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can this run with single UMI #33

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alexyfyf opened this issue Jun 27, 2024 · 0 comments
Open

can this run with single UMI #33

alexyfyf opened this issue Jun 27, 2024 · 0 comments

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@alexyfyf
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Hi team,

I'm wondering if this pipeline will work if my reads contains only UMI at one end?
What read structure is needed to run this successfully?
My current read looks like [Nanopore primer] -- [barcode] -- [custom primer] -- [ds-cDNA insert] -- [TSO/UMI] -- [barcode] -- [Nanopore primer].
How should I adjust the config to run this?

Thank you.

Alex

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