@@ -82,18 +82,22 @@ complex where a study with a defined set of collected samples can have subsets
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prepared in different ways so we can answer different questions. For example,
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let's imagine a study looking at how different `microbial communities changes
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during mammalian corpse decomposition
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- <https://www.ncbi.nlm.nih.gov/pubmed/26657285> `__. ; thus, your full study design
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+ <https://www.ncbi.nlm.nih.gov/pubmed/26657285> `__; thus, your full study design
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is to collect a set of samples, which you will then process with 16S, 18S and
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ITS primers. This will result in 1 sample and 3 preparation information files,
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`see it in Qiita <https://qiita.ucsd.edu/study/description/10141 >`__.
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- Now, let's imagine other more complex examples:
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+ Now, let's imagine other more complex example:
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+
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1. All of the samples were prepped for 16S and sequenced in two separate
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- MiSeq runs
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- 2. 50 of the samples were prepped for 18S and ITS, and sequenced ina single
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- MiSeq run
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+ MiSeq runs
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+
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+ 2. 50 of the samples were prepped for 18S and ITS, and sequenced in a single
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+ MiSeq run
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+
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3. 50 of the samples were prepped for WGS and sequenced on a single
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- HiSeq run
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+ HiSeq run
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+
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4. 30 of the samples have metabolomic profiles
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To represent this project in Qiita, you will need to create a single
@@ -105,36 +109,36 @@ file. For instance, the data sets described above would require the following
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data and prep information:
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1. All of the samples prepped for 16S and sequenced in two separate
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- MiSeq runs
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+ MiSeq runs
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- a) 1 prep information file describing the two MiSeq runs (use a
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- run\_ prefix column to differentiate between the two MiSeq runs, more
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- on metadata below) where the 100 samples are represented
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- b) the 4-6 fastq raw data files without demultiplexing (i.e., the
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- forward, reverse (optional), and barcodes for each run)
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+ a) 1 prep information file describing the two MiSeq runs (use a
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+ run\_ prefix column to differentiate between the two MiSeq runs, more
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+ on metadata below) where the 100 samples are represented
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+ b) the 4-6 fastq raw data files without demultiplexing (i.e., the
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+ forward, reverse (optional), and barcodes for each run)
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2. 50 of the samples prepped for 18S and ITS, and sequenced in a single
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- MiSeq run
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+ MiSeq run
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- a) prep information files, one describing the 18S and the other describing the
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- ITS preparations
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- b) the 2-3 fastq raw data files (forward, reverse (optional), and
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- barcodes)
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+ a) prep information files, one describing the 18S and the other describing the
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+ ITS preparations
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+ b) the 2-3 fastq raw data files (forward, reverse (optional), and
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+ barcodes)
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3. 50 of the samples prepped for WGS and sequenced on a single HiSeq run
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- a) 1 prep information files describing how the samples were multiplexed
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- b) the 2-3 fastq raw data files (forward, reverse (optional), and
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- barcodes).
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- c) NOTE: We currently do not have a processing pipeline for WGS but
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- should soon.
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+ a) 1 prep information files describing how the samples were multiplexed
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+ b) the 2-3 fastq raw data files (forward, reverse (optional), and
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+ barcodes).
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+ c) NOTE: We currently do not have a processing pipeline for WGS but
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+ should soon.
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4. 30 of the samples with metabolomic profiles
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- a) 1 prep information file. the raw data file(s) from the metabolomic
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- characterization.
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- b) NOTE: We currently do not have a processing pipeline for metabolomics but
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- should soon.
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+ a) 1 prep information file. the raw data file(s) from the metabolomic
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+ characterization.
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+ b) NOTE: We currently do not have a processing pipeline for metabolomics but
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+ should soon.
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Portals
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-------
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