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featureCounts coupled to DESeq2 #76

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julia170 opened this issue Jan 30, 2020 · 2 comments
Closed

featureCounts coupled to DESeq2 #76

julia170 opened this issue Jan 30, 2020 · 2 comments
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enhancement New feature or request

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@julia170
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We run ATAC samples that are not in distinct experimental groups (no replicates) but still would like to make a comparison between the number of reads of each sample in the consensus peaks. However, currently this is not possible because the featureCounts step that does this analysis is coupled to the DESeq2 analysis, which is skipped in the absence of replicates. It would be helpful to separate the two processes, so that the featureCounts step can still be run even if DESeq2 is skipped.

@drpatelh
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Fixed in #77

@drpatelh
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drpatelh commented Jan 31, 2020

@julia170 This should be fixed now. You can test it out by using -r dev in the command. That will use the dev version of the container and the pipeline. You will have to remove your local copy of the pipeline first so the latest version is pulled when you run the command again:
rm -rf ~/.nextflow/assets/nf-core/atacseq/

Note: This wont be fully reproducible until the pipeline is released because changes will most likely be made in the meantime. Be good if you can test it out though 👍

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