semi automatic RNA-Seq analysis
This repository contains a workflow to performs a RNA-Seq analysis created by Laise de Moraes and Joyce Silva (FIOCRUZ-IGM). This README is also available in brazilian portuguese by click here.
Download and install the pipeline from the GitHub repo:
git clone --recursive https://github.com/khourious/sara.git; cd sara
chmod 700 -R INSTALL
bash INSTALLIt is necessary to create the CSV sample sheet. You need create in SHEETS directory.
The csv file name corresponds to the library name and contains in the first line: xxx,xxx,xxx and the next lines: xxxxxxxxxxxxxxxxxxxxxxxxxxxxx -- ATTENTION: NO HEADER!!
xxx,xxx,xxx
semi automatic RNA-Seq analysis
Usage: sara -c <config file name>
-c Name of CSV file that contains the list of the softwares and the experimental groups.
-t Max number of threads (default: all cores).