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output.md

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Outputs

Reports

Sample results

For each sample, a folder is created with results from various tools, as follows:

  • amr - Predictions of antimicrobial resistance genes
    • abricate - Results from Abricate
    • amrfinderplus - Results from AMFfinderPlus
  • annotation - Gene model predictions
    • prokka - Prokka annotations
  • assembly - The genome assembly and related information
    • busco - Busco analysis of gene space coverage
    • quast - Quast assembly metrics
    • flye/dragonflye/shovill - the assembler output(s)
  • mlst - MLST typing results
  • Plamids - Identification of plasmids from the assembly
  • qc - Basic read QC
    • fastqc - Quality metrics of reads
    • confindr_results - ConfindR contamination check
  • taxonomy - Taxonomic profiling using raw reads
    • kraken2 - Results from Kraken2
  • sample.json - A coarse summary of various sample-level results
Combined results

Some results are computed for all samples of a run, or for all samples belonging to the same species. These results are as follows:

  • cgMLST - core genome MLST calls
    • chewbbaca - Results from Chewbbaca across all samples from the same species, including minimal spanning tree and distance matrix
    • pymlst - Results from pyMLST across all samples from the same species (distance matrix only)
  • AMR
    • Aggregated results from supported antimicrobial resistance gene predictors

QC

MultiQC
  • run_name_multiqc_report.html - Sample-level summary
  • Illumina - QC metrics relating to Illumina data
  • Nanopore - QC metrics relating to Nanopore data
  • Pacbio - QC metrics relating to Pacbio data

Run metrics

pipeline_info

This folder contains the pipeline run metrics

  • pipeline_dag.svg - the workflow graph (only available if GraphViz is installed)
  • pipeline_report.html - the (graphical) summary of all completed tasks and their resource usage
  • pipeline_report.txt - a short summary of this analysis run in text format
  • pipeline_timeline.html - chronological report of compute tasks and their duration
  • pipeline_trace.txt - Detailed trace log of all processes and their various metrics