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Motion correction of image stacks from different focal planes along Z-axis #252

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akatav opened this issue Feb 14, 2020 · 0 comments
Open

Motion correction of image stacks from different focal planes along Z-axis #252

akatav opened this issue Feb 14, 2020 · 0 comments

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@akatav
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akatav commented Feb 14, 2020

Dataset consists of stack of images obtained by 2-photon microscopy of live tissue from different depths along the axial plane.How should SIMA be used to create the imaging stack with such a .TIF file (say, from 1 channel)? Can i still use sima to do motion correction between these frames in one stack ?
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@akatav