all reads are short?

[maxstengl]

Hello,
on my test data the pipeline doesn't run past alignment
ERROR - did not find a single cluster passing the min_read threashold!
I assume this is because of all candidate reads are considered short as the umi stats file is written as:

The reads are 100-300 bases long the region in the bed file is
chr12 25398185 25398385 KRAS_Target
min_overlap 0.01 and 0.1 did not work all resulted in:

Reads found: 41943
Reads unmapped: 1959 (4%)
KRAS_Target
Reads found: 0
On target: 66 (0%)
0 concatamers - 0%
66 short - 100%

I changed the min_overlap parameter in the yml file and even tried -1 and 0 but nothing changes the outcome.
We do not use ont UMIs but I adapted the yml file accordingly - but maybe this causes the root problem as no reads to cluster are found?
I guess I am missing something simple - please help !
Thank you,
Daniel