Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

error: unequal lengths in sequence and overlap file for sequence 2525deca-ece8-4652-8429-19ca02a0aeca! #239

Open
setshabaTaukobong opened this issue Oct 27, 2023 · 1 comment

Comments

@setshabaTaukobong
Copy link

Hi there, I am getting the following issue after running racon:

[racon::Polisher::initialize] loaded target sequences 3.574446 s
[racon::Polisher::initialize] loaded sequences 33.367121 s
[racon::Overlap::transmute] error: unequal lengths in sequence and overlap file for sequence 2525deca-ece8-4652-8429-19ca02a0aeca!
I typed the following command for racon:
racon -m 8 -x -8 -g -6 -w 500 -t 4 /home/staukobong/data/sample_id.trimmed.fastq /home/staukobong/data/sample_id.sam /home/staukobong/data/Assembled/00-assembly/draft_assembly.fasta > sample_idRac.fasta

And I used minimap2 to map my reads to the assembled genome:
minimap2 -a -t 4 /home/staukobong/data/Assembled/00-assembly/draft_assembly.fasta /home/staukobong/data/sample_id.trimmed.fastq > sample_id.sam

Could I please get some assistance on this?

@setshabaTaukobong
Copy link
Author

I used Flye to do the assembly and using Nanopore fastq reads

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

1 participant