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Issue for bd-rhapsody setup #18
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I was wondering if you solved the issue. |
I am glad you found UniverSC interesting. |
Thank you for your response. CalledProcessError: Command '['chunk_reads', '--reads-per-fastq', '5000000', '.../SC_RNA_COUNTER_CS/SC_RNA_COUNTER/_BASIC_SC_RNA_COUNTER/CHUNK_READS/fork0/chnk0-uea9b89dd50/files/', 'fastq_chunk', '--martian-args', 'chunk_args.json', '--compress', 'lz4']' returned non-zero exit status 1 It would be helpful if you could make some comments to resolve this problem. |
Thank you for providing valuable tools.
I used the latest universc (v1.2.6) and tried to run setup command for bd-rhapsody-v2.
The result shows "barcode adjust: 0" and the failure for the absence of several files.
I am using cellranger v7.2.0, but the result was obtained even when using cellranger v4.0.0.
My command and messages on the console are as follows:
launch_universc.sh -t bd-rhapsody-v2 --setup
script running in /home/tools/universc/launch_universc.sh...
... script called from /home/tools/universc
Running launch_universc.sh in '/home/tools/universc'
UniverSC Copyright (C) 2019 Tom Kelly; Kai Battenberg
This program comes with ABSOLUTELY NO WARRANTY; for details type 'cat LICENSE'. This is free software, and you are welcome to redistribute it under certain conditions; type 'cat LICENSE' for details.
Cell Ranger is called as third-party dependency and is not maintained by this project. Please ensure you comply with the End User License Agreement for all software installed where applicable; for details type 'cat README.md'.
Using 10x version 2 chemistry to support UMIs
WARNING: conversion was turned on because directory input4cellranger_ was not found
checking if UniverSC is running already
creating .lock file
#####Input information#####
SETUP and exit: true
WARNING: launch_universc.sh will exit once whitelist is converted
FORMAT: bd-rhapsody-v2
BARCODES: /home/tools/universc/whitelists/bd_rhapsody_barcode.txt
WARNING: no FASTQ files were selected, launch_universc.sh will exit after setting up the whitelist
SAMPLE:
LANE:
ID:
DESCRIPTION:
WARNING: no description given, setting to ID value
REFERENCE:
NCELLS: (no cell number given)
CHEMISTRY: SC3Pv2
JOBMODE: local
WARNING: --jobmode "sge" is recommended if running script with qsub
CONVERSION: true
##########
whitelist setup begin
updating barcodes in /home/tools/cellranger-7.2.0/lib/python/cellranger/barcodes for Cell Ranger version cellranger-7.2.0 installed in /home/tools/cellranger-7.2.0/cellranger ...
restoring Cell Ranger
cp: '/home/tools/cellranger-7.2.0/cellranger-cs/cellranger-7.2.0/lib/python/cellranger/webshim/template/navbar.html' Cannot stat: No such file or directory
cp: '/home/tools/cellranger-7.2.0/cellranger-cs/cellranger-7.2.0/lib/python/cellranger/webshim/template/navbar.html' Cannot stat: No such file or directory
sed: /home/tools/cellranger-7.2.0/cellranger-cs/cellranger-7.2.0/lib/python/cellranger/check.py Cannot read: No such file or directory
/home/tools/cellranger-7.2.0/cellranger set for bd-rhapsody-v2
converting whitelist
barcode adjust: 0
whitelist converted
verbose
setup complete
running in local mode (no cluster configuration needed)
setup complete. exiting launch_universc.sh
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