DNA methylation is an epigenetic mechanism used by higher eukaryotes and involved in eg. gene expression, X-Chromosome inactivating, imprinting, and gene silencing of germ-line specific gene and repetitive elements. Here, we propose some materials to learn how to analyze MethylC-seq data.
A deck of slides is available for this topic:
A tutorial with hands-on is available for this topic:
The input datasets for the tutorial will be available soon on Zenodo.
For these tutorials, you can use the dedicated Docker image:
docker run -d -p 8080:80 bgruening/galaxy-methylc-seq-training
It will launch a flavored Galaxy instance available on http://localhost:8080 .
Felix Krueger: DNA methylome analysis using short bisulfite sequencing data
Ryan Lister & Joseph R. Ecker: Finding the fifth base: Genome-wide sequencing of cytosine methylation
Peter W. Laird: Principles and challenges of genome-wide DNA methylation analysis
Alexander Meissner et al Reduced representation bisulfite sequencing for comparative high-resolution DNA methylation analysis
Shawn J. Cokus et al: Shotgun bisulphite sequencing of the Arabidopsis genome reveals DNA methylation patterning
Filipe V. Jacinto et al: Methyl-DNA immunoprecipitation (MeDIP): Hunting down the DNA methylome
Ryan Lister et al: Human DNA methylomes at base resolution show widespread epigenomic differences
This material is maintained by:
- Maintainer 1
- Maintainer 2
For any question related to this topic and the content, you can contact them.
The following individuals have contributed to this training material:
- Name 1
- Name 2