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Differences in results between versions 1.2.0 and 1.6.0 #12

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Niveda-S opened this issue May 31, 2021 · 0 comments
Open

Differences in results between versions 1.2.0 and 1.6.0 #12

Niveda-S opened this issue May 31, 2021 · 0 comments

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@Niveda-S
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Niveda-S commented May 31, 2021

Hi,

I was trying to use the same dataset across two different versions of probatch 1.2.0 and 1.6.0. There sees to be a huge difference in the results between the two versions.
I. For example, for the same dataset, the PVCA using version 1.2.0 shows batch corrections but using 1.6.0 shows no batch correction:
a) Version 1.2.0
Before Correction:
image
After Correction (Combat+Loess):
image
b) Version 1.6.0
Before Correction:
image
After Correction (Combat+Loess):
image
II. Similarly, the PCAs looks super different:
a) Version 1.2.0
Before Correction:
image
After Correction (Combat+Loess):
image
b) Version 1.6.0
Before Correction:
image
After Correction (Combat+Loess):
image

This is only for combat+loess results. Running just combat produces identical results. What is the reason for the difference? It looks like version 1.6.0 does not do batch correction at all. Should I be using version 1.2.0?

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