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I recognized that the current MetaBAT2 binning discards contigs that do not bin with any other contigs. So when looking at the pipeline output any single-contigs are discarded (e.g. for BUSCO or CAT taxa assignments).
Sounds not too shocking, however I have now a dataset with several really large contigs, e.g. a 4.3Mb sequence that does not appear in the output (MultiQC, BUSCO, CAT). This isn't the way the pipeline should handle the data I think. Unbinned contigs need to be reported more obvious. E.g. with metabat's parameter --unbinned.
The text was updated successfully, but these errors were encountered:
I recognized that the current MetaBAT2 binning discards contigs that do not bin with any other contigs. So when looking at the pipeline output any single-contigs are discarded (e.g. for BUSCO or CAT taxa assignments).
Sounds not too shocking, however I have now a dataset with several really large contigs, e.g. a 4.3Mb sequence that does not appear in the output (MultiQC, BUSCO, CAT). This isn't the way the pipeline should handle the data I think. Unbinned contigs need to be reported more obvious. E.g. with metabat's parameter
--unbinned
.The text was updated successfully, but these errors were encountered: