.pl script will do a global polishing with bam, but in windows it only works with fq so far.

So, need to:

  1. Handle bam in the cortex_polish.pl script (first step is mapping these reads to the draft assembly. Can we map mapped reads? I should look some more at bwa.
  2. Want do run whole thing from the 'top' script, which is the cortex_polish.pl script (can change name), so should enable it to handle --window_size global or similar, in which case skip mapping step and just do the correction.
@iqbal-lab
Copy link
Owner

I'm happy to change the READMe, wrote it ages ago. I bet bwa can handle bam input

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

2 participants