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FASTQ quality trimming #19

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bovee opened this issue Mar 21, 2018 · 1 comment
Open

FASTQ quality trimming #19

bovee opened this issue Mar 21, 2018 · 1 comment

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@bovee
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bovee commented Mar 21, 2018

If we have unexpectedly large amounts of error k-mers from e.g. the ends of bad FASTQ reads, it can throw distances way off unless the user's setting --min-abun-filter manually. A good way to deal with this would be to allow masking out bad quality score data. A default of filtering all bases below quality 20 (the "bad" cutoff in FastQC; 4 in modern FASTQs, I think?) might also be a good idea?

@tseemann
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tseemann commented Oct 8, 2018

Heng Li often used a cutoff Q=13 which is ~5% error rate. But I think 20 makes more sense ~1%.

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